Objective·To investigate the role of collagen triple helix repeat containing-1 (CTHRC1) in ovarian cancer cell metastasis and the related mechanism. Methods·The expression of CTHRC1 in ovarian cancer cells was detected by Western blotting. The cell line which had high expression of CTHRC1 was transfected with a CTHRC1 specific shRNA, and the lenti-CTHRC1 was used to overexpress CTHRC1 in the cell line whose expression of CTHRC1 was very low. Then the expression of Slug and MMP-2 was assessed. Transwell assay was used to determine the migration and invasion capability of ovarian cancer cells after transfection. Results·The expression of CTHRC1 in HO8910 cells was the lowest, while the CTHRC1 protein level was dramatically increased after transfection of lenti-CTHRC1. Meanwhile, there was a distinct rise of the migration and invasion ability, as well as the expression of Slug and MMP-2 (all P<0.05). Conversely, CaOV3 cells had a higher protein expression of CTHRC1. By using lenti-shCTHRC1, a remarkable knockdown of CTHRC1 was obtained. Likewise, the capability of migration and invasion was decreased, and the Slug and MMP-2 expression was reduced (all P<0.05). Conclusion·CTHRC1 might positively regulate Slug and MMP-2 to promote ovarian cancer cell metastasis.

Objective To clarify the clinical and imaging features of the small centrum ovale infarcts by comparing with the small basal ganglia infarcts. Methods Forty-six consecutive patients with small centrum ovale infarct showed on the axial MR diffusion weighted imaging admitted to hospital within one week after onset were enrolled retrospectively. One hundred fifty-seven patients with small basal ganglia infarct were used as a control group. The differences of demography,vascular risk factors,clinical features, and imaging data in patients with small centrum ovale infarct and small basal ganglia infarct were compared and analyzed. Results The mean age of small centrum ovale infarct was 69 ± 12 years,among them, 27 patients were male. The mean age of small basal ganglia infarct was 66 ± 11 years,among them,98 were male. The vascular risk factors,clinical features and imaging data of the small centrum ovale infarct and the small basal ganglia infarct were compared. There were significant differences in hypertension (63. 0% [n =29]vs. 43. 3% [n = 68],P = 0. 018),coronary heart disease (4. 3% [n = 2]vs. 17. 8% [n = 28],P =0. 042),atrial fibrillation (15. 2% [n = 7]vs. 5. 7% [n = 9],P = 0. 036),single limb weakness (17. 4% [n = 8]vs. 6. 4% [n = 10],P = 0. 021),National Institutes of Health Stroke Scale score (2 [1,3]vs. 3 [2,5],P = 0. 002),infarct diameter (6 ± 3 mm vs. 10 ± 3 mm,P < 0. 01),ipsilateral middle cerebral artery(MCA)stenosis (4. 3% [n =2]vs. 24. 2% [n =38],P = 0. 006),and accompanied with contralateral intracranial artery stenosis (ICAS)(4. 3% [n = 2]vs. 17. 8% [n = 28 ],P = 0. 042). Conclusions Compared with the small basal ganglia infarcts,the prevalence of atrial fibrillation of the small centrum ovale infarcts was higher. The degree of neurological deficits on admission was milder,the diameter of the infarct was smaller,and the incidences of ipsilateral MCA stenosis and contralateral ICAS were lower.

Objective Toinvestigatetherelationshipbetweendifferenttypesofinternalwatershed infarctionandtandemstenosesofinternalcarotidartery(ICA).Methods Atotalof55patientswith internal watershed infarction confirmed by head MRI and diffusion-weighted imaging (DWI )examination were enrolled. They all underwent the extracranial internal carotid artery (ICA ) ultrasonography and intracranial cerebral artery MR angiography (MRA)examinations. According to the findings of imaging,the 55 patients with internal watershed infarction were divided into a simple internal watershed infarction (IWSI)group and an internal watershed infarction accompanied with ipsilateral cortical watershed infarction (C-IWSI)group. The relationship between the two types of internal watershed infarction and tandem stenoses of ICA was analyzed. Results (1 ) Of the 55 patients with internal watershed infarction,24 cases (43. 6%)were in the internal watershed infarction group and 31 cases (56. 4%)were in the C-IWSI group. The ipsilateral vascular stenosis were ICA 20 cases (36. 4%,including extracranial segment 11 cases and intracranial segment 17 cases),middle cerebral artery (MCA)44 cases (80. 0%), and tandem stenoses of ICA 15 cases (27. 3%). (2)Ipsilateral tandem stenoses of ICA:2 cases were in the IWSI group (intracranial ICA+MCA 2 cases);13 cases were in the C-IWSI group (extracranial ICA+intracranial ICA +MCA 4 cases,extracranial ICA + intracranial ICA 1 case,extracranial ICA + MCA 2 cases,and intracranial ICA+MCA 6 cases). (3)Compared with the IWSI group,the incidences of ipsilateral ICA stenosis and tandem stenoses of ICA in patients of the C-IWSI group were higher (54. 8%[n=17]vs. 12. 5%[n=3],41. 9%[n=13]vs. 8. 3%[n =2]),and there were significant differences(P =0. 001, 0.006]). The incidences of extracranial and intercranial ICA stenosis were higher than those of the IWSI group (35. 5%[n=11]vs. 0,45. 2%[n=14]vs. 12. 5%[n=3]),and there were significant differences (P=0.003,0.009).Conclusion Inthedifferenttypesofinternalwatershedinfarction,theincidenceof tandem stenoses of ICA is different. The IWSI patients with ipsilateral cortical watershed infarction often accompany by tandem stenoses of ICA.

