Objective　To investigate the expressions and significance of protein kinase C (PKC) subtype α, β2 and ε in the pathogenesis of glomerulonephritis. Methods　Immunohistochemical assays was used to examine the expressions of PKC α, PKC β2 and PKC ε in biopsy specimens of kidney from patients with mesangial proliferative glomerulonephritis (MsPGN) （n=16），nonproliferative golmerulonephritis (nonPGN) (n=12)，and in structurally normal kidneys (n=6). Results　The PKC β2 expression in the glomerular was significantly higher in MsPGN patients than in nonPGN or normal kidney groups. Conclusion　PKC β2 has a close association with MsPGN and might take part in the initiation of this renal disease.