No abstract available.
Blastocyst* ; Humans*

No abstract available.
Animals ; Blastocyst* ; Insulin* ; Mice*

No abstract available.
Animals ; Blastocyst* ; Embryonic Structures* ; Mice*

No abstract available.
Amino Acids* ; Blastocyst* ; Embryonic Structures*

OBJECTIVE: These experiments were conducted to investigate the optimal expose length of propidium iodide (PI) and bisbenzimide on differential staining of mouse blastocysts. MATERIALS AND METHODS: A total 964 blastocysts (early~hatched) was exposed to PI (n=831) (group I: Animals ; Bisbenzimidazole* ; Blastocyst* ; Mice* ; Propidium*

No abstract available.
Animals ; Blastocyst* ; Embryonic Structures* ; Glucose* ; Mice*

here have been continuing challenges to devaluate the moral status of early embryo. The preembryo-embryo distinction was mainly based on the lack of individuation. The term of preembryo was introduced by a frog embryologist and then was literally spread around the world because of policy reasons. The term "preembryo" has not yet been used in most medical textbooks including textbooks of human embryology. Preembryo is one period of continuum during human development and therefore should be regarded as valuable as an early form of human life. Preimplantation embryo is more accurate term for the early embryo.
Blastocyst ; Embryonic Structures ; Human Development ; Humans ; Individuation

here have been continuing challenges to devaluate the moral status of early embryo. The preembryo-embryo distinction was mainly based on the lack of individuation. The term of preembryo was introduced by a frog embryologist and then was literally spread around the world because of policy reasons. The term "preembryo" has not yet been used in most medical textbooks including textbooks of human embryology. Preembryo is one period of continuum during human development and therefore should be regarded as valuable as an early form of human life. Preimplantation embryo is more accurate term for the early embryo.
Blastocyst ; Embryonic Structures ; Human Development ; Humans ; Individuation

Humans ; Consensus ; Blastocyst ; Embryonic Development ; Fertilization in Vitro

The present study was to assess the effect of ultrarapid freezing on the development of 1-cell mouse zygote using cryoprotectants, DMSO (dimethyl sulfoxide) or PROH (1,2-propanediol). We investigated the effect of the type and concentration of cryoprotectant, and of the temperature and time of prefreezing equilibration on their capacity to develop to the blastocyst stage in vitro. The concenration, the equilibration temperature, and the exposure time seemed to serve as an important factor in ultrarapid freezing of 1-cell mouse zygotes. In addition to the exposure time and the concentration of cryoprotectant appeared to play a key role in the development of the embryo. In general, the development of the embryo was more effective at 3degrees C than 23degrees C and 4.5 M than 3 M for 3 to 5 minutes. At 23degrees C the development of the embryo was stimulated by DMSO while at 3degrees C it was stimulated by PROH. Thus it has been suggested that there exists a correlation between the concentration of cryoprotectants and exposure time in the development of the embryo. In conclusion, we found that for ultrarapid freezing of mouse 1-cell embryos in DMSO, or PROH-based solution, viability shown optimum depending on the cryoprotectant, the concentration of the cryoprotectant and on the temperature and the duration of equilibration.
Animals ; Blastocyst ; Dimethyl Sulfoxide ; Embryonic Structures ; Freezing* ; Mice* ; Zygote*