Objective To develop a rapid SERS detection method based on monolithic column for detection of dye adul-terated natural indigo .Methods The dyes in natural indigo were extracted and mixed with silver colloid .The spectra were re-corded after applying the mixture solution to the monolithic column since the intertwined pores in monolithic column could con-tribute for the distribution of silver nanoparticles .Results SERS signals of malachite green dyed natural indigo at quantity as low as 500 μg/kg could be obtained .Conclusion This simple ,fast and specific SERS detection method based on monolithic col-umn could be used for rapid detection of stained natural indigo .

Objective: To determine the most influential data features and to develop machine learning approaches that best predict hospital readmissions among patients with diabetes. Methods: In this retrospective cohort study, we surveyed patient statistics and performed feature analysis to identify the most influential data features associated with readmissions. Classification of all-cause, 30-day readmission outcomes were modeled using logistic regression, artificial neural network, and EasyEnsemble. F1 statistic, sensitivity, and positive predictive value were used to evaluate the model performance. Results: We identified 14 most influential data features (4 numeric features and 10 categorical features) and evaluated 3 machine learning models with numerous sampling methods (oversampling, undersampling, and hybrid techniques). The deep learning model offered no improvement over traditional models (logistic regression and EasyEnsemble) for predicting readmission, whereas the other two algorithms led to much smaller differences between the training and testing datasets. Conclusions: Machine learning approaches to record electronic health data offer a promising method for improving readmission prediction in patients with diabetes. But more work is needed to construct datasets with more clinical variables beyond the standard risk factors and to fine-tune and optimize machine learning models.