Objective To understand the blood ABO,Rh blood group distribution of the main nationalities in Baoshan city to set up the local rare blood group donors archives for meeting the voluntary blood donor recruitment and the need of clinical blood use. Methods The ABO,Rh were detected by the microplate method.124 RhD negative individuals were extracted and screened for C/c,E/e by the test tube method.Results Among 7 main ethnic groups with blood donor,the ABO blood group distribution of Han, Yi,Bai,Lisu and Hui nationalities were A>O>B>AB;which in Brown and Dai nationalities were O>B>A>AB;the Rh negative ratio was 0.33% in Han,0.35% in Yi,0.09% in Bai,0.00% in Brown,0.32% in Lisu,0.21% in Dai,0.18% Hui;the Rh pheno-types were mainly ccdee (60.5%),Ccdee (33.1 %),CCdee (4.0%)and ccdEe (2.4%).Conclusion The distribution rate of ABO blood group in Han,Yi,Bai,Lisu,Hui blood donors in Baoshan was consistent with the distribution of Han population in China;Brown and Dai are different from Han in China;the Rh negative frequency is 0.00%-0.35%,which reflects the regional and na-tional characteristics of blood group distribution in baoshan city.