In order to investigate the feasibility of the modified chitosan-gelatin crosslinked membrane (MC-Gel) and chitosan-gelatin crosslinked membrane (CS-Gel) to be a potential biomaterial for corneal regeneration, we evaluated their physicochemical properties and intraocular biocompatibility in this study. White light transmission and permeability of these membranes were detected. Results showed that white light transmission of both membranes was above 90% at 500 nm, which was similar to that of human cornea. The glucose, tryptophan and NaCl permeability of MC-Gel membrane and CS-Gel membrane was better than or similar to those of human cornea. The methylthiazol tetrazolium (MTT) assay was used to assess cell viability and proliferation. Also, interlamellar corneal transplantation was carried out to evaluate ophthalmic biocompatibility of MC-Gel membrane and CS-Gel membrane. Results indicated that MC-Gel membranes could support the proliferation of HCEC and displayed good intraocular biocompatibility when implanted into rabbits. No severe inflammatory reaction occurred after transplantation and the implanted MC-Gel membrane degraded completely 16 weeks post-operation. Due to its good physicochemical properties and biocompatibility, MC-Gel membrane could be a promising candidate material for corneal regeneration.
Animals ; Biocompatible Materials ; chemistry ; Cells, Cultured ; Chitosan ; chemistry ; Cornea ; cytology ; Corneal Injuries ; Cross-Linking Reagents ; Epithelium, Corneal ; cytology ; physiology ; surgery ; Gelatin ; chemistry ; Guided Tissue Regeneration ; methods ; Humans ; Membranes, Artificial ; Rabbits ; Regeneration ; Tissue Engineering ; methods ; Tissue Scaffolds

bjective? To expLore the mentaL response and nursing demands of spouses of puerpera in the puerperium and to provide support and assistance to famiLy-centered humanistic nursing in the department of obstetrics. Methods? TotaLLy 15 spouses of puerpera at day 3 post deLivery in a Class Ⅲ Grade A hospitaL in Suzhou were seLected by purposive sampLing from November 2017 to January 2018. Their reaL experience was coLLected by Live recording and semi-structured in-depth interview using quaLitative phenomenoLogicaL methods, and the data were organized, summarized and anaLyzed using content anaLysis. ResuLts? TotaLLy 4 categories were refined: adaptation to the roLe of husband, adaptation to the roLe of father, mentaL response and nursing demands. ConcLusions? The emotions of the spouses of puerpera in the puerperium fLuctuated significantLy, and their mentaL response vary from phase to phase. Attention shouLd be paid to the roLe awareness, responsibiLity, mentaL activity and nursing demands and information support and psychoLogicaL care shouLd be properLy provided according to their roLe awareness, responsibiLity and mentaL response.