Objective:To evaluate the application effect of microlecture combined with PBL on the teaching of orthodontics.Methods:One hundred and forty undergraduates from Batch 2014 to Batch 2016 of School of Stomatology, Henan University were randomly divided into two groups, with 70 undergraduates in each group. Traditional teaching method was adopted in the control group, while microlecture combined with PBL was used in the observation group. The theoretical knowledge, clinical thinking and analysis ability, and operational skills were compared between the two groups. SPSS 18.0 was used to conduct t test and χ2 test. Results:The scores of basic knowledge [(83.67±5.73) points], case analysis [(82.64±5.54) points] and discussion and questions and answers [(81.57 ± 5.06) points] in the observation group were higher than those in the control group [(72.36±5.41) points, (70.41 ± 5.36) points, and (69.37±4.48) points, respectively], with statistically significant differences ( P < 0.05). The total excellent rate of clinical thinking and analysis ability and operational skills in the observation group were 94.29%(66/70) and 92.86%(65/70) respectively, which were higher than those in the control group [75.71%(53/70) and 74.29%(52/70)], with statistically significant differences ( P < 0.05). Conclusion:The application of microlecture combined with PBL has good effect on the teaching of orthodontics, which can improve the mastery of theoretical knowledge, clinical thinking and analysis ability and operational skills of students, and make them better apply theoretical knowledge in practical operation, thus improving comprehensive quality.