1.Transplantation of autologous bone marrow mesenchymal stem cells improves neovascularization in lower limb ischemia in rabbits
Rong HUANG ; Yuzhen LIANG ; Bingfeng LU
Journal of Medical Postgraduates 2015;(7):706-710
Objective Stem cell transplantation is a new approach to the treatment of lower limb ischemia ( LLI) .This study was to investigate the therapeutic effect of the transplantation of autologous bone marrow mesenchymal stem cells ( BM-MSCs) in the treatment of LLI. Methods We established the left LLI model in 12 New Zealand rabbits and divided them into a control and a trial group of equal number.The control animals were injected with DMEM, while the rabbits in the trial group with autologous BM-MSCs, into the ischemic skeletal muscle.Four weeks after injection, we performed CT angiography and perfusion imaging of both lower limbs of the rabbits and conducted HE staining of the paraffin sections of the skeletal muscle of the ischemic limbs. Results Dynamic ob-servation revealed different degrees of ecderon necrosis in 2 of the LLI models in the control group, even with toenail coloboma, but no necrosis in the trial group except for some slight muscular atrophy. Both collateral arteries and blood perfusion were obviously increased in the ischemic lower limbs in the trial than in the control group.HE stai-ning showed a significantly higher density and percentage of capillaries in the skeletal muscle fibers in the former than in the latter ([6.500 ± 1.049]/HP vs [3.670 ±0.816]/HP, [9.68 ±0.56]%vs [5.87 ± 0.86]%, P<0.01), with no necrosis in either group, nor hematoma, bony tissue, or fibroid tumor in the trial group. Conclusion Autologous transplantation of BM-MSCs can improve neovascularization in ischemic lower limbs in rabbits and can be used as a safe and effective treatment of limb ischemia.
2.Small Hepatocellular Carcinoma: The Correlation of Blood Supply Type on Multiphase Spiral CT with Immunohistochemical Findings
Liling LONG ; Bingfeng LU ; Zhongkui HUANG
Journal of Practical Radiology 1991;0(03):-
0.05).Singnificant difference of p21 positive rates was showed between tumor lack of blood supplies and arterial blood (?
3.Small Hepatocellular Carcinoma:Multivariate Regression Analysis of Correlation of Pseudocapsula Completion on Multiphase Spiral CT with Histopathological and Immunohistochemical Findings
Bingfeng LU ; Zhongkui HUANG ; Liling LONG
Journal of Practical Radiology 1996;0(04):-
Objective To study the relationship among SCT features of tumor margin and tumor size, immunohistochemical findings, grade of cell differentiation, and to explore the potency of tumor marginal features on multiphase spiral CT for evaluating biological behavior of small hepatocellular carcinoma (SHCC).Methods Multiphase spiral CT scans were performed in 30 patients with 33 lesions of SHCC (≤3.0 cm in diameter) confirmed histopathologically. Of these patients,26 were male and 4 were female. Immunohistochemical and HE staining were performed in surgical samples of all patients with SHCC. All samples' size was measured and the number of masses in each patient was counted.Results Of all masses, 6 were complete capsule, 15 incomplete capsule and 12 non-capsule. In multiple linear regression analysis, the pseudocapsula presentation and completion of SHCC on SCT were positively related with tumor size and VEGF expression, negatively with CD34 and p21, and were not in association with p53 and histological grade. Tumor size was the strongest contributor to pseudocapsula completion(R=0.5115), and then VEGF(R=0.2917), CD34(R=-0.2981) and p21(R=-0.2726), which had the similar standardized regression coefficients.Conclusion Tumor size is the strongest relative factor that influenced pseudocapsula presentation and completion of SHCC. VEGF always expressed positively in SHCC with complete pseudocapsula, and negatively in the group of SHCC with incomplete pseudocapsula or without pseudocapsula. In the group of SHCC with incomplete pseudocapsula or without pseudocapsula, p21 always express positively. Neither p53 expression nor histological grade is in association with the pseudocapsula presentation and completion of SHCC on SCT.
