BACKGROUND: Conventional implantation at anterior aesthetic region is difficult to achieve ideal three-dimensional position, and needs high implantation accuracy. OBJECTIVE: To evaluate the clinical application effect of three-dimensional printed digital guide technology in anterior dental implant. METHODS: Eighty-six cases of dental implants were divided into two groups. Control group (n=43, 52 implants) received conventional dental implantation. Trial group (n=43, 52 implants) received implantation under three-dimensional printed digital guides. The accuracy of the three-dimensional position of the implants was measured. The modified plaque index, modified bleeding index, probing depth and the satisfaction of patients with implant denture were measured at 6 months after implantation. RESULTS AND CONCLUSION: (1) The errors of vertical and horizontal directions at the top and the root after implantation in the trial group were significantly fewer than those in the control group (P < 0.05). (2) The modified plaque index, modified bleeding index, and probing depth in the trial group were significantly lower than those in the control group (P < 0.05). (3) The satisfaction of patients in the trial group was 98% (42/43), which was higher than 86% (37/43) in the control group. (4) In summary, three-dimensional printed digital guide plate used in dental implant dentures can improve the three-dimensional position accuracy after implantation and improve the postoperative clinical effect.

BACKGROUND: Studies have shown that conventional flap-guided bone tissue regeneration is a method commonly used in the immediate implantation of the upper anterior teeth. It is an ideal method for repairing small bone defects, but it is difficult to achieve favorable osteogenesis effect in the tooth neck area. OBJECTIVE: To evaluate the clinical effects of modified flap immediate implant in maxillary aesthetic area. METHODS: Sixty cases of immediate implant placement were investigated, and randomized into two groups: 30 received modified flap immediate implant in which a gingiva former with suitable size was screwed followed by buried healing (experiment group) and 30 received traditional flap immediate implant in which a cover screw was screwed followed by buried healing (control group). They were impressed digitally and restored with all-ceramic crown 6 months after the surgery. Implant retention rate, pink esthetic scores, gingival growth and labial bone absorption were detected and compared between two groups. RESULTS AND CONCLUSION: The implant retention rates of both groups were 100%. Compared with the control group, the pink esthetic score and gingival growth were significantly increased in the experimental group (P < 0.05), and the labial bone thickness was significantly decreased (P < 0.05). The proximal, distal or mesial gingiva growth was increased a bit in the experimental group compared with the control group, but there was no significant difference between the two groups (P > 0.05). To conclude, the modified flap immediate implant is conducive to soft tissue reconstruction in maxillary aesthetic area.

Objective: To understand the status of blood-borne occupational exposure and protection among health care workers (HCWs) in dental practice, and provide evidence for making effective prevention. Methods: From April 1 to 15, 2019, a stratified random sampling method was conducted to investigate the data of blood-borne occupational exposure among 221 dental HCWs in 2018, and Chi-squared Test was used to evaluate the differences of status on occupational exposure and protection among different professionals, such as nurses, doctors and trainees, and among different-grade hospitals. Results: A total of 166 HCWs were exposed to occupational exposure 269 times, with the annual incidence of 75.11% (166/221) , and 37.55% (101/269) of exposures were reported. However, all source patients of exposures had failed to be traced. 89.59% (241/269) of exposures were sharp injuries. The top three instruments caused injuries were syringe/bilan needles, suture needles and vehicle needles, accounting for 35.68% (86/241) 、16.60% (40/241) and 16.18% (39/241) , respectively; and the top three exposure operations were removal/disposal of needles or instruments, suture/assisting suture and injection of anesthetic, accounting for 37.17% (100/269) 、22.30% (60/269) and 17.84% (48/269) , respectively. There were statistically significant differences among different professionals in occupational exposure frequency, reporting rate, the types of instruments caused injuries, exposure operations and hepatitis B vaccinated time (P<0.01) . The compliance rate of standard precautions, safe operation, post-exposure reporting and prevention, and training on occupational protection were generally poor among HCWs, with significant differences in different-grade hospitals (P<0.01) . Conclusion There is a high incidence, low reporting rate and poor self-protection of blood-borne occupational exposure among dental HCWs. Strongly suggesting that standard precautions, safe use and disposal of oral instruments, active post-exposure report and prevention must be improved for everyone.

【Objective】 To investigate the relationship between cerebrospinal fluid exosome-derived miR-630 and the function of brain microvascular endothelial cells （BMECs）. 【Methods】 The subarachnoid hemorrhage endothelial cell model was established to evaluate the effect of hemorrhagic cerebrospinal fluid （BCSF） on BMECs’ proliferation by MTT assay and cell cycle analysis. qRT-PCR and immunofluorescence staining were used to detect the expressions of endothelial cell tight junction protein （ZO-1） and adhesion molecule （ICAM-1 and VCAM-1）. Changes in NOx concentration were detected by radioimmunoassay. The cerebrospinal fluid exosomes in the experimental group （co-incubated with BCSF） and the control group （normal cerebrospinal fluid） were isolated and identified， and differences in the expressions of cerebrospinal fluid exosomal miR-630 between the two groups were compared. BMECs work changes after the intervention with miR-630 analogue were observed. 【Results】 The proliferation of BMECs was significantly inhibited in the experimental group； the mRNA and protein levels of ICAM-1， VCAM-1 and ZO-1 were significantly decreased， and the function of endothelial cells was significantly inhibited （P<0.05）. After the successful separation and identification of cerebrospinal fluid exosomes， the expression of miR-630 was significantly lower in the experimental group than in the control group （P<0.05）. The function of BMECs was significantly improved with miR-630 mimics. 【Conclusion】 The low expression of miR-630 in cerebrospinal fluid exosomes after subarachnoid hemorrhage is closely related to BMECs injury.

