Objective To establish the reference interval of 3-14 years old children in Nanhai district of Foshan city .Methods 2 032 cases of 3-14 years old healthy children were detected and analyzed using MINDRAY BC-5800 automatic blood cell analy-zer .The levels of WBC ,RBC ,Hb ,PLT ,Hct ,MCV ,MCH ,MCHC ,RDW were analyzed .Results The levels of WBC ,RBC ,Hb , PLT ,Hct ,MCV ,MCH ,MCHC ,RDW in 2 031 cases were non normal distribution .The levels of Hb ,Hct had no statistical signifi-cance between male children and female children (P>0 .05);The levels of WBC ,RBC ,PLT ,MCV ,MCH ,MCHC ,RDW had statis-tical significance between male children and female children (P<0 .05) .Conclusion It is very important to establish the reference interval of 3-14 years old children in Nanhai district of Foshan city .

Objective To investigate the effects of congenital ptosis on refractive power of the corneal surface.Methods Forty-five patients (90 eyes) with congenital ptosis in the First Affiliated Hospital of Kunming Medical University were examined from October 2013 to June 2015.Corneal topographic analysis was performed in both the ptosis and the normal eyes.The comparison between the ptotic eye and the normal eye was done according to the topographic results.Results Among 90 eyes,55 were ptotic and 35 were normal.Amblyopia was detected in 21 ptotic eyes (38.2％).No correlation was found between amblyopia and the severity of ptosis (x 2=2.617,P＞0.05).Ptotic eye showed an asymmetrical bow tie pattern (23 eyes) and an irregular pattern (32 eyes) on corneal photography and the maximum refractive power occurred at the inferior quadrant of the corneal horizontal axis.Difference value of the average refractive power of the cornea between lower side and upper side in ptotic eyes was higher than that of the normal eyes at 5mm and 7mm diameter areas (P＜0.01) except for 3mm diameter area (P＞ 0.05).Conclusion Congenital ptosis may lead to the change of refractive power of the local corneal surface.Amblyopia has no correlation with the severity of ptosis.

0.05).Conclusions:The integrated medicine can regulate monoamine neurotransmitter to some extent.

Objective To investigate the traits of anxiety and depression of psychological stress insomniacs and the relationship to personality and behavior type.Methods The anxiety and depression as well as personality and behavior type of 56 psychological stress insomniacs were assessed with Self-Rating Anxiety Scale(SAS) and Self-Rating Depression Scale(SDS) as well as Type A Behavior Pattern Scale(TABP).Results Compared with domestic norm,scores of SAS and SDS of psychological stress insomniacs were significantly higher(P<0.01).According to classification standard of SAS and SDS,54 patients(96.4%) were with slight or moderate anxiety and 50 patients(89.3%) with slight or moderate depression.The general distribution of severity of anxiety and depression was no difference(P>0.05).The number of type A-behavior psychological stress insomniacs was 22,obviously more than type A(8 cases),type M(5 cases),type B-(13 cases) and type B(8 cases).Conclusion Psychological stress insomniacs have obvious anxiety and depression.

Objective To investigate the progress and effectiveness of water-improving defluoridation projects comprehensively in control of fluorosis in drinking-water-borne endemic fluorosis areas in Hubei Province,and to provide a scientific basis for making strategies in prevention and control of endemic fluorosis.Methods All villages affected by fluorosis in drinking-water-borne endemic fluorosis areas were selected in Hubei Province,in which the progress and effectiveness of water-improving and defluoridation projects were investigated in 2015.Those villages were chosen to determine fluoride content of drinking water,and to check the prevalence of dental fluorosis of children 8 to 12 years old.Water fluoride content was tested with fluoride ion-selective electrode,and dental fluorosis was diagnosed by the Dean method.Results The water improvement projects were completed in 197 villages in the 30 disease affected counties (cities) with accomplishment rate 100.00％ (197/197),geometric mean of water fluorine 0.36 mg/L,qualified rate of water fluorine 100.00％ (197/197) and normal operation rate of the water-improvement projects 95.94％ (189/197).Totally 14 856 children aged 8-12 were examined their dental fluorosis.The prevalence rate was 1.78％ (265/14 856) and the dental fluorosis index was 0.04,which meant epidemic strength was negative.Totally 29 out of the 30 disease affected counties (cities) had met the disease control standards.Conclusion The prevalence of drinking-water-borne endemic fluorosis in Hubei Province has been under control,but management and monitoring of the water improvement projects still should be strengthened.

