This study was conducted to investigate of water qualities in the upper and middle reaches of the Han River. For this purpose, water was sampled at Kwangjin and 1st Han-River Bridges of the Han River in Seoul and analysed from August, 1988 to September, 1989. The results are summarized as follows: 1. Water quality at 1st Han-River Bridge was more polluted than that at Kwangjin Bridge. 2. Except biological oxygen demand (BOD), turbidity, suspended solid (SS), dissolved oxygen (DO), DO saturation (DOS), ammonia nitrogen (NH3-N), nitrite nitrogen (NO2-N) and chloride ion (Cl-) at Kwangjin and 1st Han-River Bridges were lower as compared with the previous data before redevelopment of the Han River. 3. SS, DO and pH at Kwangjin and 1st Han-River Bridges could be classified to the 1st grade in environmental water quality standard. DOS at Kwangjin Bridge was over 100% and that at 1st Han-River Bridge was below 100% in the Han River. BOD at Kwangjin Bridge could be classified to 2nd grade and that at 1st Han-River Bridge to 3rd grade in environmental water quality standard. 4. The higher the level of water was, the lower the levels of turbidity and SS, and NH3-N was decreased with increasing water level at 1st Han-River Bridge. DO was decreased as water temperature went up but DOS was increased with DO. BOD was positively correlated with nitrite-nitrogens. 5. Turbidity and SS at the both sites and Chloride ion (Cl-) at Kwangjin Bridge were increased in July and August. And DO at the both sites and NH3-N at 1st Han-River Bridge were decreased in at July and August.
Ammonia ; Biological Oxygen Demand Analysis ; Hydrogen-Ion Concentration ; Nitrogen ; Oxygen ; Rivers ; Seasons* ; Seoul ; Water Quality ; Water*

Early-warning of compartmentalized anaerobic reactor (CAR) was investigated in lab-scale. The performance stability of CAR at high loading rate was worse than that at common loading rate. At high loading rate, the fluctuation of effluent chemical oxygen demand (COD) concentration and volatile fatty acids (VFA) concentration was larger than that of influent COD concentration. The average relative standard deviation of effluent COD concentration and VFA concentration was 32.95% and 40.46% respectively, while that of influent COD concentration was 8.08%. The saturation of volumetric loading rate (S(VLR)) and VFA (S(VFA)) could be used to alarm the performance of anaerobic reactors. The working performance was good when the CAR was operated at normal organic loading rate (OLR), in which S(VLR) and S(VFA) were below 0.89 and 0.40 respectively. The fluctuation of performance became larger when the CAR was operated at OLR near saturation, in which S(VLR) and S(VFA) were close to 1. The performance of CAR was deteriorated when the S(VLR) and S(VFA) were more than 1.
Anaerobiosis ; Biological Oxygen Demand Analysis ; Bioreactors ; microbiology ; Fatty Acids, Volatile ; analysis ; Sewage ; Waste Disposal, Fluid ; methods

In order to simultaneously remove carbon and nitrogen from organic-rich wastewater, we used an up-flow anaerobic sludge bed/blanket (UASB) reactor that was started up with anammox with high concentration of carbon and nitrogen by gradually raising the organic loading of influent. We optimized the removal of nitrogen and carbon when the chemical oxygen demand (COD) concentration varied from 172 to 620 mg/L. During the entire experiment, the ammonium and total nitrogen removal efficiency was higher than 85%, while the average COD removal efficiency was 56.6%. The high concentration of organic matter did not restrain the activity of anammox bacteria. Based on polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and tapping sequencing analyses, the Planctomycete, Proteobacteria, Chloroflexi, Chlorobi bacteria are detected in the UASB reactor, which indicated complex removal pathway of carbon and nitrogen coexisted in the reactor. However, a part of Planctomycete which referred to anammox bacteria could tolerate a high content of organic carbon, and it provided help for high performance of nitrogen removal in UASB reactor.
Ammonia ; chemistry ; Biological Oxygen Demand Analysis ; Bioreactors ; Carbon ; chemistry ; Nitrogen ; chemistry ; Sewage ; Waste Disposal, Fluid ; methods ; Waste Water ; chemistry

Regulation of alkaline-resistant laccase from Perenniporia tephropora KU-Alk4 was proved to be controlled by several factors. One important factor was the initial pH, which drove the fungus to produce different kinds of ligninolytic enzymes. P. tephropora KU-Alk4 could grow at pH 4.5, 7.0, and 8.0. The fungus produced laccase and MnP at pH 7.0, but only laccase at pH 8.0. The specific activity of laccase in the pH 8.0 culture was higher than that in the pH 7.0 culture. At pH 8.0, glucose was the best carbon source for laccase production but growth was better with lactose. Low concentrations of glucose at 0.1% to 1.0% enhanced laccase production, while concentrations over 1% gave contradictory results. Veratryl alcohol induced the production of laccase. A trace concentration of copper ions was required for laccase production. Biomass increased with an increasing rate of aeration of shaking flasks from 100 to 140 rpm; however, shaking at over 120 rpm decreased laccase quantity. Highest amount of laccase produced by KU-Alk4, 360 U/mL, was at pH 8.0 with 1% glucose and 0.2 mM copper sulfate, unshaken for the first 3 days, followed by addition of 0.85 mM veratryl alcohol and shaking at 120 rpm. The crude enzyme was significantly stable in alkaline pH 8.0~10.0 for 24 hr. After treating the pulp mill effluent with the KU-Alk4 system for 3 days, pH decreased from 9.6 to 6.8, with reduction of color and chemical oxygen demand at 83.2% and 81%, respectively. Laccase was detectable during the biotreatment process.
Biological Oxygen Demand Analysis ; Biomass ; Carbon ; Copper ; Copper Sulfate ; Fungi ; Glucose ; Hydrogen-Ion Concentration ; Ions ; Laccase* ; Lactose ; Physiology