Objective To observe the effects of Chinese herbal medicine of Yiqi Huoxue (YQHX) on proliferation and expression of E-CD and invasion of human lung cancer cell lines - small cell lung cancer (NC-H446) in vitro. Methods By cell measured method, immunohistochemistry method, technique of image and Boyden Chamber method, the effects of YQHX or all trans-tretinoin acid (ARTA) on proliferation and expression of E-CD and invasion of NC-H446 cell lines with different time cultured by YQHX or ARTA in vitro were studied. Results The proliferation of NC-H446 were inhibited significantly by 5% YQHX or 10-5 mmol/L ARTA (P0.05). The shape and structure of cell NC-H446 was occurred variant. YQHX enhanced remarkably expression of E-CD of NC-H446 with 36 hour cultured (P 0.05). The quantity of NC-H446 cells crossed Matrigel and polyvinylpyrrolidone-free polycarbonate filter were more than the control group (P 0.05). Conclusion YQHX can inhibit the proliferation, enhance expression of E-CD on NC-H446, and decrease invasion and metastasis of NC-H446.

Objective To study the activity of N acetylglucosaminyltransferase Ⅲ, Ⅳ and V in extrahepatic cholangiocarcinoma and its significance.Methods The activities of N acetylglucosaminyltransferase(GnT) Ⅲ, Ⅳ and Ⅴ in extrahepatic bile duct carcinoma (EBDC) of 15 cases were determined by reverse phase HPLC (high performance liquid chromatography), and results were compared with benign biliary diseases in 15 cases. Results Compared with normal control, that the specific activities of GnT Ⅲ and GnT Ⅴ increased by 3 3 and 13 5 fold respectively in all of the EBDC samples(all P

0.05). The pulmonary function in M3 after radiotherapy were lower than that in M1 after radiotherapy of control group (P

Objective To observe the effect of Yiqi Huoxue Recipe on the expression of TGF-?1 and ?-IFN in rats with radiation-induced lung injury in different radiation time, and discuss the prevention and curative effect of Yiqi Huoxue Recipe and its possible action mechanism. Methods One hundred and twenty Wistar rats were randomly allocated into irradiation group (n =30), irradiation with Yiqi Huoxue Recipe-treated group (n =30), irradiation with ambroxol-treated group (n =30) and normal control group (n =6). The former three groups were irradiated at right hemithorax with a dose of 30 Gy (5 Gy per week). Animals were followed for 26 weeks after the first irradiation. The levels of serum TGF-?1 and ?-IFN of 6 rats in each group were assayed by ELISA at the end of 4th, 6th, 8th, 12th and 26th week after the first irradiation. Results In Yiqi Huoxue Recipe-treated group, the levels of serum TGF-?1 were significantly lower from the 4th week than that of the irradiation group (P0.05). Conclusion Yiqi Huoxue Recipe can inhibit the excretion of TGF-?1 and promote the excretion of ?-IFN. It suggested that Yiqi Huoxue Recipe might play an important role in the prevention and curation of radiation-induced lung injury.

Objective To investigate the expression of muhidrug resistance-related protein 1 (MRP1),lung resistance-related protein(LfuP)and glutathione S-transferase-π(GST-π)in carcinoma of the gallbladder and cholangiocarcinoma. Methoils MRP1,LRP,GST-πwere measured in experimental group(18 cases of carcinoma of the gallbladder,36 CSSeS Of cholangiocarcinoma)and control group(13cases of cholecystitis and cholangeitis)by immunohistochemistry.Statistical analysis used chi-square test and spearman test. Results The positive rate of MRP1,LRP,GST-π in carcinoma of the gallbladder and eholangiocarcinoma were 72%(13/18),78%(14/18),61%(11/18)and 86%(31/36),75%(27/36),69%(25/36),respectively,significantly higher than those of 23%(3/13),23%(3/13),23%(3/13)(X2=4.5,P＜0.05)in control group.The expression of LRP[93%(13/14)]in pafients＞60 years old was significantly higher than 64%(14/22)in patients younger than 60 yrs old(x2=3.9,P ＜0.05).In addition,their expression was not related to gender,age,staging,tumor differentiation and lymph node metastasis(P＞0.05).The expression of MRP1 was related with tllose Of GST-π,Spearman correlation coefficient=0.569(P＜0.05).Conclusions MRP1,LRP,GsT-π were over expressed in various degrees in carcinoma Of the gallbladder and cholangiocarcinoma witllout chemotherapy.and related to the primary muhidrug resistance Of cholangiocarcinoma and carcinoma of the gallbladder.

