Objective To evaluate surgical indications and methods for regional pancreatoduodenectomy combined with blood vessel reconstruction. Method Eight patients underwent pancreatoduodenectomy combined with superior mesenteric vein and portal vein (SMV/PV) resection and reconstruction from May 2001 to December 2004,respectively. Results The overall mortality was 0 during perioperative period,no complications occurred. Histological specimen examinations demonstrated adenocarcinoma of pancreas head in all patients. The resected endothelium or margins of the blood vessel and pancreas were microscopically tumor free in all cases. Patients were followed-up from six months to four years.Two patients were died within one year. Four patients had survived for more than two years. Conclusion Regional pancreatoduodenectomy combined with blood vessel reconstruction could increase tumor resection rate in properly selected patients, and could be performed safely without increased morbidity and mortality.

We developed a high-efficiency microfluidic chip for extracting exosomes from human plasma. We collected peripheral blood from normal human, designed and fabricated a microfluidic chip based on nanoporous membrane and agarose gel electrophoresis to isolate exosomes. The extracted exosomes were characterized by transmission electron microscopy, nanosight and Western blotting, the morphology, concentration and particle size of exosomes were identified and analyzed. Meanwhile, we used ultracentrifugation and microfluidic chip to isolate exosomes separately. The particle size and concentration of the exosomes extracted by two methods were compared and analyzed, and their respective extraction efficiency was discussed. Finally, the expression level of miRNA-21 in exosomes was analyzed by RT-PCR. The microfluidic chip isolated (in 1 hour) high-purity exosomes with size ranging from 30-200 nm directly from human plasma, allowing downstream exosomal miRNA analysis. By comparing with ultracentrifugation, the isolation yield of microfluidic chip was 3.80 times higher than ultracentrifugation when the volume of plasma sample less than 100 μL. The optimized parameters for exosome isolation by gel electrophoresis microfluidic chip were: voltage: 100 V; concentration of agarose gel: 1.0%; flow rate of injection pump: 0.1 mL/h. The gel electrophoresis microfluidic chips could rapidly and efficiently isolate the exosomes, showing great potential in the research of exosomes and cancer biomarkers.
Exosomes ; Humans ; MicroRNAs/genetics* ; Microfluidics ; Plasma ; Ultracentrifugation