OBJECTIVE To study the distribution and drug-resistance status of pathogens in surgically infective specimen in Renmin Hospital of Qichun County and provide the reference for clinically reasonable use of antibiotics.METHODS The infective specimen were collected to perform bacterial culture.The routine methods were adopted to identify pathogenic bacterium.Susceptibility testing was performed by K-B method.RESULTS Among 322 strains from surgically infective specimen,Staphylococcus aureus,Escherichia coli and Pseudomonas aeruginosa,ranking the top three,accounted for 25.2%,15.8% and 13.0%,respectively.The detection rate of meticillin-resistant S.aureus(MRSA) was 32.1%.And that of meticillin-resistant coagulase negative Staphylococcus(MRCNS) was 33.3%.That of extended spectrum ?-lactamases(ESBLs) producer in E.coli and Klebsiella pneumoniae was 34.5%.Various pathogenic bacterium had been resistant to antibiotics to different extents.100.0% of Gram-positive cocci were sensitive to vancomycin.The sensitivity rate to imipenem in Gram-negative bacilli was 19.0-100.0%.CONCLUSIONS Drug resistance variesd from pathogenic bacterium in surgical infection.The clinician should reasonably choose antibiotics according to results of bacterial culture to avoid occurrence and prevalence of drug-resistance bacterium.

Objective To conduct a descriptive epidemiological study on thyroid cancer incidence in the urbanarea of Tianjin during 2002 - 2006 and to analyze the secular trend of incidences based on previous data of 26 years and those from Shanghai and the United States. Methods Annual percent change (APC%) of thyroid cancer incidence and the rank of all-site cancer profiles were estimated. The secular trend of different histological incidences and the proportion changes were analyzed. Results The incidence of female thyroid cancer was 6. 55/100000 in 2006, ranking the eighth in the female cancer profile. The incidence of thyroid cancer showed an increased secular trend in recent 26 years, especially a 5.7-fold increase in the papillary thyroid cancer (PTC). The incidence for female increased by 267% in 2006 compared to that in 1981, APC% was 3. 1%. The variation ranked second among all the female tumors. The incidences of thyroid cancer in Shanghai and the United States also elevated with time.Conclusions Thyroid cancer has been one of female common tumors from a once less common tumor,especially the proportion of PTC is expanding. It suggests that further study on the risk factors and preventive efforts should be made.

The purpose of this study is to explore depression metabolic markers in rat hippocampus and to investigate the anti-depressant effect of genipin and its mechanisms using nuclear magnetic resonance (NMR) metabonomics. Chronic unpredictable mild stress (CUMS) procedure was conducted to establish the depressive rat model. At the beginning of the third week, genipin low dose (25 mg x kg(-1)), middle dose (50 mg x kg(-1)), high dose (100 mg x kg(-1)), and venlafaxine (50 mg x kg(-1)) were given to the CUMS rats separately once daily for two weeks except control and model groups. Rat hippocampus was analyzed by 1H NMR based metabonomics after drug administration for 2 weeks. Significant differences in the metabolic profile of rat hippocampus of the CUMS treated group and the control group were observed with metabolic effects of CUMS including decreasing in glycine and N-acetylaspartate, increasing in inositol, glutamate, lactate, glutamine, taurine and alanine. Genipin showed ideal antidepressive effects at a dose of 50 mg x kg(-1) in rats, decrease of inositol, glutamate, lactate, alanine were observed, while glycine and N-acetylaspartate were increased. Important influence has been found on normal nervous system function of these significant changed metabolites, which suggests that the antidepressant effect of genipin may be played by enhancing the activity of neurons in hippocampus, repairing and improving the function of the neuron. The metabonomics approach is an effective tool for the investigation of the anti-depressant effect and pharmacologic mechanisms of genipin.