1.Isolation and Culture of Human Embryonic Stem-like Cells from Abnormal Blastocysts.
Chun Kyu LIM ; Ji Hye SUNG ; Jong Hyuk PARK ; Sun Jong KIM ; Hyun Soo YOON ; Mi Kyoung KOONG ; Jin Hyun JUN
Korean Journal of Fertility and Sterility 2003;30(4):293-298
No abstract available.
Blastocyst*
;
Humans*
2.Effect of insulin on development of mouse preimplantation embryos.
Jang Heub KIM ; Woong Shik AHN ; Seog Nyeon BAE ; Young Oak LEW ; Yong Taik LIM ; Jin Hong KIM ; Jin Woo LEE ; Hun Young LEE
Korean Journal of Obstetrics and Gynecology 1993;36(7):928-937
No abstract available.
Animals
;
Blastocyst*
;
Insulin*
;
Mice*
3.Effects of Media Volume on Blastocyst Formation, Cell Numbersand ICM Proportion in Mouse Two-cell Embryos.
Korean Journal of Fertility and Sterility 2004;31(1):1-7
No abstract available.
Animals
;
Blastocyst*
;
Embryonic Structures*
;
Mice*
4.Development to Hatching Blastocysts and Cell Allocation to the Inner Cell Mass and Trophectoderm of Pig In Vitro Embryos as Affected by Amino Acids and Serum.
Sang Jun UHM ; Eun Young KIM ; Myo Kyung KIM ; Bong Kyung YI ; Hyeon Sook LEE ; Teoan KIM ; San Hyun YOON ; Sepill PARK ; Kil Saeng CHUNG ; Jin Ho LIM
Korean Journal of Fertility and Sterility 1997;24(2):241-251
No abstract available.
Amino Acids*
;
Blastocyst*
;
Embryonic Structures*
5.Effects of Exposure of Propidium Iodide and Bisbenzimide on Differential Staining of Mouse Blastocysts.
Kee Sang PARK ; Sung Baek PARK ; Taek Hoo LEE ; Sang Sik CHUN ; Hai Bum SONG
Korean Journal of Fertility and Sterility 2002;29(4):317-322
OBJECTIVE: These experiments were conducted to investigate the optimal expose length of propidium iodide (PI) and bisbenzimide on differential staining of mouse blastocysts. MATERIALS AND METHODS: A total 964 blastocysts (early~hatched) was exposed to PI (n=831) (group I:
6.Effects of BSA, glucose and phosphate on 2-cell block and blastocyst development of 1-cell mouse embryos during in vitro culture.
Sun Haeng KIM ; Yong Ho LEE ; Jung Jae LEE ; Il Joong AN ; Gee Hoon JANG ; Tak KIM
Korean Journal of Obstetrics and Gynecology 2000;43(12):2274-2282
No abstract available.
Animals
;
Blastocyst*
;
Embryonic Structures*
;
Glucose*
;
Mice*
7.Moral status of preimplantation embryos.
Korean Journal of Obstetrics and Gynecology 2008;51(3):286-289
here have been continuing challenges to devaluate the moral status of early embryo. The preembryo-embryo distinction was mainly based on the lack of individuation. The term of preembryo was introduced by a frog embryologist and then was literally spread around the world because of policy reasons. The term "preembryo" has not yet been used in most medical textbooks including textbooks of human embryology. Preembryo is one period of continuum during human development and therefore should be regarded as valuable as an early form of human life. Preimplantation embryo is more accurate term for the early embryo.
Blastocyst
;
Embryonic Structures
;
Human Development
;
Humans
;
Individuation
8.Moral status of preimplantation embryos.
Korean Journal of Obstetrics and Gynecology 2008;51(3):286-289
here have been continuing challenges to devaluate the moral status of early embryo. The preembryo-embryo distinction was mainly based on the lack of individuation. The term of preembryo was introduced by a frog embryologist and then was literally spread around the world because of policy reasons. The term "preembryo" has not yet been used in most medical textbooks including textbooks of human embryology. Preembryo is one period of continuum during human development and therefore should be regarded as valuable as an early form of human life. Preimplantation embryo is more accurate term for the early embryo.
Blastocyst
;
Embryonic Structures
;
Human Development
;
Humans
;
Individuation
10.Effects of the Stepwise Exposure Treatments Before Freezing on the Survival Capacity of the Frozen-Thawed Mouse Mature Oocytes by Vitrification or Ultra-Rapid Freezing.
Sang Woo KIM ; Young Ah LEE ; Man Soo YOON ; Kyu Sup LEE ; Jae Ik LEE ; Mi Kyung KIM
Korean Journal of Fertility and Sterility 2000;27(2):191-200
OBJECTIVE: This study was carried out to compare the effects of the stepwise exposure treatments on the morphological normality, fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing and to use as a fundamental data for the cryopreservation of human oocytes. MATERIALS AND METHODS: The morphological normality and fertilization rates of the vitrified and ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were observed. After choosing the 3step exposure treatment groups, we observed the morphological normality and fertilization, blastocyst formation rate vitrified and ultra-rapid frozen mouse mature oocytes. RESULTS: The morphological normality and fertilization rates of the vitrified mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 75%, 85%, 88% and 58%, 61%, 54% respectively. There were no significant differences among treatments (p>0.05). The morphological normality and fertilization rates of the control was 92% and 65%. There were no significant differences in fertilization rate among control and treatments (p>0.05). The morphological normality and fertilization rates of the ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 83%, 83%, 84% and 75%, 63%, 56% respectively. There were no significant differences among treatments (p>0.05). The morphological normality and fertilization rate of the control was 95% and 67%. There were no significant differences among control and treatments (p>0.05). The morphological normality and fertilization rate of the vitrified or ultra-rapid frozen mouse mature oocytes after 3step exposure treatment were 69% and 75%, respectively. The blastocyst formation rate was 60% and 57%. The results did not differ significantly between vitrification and ultra-rapid freezing (p>0.05). CONCLUSION: As known in the above results, there were no significant differences in the fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing among the control and treatments. It is suggested that vitrification and ultra-rapid freezing method were effective for the cryopreservation of mouse mature oocytes.
Animals
;
Blastocyst
;
Cryopreservation
;
Fertilization
;
Freezing*
;
Humans
;
Mice*
;
Oocytes*
;
Vitrification*
Result Analysis
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