4.Study on efficacy and safety of sequential rivaroxaban use in reducing blood loss after applying tranexamic acid in total hip arthroplasty
Fulin LI ; Dong YIN ; Bingfeng MO ; Yu HUANG ; Xiao HUANG ; Qiang LU ; Wenhui LIU
Chongqing Medicine 2017;46(16):2193-2197
Objective To research the efficacy and safety of sequential rivaroxaban use in reducing blood loss after applying tranexamic acid(TXA)in total hip arthroplasty(THA).Methods According to the design by the random control principle,150 pa tients undergoing unilateral primary THA from September 2012 to June 2015 were selected and randomly divided into the group A,B,C,D and E (n=30).The group A did not use TXA,the group B received intravenous drip of 10 mg/kg TXA at 10 min before skin incision,the group Creceived intravenous drip of 15 mg/kg TXA at 10 min before skin incision,the group D respectively received intravenous drip of 15mL/kg TXA at 10 min before skin incision and after 3 h,the group E received intravenous drip of 15 mL/kg TXA at 10 min before skin incision and articular cavity use of 1 g TXA before closing the incision.Oral 10 mg rivaroxaban was given at postoperative 6-12 h when the drainage volume was less than 30 mL/h and then the conventional dose was used until postoperative 35 d.The intraoperative blood loss,postoperative drainage volume,hidden blood loss,blood transfusion rate,postoperative anticoagulation time,time of removing drainage tube,postoperative prothrombin time on postoperative 1 d,activated partial thromboplastin time,descend value of hemoglobin,and occurrence rates of postoperative deep vein thrombosis (DVT) and pulmonary embolism (PE) were observed in the group A,B,C,D and E.Results The intraoperative blood loss,postoperative drainage volume,hidden blood loss,blood transfusion rate and descend value of hemoglobin on postoperative 1 d had statistical differences among 5 groups(P<0.05).The are significant differences between the group D and A in the intraoperative blood loss,postoperative drainage volume,hidden blood loss,blood transfusion rate,descend value of hemoglobin on postoperative 1 d,postoperative anticoagulation time and removal drainage tube time(P<0.05).All cases had no symptomatic DVT and PE during the perioperative period and postoperative 3-month outpatient or telephone follow-up.Conclusion Sequential rivaroxaban use after applying TXA during THA perioperative period is safe and effective.Moreover intravenous drip of 15 mL/kg TXA at 10 min before skin incision and after 3 h has most significant effect in reducing bleeding volume during THA perioperative period.
5.Study of HRCT of chest and quality of life of patients with rheumatoid arthritis complicated with interstitial lung disease
Ling LEI ; Cheng ZHAO ; Cundong MI ; Zhijian HUANG ; Jing WEN ; Bingfeng LU
Chinese Journal of Rheumatology 2011;15(10):682-686
ObjectiveTo investigate the changes of high resolution computerized tomography (HRCT) of chest and quality of life and the main correlated factors of HRCT for interstitial lung disease(ILD) in patients with rheumatoid arthritis(RA).MethodsTwenty-six initial treatment patients with RA-ILD were enrolled.The following parameters were noted at baseline,12 and24 weeks for each patient:clinical features,HRCT,quality of life.ANOVA was used for repeated measurement data and stepwise multiple regression analysis were used to analyze the relativity between HRCT and other parameters.ResultsTwentysix patients were included.Twelve male(46%) patients were followed up for 24 weeks and pulmonary infection occurred in 11 patients,so the frequency rate was 42%.After being treated with prednisone and cyclophosphamide for 24 weeks,the HRCT scores were lower than before [(8+6) vs(12±5),respectively] and 16 patients' condition were improved,6 were in stable and 4 had deteriorated disease.For quality of life,the impact scores,symptom scores,activity scores,and total scores of St.George's respiratory questionnaire (SGRQ) were significantly decreased(F=3.783,6.362,4.217,4.426,P<0.05) and all domains of the short form-36 health survey questionnaire(SF-36) had significant improvement after treatment.Stepwise multiple regression analysis showed that the impact scores(P=0.000) and symptom scores(P=0.001) of SGRQ,vitality (P=0.012) of questionnaire,globulin (P=0.027) in prior treatment and symptom scores (P--0.001 ) of SGRQ,course of disease (P=0.002),MRC score (P=0.011),vitality (P=0.036) of SF-36 questionnaire in post-treatment were the main correlated factors with HRCT features.ConclusionMale RA patients are prone to develop ILD and RA-ILD is susceptible to pulmonary infection.After early treatment,HRCT and quality of life in most patients can be improved.Respiratory symptoms,severity of dyspnea,globulin level,course of disease and vitality of patients are significantly correlated with HRCT.
