Objective To investigate the application of optical coherence tomography (OCT) in traumatic macular disease.MethodsOCT examination was carried out and analyzed in 56 eyes of 56 cases with traumatic macular disease.ResultsThe OCT examination showed that there were 23 cases of whole macular hole, 3 cases of lamellar macular hole, 3 cases of epi-macular membrane, 8 cases of macular hemorrhage, 5 cases of serous neuroretinal detachment, 4 cases of hemorrhagic retinal pigment epithelium detachment, 4 cases of macular edema and 6 eyes of macular neuroretinal thinning.ConclusionOCT plays an important role in diagnosis, monitoring and investigation of pathogenic mechanisms for traumatic macular disease.

0.05). The corresponding 1-, 2- and 3-year loca l r ecurrence rates were 4.4%, 8.9%, 13.3% and 9.8%, 26.8%, 32.6% (P 0.05). Conclusions Simultaneous small-field boosting radiotherapy can obviously reduce t he local recurrence and improve the survival without causing more toxicities in the treatment of soft tissue sarcoma in extremities.

Objective To observe the radioprotective effect of low dose mitomycin C(MMC) on chest carcinoma. Methods 100 cases of chest carcinomas confirmed by histologic or cytologic diagnosis, including, esophageal carcinoma 54 cases, lung cancer 46 cases, were randomized into study group and control group, 50 cases in each group. The study group was treated with low dose MMC (0.002 mg/kg iv one time per week, about 5 ~ 7 times in the course) during routine radiotherapy, the control group was treated with routine radiotherapy only. Results All of 100 cases completed the treatment. Acute radiation- induced esophagitis of study group and control group was 30 % and 48 % respectively (?2=3.897,P =0.048). Acute radiation- induced pneumonia of study group and control group was 4 % and 16 % respectively (?2 =4.001,P =0.045). Hematologic toxicity of study group and control group was 50 % and 48 % respectively (?2=0.208, P =0.648). Response rate of study group (84 %) was obviously higher than that of control group (68 %) (?2 =4.089, P =0.043). Conclusions Low dose MMC combining with chest carcinoma's radiotherapy can obviously reduce acute radiation- induced esophagitis and pneumonia, without obvious hematologic toxicity, meanwhile increase radiation effect.

Objective To evaluate the therapeutic effectiveness and toxicity of low-dose cisplatin chemotherapy combined with three-dimensional conformal radiotherapy for mid-advanced esophageal cancer.Methods 89 patients with advanced esophageal cancer either who were inoperable or refused operation were randomized into two groups: 45 cases in the study group and 44 cases in the compared group. The study group

Objective To explore the use of Ultrasonic Biomicroscopy(UBM)for peri-operative examination of traumatic cyclodialysis.MethodsUBM was used in 33 eyes of 33 cases who were diagnosed as traumatic cyclodialysis.The morphologic characters were observed peri-operatively.ResultsCiliary detachment of 360° was verified in the 33 eyes with cyclodialysis of more than 2 clock hours.1 month after cyclopexy,the space of ciliary detachment disappeared or diminished and all reattached 3 months after operation.ConclusionUBM is safe and effective for checking traumatic cyclodialysis.

Objective To compare and observe the effect and side effect of two different kinds of simulation mode for nasopharyngeal carcinoma conventional radiotherapy. Methods 147 cases were randomly divided into the study group (74 cases, CT simulation) and the control group (73 cases, X-ray radiography simulation). In the study group three-dimensional treatment planning system (IPS) was used to draw target volume and organ at risk, and then beam eye view (BEV) function was utilized to design treatment plan and subsequent plan modulation. In the control group X-ray simulation radiography was used. Two groups of plan design and subsequent plan modulation mode were exactly the same, and the radiotherapy were performed on the same accelerator. Results For these two groups, there were 5-59 months follow-up. The recent therapeutic effect of nasopharyngeal carcinoma primary site and cervical lymph node metastasis was regular,and no statistical significance (P >0.05). For the study group 3-year local recurrence rate was 5.4 % (4/74) while the control group was 16.4 %(12/73), and there was obvious statistical significance between two groups. About one year and three year accumulated survival rate, the study group was 98.5 %, 77.4 %, and for the control group was 92.9 %, 64.7 %. Two groups survival curves were examined by Log-rank test: the survival curves showed statistical significance between two groups(P <0.05). In control group, stage II and III radiotheray reaction of oralis mucosa was significantly increased compared with that of the study group (P < 0.05). There were two cases radioactivity cerebral palsy and one case optic nerve damage in the control group, but no serious dermatosis, soft tissue fibrosis and radioactivity posterior cranial nerve damage. Conclusion The CT simulation which is applied to nasopharyngeal carcinoma conventional radiotheraphy could improve therapeutic effect and reduce cancer recurrence and radiation side effect.

