With more than 20 years of development, Pichia pastoris system has been extensively used both on a lab and industrial scale. This review outlines the progress made on P. pastoris from aspects of protein expression, molecular engineering tools and methods, and biochemical production. This review also provides perspectives on the current challenges and future directions of this important system.
Bioengineering ; Industrial Microbiology ; Pichia

Engineering three-dimensional (3D) implantable tissue constructs is a promising strategy for replacing damaged or diseased tissues and organs with functional replacements. However, the efficient vascularization of new 3D organs is a major scientific and technical challenge since large tissue constructs or organs require a constant blood supply to survive in vivo. Current approaches to solving this problem generally fall into the following three major categories: (a) cell-based, (b) angiogenic factor-based, and (c) scaffold-based. In this review, we summarize state-of-the-art technologies that are used to develop complex, stable, and functional vasculature for engineered 3D tissue constructs and organs; additionally, we have suggested directions for future research.
Bioengineering ; Tissue Scaffolds

Strengthening practical teaching, together with improving innovation ability is one of the key tasks of Emerging Engineering Education. This paper is based on the revision of the training program of bioengineering in School of Chemical Engineering and Technology, Tianjin University, improved the practical teaching system and curriculum content, built a five-level teaching system for basic experiment, comprehensive experiment, course design, scientific research and practical training. In order to cultivate outstanding innovative talents with practical ability and innovative spirit, innovative teaching reform mode is proposed. Furthermore the new thought and new schemes for Emerging Engineering Education are put forward.
Bioengineering ; education ; Curriculum

Biochemical Reaction Engineering is a professional discipline with emphasis on both theory and practice. It is the core course in the curriculum provision for students majoring in bioengineering and plays an important role in the cultivation of professional talents. However, there are so much theory knowledge, such as formulas and deduction, that students showed poor efficiency in their study. Herein, to cater the objective command of innovative talents under new education background, and to improve the practical teaching efficiency and the students' enthusiasm as well as the practical innovation capability, the teaching team innovated and explored the teaching pattern, the teaching method as well as the teaching technologies. The teaching efficiency has been remarkably improved by introducing the virtual simulate technology, the micro-lecture, the case teaching pattern attempts and the scientific platform, which can be used as reference by other peers.
Bioengineering ; Curriculum ; Humans ; Students

In a systemvalidation of a ambulatory EKG analysis system we examined the HP 43420A ambulatory EKG analysis system(Fa.Hewlett packard, USA). The complete capture module 43405A with the applied software is able to record 24 hours events without single beat lable. Model 43400B patient analyzer records intermittant the EKG events and represents these in the Ambulatory EKG report. By means of the devices 31 patients were examined. Beat-to-beat analysis of the complete full disclosure is visually carried out. Sensitivity and postitve predictive value were 94(96)% and 100% for ventricular ectopic beat(VEB). The sensitivity amounted to 87% in the detecting of VEB pair(couplet), the positive predictive value 94%. VEB Run/VT(ventriular tachycardia) resulted in a sensitivity of 82% and in positive predictive value of 93%. The automatically edited Ambulatory EKG report with his EKG strips is representative of full disclosure and reliable in comparison with the analyzed results of full disclosure. The HP 43420A Ambulatory EKG Analysis system, a new and developed device with all digital recording system and full disclosure capability is found comparable to the best ones of the presently available Holter monitoring system.
Bioengineering* ; Disclosure ; Electrocardiography ; Electrocardiography, Ambulatory* ; Humans

A knife-edge method is used here to measure the beam width of the highly repetitive high-power thin disk Yb:KYW femtosecond laser (MABEL-Mannheim Biomedical Engineering Lab, University of Heidelberg, Germany). Presented in this paper is the detailed measuring process together with the results verified by theoretical calculating and scanning electron microscope measurements respectively. Therefore, it is concluded that the knife-dege method is an effective beam width measurement tool of high-power femtosecond lasers.
Bioengineering ; instrumentation ; Lasers ; Microscopy, Electron, Scanning ; instrumentation

