Method of improved pp biuret with high sensitivity and accuracy is frequently used in the biochemical techniques to determine the protein in the preparations. The sensitivity of this method was as same as this of method Anson and error of pp biuret was smaller than this of Anson. The pp biuret has been applied in Vietnam to determine the proteolytic activity of pharmaceutical pepsin.
Biuret Reaction ; Pepsin A

This study was undertaken to know whether the UV-irradiation of the skin causes changes in the superoxide dismutase(SOD) activities. After shaving, the back skin of the rabbit was irradiated with UV light ranging from 280 to 320 nm of wavelengths from Burdick lamb (UV800) in doses of either 0.5, 1.0 or l.5 J/cm2. The skin was removed imrnediately after irradiation and the enzyme activity was assayed by the method of McCord ad Fridovich (xanthine-xanthie oxidase system), One unit of the SOD activity was defined as the amount of the enzyme required to inhibit the rate of reduction of cytochrome c by 50%. The protein content of the enzyme was determined by biuret method. The SOD activity of the skin irradiated with 1.0 J/cm was 7. 78 + 1.62 unit/mg protein(Mean+SD; n=10), significantly higher than that of the control(nonirradiated) group(5.62+1.57 unit/mg protein; n=l0). No significant changes were found in the skins irradiated with 1.5 and 1.5 J/cm. This finding indicates that UV irradiation is capablc of increasing the SOD activitiea in the rabbit skin, and suggests that increased superoxide formed by UV irradiatiun induces the increased SOD activies.
Biuret ; Cytochromes c ; Oxidoreductases ; Skin* ; Superoxide Dismutase* ; Superoxides* ; Ultraviolet Rays

OBJECTIVETo investigate the biuret reagent to detect proteins in the application, the impact of different test conditions for test results.

METHODSThe biuret method to select three different instruments, reagents, calibrators are arranged in combination to form 27 sets of detection systems, each detection system is a combination of 5 serum samples for testing, 5 measured values obtained, the selection process normality good a serum for the study to determine the mean value of all AST after culling outliers obtained in order to calculate the various detection systems use a combination of biuret reagent to detect proteins bias.

RESULTSThe use of different detection equipment to detect proteins biuret reagent bias, homogeneity of variance (P = 0.467), the difference was not statistically significant (F = 1.688, P = 0.421). different detection reagents using biuret reagent to detect proteins bias, homogeneity of variance (P = 0.574), a statistically significant difference (F = 5.784, P = 0.011). different calibrators use biuret reagent to detect proteins bias, homogeneity of variance (P = 0.467), the difference was statistically significant (F = 5.289, P = 0.000).

CONCLUSIONBiuret reagent in the detection of protein applications, impact detection reagents and calibrators will test result, during the test than when it is necessary to detect deviation detection reagents and calibrators due to be considered.

Biuret ; chemistry ; Blood Proteins ; analysis ; Calibration ; Indicators and Reagents ; chemistry

With the availability of the method of analysis of serum protein using minute amounts of material, it was felt desirable to understand the protein metabolism and physiologic function in the body. The present study was undertaken to clarify the serum albumin, globulin and total protein at term to demonstrate the normal concentration and correlation between the 30 mother and newborn infant pairs. Serum albumin, globulin and total protein were determined by the Biuret method with pooled human serum. The A/G ration was calculated by formula of A/G. The following result were obstained. 1) In comparing the newborn infants of nonanemic mothers a albumin and total protein concentrations were higher and globlin concentrations decreased in the anemic mothers. 2) In comparing the nonanemic mothers and anemic mothers the mean albumin concentrations were nearly equal but globulin and total protein were slightly increased in the nonanemic mothers. 3) The mean serum albumin(of maternal and umbilical cord blood) was 3. 8+/-0. 35 gm/100 ml and 3. 8+/-0. 49 gm/100 ml respectively. 4) The mean serum globulin of mate. nal and umbilical cord blood was 2. 7+/-0. 41 gm/100 ml and 2. 32+/-0. 47 gm/100 ml respectively. The correlation of the globulin status between mot-hers and their newborn infants was not significant(r=0. 32). 5) The m-an serum total protein of maternal and umbilical cord blood was 6. 59+/-0. 59 gm/100 ml and 6. 02+/-0.57gm/100ml respectively.
Biuret ; Fetal Blood ; Humans ; Infant, Newborn* ; Metabolism ; Mothers* ; Serum Albumin* ; Umbilical Cord

