Primary mixed germ cell tumor is a very rare tumor in the cranial cavity. It is composed of varying combination of germ cell neoplasms, such as germinoma, embryonal carcinoma, choriocarcinoma and teratoma. The authors experienced a case of mixed germ cell tumor developing in the floor of the third ventricle, in a twelve years old boy. He was admitted to the hospital, because of headache, eyeball pain, diplopia and vomiting. Brain CAT scan revealed a round tumor density in the floor of the third ventricle. Tumor marker study, preoperatively checked, revealed beta-HCG, 439.8 mIU/ml and alpha-fetoprotein, under 20 ng/ml. On operation, there was a dark brown firm mass up to 3.0 cm in the third ventricle, that invaded into the foramen Monro. With microscopic examination, this tumor is composed chiefly of germinoma, with embryonal carcinomatous region, choriocarcinomatous region and focal mature teratomatous region. The authors diagnosed this case as mixed germ cell tumor.
Tumor Markers, Biological

The study was carried out in six stored blood by CPD-A1, at the first, 4th, 14th, 21st, 28th, 35th and 42nd days of the blood stored. The results showed that: The plasma glucose and pH; 2.3-DPG concentration were decreased, plasma lactat was increased gradually during blood stored by time, the difference was significantly than that in the first point of time with p<0.001. The plasma G-6-PD and PK activities were significantly decreased during blood stored by time.
Biological Markers ; Blood ; Mutation

The extraction, the activity and the content of hormons (testosteron, estrogen and progesteron) from some Echinodermata at Viet Nam were reported. The subjet: Echinodermata collected at Thanh Hoa and Nha Trang. The result: The solven system of methylen chloroid and of methanol have higher effect than of ethanol and ether ethylic. The extracts from egg of sea star, sea urchin tested on white rat and rabbit were proved to have activity of testosteron, estrogen and progesteron. Egg of sea star, sea urchin, pyloric ceaca of sea star and meat of Bohadschia argus Jaeger have adequately steroid hormones (testosteron, estrogen and progesteron). In pyloric ceaca of see star, the content of testosteron, estrogen and progesteron are higher than in their egg
Biological Markers ; Hormones ; Echinodermata

At the blood transfusion and heamatology centre of Ho Chi Minh city, we performed phenotyping for 158 cases of acute leukemia diagnosed from 04/98 to 10/1998. Basing on these results, we go further to monitor residual disease depending on the presence of aberant antigens and DNA content. The enumeration of progenitor cell CD34+ also performed for leukemia patient during the bone marrow transplantation
Hematologic Diseases ; Biological Markers

A study on the effects of longterm hepamarin therapy on some hematological and biochemical indicators in 50 experimental male mice was carried out. The hepamirin was a preparation from phyllantus amarus used at dose of 5 g/kg of bodyweight within 3 months. The results have shown that above mentioned dose of hepamarin did not affect on the number of leukocyte, erythrocyte and body weight of mice as well as liver and kidney functions
Biological Markers ; Animals ; Phyllanthus

Biological behavior of malignant tumors has been assessed by morphological grading, clinical staging, and estimating other tumor markers. Recently DNA ploidy measured by flow cytometry and image analyser has been suggested as an additional useful indicator of the tumor behavior. In order to extract useful tumor cell-specific information in ovarian mucinous tumors, DNA contents and other morphologic parameters were measured by image analysis and DNA ploidy was also measured by flow cytometry. In all cases of cystadenoma, DNA diploidies were observed. In borderline malignancy, DNA diploidies were chiefly observed except one case of polyploidy. In true malignancy, DNA aneuploidies were observed except one case of polyploidy and two cases of diploidies by image analysis, and except four cases of diploides and one cas of polyploidy by flow cytometry. The statistical significance were observed in DNA ploidy pattern by image analysis. In nuclear areas, perimeters and major axis, statistical significance were not observed. These results suggest that DNA ploidy pattern are more or less independent parameter as an additional useful indicator of the histological grade of malignancy and that image analysis are better than flow cytometry in detecting DNA aneuploidy.
Tumor Markers, Biological

The oncogenes, which have been detected in various human solid tumors, transform culture cells, and the level of m-RNA specific for an oncogene increases in the cellular extract of the tumor cells. These findings suggested that oncogene expression was closely related with carcinogenesis. Recently, oncogen products were considered as tumor markers, but it was not confirmed that the relationship between quantitative change of oncogene product and malignant potential of a neoplasm. To evaluate the relationship between the quantitative change of oncogene product and malignant potential, immunohistochemical staining for the ras and myc oncogene products was performed in the sections of papilloma and transitional cell carcinoma of urinary bladder. 1) Positive reaction of c-ras oncogene product was noted along the cell membrane and in the cytoplasm, and c-myc oncogene product in the nucleus, and along the unclear membrane and cell membrane. 2) Tissue expression of c-ras oncogene was homogeneous and strong in the transitional cell carcinoma rather than in papilloma. 3) The ratio of the positive cells with c-ras oncogene product was 35.1% in the papilloma, 79.4% in the grade I, 81.9% in the grade II, 87.6%, in the grade III of transitional cell carcinoma of the urinary bladder. There was a tendency for the ratio to increase with the degree of histological grading. 4) By the immunoperoxidase staining of c-myc oncogene product, the number of the cells showing positive nuclear staining incrased with the tumor grading.
Humans ; Tumor Markers, Biological ; Carcinogens

In March 2001, on white mice, herba pipers lolot collected in Ha Noi surburd was studied concerning the chemical components, its toxicity and antiinflamative pain relief activities. Results showed a same chemical composion in the root and the stem with alcaloid, volatile oil, flavonoids, anthranoids, tannin, reductive sugar, amimo- acid…Total flavonoid level accounted for 1,14%. On thin layer chromatography there were 8 stains of flavonoid, 5 of alcaloids. About toxicity: decoction fluid of the dose of 200g/kg body weight, no toxicity was found. In alcoholic 90o extract with the dose of 20 g/kg body weight there was an antiinflamnative effect just at the 1st hour to 30th hour of the experiment, 39,63 - 69,15% of inflammative manfestation were inhibited. The solution of 4% total flamvonoid inhibited inflammation from 41,08% to 58,21%. Pain relief: in both methods alcoholic extact inhibited 62,50%- 64,05% and flavonoid in 4% solution inhibited 42,6- 54,6% equivalent to aspirine
Chemistry ; Biological Markers ; Medicine, Herbal

The study on antioxydative activities of the polarizied of BoBo on order to expoit its pharmaco-biological effect efficiently. Results showed that antioxydative activity at cell membrane lipid invitro and invivo of 14 extracted products of Bo Bo leaf Bts, Bn, B1-B12 was evaluated Dn invitro, experiment dose of 0,1 mg/ml of Bts, B1,B2, B3, B4,B5 manifested significant antioxydant activities, among then Bts and B5 have the highest activity, their preparations have the highest inhibition quality
Antioxidants ; Plants, medicinal ; Biological Markers

Chemical investigation of the leaves of Gelsemium elegans growing in Vietnam resulted in the isolation of the indol alkaloids: gelsevirine, gelsenicine and koumine. The isolated compounds from this plant were tested for the antimicrobial and cytotoxicity activity
Indole Alkaloids ; Biological Markers ; Alkaloids