At the blood transfusion and heamatology centre of Ho Chi Minh city, we performed phenotyping for 158 cases of acute leukemia diagnosed from 04/98 to 10/1998. Basing on these results, we go further to monitor residual disease depending on the presence of aberant antigens and DNA content. The enumeration of progenitor cell CD34+ also performed for leukemia patient during the bone marrow transplantation
Hematologic Diseases ; Biological Markers

A study on the effects of longterm hepamarin therapy on some hematological and biochemical indicators in 50 experimental male mice was carried out. The hepamirin was a preparation from phyllantus amarus used at dose of 5 g/kg of bodyweight within 3 months. The results have shown that above mentioned dose of hepamarin did not affect on the number of leukocyte, erythrocyte and body weight of mice as well as liver and kidney functions
Biological Markers ; Animals ; Phyllanthus

The study was carried out in six stored blood by CPD-A1, at the first, 4th, 14th, 21st, 28th, 35th and 42nd days of the blood stored. The results showed that: The plasma glucose and pH; 2.3-DPG concentration were decreased, plasma lactat was increased gradually during blood stored by time, the difference was significantly than that in the first point of time with p<0.001. The plasma G-6-PD and PK activities were significantly decreased during blood stored by time.
Biological Markers ; Blood ; Mutation

The extraction, the activity and the content of hormons (testosteron, estrogen and progesteron) from some Echinodermata at Viet Nam were reported. The subjet: Echinodermata collected at Thanh Hoa and Nha Trang. The result: The solven system of methylen chloroid and of methanol have higher effect than of ethanol and ether ethylic. The extracts from egg of sea star, sea urchin tested on white rat and rabbit were proved to have activity of testosteron, estrogen and progesteron. Egg of sea star, sea urchin, pyloric ceaca of sea star and meat of Bohadschia argus Jaeger have adequately steroid hormones (testosteron, estrogen and progesteron). In pyloric ceaca of see star, the content of testosteron, estrogen and progesteron are higher than in their egg
Biological Markers ; Hormones ; Echinodermata

Primary mixed germ cell tumor is a very rare tumor in the cranial cavity. It is composed of varying combination of germ cell neoplasms, such as germinoma, embryonal carcinoma, choriocarcinoma and teratoma. The authors experienced a case of mixed germ cell tumor developing in the floor of the third ventricle, in a twelve years old boy. He was admitted to the hospital, because of headache, eyeball pain, diplopia and vomiting. Brain CAT scan revealed a round tumor density in the floor of the third ventricle. Tumor marker study, preoperatively checked, revealed beta-HCG, 439.8 mIU/ml and alpha-fetoprotein, under 20 ng/ml. On operation, there was a dark brown firm mass up to 3.0 cm in the third ventricle, that invaded into the foramen Monro. With microscopic examination, this tumor is composed chiefly of germinoma, with embryonal carcinomatous region, choriocarcinomatous region and focal mature teratomatous region. The authors diagnosed this case as mixed germ cell tumor.
Tumor Markers, Biological

Biological behavior of malignant tumors has been assessed by morphological grading, clinical staging, and estimating other tumor markers. Recently DNA ploidy measured by flow cytometry and image analyser has been suggested as an additional useful indicator of the tumor behavior. In order to extract useful tumor cell-specific information in ovarian mucinous tumors, DNA contents and other morphologic parameters were measured by image analysis and DNA ploidy was also measured by flow cytometry. In all cases of cystadenoma, DNA diploidies were observed. In borderline malignancy, DNA diploidies were chiefly observed except one case of polyploidy. In true malignancy, DNA aneuploidies were observed except one case of polyploidy and two cases of diploidies by image analysis, and except four cases of diploides and one cas of polyploidy by flow cytometry. The statistical significance were observed in DNA ploidy pattern by image analysis. In nuclear areas, perimeters and major axis, statistical significance were not observed. These results suggest that DNA ploidy pattern are more or less independent parameter as an additional useful indicator of the histological grade of malignancy and that image analysis are better than flow cytometry in detecting DNA aneuploidy.
Tumor Markers, Biological