This study was performed to examine the characteristics of protein of red crab (Chionoecetes japonicus) shell powder hydrolyzed by commercial proteases. Red crab shell was digested by commercial proteases, such as Protamex (P), Neutrase (N), Flavourzyme (F), Alcalase (A), Protease M (PM) and Protease A (PA). Protein yield analyzed by Biuret assay, absorbance at 280 nm and brix revealed that PA was the enzyme having the highest proteolytic activity. SDS PAGE showed that molecular weight of proteins produced by protease treatments was various and below 150 kDa. Combinational treatment of proteases (PA + P, PA + PM, PA + F, PA + A) was tried whether these increase protein hydrolysis from red crab shell powder compared to a PA single treatment. Soluble protein content was similar, but amino acid concentration by combinational treatments was higher than PA single treatment [PA + P 247.4 mg/g > PA + F (206.4 mg/g) > PA + A (133.4 mg/g) > PA + PM (59.1 mg/g) > PA (54.9 mg/g)]. Amino acid composition by combinational treatments was slightly different. Most abundant essential amino acids were phenylalanine, glycine, alanine, and leucine, whereas tyrosine and cystine were not detected.
Alanine ; Amino Acids, Essential ; Biuret ; Cystine ; Electrophoresis, Polyacrylamide Gel ; Endopeptidases ; Glycine ; Hydrolysis ; Leucine ; Metalloendopeptidases ; Molecular Weight ; Peptide Hydrolases ; Phenylalanine ; Proteins ; Subtilisins ; Tyrosine

Extensive knowlege of the characteristics of synovial fluid has been available for at least the past 30 years, when a Monograph on the subject by Kling first appeared in 1938. Since that time, Ropes, Bauer(1953) and Hollander (1960, 1961, 1965) have published classic. Monographs on their extensive studies and findings of synovial fluid. Specific laboratory tests for diagnosis of various forms of arthritis are usually lacking. For example, the test for the rheumatoid factor in serum may be helpful in establishing the diagnosis of rheumatoid arthritis, but these are often negative in early cases and L. E. phenomenon is often negative in the early stage or between severe exacerbations of the Systemic lupus erythematosus. It has become increasingly clear during the past 10 years that synovial fluid analysis is both the most valuable and yet the most neglected differential diagnostic test for arthritis. Studies of synovial fluid have presented a virtually unexplored frontier in the investigation of arthritis. So, we studied the synovial fluid from 100 cases of various forms of arthritis in the Department of Orthopedic Surgery, Severance Hospital from May, 1968 to May, 1969. 100 cases of arthritis are; 30 cases of Osteoarthritis, 20 cases of Traumatic athritis, 25 cases of Rheumatoid arthritis, 10 cases of Septic arthritis, 5 cases of Tuberculous arthritis, and 10 cases of Non-specific bursitis. The synovial fluid were aspirated from the involved joints in aseptic conditions and follwing studies were done. 1) General appearance. 2) Mucin content by Acetic acid PPT. or Ropes test. 3) Viscosity by Drop test. 4) Cell count by Wright s stain. WBC: Total and differential count. RBC count. 5) Synovial sugar by Folin Wu method. 6) Fasting blood sugar by Folin Wu method. 7) Sugar difference between synovial sugar and Fasting blood sugar. 8) Total protein by Kingsley s Biuret method. 9) Bacterial culture in Septic arthritis. 10) Microscopic examination. RA cells by Sternheimer-Malbin stain in Rheumatoid arthritis. Cartilage fragments with simple wet preparations in Osteoarthritis. 6 kinds of arthritides were grouped into 3 categories based on the degree of inflammation of the synovial membrane as reflected by synovial fluid changes according to Ropes and Bauer s classification(1953). The first group, consisting of Osteoarthritis and Traumatic arthritis, was associated with mild inflammatory reactions and increased amount of fluid, but no significant changes in the number of WBC, sugar concentration, or quality of mucin. The second group was characterized by more sever inflammation of the synovial membrane and included Rheumatoid arthritis, Septic arthritis and Tuberculous arthritis. The second group was associated with decreased mucin content, increased WBC, polymorphonuclear leucocytes, RBC and protein and decreased amount of synovial sugar. RA cells were found in all cases of Rheumatoid arthritis and cartilage fragments in Osteoarthritis under the light microscope. The third group, an intermediate group-Non specific bursitis might have some distinguishing characteristics of synovial fluid but these were not usually diagnostic.
Acetic Acid ; Arthritis ; Arthritis, Infectious ; Arthritis, Rheumatoid ; Biuret ; Blood Glucose ; Bursitis ; Cartilage ; Cell Count ; Diagnosis ; Diagnostic Tests, Routine ; Fasting ; Inflammation ; Joints ; Lupus Erythematosus, Systemic ; Methods ; Mucins ; Netherlands ; Orthopedics ; Osteoarthritis ; Rheumatoid Factor ; Synovial Fluid ; Synovial Membrane ; Viscosity