Objective: To analyze the association of thrombocytosis with the prognosis of patients with gastric cancer.Methods: The clinical materials of 782 patients with gastric cancer who underwent initial surgery in our hospital between January 1995 and December 1999 were retrospectively analyzed.Kaplan- Meier and Log-Rank test were used to analyze the data.Prognostic factors were analyzed by multivariate Cox proportional hazards model.Results: Thrombocytosis oc-curred in 11.4% (87/782) patients.The platelet level was not significantly different among patients of different gender, tumor stage, and histological differentiation (P>0.05).However, a significant difference was observed in the platelet level among patients with different age and surgical approach (P<0.05).The 1-, 3-, and 5-year survival rates were 75.0%, 40.1% and 28.9% in patients without thrombocytosis and 52.8%, 16.9%, and 13.5% in patients with thrombocytosis (P=0.002).Univari-ate analysis showed that histological differentiation, pathological stage, surgical approach and thrombocytosis significantly affected the survival of patients.While age and gender had no significant impact on patient survival.Multivariate analysis showed that pathological stage, surgical approach, and thrombocytosis were independent prognostic factors for gastric can-cer.The relative risk of death of patients with thrombocytosis was elevated by 1.454 times (RR=1.454, 95% CI: 1.135～1.861, P=0.005).Conclusion: Thrombocytosis is an independent prognostic indicator for the survival of initially treated pa-tients with gastric cancer.

Objective To investigate the diagnostic value of serum human epididymis protein 4 (HE4 ) for ovarian cancer . Methods Electrochemiluminescence immunoassay was employed to detect the serum HE 4 of 91 patients with ovarian cancer (ovari-an cancer group) ,69 patients with benign ovarian disease(benign group) and 60 healthy people(control group) .Area under the curve(AUC ) of receiver operator characteristic (ROC ) curve for HE4 was calculated .Results Serum HE4 level [(146 .81 ± 81 .29)pmol/L] of patients in ovarian cancer group was significantly higher than those in control group [(43 .16 ± 25 .64) pmol/L] and benign group[(59 .86 ± 39 .87) pmol/L](P<0 .01) .Set serum HE4≥140 pmol/L as positive cutoff value ,the diagnostic sensi-tivity of serum HE4 detection for ovarian cancer was 82 .4% (75/91) ,specificity 89 .9% (62/69) and diagnostic efficiency 85 .6% . Serum HE4 levels of patients with serous adenocarcinoma ,endometrial adenocarcinoma or other ovarian cancer were markedly high-er than that with mucinous adenocarcinoma (P<0 .01) .Serum HE4 levels of patients with stage Ⅲ ,Ⅳ ovarian cancer were obvious-ly higher than those with Ⅰ ,Ⅱ stage(P<0 .01) .Conclusion HE4 detection has clinical value for the auxiliary diagnosis of ovarian cancer .

Objective To investigate the effects of different doses Luoshi-Neiyi prescription in expression of E-cadherin of endometriosis model SD rats. Methods The female rats with SPF class SD were randomly divided into six groups: high-dose group, medium-dose group, low-dose group, model group, sham operation group and blank group, 15 of each group. Apart from the sham operation group and the blank group, the rats in the remaining group used abdominal cavity injection to establish the model of the endoheterosis rat model. After the successful establishment of the model, the mice were given high, medium,low dose and the sterilization water for 8 weeks. After stopping the drug, the left uterus of the rats was taken from the left uterus of each group in the fourth day of pregnancy to detect the E-cadherin by Western Blotting. Results Compared with the model group, the expression of E-cadherin (0.76 ± 0.26, 0.96 ± 0.33 vs. 0.24 ± 0.05) in the medium-dose and high dose groups significantly incresed (P<0.05). There was no differentence in the mediumdose and highdose groups (P<0.05). Conclusions The medium dose and high dose of Luoshi-Neiyi prescription can up-regulate the expression of E-cadherin in Endometriosis model rats.

Following rapid social and economic development over the past several decades,pollution by heavy metals has been both serious and widespread in many areas of the world,including China.The situations of heavy metal pollution in China were reviewed,and the health risk and control policy of such pollution were also analyzed and discussed in present paper.