A 76 year old male was admitted to hospital due to high- fever and pallor. Physical examination revealed fever, anemia, petechiane, yellowish sclera. Hemogram showed pancytopenia: WBC: 1.8 x 109/l (SN: 0.11; L 0.86; M 0.77x109/l); Hct: 26.4%; Hb: 8.7 g/dl; PLT: 63x109/l. Bone marrow aspiration and bone marrow biopsy showed hypocellularity (10-20%). Elevation of liver enzymes (SGOT/SGPT: 351/371 UI/I), hyperbilirubine (D/T: 1.8/2.1mg/dl), HbsAg (+). A diagnosis of aplastic anemia/hepatitis syndrome was made. The patient was given supportive treatment with blood transfusion, antibiotics, nutrition, polyvitamine. Two months later, the patient completely recovered from aplastic anemia and hepatitis.
Bone marrow ; Hepatitis B ; Case Reports [Publication Type]

Between 3/1990 and 5/2000, 249 patients with AML were treated at the Ho Chi Minh center for hematology and blood transfusion. The results were the following: complete remission achieved in 63% patients treated with 7&3 protocol, 77% with 7&3&5 protocol, 8% with Ara-C+ Purinethol protocol and 2% with Ara-C or Purinethol alone protocol. Overall survival at 12 months of patients treated with 7& 3, 7&3&5, and Ara-C+ Purinethol protocol were 32%, 69% and 16%, respectively. Conclusion, induction chemotherapy with 7&3 and 7&3&5 protocols achieved better results in patients with AML.
Leukemia, Myelocytic, Acute ; drug therapy

Objectives: The prevalence of posttraumatic stress disorder (PTSD) has increased, particularly among individuals who have recovered from coronavirus disease 2019 (COVID-19) infection. Health literacy is considered a “social vaccine” that helps people respond effectively to the pandemic. We aimed to investigate the association between long COVID-19 and PTSD, and to examine the modifying role of health literacy in this association. Methods: A cross-sectional study was conducted at 18 hospitals and health centers in Vietnamfrom December 2021 to October 2022. We recruited 4,463 individuals who had recovered from COVID-19 infection for at least 4 weeks. Participants provided information about their sociodemographics, clinical parameters, health-related behaviors, health literacy (usingthe 12-item short-form health literacy scale), long COVID-19 symptoms and PTSD (Impact Event Scale-Revised score of 33 or higher). Logistic regression models were used to examine associations and interactions. Results: Out of the study sample, 55.9% had long COVID-19 symptoms, and 49.6% had PTSD.Individuals with long COVID-19 symptoms had a higher likelihood of PTSD (odds ratio [OR], 1.86; 95% confidence interval [CI], 1.63–2.12; p < 0.001). Higher health literacy was associated with a lower likelihood of PTSD (OR, 0.98; 95% CI, 0.97–0.99; p = 0.001). Compared to those without long COVID-19 symptoms and the lowest health literacy score, those with long COVID-19 symptoms and a 1-point health literacy increment had a 3% lower likelihood of PTSD (OR, 0.97; 95% CI, 0.96–0.99; p = 0.001). Conclusion Health literacy was found to be a protective factor against PTSD and modified the negative impact of long COVID-19 symptoms on PTSD.

Aims: Microorganisms play a vital role in the breakdown of natural organic compounds and are valuable objects for worldwide enzyme production. The aim of this study was to identify favorable production conditions for Bacillus amyloliquefaciens D19 protease, followed by the purification and chemical characterization of this novel enzyme to assess its potential applications in various fields. Methodology and results: In this study, favorable conditions of protease production from B. amyloliquefaciens D19 were determined using a medium containing soluble starch (1.5%), earthworm extract (1.0%), yeast extract (0.5%), NaCl (1.0%), at pH 7.0-8.0, 37 °C for 36 h with 150 rpm shaking condition. The protease was purified and had a molecular weight of about 23 kDa. The optimum condition for casein hydrolysis was at 40 °C and pH 6.5-7.0 in the presence of 1.0 mM Na+ or 5.0 mM Zn2+. The enzymatic activity was maintained at 75-100% at 30-50 °C and in pH 6.0-10.0. The values of Vmax and KM were also determined as 1547 U/mg and 6.33 mg/mL, respectively. Conclusion, significance and impact of study The identified optimal conditions will serve as the foundation for the production of the 23 kDa B. amyloliquefaciens D19 protease, one of the smallest proteases within the Bacillus genus. Moreover, its notable heat resistance, broad pH tolerance, high substrate catalysis and moderate substrate binding affinity make this enzyme a promising candidate for various applications in the food-feed and brewing industries.