Objective:To compare the microbial count method described in Chinese Pharmacopoeia ( ChP) 2010 edition and 2015 edition. Methods:The bacterial count and total aerobic microbial count for 15 samples of Jingfang granule with the same batch were tested respectively by the method described in ChP 2010 edition and 2015 edition, the average number, uncertainty, colony distribu-tion range of samples and qualified rate from the two testing items were analyzed and compared. Results:The average number of colo-nies for the bacterial count and total aerobic microbial count was 720 and 830 cfu·g-1 , the expand uncertainty of 95％ confidence in-tervals was 0. 067 and 0. 061, the colony distribution range of samples was 620-840 cfu·g-1 and 720-960 cfu·g-1 , and the qualified rate was 90％ and 100％, respectively. Conclusion:The microbial count method described in Chp 2015 edition is more sensitive with more reasonable result evaluation, which can guarantee the stability of inspection reports.

Objective To evaluate in vitro antifungal activities of five clinically common antifungal agents against Geotrichum silvicola,and to observe morphological changes of Geotrichum silvicola after treatment with terbinafine.Methods Five antifungal agents commonly used in clinic,including terbinafine (TER),amphotericin B (AmB),flucytosine (FC),fluconazol (FCZ) and itraconazole (ICZ),were tested in this study.According to the National Committee for Clinical Laboratory Standards (NCCLS) M27-A2 document and relevant literatures,a susceptibility test was performed to determine the minimal inhibitory concentration (MIC) values of the five antifungal agents against G eotrichum silvicola.Scanning electron microscopy (SEM) was used to observe morphological changes of Geotrichum silvicola treated with different concentrations of TER.Results The MIC values against Geotrichum silvicola were as follows:TER,0.01 μg/ml;AmB,0.4 μg/ml;FC,2 μg/ml;FCZ,2.69 μg/ml;ICZ,0.25 μg/ml.Geotrichum silvicola was sensitive to TER,AmB,FC and FCZ,and sensitive to ICZ in a dose-dependent manner.SEM revealed that after TER treatment,the surface of Geotrichum silvicola became rough and shrinking with irregular defects and holes,and even gave a fragment-like appearance.Conclusions Among the five tested antifungal agents,TER shows the strongest activity against Geotrichum silvicola.Within a certain range,the higher the concentration of TER,the more serious the damage to Geotrichum silvicola.

[Objective] This prospective study was conducted to assess the influence of overnight orthokeratology (OK) on low,middle and high myopia in southern Chinese teenagers.[Methods] Fifty-four subjects (107 eyes,age 11.65 ± 2.44 years,mean±SD)who matched the inclusion criteria for OK were enrolled in the study.The subjects were divided into three groups according to the different refraction baseline (low myopia group:0.75 ～ 3.00 D,51 eyes;middle myopia group:3.25 ～ 5.00 D,36 eyes;high myopia group:5.25 ～ 9.00 D,20 eyes).Refraction,central corneal thickness,corneal keratometry,anterior chamber depth and axial length (AL) were measured at baseline and after 1 years using ocular biometry.The changes were evaluated and compared among the groups.Results were analyzed by SPSS 17.0 software.[Results] Fifty-four subjects (total 107 eyes) completed the 1-year follow-up examinations.At baseline,the spherical equivalent refractive error (SER) was-2.15 D ± 0.63 D,-4.20 D ± 0.54 D,and-6.63 D ±1.18 D in three groups.The axial length was (24.50-± 0.66) mm,(25.16-± 0.65) mm,and (26.35 ± 0.87) mm in the three groups,respectively.There were no significant differences among the groups in initial central corneal thickness,anterior chamber depth,central,flat and steep kerotometry.The increase in axial length during the 1-year study period was (0.22 ± 0.27) mm,(0.09-± 0.22)mm and (0.02-± 0.22) mm in three groups,respectively,and the difference was significant between low myopia group and middle myopia group (P =0.04),low myopia group and high myopia group (P =0.008),whereas no significant difference was found between middle myopia group and high myopia group (P =0.35).Spherical equivalence decreased dramatically in three groups after one year (all P ＜ 0.0001).And the difference was significant between low myopia group and middle myopia group (P ＜ 0.0001),low myopia group and high myopia group (P ＜ 0.0001).Central,flat and steep kerotometry after one year became flatten in all group (all P ＜0.0001).There were no significant differences in the change of central corneal thickness,anterior chamber depth and corneal diameter.[Conclusions] OK lens is more effective in middle and high myopia control than in the low myopia control.The change in corneal keratometry may be one of the main factors influencing the OK treatment effect.

Objective:To establish the uncertainty evaluation method for total aerobic microbial count of Jingfang granule. Meth-ods:According to Chinese Pharmacopoeia (2015 edition, volume IV), the total aerobic microbial count of 20 samples of the same batch of Jingfang granule was detected. National specification of measuring instruments JJF1059.1-2012 was used to perform the uncer-tainty evaluation on the total count of aerobic microbial type A and type B,and the combined uncertainty and the extended uncertainty were calculated. SPSS statistics 19.0 software was used to analyze the normal distribution of data. Results:The combined standard was 0.043 9, the expanded measurement uncertainty was 0.088(k=2),the colony distribution range of the samples was 690-1 000 cfu· g-1,and the data was normal distribution. Conclusion:The established method for uncertainty assessment is simple and convenient, and the results of new test samples can be added. New range of uncertainty can be obtained by recalculating the standard deviation of the combined samples.