Objective To investigate the cause and clinical characteristics of neonatal thrombocytopenia (NT).Methods A retrospective analysis was performed.71 cases with NT were selected as our subjects.The morbidity of NT was calculated.Results Among the 71 cases with NT,34 were term infants and 37 were preterm infants.The morbidity of NT in preterm infants was significantly higher than that in term infants (x2 =24.56,P ＜0.01).Common causes for NT included intrauterine growth restriction(IUGR),severe asphyxia,severe sepsis or necrotizing enterocolitis (NEC).IUGR was the more frequent cause for early-onset thrombocytopenia than for late-onset thrombocytopenia (x2 =9.20,P =0.002).Severe sepsis or NEC was the more frequent cause for late-onset thrombocytopenia than for early-onset thrombocytopenia (x2 =13.58,P ＜ 0.01).The cases of bleeding in mild thrombocytopenia group was less than that in severe thrombocytopenia group(x2 =5.85,P =0.016).Conclusion IUGR which is the common cause for NT frequently induces earlyonset thrombocytopenia.Severe sepsis or NEC frequently induces late-onset thrombocytopenia accompanied with high bleeding risk.Earlier diagnosis of the cause for NT and tailed treatments can achieve better efficacy.

ObjectiveTo study the feasibility,efficacy and safety of u tilizing the laryngeal mask airway (LMA) ventilation compared with the endotracheal intubation ( ET ) in neonatal resuscitation for moderate and severe asphyxiated neonates.MethodsNeonates requiring positive pressure ventilation with heartrate ＜60 beats/min were collected and grouped quasi-randomizedly into LMA(36 cases) or ET(32 cases)ventilation.Differences of resuscitation effect,inserting time,successful once insertion rate and adverse reactions between the two groups were observed and compared.Results( 1 ) No significant difference was observed in Apgar scores at 1 min and 5 min between the two groups ( P＞0.05 ).(2) Success rate of once insertion was 94.4％ with average inserting time ( 7.58±1.16 ) s for LMP group,while it was 90.6％ and ( 7.89 ± 1.52) s for ET group.( 3 ) Successful resuscitation rate of LMA group ( 86.11％ ) was slightly lower than ET group (96.88％ ),but there was no statistical difference (P＞0.05).(4) Mean response time of LMA group [ (34.06 ± 10.56) s] was slightly lower than that of ET group [ (41.38 ±27.19) s],also ventilation time of LMA group [( 137.19 ±80.14) s] was slightly lower than that of ET group [ ( 171.09±84.28 ) s ],but neither showed statistical difference ( P＞0.05 ).(5) Adverse reactions were found in LMA group including nausea( 2 cases )and abdominal distention (1 cases),while there were laryngeal edema( 1 cases),pneumothorax(2 cases),respiratory tract bleeding( 1 cases) in ET group.ConclusionThe LMA ventilation is much easier to operate,with its effect no less than that of ET ventilation on resuscitation for moderate and severe asphyxiated cases,even it seems more safe.LMA ventilation can be a good substitute for ET ventilation,especially for those medical staffs who are unfamiliar with ET operation and primary hospital doctors in case of emergency.

Cai which are stem or heartwood of trees or stem of woody liana are a kind of medicinal plant resources traditionally used by Li Minoriy. Ethnobotanical methods were adopted for the investigation on traditional utilization of cai. Totally, 44 species in 26 families and 30 genera were reported to be used as cai. Most of the species were applied as decoction (70.5%) and medicinal liquor (27.3%) to treat a wide range of diseases, such as rheuma-toid arthritis (34.1%), liver diseases (18.2%), injuries and weak (15.9% respectively). Through literature survey, it was found that 7 species (15.9%) were used by Li Minority only. Besides, 13 species (29.5%) were used differently compared with that of Traditional Chinese Medicine. Consequently, it is indicated that indigenous knowledge of using medicinal plants by Li Minority is unique.

