1.Isolation and Culture of Human Embryonic Stem-like Cells from Abnormal Blastocysts.
Chun Kyu LIM ; Ji Hye SUNG ; Jong Hyuk PARK ; Sun Jong KIM ; Hyun Soo YOON ; Mi Kyoung KOONG ; Jin Hyun JUN
Korean Journal of Fertility and Sterility 2003;30(4):293-298
No abstract available.
Blastocyst*
;
Humans*
2.Effect of insulin on development of mouse preimplantation embryos.
Jang Heub KIM ; Woong Shik AHN ; Seog Nyeon BAE ; Young Oak LEW ; Yong Taik LIM ; Jin Hong KIM ; Jin Woo LEE ; Hun Young LEE
Korean Journal of Obstetrics and Gynecology 1993;36(7):928-937
No abstract available.
Animals
;
Blastocyst*
;
Insulin*
;
Mice*
3.Effects of Exposure of Propidium Iodide and Bisbenzimide on Differential Staining of Mouse Blastocysts.
Kee Sang PARK ; Sung Baek PARK ; Taek Hoo LEE ; Sang Sik CHUN ; Hai Bum SONG
Korean Journal of Fertility and Sterility 2002;29(4):317-322
OBJECTIVE: These experiments were conducted to investigate the optimal expose length of propidium iodide (PI) and bisbenzimide on differential staining of mouse blastocysts. MATERIALS AND METHODS: A total 964 blastocysts (early~hatched) was exposed to PI (n=831) (group I:
4.Effects of Media Volume on Blastocyst Formation, Cell Numbersand ICM Proportion in Mouse Two-cell Embryos.
Korean Journal of Fertility and Sterility 2004;31(1):1-7
No abstract available.
Animals
;
Blastocyst*
;
Embryonic Structures*
;
Mice*
5.Development to Hatching Blastocysts and Cell Allocation to the Inner Cell Mass and Trophectoderm of Pig In Vitro Embryos as Affected by Amino Acids and Serum.
Sang Jun UHM ; Eun Young KIM ; Myo Kyung KIM ; Bong Kyung YI ; Hyeon Sook LEE ; Teoan KIM ; San Hyun YOON ; Sepill PARK ; Kil Saeng CHUNG ; Jin Ho LIM
Korean Journal of Fertility and Sterility 1997;24(2):241-251
No abstract available.
Amino Acids*
;
Blastocyst*
;
Embryonic Structures*
6.Effects of BSA, glucose and phosphate on 2-cell block and blastocyst development of 1-cell mouse embryos during in vitro culture.
Sun Haeng KIM ; Yong Ho LEE ; Jung Jae LEE ; Il Joong AN ; Gee Hoon JANG ; Tak KIM
Korean Journal of Obstetrics and Gynecology 2000;43(12):2274-2282
No abstract available.
Animals
;
Blastocyst*
;
Embryonic Structures*
;
Glucose*
;
Mice*
7.Moral status of preimplantation embryos.
Korean Journal of Obstetrics and Gynecology 2008;51(3):286-289
here have been continuing challenges to devaluate the moral status of early embryo. The preembryo-embryo distinction was mainly based on the lack of individuation. The term of preembryo was introduced by a frog embryologist and then was literally spread around the world because of policy reasons. The term "preembryo" has not yet been used in most medical textbooks including textbooks of human embryology. Preembryo is one period of continuum during human development and therefore should be regarded as valuable as an early form of human life. Preimplantation embryo is more accurate term for the early embryo.
Blastocyst
;
Embryonic Structures
;
Human Development
;
Humans
;
Individuation
8.Moral status of preimplantation embryos.
Korean Journal of Obstetrics and Gynecology 2008;51(3):286-289
here have been continuing challenges to devaluate the moral status of early embryo. The preembryo-embryo distinction was mainly based on the lack of individuation. The term of preembryo was introduced by a frog embryologist and then was literally spread around the world because of policy reasons. The term "preembryo" has not yet been used in most medical textbooks including textbooks of human embryology. Preembryo is one period of continuum during human development and therefore should be regarded as valuable as an early form of human life. Preimplantation embryo is more accurate term for the early embryo.
Blastocyst
;
Embryonic Structures
;
Human Development
;
Humans
;
Individuation
10.The Influences of Different Composition of Glucose and Pyruvate on In Vitro Development of Mouse Preimplantation Embryos in Medium with Glutamine.
Ju Hwan KIM ; Young Seok SEO ; Song Ki CHOI ; Oh Seong LEE ; Hai Bum SONG ; Kil Chun KANG ; Ki Hwan LEE
Korean Journal of Obstetrics and Gynecology 2004;47(1):118-125
OBJECTIVE: The purpose of this study was to examine the effects on in vitro development of early preimplantation mouse embryos in DMEM medium with glutamine which was controlled by different composition of glucose and pyruvate. METHODS: Four hundred and nineteen mouse 2-cell embryos were cultured in four different media with different composition of glucose and pyruvate for 96 hours. The DMEM-G contained L-glutamine for energy sources was used for control group. Group I embryos were cultured in the medium that mixed one volume of DMEM-GGP contained L-glutamine, D-glucose and sodium pyruvate for energy sources with three volume of DMEM-G, and group II embryos were cultured in the medium that mixed with same volume of DMEM-G and DMEM-GGP, and group III embryos were cultured in DMEM-GGP. RESULTS: At 24 hours, the development into >or=3-cell was significantly higher (p<0.05) in group I (93.3%) than control (84.6%). The development into >or=8-cell was significantly higher in group I (73.1%) than control (44.2%), group II (59.6%) and III (45.8%), and also group II was significantly higher than control and group III. At 48 hours, the development into >or=morula was significantly higher in group I (90.4%) and II (86.5%) than control (73.0%). However, the development into blastocyst, in group III (15.0%) was significantly lower than control, group I and II. At 72 hours, the development into >or=expanded blastocyst was significantly higher in group I (69.2%) than group III (47.7%), and total blastocyst was significantly higher in group I (80.8%) than control (66.3%) and group III (67.3%). At 96 hours, the development into >or=hatching blastocyst was significantly higher in group I (78.8%) than control (61.5%) and group III (57.9%), and also, total blastocyst was significantly higher in group I (85.6%) than control (69.2%) and group III (72.0%). CONCLUSION: The development of early preimplantation mouse embryos cultured in group I medium that mixed one volume of DMEM-GGP with three volume of DMEM-G was better than other groups during the culture period.
Animals
;
Blastocyst*
;
Embryonic Structures
;
Glucose*
;
Glutamine*
;
Mice*
;
Pyruvic Acid*
;
Sodium
Result Analysis
Print
Save
E-mail