Objective:To investigate the relationships between Leu72Met polymorphism ofpreproghrelin gene and type 2 diabetes mellitus (T2DM)in Han ethnics ofGuangzhou.Methods:We conducted a case-control study using 95 non-T2DM healthy individuals as controls (the control group)and 102 individuals diagnosed with T2DM as patients(the T2DM group)from populations of Han ethnics in Guangzhou,in order to detect genotypes of Leu72Met polymorphism of preproghrelin gene within each group,and to compare the differ-ences of genotypic and allelic frequencies between the two groups.Various molecular biology techniques were employed in the study in-cluding target gene segments amplification using the polymerase chain reaction(PCR).After amplification,digestions of PCR products were carried out using restriction enzyme BseNⅠand fragments obtained were detected using Native-PAGE in conjunction with a highly sensitive silver stain method.Results:In the healthy control group,the genotypes CC and AC were detected in 68 and 27 individuals,re-spectively,while no genotype AA was detected in this group.Their corresponding genotypic frequencies were 0.716,0.284,and 0,re-spectively.In the T2DM group,the genotypes CC,AC,and AA were detected in 71,25,and 6 cases,respectively.Their corresponding genotypic frequencies were 0.696,0.245,and 0.059,respectively.Allele frequencies detected were in equilibrium with the Hardy-Wein-berg equation.However,there were no significant differences between the control group and the T2DM group in terms of genotypic and al-lelic frequencies.Conclusion:There are no significant relationships between Leu72Met polymorphism of preproghrelin gene and T2DM in Han ethnics of Guangzhou.However,genotype AA was detected in the T2DM group but not in control,which suggested that allele A may play certain roles in T2DM development.Further investigations certainly would be worth pursuing.

OBJECTIVE:To analyze the chemical constituents of Galanga Resurrectionlily Rhizome from Hainan province. METHODS: Galanga Resurrectionlily Rhizome volatile oil was extracted by wet distillation method and determined by GC-MS, and the relative content of different constituents was determined by chromatographic peak area normalization method. RESULTS: For Galanga resurrectionlily rhizome volatile oil, 21 chromatographic peaks were separated and 19 chemical constituents were detected. The chief constituents of the volatile oil were Ethyl-p-methoxycinnamate(49.118%), ethyl cinnamate (19.323%), pentadecane (15.018%) and ?-careen (5.270%). CONCLUSION: This method provided not only data for the further study of the physiological activity and action mechanism of Galanga Resurrectionlily Rhizome volatile oil but also scientific basis for the development and application of Galanga Resurrectionlily Rhizome volatile oil.

Objective　To investigate genes involved in cancer metastasis, mRNA differential display was used to compare the levels of gene expression in two cell sublines derived from human giant cell carcinoma of lung (PG) which had different metastatic potentials. Methods　Using in vivo tumorigenicity and a spontaneous metastasis assay in nude mice, two sublines (BE1, LH7) from human giant cell carcinoma of lung (PG) with different metastatic potentials were isolated and characterized. mRNA differential display was used to compare the levels of gene expression between them and the obtained results were confirmed by Northern hybridization. Results　One differentially expressed band was nearly identical (99% homology) to Ras-GTPase-Activating protein SH3 domain binding protein (G3BP). G3BP displayed a strong expression in LH7 (non-metastatic in recipient nude mice) and a very weak expression in BE1 (100% metastatic frequency). The same different expression level of G3BP was detected in Northern hybridization with another panel of cell sublines with different metastatic potentials (established in our lab) derived from human prostate carcinoma cell line PC-3M. Conclusion　Our results indicate that G3BP was implicated in cancer metastasis because of its differential expressions in the two panels of cell sublines with different metastatic potentials.