In the "Tutorial for outline of the healthy China 2030 plan", biomedicine was listed as a key planning and development area. Shanghai government also lists biomedicine as an emerging pillar industry. The rapid development of biomedicine industry put higher requirement for talents. Taking the idea of cross integration, mutually beneficial development, inheritance and innovation, the School of Biotechnology of East China University of Science and Technology organically integrates bioengineering and pharmaceutical majors to develop a new undergraduate engineering program of biomedicine, which specially reforms the talent training practice from the aspects of developing a "trinity teaching" standard system, a "three integration, three convergence" curriculum system, and a "three comprehensive education" innovative talent training system. We put forward the trinity of "value guidance, knowledge system, technology and non-technical core competence literacy" to foster emerging biomedicine engineering talents, and developed a comprehensive innovative talents training mode featured by "covering class-in and class-out, covering every student, and covering ideology and curriculum". Moreover, we established effective connections between courses and training goals, between general education courses and professional courses, and between top-notch talent training systems and training programs. Based on the achievements of teaching reform of the emerging engineering program "intelligent bio-manufacturing", the experience we obtained may provide ideas for development of the first-class bioengineering major in China.
Bioengineering ; Biomedical Engineering ; China ; Curriculum ; Humans ; Students

"Bio-separation engineering" is a compulsory course for undergraduate students majored in bioengineering, and an important part of the "emerging engineering education" system for bioengineering. Our teaching team follows the principle of "student development as the center, innovation thinking as the core". Guided by the concept of "learning achievement", we reconstructed the teaching contents of this course, and carried out the teaching reform aiming at solving several long-standing problems. These include, for instance, the theoretical teaching is separated from the experimental practice, and students cannot internalize the theoretical knowledge into practical ability in time. Moreover, the contents of course is out-of-date and out of line with industry demand, the teaching form and assessment methods are relatively single, and the students' professional ability and quality are not effectively cultivated. In the new curriculum system, in which the "online" and "offline" teaching are both applied, we broke the boundary between theoretical and experimental courses, and made the contents keep up with the forefront of industry development through research-based teaching. In terms of teaching methods and teaching evaluation, we made full use of modern information technology to enrich classroom teaching activities, and carried out complete, dynamic and diversified assessment for students. These teaching reform measures greatly improved the students' interest in learning this course, as well as their professionalism and research ability.
Bioengineering ; Biomedical Engineering ; Curriculum ; Humans ; Learning ; Students

According to the teaching philosophy of the outcome-based education, this study elaborates the development of a practical innovation course for biological engineering major after five runs of teaching practice and continuous improvement. It mainly includes the methods for selection of teaching subjects, implementation of teaching process, process assessment, evaluation and improvement. Based on the performance and achievements of three grades of students majored in bioengineering, we found that the logic and methods of the practical innovation course could greatly stimulate the motivation of students for learning, as well as their scores. Therefore, the logic and methods described in this study may serve as a reference for the reforms of practical training courses of engineering major under the background of Engineering Education Certification.
Bioengineering ; Certification ; Curriculum ; Humans ; Learning ; Students

"Biochemical Engineering Experiment" is a compulsory curriculum for the concentrated practical teaching of biotechnology majors in Hunan University of Science and Engineering. It is also an experimental curriculum for improving the overall quality of bioengineering students under the context of "Emerging Engineering Education". The course includes comprehensive experiments and designable experiments, and the contents of which are designed by combining the local characteristic resources of Yongzhou, the research platform and the characteristics of the talents with engineering background. In the teaching practice, methods such as heuristic teaching, research cases-embedded teaching and interactive teaching are comprehensively used to boost students' interest in learning and stimulate their innovative thinking and application capability. Through curriculum examination and post-class investigation, it was found that the students' abilities of knowledge transfer and application were significantly improved, and they achieved excellent performances in discipline competitions and approved project proposals. The practice and continuous improvement of this course may facilitate fostering high-level innovative and application-oriented talents of biotechnology majors.
Humans ; Curriculum ; Students ; Learning ; Bioengineering ; Biomedical Engineering