Dogs were bled and maintained at the mean arterial pressure(MAP)of 6.67 kPa(moderate shock)or of 5.33 kPa(severe shock)for 2 hours.Then the blood shed was transfused back to the animals and observation on the dogs was carried on for 2 more hours.26 mongrel dogs,weighing 10~16 kg,were randomized into Group I of normoxia and moderate shock(NMS,n=7),Group I of normoxia and severe shock(NSS,n=7).Group I of hypoxia at a simulated alti- tude of 4 000 m and moderate shock(HMS,n=6),and Group IV of hypoxia and severe shock(HSS,n=6).The changes of the cardiovascular function and the oxygen transport were observed in these animals after anesthetization.It was found that the cardiovascular function was similar in HMS and NMS groups.The decrease of VO2 of HMS animals was similar to that of NSS dogs.Decompensation occurred earlier in HSS animals and resulted in high mortality.

Objective To explore the personality type characteristic of children with cough variant asthma(CVA),so as to supply an individual treatment strategy.Methods With the aid of GONG Yao-xian revised Junior Eysenck Personality Questionnaire (JEPQ) by trained professionals,the personality of 65 cases of CVA children (CVA group) and 100 cases of common cold children (control group) was tested and evaluated.Then based on the dimension value P,E,N,L points of the curriculum,personality of orientation was evaluated.Results There was no significant difference in the P,N,L scores of JEPQ between CVA group and control group [(48.84 ± 10.26) scores vs.(48.15 ± 10.98) scores,(50.31 ± 10.49) scores vs.(49.25 ± 9.08) scores,(47.07 ± 9.31) scores vs.(47.20 ± 10.57) scores](P＞ 0.05).The E score of JEPQ in CVA group was lower than that in control group [(44.15 ± 9.29) scores vs.(50.25 ± 8.63) scores],and there was significant difference (P=0.000).Conclusions The CVA children have introverted character,and there is a tendency to form a personality type of conditional reflex.When medicine is taking,the individual personality type should be taken into consideration during the course of therapy.Appropriate mental hints may be helpful for medical therapy and make it a lot easier.

AIM: To investigate the possible role of prostaglandin, NO and potassium channel in the adaptive blunting of hypoxic pulmonary vasoconstriction (HPV) in the high altitude animal (pika). METHODS: The effect of L-NAME, indomethacin and 4-AP on the response of isolated lung strips of pika and Wistar rats instead of pulmonary artery to acute hypoxia were studied. RESULTS: (1) After inhibition of prostaglandins by indomethacin, the percentage increase in hypoxic constriction in lung tissue strip of pikas was greater than that in Wistar rats[(64.7?50.9)% vs (19.7?14.1)%], P

Objective To introduce the construction and application of clinical microbiology laboratory data management expert system.Methods Firstly, the process management was introduced to clinical microbiology laboratory. Then the characteristics of data on each node of work process were analyzed, and SQL Server data table was created as the knowledge base of the expert system.Finally, VB6.0 was used to compile the knowledge acquisition module, reasoning desktop module and input/output interface procedures to finally construct the expert system.Rates of defect report, errors in bacterial identification and drug sensitivity test, delay in culture results reporting and average delayed days were compared before and after the application of the expert system.Results The expert system could be used for data management in process nodes like sample reception, information collection and input, bacteria culture medium selection, bacterial identification and drug sensitive test, interpretation of drug sensitivity results, comprehensive evaluation in bacterial identification and drug sensitivity results, report of negative result, report of positive result, blood culture, Mycoplasma culture, time limit of detection, and nosocomial infection indicators.No defect report was found after the application of expert system; rate of errors in selection of drug sensitivity test medium was reduced from 0.81% ( 31/3 836 ) in 2012 to 0.02%(1/5 433) in 2014;rate of delay in culture results reporting was reduced from 1.78% (320/17 983) to 1.18%(232/19 692), and the average delayed days was also reduced (3.8 d vs.3.2 d).Conclusion Clinical microbiology laboratory data management expert system can improve work efficiency and reduce errors, which can enhance the overall management of laboratory and the quality of clinical service.