As a highly prevalent global condition, myopia significantly impacts the ocular health of young individuals in China. Orthokeratology lens, as a rigid corneal contact lens, has demonstrated effective control over the progression of myopia; however, its mechanism of action remains incompletely elucidated. As one of the factors influencing visual acuity, higher-order aberrations will undergo marked changes after orthokeratology, with particular emphasis on the alterations in spherical aberrations and coma. The changes in corneal morphology induced by orthokeratology lead to significant positive increase in both spherical aberration and coma. Furthermore, the elevation of spherical aberration and coma demonstrates a negative correlation with the rate of axial length growth following orthokeratology. The interplay among spherical aberration, coma, defocus, accommodation, astigmatism, and pseudo-accommodation may constitute the underlying mechanism governing the control of myopia through orthokeratology.

OBJECTIVE: To evaluate the technical feasibility,safety,and clinical outcome of mechanical thrombectomy with Solitaire FR stent system for embolic occlusion of the superior mesenteric artery(SMA).METHODS: The clinical data of 6 patients with embolic occlusion of the SMA treated by mechanical thrombectomy with Solitaire FR stent system between January 2015 and June 2018 in Binzhou City People's Hospital were analyzed retrospectively.RESULTS: Superior mesenteric artery occlusion was initially diagnosed by computed tomography(CT)in all patients.A successful thrombus removal of superior mesenteric arterial by Solitaire FR stent system was observed in the 6 patients.Five patients had recovered well after operation and no complications such as artery dissection,perforation and hemorrhage or intestinal ischemia.One patient underwent bowel resection.CONCLUSION: The arterial mechanical thrombectomy with solitaire FR stent system are characterized with high rate of recanalization,fine security,minimal invasion and less complications in patients with acute superior mesentericvarterial embolism.

Cataract is one of the major causes of vision loss and even blindness in patients, and surgery is the only effective method to treat it. The pathogenesis and precaution of cataract remain hot issues in ophthalmological research. With the maturation of biotechnology in recent years, modeling methods and species of experimental animals have become more diverse, which are still the mainstay of cataract mechanism research. However, the ideal animal model of cataract has yet to be constructed due to the complexity of human cataract etiology. Herein, the modeling principles, in vivo or in vitro modeling methods, characteristics, and existing problems of animal models of cataract are summarized according to etiology, providing the theoretical foundation for the construction of a comprehensive animal model that more closely resembles the human cataract.

OBJECTIVETo study the hazards of demulsifier production process.

METHODSThe air of production environment was monitored, and physical examinations were conducted on 52 exposure workers and 53 non-exposure workers (control), all the medical checks including clinical, electromyography and laboratory checks.

RESULTSThe air concentration of propylene oxide was 4.6 mg/m3, and the concentration of Ethylene oxide was 4.8 mg/m3. There was significant difference between exposure group and control group in terms of the incidence of neurasthenic syndromes, lachrymation, laryngopharyngeal ache, inappetence, tired and quadriplegia (P <0.01). The incidence of Achilles's tendon reflection, knee jerk slacking up, chronic pharyngitis, coryza, acne, hair lose and pneumonia were significantly higher in study group than that in control group (P <0.01). Peripheral nerve damage of impairment incidence rate was 40.4%, significantly higher than that in control group (9.4%). No significant difference was found between groups on IgG, IgA and IgM. And also did the rate of chromosome aberrance (CA), sister chromatid exchange (SCE) and micronuclie cell (MC) of peripheral blood (P >0.05).

CONCLUSIONThe production environment of demulisier could stimulate respiratory channel and affect peripheral nerve and central nerve, but no aberrance effects were found.

Adult ; Case-Control Studies ; Epoxy Compounds ; toxicity ; Ethylene Oxide ; toxicity ; Female ; Humans ; Male ; Middle Aged ; Occupational Exposure ; Young Adult

Recombinant human endostatin is an antiangiogenic drug, which limits the progression of tumor through normalize the tumor vessels and inhibits the proliferation of tumor cells in combination with chemotherapeutic drugs; and directly inhibits the formation of neovascularization endothelium.Gastric cardia carcinoma is a common digestive tumor in China, which is mainly treated by surgical resection, radiotherapy and chemotherapy.Based on the principle of recombinant human vascular endostatin and its good effect in the treatment of various kinds of malignant tumors, especially in the radiochemotherapy of digestive tract malignant tumors.In order to inhibit the development of tumor, improve patients′ postoperative survival rate, and prolong the survival time of disease free progression, this article will discuss the application value of recombinant human vascular endostatin in the comprehensive therapy of cardiac carcinoma.

Vascular endothelial growth factor (VEGF) is a mult-effective catokines on the endothelial cells specificly. It promotes the endothelial cells to split multiply proliferate and metastasis. It increases vascular permeability and accelerates new vascular generation. VEGF participates many physiological and pathological processes. It has achieved more clinical application and will have extensive applicative prospect.
Animals ; Brain Ischemia/drug therapy* ; Cell Division/physiology* ; Cell Movement/physiology* ; Endothelium, Vascular/metabolism* ; Forensic Medicine/methods* ; Humans ; Myocardial Ischemia/drug therapy* ; Rats ; Vascular Endothelial Growth Factors/therapeutic use*