Objective:To explore the effect of rapamycin on 1-methyl-4-phenylpyridinium iodide (MPP+ )-induced activation of Nod-like receptor protein 3 (NLRP3) inflammasome in microglia.Methods:The BV2 microglia cells were divided into control group, model group and rapamycin group.The model group and rapamycin group were treated by MPP+ to activate NLRP3 inflammasome, and rapamycin group was pretreated with rapamycin.Quantitative real-time PCR (RT-qPCR) was used to detect the mRNA levels of NLRP3, apoptosis-associated speck-like protein (ASC) and caspase-1.Immunofluorescence was used to detect the protein expression of NLRP3 and interleukin-1β (IL-1β). Western blot was carried out to assess the protein expression of NLRP3, ASC, caspase-1, beclin1 and microtubule-associated protein 1 light chain 3 (LC3).Results:The mRNA levels of NLRP3, ASC and caspase-1 in model group were higher than those in control group ( t=4.825, 3.015, 5.853, all P<0.05). The mRNA levels of NLRP3 and caspase-1 in rapamycin group were lower than those in model group ( t=2.75, 2.89, both P<0.05). In model group, the protein expressions of NLRP3 (1.54±0.22), ASC (1.02±0.13) and caspase-1 (1.42±0.30) were higher than NLRP3 (0.66±0.15), ASC (0.41±0.14) and caspase-1 (0.70±0.10) in control group ( t=5.653, 5.602, 3.964, all P<0.01), while the protein expression of beclin1 (0.28±0.09) and LC3II/LC3I ratio(0.69±0.14) were lower than beclin1 (0.60±0.11) and LC3II/LC3I (1.29±0.23) in control group ( t=4.010, 3.982, both P<0.01). The protein expressions of NLRP3 (0.80±0.18) and ASC (0.68±0.14) in rapamycin group were lower than those in model group ( t=4.413, 3.077, both P<0.05), while the protein expression of beclin1 (0.65±0.20) and LC3II/LC3I ratio(1.42±0.36) were higher than those in model group ( t=2.965, 3.278, both P<0.05). Conclusion:MPP+ activates NLRP3 inflammasome and impairs autophagic function in microglia.Rapamycin inhibits MPP+ -induced activation of NLRP3 inflammasome by restoring autophagic impairment in microglia.

OBJECTIVE: To investigate the profile and potential significance of PTEN and NBS1 mutations among patients with familial or at early onset breast cancer in Hunan province.
 METHODS: A total of 131 breast cancer patients with familial history or suffered from breast cancer at the age of less than 35 years old were included in this study. A comprehensive phosphatase and tensin homolog (PTEN) and nibrin (NBS1) mutation analysis was performed through denaturing high performance liquid chromatography (DHPLC) and subsequent DNA direct sequencing.
 RESULTS: Among 131 patients, a reported mutation IVS4+109insTCTTA in PTEN gene were identified in two patients. The mutation frequency of IVS4+109insTCTTA was 1.15%. Two mutations in PTEN gene, 225 A>C (Thr 160 Pro) and IVS5+13T>C, was firstly discovered. Another reported missense mutation was rs121909229 G>A (Arg 130 Gln). Three mutations were detected in NBS1 gene, of which IVS6+43A>G and IVS6+127A>G were firstly discovered and another reported synonymous mutations was rs1805794 G>C (Glu 185 Gln).
 CONCLUSION The novel mutations in PTEN and NBS1 might be specific to the familial and early-onset breast cancer of Chinese Hunan population.
Adult ; Asian Continental Ancestry Group ; Breast Neoplasms ; genetics ; Cell Cycle Proteins ; genetics ; China ; DNA Mutational Analysis ; Female ; Humans ; Mutation ; Nuclear Proteins ; genetics ; PTEN Phosphohydrolase ; genetics