Objective To investigate the effect of interleukin-6 (IL-6) on the growth of human lung cancer in vivo as well as in vitro.Methods To examine the mRNA level of IL-6 receptor (IL-6R) in high-metastatic human lung giant cell carcinoma cell line PG by means of reverse transcription polymerase chain reaction (RT-PCR). To assess the existence of IL-6 receptor complex (including IL-6R and gp130) with the treatment of PG cells by use of recombinant human IL-6 (rhIL-6), recombinant human oncostatin M (rhOSM), and recombinant human leukemia inhibitory factor (rhLIF), respectively. To detect the expression of IL-6 by Northern blotting hybridization and bioactive assay. To identify the effect of IL-6 secreted by PG cells by use of IL-6 and IL-6R antisense oligodeoxynucleotides (ODNs), and specific neutralizing antibody to IL-6. To document the influence of IL-6 on PG cells growth in vivo through the strategy of the transfection of expression vector inserted antisense IL-6 cDNA.Results RT-PCR analysis revealed that PG cells expressed IL-6R mRNA. Any one of the recombinant cytokine IL-6, OSM and LIF stimulated the growth of PG cells in vitro in a concentration-dependent manner. These results demonstrated IL-6 receptor complex exist in PG cells. At the same time, PG cells expressed IL-6 mRNA and secreted bioactive IL-6. Both IL-6 antisense ODNs and IL-6R ODNs inhibited PG cells proliferation. Treatment of PG cells with IL-6 antibodies reduced the growth of PG cells in vitro. PG cells transfected with IL-6 antisense expression vector showed a decreased growth in nude mice.Conclusion IL-6 functions as an autocrine growth stimulator for PG cells in vivo as well as in vitro.

In recent years,with the continuous development of information technology in the big data environment,the requirements for hospital medical record information management have been increasing.Combined with the reform and innovation of computer technology,the traditional medical record information management mode is gradually transforming towards simplicity,convenience,and user-friendliness.This article mainly discusses the importance of hospital medical record management informati-zation in the big data environment and the problems existing in the information management process.It proposes measures to strengthen the construction of software and hardware facilities,enhance network information technology,and increase training for professional personnel to cope with these issues.Furthermore,comprehensive efforts should be made to strengthen the informati-zation of medical record management,improve the integration capacity and utilization rate of medical record information,and pro-mote the high-quality development of hospitals.