Objective To explore the morphology changes of spinal cord after nursing intervention on motor function repair spinal cord injuried rats. Methods 60 adult SD rats were randomly divided into normal control group, experimental control group, and experimental group ( n=20 for each group) . Each group were divided into four time phase points, that is 1 day, 7 days, 30 days and 60 days after spinal cord injury (n=5). The model of L4 plane with full transection of spinal cord were made in the rats in experimental control group and exper-imental group. Normal control group were of untreated normal rats, experimental control group were given routine nursing such as urination and defecation after spinal cord injury, and experimental group were given passive movement practices and muscle massage training twice a day (10 min each time) besides regular nursing. HE staining and immunohistochemistry method were applied to observe the morphology changes of spinal cord. Results In experimental control group and experimental group there was no significant changes in HE staning and NF-200 and GFAP immunohistchemistry staning in spinal cord section of rat at each time phase points, but compared to the normal control group, it was of significant difference. Conclusion There is no apparent change in morphology in injured spinal area after nursing intervention.

Objective To explore the effect of nursing intervention on degree of motor function recovery after spinal cord injury in rats. Methods 60 rats were randomly divided into three groups with 20 rats in each group. Group A was the control group,group B was the rou-tine nursing group,and group C was the nursing intervention group which was given nursing intervention such as muscle pressure,joint passive movement,and skin care. The ethology was evaluated by BBB score and inclined-plane score at different time points after spinal cord injury. Myoelectricity and wet weight of gastrocnemius muscle were observed at different time points after spinal cord injury through multi-channel physiological signal acquisition system and electronic weighing scales. Results BBB score and inclined-plane score were basically the same. Compared with group B,hind limb function of group C was significantly improved,but it was obviously lower than the control group. Amplitude of gastrocnemius myoelectrical fibrillation in group C was higher than that in group B and gastrocnemius muscle wet weight decrease in group C was lower than that in group B. Conclusion Nursing intervention can delay the speed of gastrocnemius muscle atrophy,improve motor function,and promote the recovery of partial function after the spinal cord injury in rats.

OBJECTIVE:To probe into the cost control in PIVAS of our hospital in order to provide reference for the effective cost control.METHODS:The status quo of cost control of PIVAS was analyzed to provide corresponding countermeasures.RESULTS & CONCLUSIONS:The effective cost control can be achieved through improving cost accounting,controlling cost and reducing running cost and labour cost to promote the healthy development of PIVAS.

Objective To observe the effect of bandage contact lens on cornea epithelial healing after Epi-LASIK,and to evaluate the efficacy and safety.Methods Prospective case-control study.The 92 cases(181 eyes) performing Epi-LASIK were devided into observation group and control group.The 46 cases(91 eyes) of the control group were covered with routine nursing and the other 46 cases (90 eyes) of the observation group were served with bandage contact lens after Epi-LASIK.The symptoms and signs,time of corneal epithelial recovery,visual acuity and pain of postoperation of the two groups were examined within one week.Results After Epi-LASIK treatment,there was no significant difference in visual acuity between the two groups(P ＞ 0.05).At the 1 st,3rd,and 7th day after Epi-LASIK treatment,the ache grading results of the observation group were (2.53 ± 0.62),(1.39 ± 0.71),(0.07-± 0.25);and the ache grading results of control group were (3.22 ± 0.79),(1.74 ± 0.49),(0.26 ± 0.49).Pain scores of observation group were significantly lighter than that of the control group (P ＜ 0.05).After Epi-LASIK treatment,the average time of corneal wound healing in the observation group and control group were (2.80 ±0.86) days and (4.07 ±0.68) days respectively.The average time of postoperative corneal wound healing in the observation group was shorter than control group(P ＜ 0.05).Conclusion The application of bandage contact lens on cornea epithelial healing after Epi-LASIK treatment can reduce the pain of patients and promote the recovery of cornea in myopia patients.

Background: Feline panleukopenia virus (FPV) is a widespread and highly infectious pathogen in cats with a high mortality rate. Although Yanji has a developed cat breeding industry, the variation of FPV locally is still unclear. Objectives: This study aimed to isolate and investigate the epidemiology of FPV in Yanji between 2021 and 2022. Methods: A strain of FPV was isolated from F81 cells. Cats suspected of FPV infection (n = 80) between 2021 and 2022 from Yanji were enrolled in this study. The capsid protein 2 (VP2) of FPV was amplified. It was cloned into the pMD-19T vector and transformed into a competent Escherichia coli strain. The positive colonies were analyzed via VP2 Sanger sequencing. A phylogenetic analysis based on a VP2 coding sequence was performed to identify the genetic relationships between the strains. Results: An FPV strain named YBYJ-1 was successfully isolated. The virus diameter was approximately 20–24 nm, 50% tissue culture infectious dose = 1 × 10 −4.94 /mL, which caused cytopathic effect in F81 cells. The epidemiological survey from 2021 to 2022 showed that 27 of the 80 samples were FPV-positive. Additionally, three strains positive for CPV-2c were unexpectedly found. Phylogenetic analysis showed that most of the 27 FPV strains belonged to the same group, and no mutations were found in the critical amino acids. Conclusions A local FPV strain named YBYJ-1 was successfully isolated. There was no critical mutation in FPV in Yanji, but some cases with CPV-2c infected cats were identified.