Objective To investigate the influence of CO_2-insufflation on the expression of some adhesion molecules.Methods With an artificial pneumoperitoneum model in vitro, SW1116 colon carcinoma cells were exposed to CO_2-insufflation at different pressure (6, 9, 12 and 15 mmHg) for 1 hour. Control groups were exposed to room air. E-cadherin, ICAM-1, E-selectin, CD44 and CD44v6 were measured 0, 12, 24, 48 and 72 hours after CO_2-insufflation using flowcytometry and the mRNA level of these adhesion molecules were detected by real-time PCR.Results In transcription level the expression of ICAM-1 and CD44v6 on SW1116 cell line increased significantly in 6mmHg CO_2-insufflation group, in 9mmHg and 12mmHg group, E-cadherin,CD44 and CD44v6 increased significantly, and when the pressure was 15mmHg, CD44v6 increased (P

Objective To investigate the effect of CO_2-insufflation on mRNA expression of adhesion molecules of colorectal cancer cell lines. Methods With an artificial pneumoperitoneum model in vitro, SW1116 human colon carcinoma cell lines were exposed to CO_2-insufflation of different pressure ( 6mmHg , 9mmHg, 12mmHg and 15mmHg) for 1 hour. The mRNA level of E-cadherin, ICAM-1, CD44 and CD44v6 were measured after 0, 12, 24, 48 and 72 hours by real-time RT-PCR. Control cell lines were exposed to room air. ResultsThe mRNA expression of E-cadherin, ICAM-1, CD44 and CD44v6 increased significantly at 0h～48h after the cells were exposed to the continuous CO_2-insufflation for 1 hour(P

Overweight and obesity in children are one of the severe public health crises in China, which is closely related to various health issues during adolescence, such as sexual development, height, internal metabolism, and psychology. Therefore, it is of great significance to deeply understand the influencing factors of childhood obesity and its puberty development, explore the relationship between obesity and puberty development, and intervene in adolescent obesity and predict obesity related diseases in children and adolescents.