Immunohistochemical staining on paraffin sections for S-100 protein improved diagnostic accuracy for melanocytic tumor. But specificity of S-100 protein in the diagnosis of melanocytic tumor is very low, because S-100 protein was also expressed in neurogenic tumor and salivary gland tumor. To investigate a specific tumor marker for the malignant melanoma, immunohistochemical staining for HMB-45 and S-100 protein was performed on the paraffin sections of 25 cases of malignant melanoma and 46 cases of nevi. Positive reaction for HMB-45 and S-100 protein was diffusely identified in the cytoplasm of tumor cells. Positive ratio for HMB-45 was 100% in malignant melanoma, 92% in junctional component of compound nevus and 0% in intradermal nevus. Positive ratio for S-100 protein was 92% in malignant melanoma, 100% in compound nevus and 100% in intradermal nevus. The sensitivity and specificity for HMB-45 in malignant melanoma were 100%, but those for S-100 protein were 92% in sensitivity and 86.7% in specificity. These results indicate that HMB-45 has a high sensitivity and specificity for malignant melanoma cells and it can be quite useful for the histopathological diagnosis of malignant melanoma.
Sensitivity and Specificity ; Tumor Markers, Biological

Epidermal growth factor receptor (EGFR) is an intergral membrane protein. Overexpression or mutation of EGFR may play a role in careinogenesis. Recently, many molecular biologic techniques have been used to study expression of oncogenes. One of them, in situ mRNA hybridization, using paraffin embedded blocks, offers a unique means to allow precise localization within histological preparations, and also overcomes problems relating to translation defects and abnormal translation. In order to confirm the usefulness of epidermal growth factor receptor as a tumor marker, and to compare the expression of EGFR between in situ MRNA hybridization and an immunohistochemical study, in situ MRNA hybridization was performed along with an immunohistochemical study for EGFR in paraffin sections of 84 uterine cervix carcinomas. A positive reaction for EGFR was observed mairdy in the cytoplasm of tumor cells. The vascular muscle layer and uterine muscle tissue around the cancer nest revealed a positive reaction in immunohistochemical stain for EGFR, with a negative reaction for EGFR mRNA. In the cancer nests, the immunohistochemical positive reaction for EGFR was strong in differentiated cells and keratin pearls, but a strong positive reaction for EGFR mRNA was localized in undifferentiated cells. The overall positive of immunostaing for EGFR was 77% for uterine cervix carcinoma; 71 % for carcinoma in situ, 71 % for microinvaseve carcinoma, and 89% for invasive carcinoma. The overall positivity of EGFR from in situ MRNA hybridization was 94% of the uterine cervix carcinoma; 93% for carcinoma in situ, 93% for microinvasive carcinoma, and 96% for invasive carcinoma. From these results, EGFR is a useful tumor marker for uterine cervix carcinoma, and in situ mRNA hybridization has greater sensitivity and specificity than immunohistochemistry.
Sensitivity and Specificity ; Tumor Markers, Biological

Generality - Material - Method: The IMX PSA, CA 19-9, CEA are Microparticle Enzyme Immune Assay (MEIA) for the quantitative measurement of tumor marker in human serum. Results: number of health subjects 30 (20 men, 10 women), number of patients 56 (42 men, 14 women). The results of studies indicated that the PSA assay value is frequently elevated > 10ng/ml in the serum of subjects with prostatic cancer, the sensitivity 70.6%. CA 19-9 assay value is elevated > 37 U/ml in the serum of pancreatic cancer subjects, the sensitivity (83.4%). CEA assay value is elevated > 10 ng/ml in the serum of colorectal carcinoma, the sensitivity (54.5%).
Prostatic Neoplasms ; Tumor Markers, Biological

The quantitative analysis of the concentration tumor marker CA15-3 by the immunoradioactive method in the serum of 26 normal persons without the tumor, hepatic diseases and chronic diseases ages of 18-50 has shown that this is method with high accuracy. The radioactive activity is direct proportional with the concentration of CA 15-3; The concentration of CA 15-3 in the normal people is 19,12+/-15,54 u/ml in which 90% persons have concentration under 30% u/ml. The concentration of CA 15-3 is most common in 4,71 u/ml.
Tumor Markers, Biological ; Chronic Disease