AIM and METHODS: Total RNA was extracted from 6th rat subcultured pulmonary artery smooth muscle cells(PASMC) exposed to continual chronic hypoxia or normoxia and the effects of chronic hypoxia on the changes of Kv1.3,Kv2.1,Kv3.1 mRNA in cultured PASMC induced by acute hypoxia were studied by semiquantitative RT-PCR in vitro. RESULTS:①Kv1.3,Kv2.1,Kv3.1 genes were found to be expressed in PASMC of rats exposed either to hypoxia or normxia.②The expression of Kv2.1 and Kv3.1 in 6th subcultured of PASMC in normaxia group could be upregulated by exposure to acute hypoxia,the levels of Kv2.1 and Kv3.1 mRNA were significantly increased from 0.646?0.092, 0.782?0.104 to 1.059?0.134, 0.985?0.116,respectively (P

OBJECTIVETo investigate whether transdermal scopolamine increased cardiac vagal activity in patients during the acute phase of myocardial infarction.

METHODS30 patients with a first acute myocardial infarction and preserved sinus rhythm who were on no drug that could influence the sinus node were randomly assigned to either treatment group or placebo group. Measures of heart rate variability (HRV) in patients given drug or placebo were obtained by digital 24 hour Holter recording before and after treatment. Baroreflex sensitivity was performed using the phenylephrine method.

RESULTSNo significant differences was found in the indices of the time domain and the frequency domain in both groups before treatment. Patients with transdermal scopolamine showed a significant increase in the standard deviation of normal RR intervals (SDNN), standard deviation of all five min mean normal RR intervals (SDANN), root mean square of differences of successive normal RR intervals (rMSSD), total power (TP, 0.000. - 0.40 Hz), low frequency peak (LF, 0.040 - 0.15 Hz), high frequency peak (HF, 0.15 - 0.40 Hz), and Baroreflex sensitivity after treatment (P < 0.05 - 0.01). These indices did not change in patients given placebo.

CONCLUSIONLow doses of transdermal scopolamine safely increase cardiac parasympathetic activity and improve autonomic indices in patients with acute myocardial infarction.

Administration, Cutaneous ; Adult ; Aged ; Baroreflex ; drug effects ; Dose-Response Relationship, Drug ; Female ; Heart ; innervation ; Heart Rate ; drug effects ; Humans ; Male ; Middle Aged ; Myocardial Infarction ; physiopathology ; Scopolamine Hydrobromide ; pharmacology ; Vagus Nerve ; drug effects ; physiopathology

Objective To investigate the clinical isolation situation and drug resistance features of enterococcal bacteria in order to provide reference for the clinical rational use of antibacterial agents and infection control. Methods A total of 1220 strains of enterococcal bacteria that induced hospital infection were analyzed retrospectively. Walk Away 96 automated microbial analyzer was used for strain identification and drug sensitive test. MIC was used for screening high-level aminoglycoside resistant strains. WHONET 5.6 was used for data analysis. Results A total of 1220 strains of enterococci were detected, including 675 strains of enterococci faecalis, accounting for 55.3%, and 445 strains of ente-rococci faecium, accounting for 36.5%. Enterococcal bacteria mainly distributed in clinical urine specimens, accounting for 57.5%. The total drug resistance rate of enterococci faecalis was high and the drug resistance rates to penicillin, ampicillin, ciprofloxacin and levofloxacin were all higher than 90%, which were significantly higher than those of the enterococci faecium (<17%). The drug resistance rate of enterococci faecalis to quinupristin/dalfopristin was 100.0%and that of enterococci faecium was 12.6%. For both types of bacteria, no strain resistant to vancomycin was found, but 3 strains of enterococci faecalis were resistant to linezolid. The screening rates of enterococci faecalis for high-level gentamicin drug resistant strains and high-level streptomycin resistant strains were 54.1% and 27.3% respectively while those of enterococci faecium were 58.2% and 56.9% respectively. Conclusion The drug resistance situation of enterococcal bacteria to common antibacterial drugs is not optimistic, and the monitoring of clinical distribution and drug resistance situation of enterococcal bacteria is of important guiding significance to the clinical treatment of entero-coccal bacterial infection.

