Objective To study diagnostic value of MRI in the mitochondrial encephalomyopathy about children.Methods 16 patients of mitochondrial encephalomyopathy in children had been examinatd with MR,proved by patholigy and biochemical laboratory examinations from 1996 to 2000.All of informations were retrospectively analysed.Results Brain parenchymal lesions in all cases were low signal intensity on T 1-weighted and high signal imtensity on T 2-weighted images.16 patients with mitochondrial encephalomyopathe of children,9 patients of them had only the deep gray matter involved,4 patients had both the deep gray matter and the cerebral cortex involved simultsneously,2 patients had with involvement of cortex and subcortical white matter,1 patient had exclusitvely involvement of white matter.Conclusion The varied clinical and MRI findings are the characteristic of mitochondrial encephalomyopathy,MRI has better helping to the diagnosis of mitochondrial encephalomyopathy in children.

Objective To explore MRI diagnostic value of intervertebral disc infection.Methods 10 cases of intervertebral disc infection proved by operation and pathology were restrospectively analysed.Results 15 intervertebral discs were invalved in 10 patients.Among them,lumbar vertebral discs were only invalved in 7 cases,lumbar and sacrum vertebral discs were in 3 cases simultaneously.The involved discs were long T 1 and long T 2 signal intensity in 8 cases equal T 1 and short T 2 signal intensity in 2 cases.The soft tissue around vertebral was involved.The involved discs and soft tissue were enhanced in varied degrees on Gd-DTPA contrast-enhanced scan in 6 cases.Conclusion MRI is of important value in diagnosis of vertebral disc infection.

Objective To explore MRI features of spinal arachnoid cysts.Methods The MRI appearances of 20 cases with spinal arachnoid cysts proved by surgery and pathology were retrospectively analyzed.There were 8 male and 12 female,they were conventionally scaned by MRI,12 cases of them were added Gd-DTPA contrast-enhanced scan.Results There were one nearilemma cysts,5 extradural arachnoid cysts,6 subdural arachnoid cysts,8 sacral arachnoid cysts.The signal intensity of cysts were long T_1 and long T_2 similar to that of cerebrospinal fluid signals and no enhanced on contrast MRI.Conclusion MRI is the best method in diagnosing the spinal arachnoid cysts.Contrast-enhanced scan is helpful for diagnosing and distinguishing of spinal arachnoid cysts.

ObjectiveTo explore the imaging characteristics and the clinical and pathological diagnosis of gliomatosis cerebri in order to raise the awareness of the disease.MethodsThe images of 26 patients with gliomatosis cerebri were analyzed retrospectively.ResultsEight patients were treated with partial excision and 18 patients got stereotactic biopsy.The radiography examination included the plain and enhanced scan of CT and MRI.Twelve patients received 1H magnetic resonance spectroscopy (MRS).The disease in all the patients involved at least two cerebral lobes.There were 22 cases whose CT and MRI manifestations showed that there were large-sheet symmetry low-density image in brain and slightly long T1 and long T2 abnormal signals.And the enhanced scan showed that 20 cases had no obvious enhancement,2 cases had slight enhancement.There were 4 cases with large-sheet asymmetry lesions and no enhancement in enhanced scan.1H MRS examination of 12 cases showed that choline( CHO )/creatine(Cr) and CHO/N-acetyl aspartic acid (NAA) increased,NAA/Cr decreased with different degree,and 8 patients had lactate peak.ConclusionsMRI is the first choice to diagnose gliomatosis cerebri.1H MRS is very helpful for the diagnosis and pathological classification of the disease.MRI combined with biopsy can give out a definite diagnosis.

Objective To investigate the operative technique and outcome of the expanded flap to repair the full thickness defects of nose and upper lip simultaneously.Methods Three kinds of ex panded flaps were chosen to reconstruct the full thickness defects of nose and upper lip.They were designed according to the location and the size of the defects,and also the circumstances of the donor site.Treatment effectiveness were observed by long-term follow-up.Results 7 patients were treated with the expanded flap.All cases received satisfactory results with no complications such as flap necrosis.And for male patients,the frontal expanded flap could reconstruct upper lip and beard simultaneously that reached better shape.Conclusions It is an ideal approach of repairing nasal-lip defects by the tissue expanded flap.

OBJECTIVETo investigate the effects of Matrine on apoptosis of fibroblasts and the expression of apoptotic modulation related protein in the hypertrophic scar.

METHODSHypertrophic scar was produced on the ear of 24 New Zealand white rabbits, which were employed as the model, and were randomly and equally divided into control (CC) and Matrine (M) groups (12 in each group). Matrine (50 g/L) was injected into the ear scar in M group and with normal saline in C group once every four days. At 2, 4, 6, 8 and 12 weeks after the injection, the apoptotic fibroblast count in the scar was determined by TUNEL method, and the expressions of apoptosis related modulation proteins p53, bcl-2, bax were detected by immunohistochemistry method.

RESULTSThe apoptotic fibroblast count was much larger in M group than that in C group at all test time points (P < 0.05). Furthermore, the bax expression was increased and that of p53 and bcl-2 was decreased significantly in M group. In adding, the scar became flat in M group.

CONCLUSIONMatrine might obviously enhance the fibroblast apoptosis in rabbit ear hypertrophic scar, and up-regulate the expression of apoptosis related modulation protein bax and down-regulate the expression of p53 and Bcl-2.

Alkaloids ; pharmacology ; Animals ; Apoptosis ; drug effects ; Cicatrix, Hypertrophic ; metabolism ; pathology ; Female ; Fibroblasts ; drug effects ; Immunohistochemistry ; Male ; Proto-Oncogene Proteins ; analysis ; Proto-Oncogene Proteins c-bcl-2 ; analysis ; Quinolizines ; Rabbits ; Tumor Suppressor Protein p53 ; analysis ; bcl-2-Associated X Protein