Objective:Eighteen inorganic elements were measured from the rhizoma and fibrous root of Polygonatum Cyrtonema Hua. The contents of K, Fe,Mg,Ba,Cu,Mn and Bi of them in rhizoma were higher than that of fibrous root, where as the contents of Na,Al,Ca,Ge,P,Zn and Sr elements in rhizoma were lowere than that of fibrous. Both contained As,Hg,Pb and Cd elements. In the meantime, 16 mino acid were determined. The total content of them was 8.92% for rhizoma and 9.61% for fibrous. Cystine, cysteine, tryptophan and ornithine couldn't be detected.

ObjectiveTo explore the clinical significance and characteristics about terminal sialyl residues linked to N-glycans in the tissue and bile glycoproteins from bile duct carcinoma. MethodsThe mRNA expression of sialyltransferases, including ST3Gal-Ⅲ and ST6Gal-Ⅰ, was detected in the tissue extracts of 35 cases with bile duct carcinoma(BDC) and 35 cases of benign biliary disease (BBD) by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR). With the lectin of WGA(Triticum valgaris agglutinin), which can recognise the terminal sialyl residues in N-glycans, all the specimens of BDC and BBD were subject to lectin histochemical staining, followed by computer-aided digital analysis. Lectin dot-blotting with horseradish peroxidase-labeled WGA was performed for the bile samples from each of the 35 cases of BDC and BBD. ResultsThe mRNA levels of ST3Gal-Ⅲ and ST6Gal-Ⅰ elevated in BDC(P

Objective To investigate the appearance and characteristics of hepatic tuberculosis lesions on low mechanical index contrast-enhanced ultrasound. Methods Eight patients with hepatic tuberculosis lesions were studied with conventional ultrasound and contrast-enhanced ultrasound. Lesions,distribution.size,echogenieity,blood flow,enhancement phase,enhancement patterns and intensity were studied. ResuIts Fifteen lesions were found in eight patients. On conventional ultrasound,mean size of the lesions was(21.7±10.7)mm×(30.3±22.4)mm,and 66.7%(10/15)lesions located under liver capdule,and 33.3%(5/15)were in hepatic parenchyma.All lesions were hypoechoic except the largest one (50 mm x 102 mm)which demonstrated heterogeneous.Doppler signals were not demonstrated in all 15 lesions.After administration of SonoVue,satisfied image data were obtained in 13 lesions for aterial phase,and 15 lesions for portal venous phase and parenchyma phase.84.6%(11/13)of the lesions demonstrated hyperenhancing on aterial phase,mean enhancing start time was(8.6±1.6)s,ranging from 5 s to 10 s.and mean enhancing end time was(25.6±2.8)s,ranging from 20 s to 29 s. Of 13 lesions,6 lesions demonstrated circle periphery hyper-enchanCement,5 lesions demonstrated whole hyper-enchancement,and 2 lesions appeared hypoenhancement on arterial phase. All lesions demonstrated either hypoenhancing or non-enhancement on portal venous phase and parenchyma phase. Conclusions Low mechanical index contrast-enhanced ultrasound can reveal the blood flow status of hepatic tuberculous lesions and will facilitate the diagnosis of hepatic tuberculosis.