Objective To observe the effect of bandage contact lens on cornea epithelial healing after Epi-LASIK,and to evaluate the efficacy and safety.Methods Prospective case-control study.The 92 cases(181 eyes) performing Epi-LASIK were devided into observation group and control group.The 46 cases(91 eyes) of the control group were covered with routine nursing and the other 46 cases (90 eyes) of the observation group were served with bandage contact lens after Epi-LASIK.The symptoms and signs,time of corneal epithelial recovery,visual acuity and pain of postoperation of the two groups were examined within one week.Results After Epi-LASIK treatment,there was no significant difference in visual acuity between the two groups(P ＞ 0.05).At the 1 st,3rd,and 7th day after Epi-LASIK treatment,the ache grading results of the observation group were (2.53 ± 0.62),(1.39 ± 0.71),(0.07-± 0.25);and the ache grading results of control group were (3.22 ± 0.79),(1.74 ± 0.49),(0.26 ± 0.49).Pain scores of observation group were significantly lighter than that of the control group (P ＜ 0.05).After Epi-LASIK treatment,the average time of corneal wound healing in the observation group and control group were (2.80 ±0.86) days and (4.07 ±0.68) days respectively.The average time of postoperative corneal wound healing in the observation group was shorter than control group(P ＜ 0.05).Conclusion The application of bandage contact lens on cornea epithelial healing after Epi-LASIK treatment can reduce the pain of patients and promote the recovery of cornea in myopia patients.

Objective: Through the bibliometrics analysis, to understand the research status quo and trend of the application of gamification in health management. Methods: Based on the Web of Science Core Collection, We retrieved the article, review and proceedings paper published from 1900 to 2018 and analyzed literature retrieved in term of document types, publication years, authors, and funding agencies. In addition, VOSviewer1.6.9 was used for visualization analysis of keywords and countries/regions. Results: A total of 364 pieces of literature were retrieved, of which 177 (48.63%) pieces were article. Since 2013, the number of literature has shown an upward trend at a rapid speed. However, only 16.48% of the literature were published by high productivity authors, which means a core group of authors has not yet formed in this research direction. The United States published the most literature, 89 pieces. Seven of the top ten funding agencies by volume were belong to the United Kingdom. However, only 10 pieces of literature were published by China. A total of 55 high-frequency keywords were included in this study, forming 5 research topics: physical activities management of adolescent based on mobile devices; internet-based behavior and mental health management for older-adults; design and application of fitness games; intervention and promotion of youth health behavior; the application of mobile health in adult self-management. The new high frequency keywords included older-adults, adults, mental health etc. Conclusion The application of gamification in health management is in its infancy, but it develops rapidly. European and American countries are in a leading position, while China is relatively backward. The research topics tend to be diversified and gamification is initially applied to the older-adults, adults and mental health management. In the future, the application scope of gamification in health management should be further expanded and effectively combined gamification with mHealth.