6.Correlation between Urine HCMV Virus Load and Liver Function Indications among HCMV-Infected Infants
Erfu XIE ; Peijun HUANG ; Zhongjian ZHAO ; Dan CHEN ; Bingfeng ZHANG ; Shiyang PAN
Journal of Modern Laboratory Medicine 2016;(1):25-27
Objective To quantifying the urine human cytomegalovirus(HCMV)DNA from the HCMV infection infants and its corresponding liver function indications,and investigate the relationship between their concentrations.Methods The u-rine samples were collected from HCMV infection infants.HCMV DNA was measured by fluorescence quantitative polymer-ase chain reaction (FQ-PCR).Serum ALT,AST,ALP,GGT,T-Bil and D-Bil liver function indications were detected and the positive rate was analyzed,simultaneously.The correlation between the logarithm urine HCMV DNA (log HCMV DNA) concentration and ALT,AST,ALP,GGT,T-Bil and D-Bil were analyzed by Spearman correlation analysis.Results The dis-tribution range ofurine log HCMV DNA in 444 HCMV infection infants was <2.70~7.90;the positive rate of serum ALT, AST,ALP,GGT,T-Bil and D-Bil were 24.8%,59.0%,95.7%,31.1%,16.7% and 16.3%,respectively.The urine log HC-MV DNA was associated with GGT and the correlation coefficient was 0.099 (P < 0.05),but no associated with ALT, AST,ALP,T-Bil and D-Bil.Conclusion The positive rate of liver function indications will rise in HCMV infection infants, the urine log HCMV DNA was associated with GGT,but not associated with other liver function indications.
7.Clinical efficacy and safety of Biyuan Tongqiao granule combined with triamcinolone acetonide nasal spray in the treatment of chronic rhinosinusitis
Liangru HE ; Jun LIU ; Bingfeng HUANG ; Wei CHEN
Chinese Journal of Primary Medicine and Pharmacy 2018;25(12):1584-1588
Objective To observe the clinical efficacy and safety of Biyuan Tongqiao granule combined with triamcinolone acetonide spray in the treatment of patients with chronic rhinosinusitis .Methods A total of 106 patients with chronic rhinosinusitis in the Traditional Chinese Medicine Hospital of Anji County from February 2015 to February 2017 were selected as study objects .According to different treatment plan , the patients were randomly divided into the control group and the combined group , with 53 cases in each group .The control group received triamcinolone acetonide nasal spray 220μg, 1 time/d, one week later , the dosage adjusted to 110μg, 1 time/d.The combined group was given Biyuan Tongqiao granules 15 g,three times /d on the basis of the treatment of the control group.The clinical efficacy,SONT -20,VAS score,paranasal sinus CT score and incidence rate of adverse reaction were analyzed before and after treatment in the two groups .Results The total effective rate of the combined group was 94.34%,which was higher than 81.13% of the control group,the difference was statistically significant (χ2 =4.296,P=0.038).The SNOT-20,VAS and Lund-Mackey sinus CT score in the combined group were better than those in the control group,the differences were statistically significant (t=7.661,9.189,7.183, 9.269,10.470,all P<0.05).The incidence rate of adverse reactions in the two groups was 7.55%,the difference was not statistically significant (χ2 =0.000,P =1.000).Conclusion Biyuan Tongqiao granule combined with triamcinolone acetonide spray in the treatment of patients with chronic rhinosinusitis can improve the clinical efficacy and reduce pain of patients ,it is worthy of popularization and promotion in clinical .
8. The value of the spondyloarthritis research consortium of ceanada magnetic resonance imaging sacr-oiliac joint inflammation score and structural score in evaluating the activity of axial spondyloarthritis
Zhijian HUANG ; Cundong MI ; Yu DU ; Bo LIU ; Jianyao LIAO ; Ming LIU ; Binbin LIANG ; Yanlin DENG ; Bingfeng LU ; Yi DAI
Chinese Journal of Rheumatology 2019;23(9):612-616,插1
Objective:
To analyze the correlation between the Spondyloarthritis Research Consortium of Canada (SPARCC) magnetic resonance imaging (MRI) sacroiliac joint inflammation score (SPARCC score)/structural score (SSS) and the disease activity as well as the functional indexs. The correlation between the MRI score and inflammatory indicators [erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP)] in patients with active axial spondyloarthritis (axSpA) before and after treatment was explored. In addition, the contribution of the two MRI scoring method in evaluating conditions was also explored.
Methods:
According to the inclusion criteria, 24 patients with active axial SpA were recruited and received the recombinant hauman tumor necrosis factor (TNF)-α receptor Ⅱ: IgG Fc fusion protein(rhTNFR:Fc), sulfasalazine and thalidomide for 12 weeks. Subjects were scored at week 0 and 12 by SPARCC/SSS scores. Bath ankylosing spondylitis disease activity index (BASDAI), Assessment of Spondyloarthritis Intemational Society (ASAS)-endorsed disease activity score(ASDAS)-CRP, bath ankylosing spondylitis functional index (BASFI). Bath ankylosing spondylitis metrology index(BASMI), ESR and CRP. The correlation between the SPARCC/SSS scores and that of clinical indicators were analyzed. Paired sample