Objective:To explore a modified flap bridging procedure in primary free flap transfer to reconstruct defects in calf and with secondary reconstruction of the great saphenous vein of the calf according to the diagnoses of 3 types of traumatic calf ulcers, and to observe the clinical effect.Methods:A research on evidence-based medicine was applied from April 2010 to June 2023 in the Department of Orthopaedics of the Second People's Hospital of Hefei on 25 patients with calf traumatic ulcers. The traumatic calf ulcers were classified into 3 types: Type I, a defect type (5 patients), Type II, a scar type (8 patients), and Type Ⅲ, a osteomyelitis type (12 patients). The age of patients ranged from 22 to 67 years old, with an average of 44 years old. The course of calf traumatic ulcers from 3 months to 36 years, with 17 in left calf and 8 in the right. The preoperative Lower Extremity Function Scale (LEFS) was 37.07 points±18.92 points. The modified flap bridge surgery was planned in 2 stages. The first stage surgery was simultaneously performed by 2 surgical teams, with surgical team 1 carried out ulcer debridement and preparation for a flap bridging surgery. According to the 3 types of diagnosis, the infected and unhealthy tissues were completely removed by individualised debridement, the sizes of defect were 5 cm×11 cm-14 cm×27 cm. Medial flaps were dissected to prepare a flap bridging (including great saphenous vein) with the proximal calf of the healthy side as the rotation point. Both lower legs were then fixed together. For an unhealed Type I or III fracture, the tibial fracture was reduced and fixed with an external fixator and then fixed with the contralateral tibia. For a healed Type II or III fracture, both calcaneus were fixed together with a Steinmann pin in obliquely penetration. The surgical team 2 carried out the task of harvest of a free flap. The flap with both adjustable design and anatomy was selected. The flap donor sites with a larger area and dominant vein were the best: anterolateral thigh flap (13 patients), thoracoumbilical flap (9 patients) and lateral thoracic flap (3 patients). The sizes of the transferred flaps were of 6 cm×12 cm-15 cm×28 cm. Eighteen patients had direct suture of donor sites and 7 were reconstructed with medium-thick skin grafts. The 2 teams then worked together: to cover the defect with the flap, anastomose the artery carried by flap with the posterior tibial artery of the bridge, flap dominant vein with the great saphenous vein of the bridge and flap, and non-dominant vein with the dominant posterior tibial vein of the bridge respectively, and then suture the wounds. Stage II surgery: it was carried out at 5-6 weeks later. In which, pedicle of the flap bridge was disconnected, and the great saphenous vein (including 3-4 venous valves) with a ≥10 cm in length, was dissected to the proximal end of the flap bridge. The end of it was anastomosed with the great saphenous vein proximal to the end of the affected limb. The flap bridge and the vascular bundle behind the tibia were restored and replanted. Postoperative wound management and follow-up were carried out and LEFS were collected.Results:All 25 patients were included in the postoperative follow-up for 6-18 (average 10.5) months. CDU showed that the reconstructed great saphenous veins were unobstructed. All the ulcers were cured and the appearances of the calfs were satisfactory with good texture, and without recurrence of ulcers. There was a linear scar in donor sites and without scar formation at the sites of skin graft and skin extraction. The last LEFS was 67.32 points±8.43 points. All patients returned to normal life and work.Conclusion:Classification of traumatic calf ulcer into 3 types has a theoretical basis in selection and modification of flap bridging surgery. The surgery has characteristics of individuation and modularisation, it not only benefits the reconstruction of ulcerative defect, but also prevents the recurrence of ulcer. It is a safe and effective surgical procedure with clinical proof.