Objective To investigate the clinical and radiographic features of rheumatoid arthritis (RA) patients with cervical subluxations.Methods The clinical, laboratory and imaging data of 68 RA patients who were hospitalized to our hospital because of cervical syndrome were retrospectively reviewed.The patients were divided into two groups depend based on whether cervical spine subluxations presented or not.Mann-Whitney U test, t test and Person correlation, x2 test were used for statistical analysis.Results This study confirmed the high frequency (11.6％, 39/335) of radiological cervical subluxations in RA patients.The nNeck pain was a common symptom, accounting for 87％ (34/39).Other symptoms included limb numbness 59％ (23/39).The rate of positive C-reactive protein (CRP) in the subluxation group was both significantly higher than that of the non-subluxation group [89％(24/27) vs 59％(16/27),x2=6.17, P＜0.01].Anterior atlantoaxial subluxation (AAS) was the most common form, accounting for 95％(37/39) of all cervical subluxations,followed by vertical subluxation (VS), accounting for 18％ (7/39).Subaxial subluxation (SAS) of the lower cervical vertebrae was less common (5.1％).The ratio of bone destruction, spinal stenosis, spinal cord compression in image findings of the subluxation group was significantly higher than that of the non-subluxation group (x2=7.96, x2=6.12, x2=4.89, P＜0.05).But more hyperostosis and osteosclerosis feature of the non-subluxation group could be observed more hyperostosis and osteosclerosis feature (x2=6.21, P＜0.05).PADI correlated with ADI (r=-0.588, P=0.015).Conclusion This study confirms the high frequency of radiological cervical involvement in patients with RA.AAS is the most common form of cervical involvement and may occur either independently or concomitantly with cranial settling and subaxial subluxation.

Polysaccharides are important large molecules in the body. It has many functions such as activting the lymphocytes like T,B, NK and M? cells, promoting the secretion of cytokines and antibodies through binding to the CR 3 receptor of lymphocytes, affecting the concentrations of cellar [Ca 2+ ] i, cAMP, cGMP, NO and PGE 2, and promoting the gene expression of cytokines. Its function also relate to its molecule weight and structure.

Objective To investigate the effect of electroacupuncture (EA) pretreatment on neurological function, and the expression of Toll-like receptor 4 (TLR4) and nuclear factorκB (NF-κB) protein in ischemic penumbra after cerebral ischemia-reperfusion (CIR) injury. Methods A total of 36 male Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12) and EA group (n=12). The later two groups were occluded their right middle cerebral arteries for two hours and reperfused. The EA group was pretreated with EA at Baihui (GV20) for two weeks before modeling. They were assessed with modified Neurological Severity Scores (mNSS), the injury in ischemic brain was detected with HE staining, and the expression of TLR4 and NF-κB protein in ischemic penumbra were detected with Western blotting, 24 hours after reperfusion. Results The score of mNSS decreased (P<0.05), the injury of brain tissue ameliorated, and the expression of TLR4 and NF-κB decreased in ischemic penumbra (P<0.05) in EA group compared with those in the model group. Conclu-sion EA pretreatment can reduce the injury and improve the neurological function in rats after CIR by down-regulating the expression of TLR4 and NF-κB protein in ischemic penumbra.

Objective To study the relationship between single subcortical small infarction (SSSI) in middle cerebral artery (MCA) and large artery atheromatous sclerosis (LAAS).Methods One hundred and sixty patients with acute SSSI in MCA admitted to our hospital from January 2014 to December 2015 were divided into proximal infarction group (n=98) and distal infarction group (n=62).Their demographic,clinical,imaging data and National Institutes of Health Stroke Scale (NIHSS) score were recorded on admision.Results The incidence of coronary heart disease,NIHSS score,and contralateral intracranial artery stenosis were significantly higher while the incidence of proximal infarction,old lacunar infarction,and periventricular leukoaraiosis was significantly lower in proximal infarction group than in distal infarction group (21.4％ vs 3.2％,P=0.001;3 vs 2,P=0.030;19.4％ vs 1.6％,P=0.002;10.2％ vs 29.0％,P=0.002;16.3％ vs 33.9％,P=0.010).Logistic regression analysis showed that proximal infarction was independently related with old lacunar infarction,contralateral intracranial artery stenosis and periventricular leukoaraiosis (OR=0.270,95％CI:0.100-0.726,P=0.009;OR=4.500,95％CI:1.541-15.012,P=0.018;OR=0.325,95％CI:0.127-0.834,P=0.019).Conclusion Distal SSSI in MCA is related with small vessel disease while proximal SSSI in MCA is related with LAAS.

Taenia solium is one of the most important human parasites and its cysticercus and adult worms are both pathogenic.Cysticercosis caused by cysticercus is the most serious.In recent years,a large number of studies have been conducted on the immunological pathogenesis at cellular and molecular levels in local and abroad.The relation between cysticercus invasiveness and host immune effects and immunological pathogenesis has been preliminarily clarified.This article reviews the immunological pathogenesis of cysticercosis to provide new ideas for the immunoprevention and treatment of this disease.