Objective To analyze the incidence of retinopathy of prematurity(ROP)among extreme-ly preterm infants,and to evaluate the treatment methods and effects among those with severe ROP.Methods A retrospective analysis was performed to analyze incidence of ROP in 96 cases of extremely preterm infants who were born at a gestational age of 〈28 weeks and survived beyond a postmenstrual age of at least 1 year from Apr 2006 to Oct 2013,and to analyze the treatment outcomes of photocoagulation and ranibizum-ab intravitreal injection among the infants with severe ROP.Results Fifty-six of 96 cases(58.33%)grew into ROP finally and 21 cases(21.88%)grew into severe ROP,2 cases(2.08%)grew into aggressive poste-rior ROP.Fifteen cases with severe ROP were treated with laser photocoagulation.Four cases with severe ROP were received ranibizumab intravitreal injection prior to photocoagulation.Two cases with severe ROP were only treated with ranibizumab intravitreal injection.The eyesight of 96 patients (100%)in this study were all preserved.Conclusion ROP screening should focus on extremely preterm infants because of higher incidence of ROP and severe ROP among them.The infants with severe ROP should be treated with laser photocoagulation in time.The infants in critical condition or with aggressive posterior ROP can be treated with ranibizumab injection.

Medical implantable stainless steels are widely used in medical field due to their excellent properties, besides its allergic response to human body, the nickel ion released from the steels due to corrosion has the harm of malformation and carcingenesis. The mechanical property, corrosion resistance and blood compatibility of a new nickel-free stainless steel (BIOSSN4) is researched in this paper. Compared with the traditional 316L medical stainless steel, BIOSSN4 shows wide future applications because of its better combination of strength and toughness, good corrosion resistance and biocompatibility.
Biocompatible Materials ; chemistry ; toxicity ; Corrosion ; Humans ; In Vitro Techniques ; Materials Testing ; Nickel ; chemistry ; toxicity ; Platelet Adhesiveness ; drug effects ; Stainless Steel ; chemistry ; toxicity ; Surface Properties

OBJECTIVETo investigate the photosynthetic characteristics of wildlife tending Alpinia oxyphylla, and provide a theoretical basis for choosing wildlife tending environment and cultivation management.

METHODThe response parameters of the net photosynthetic rate to light intensity, CO2 concentration and photosynthetic characteristics were measured by Li-6400 portable photosynthesis in blossom bud forming stages under different treated conditions.

RESULTThe maximum net photosynthetic rate (Pmax), daily average photosynthetic rate (Pn), apparent quantum efficiency (AQY), apparent carboxylation efficiency (CE), light using efficiency (LUE), and water use efficiency (WUE) were optimal in the wild tending treatment at the light transmission rate of 17.4%-24.1%, beyond the light transmission rate, the photosynthetic capacity utilization of A. oxyphylla would not have a significant increase or be inhibited. The light compensation point (LCP) and light saturation point (LSP) of A. oxyphylla improved with light intensity enhancing. Wildlife tending could enhance the scope of A. oxyphylla to CO2 adaptation.

CONCLUSIONA. oxyphylla as sciophytes, and the optimum light transmission rate for wild tending and cultivating was at 17.4%-24.1%.

Alpinia ; metabolism ; radiation effects ; Carbon Dioxide ; metabolism ; Light ; Photosynthesis ; radiation effects ; Plant Leaves ; metabolism ; radiation effects

BACKGROUND: Magnalium which is potential to be the medical biodegraded metal implant is more and more interesting,but it must be well biocompatibility to human body.OBJECTIVE: To evaluate the sensitization of magnalium (AZ31B).METHODS: A total of 35 guinea pigs were randomly divided into saline group (negative control group,n=10),5% volume of formaldehyde (positive control group,n=10),and AZ31B group (n=15).Sensitization test at the maximal dosage was performed according to "Biological evaluation of medical devices-Part 10: Tests for irritation and delayed-type hypersensitivity",including intracutaneous induction,local induction,and provocation.Patch was removed after 6,24,48,and 72 hours,and the skin response was classified accordingto Magnusson and Kligman criteria.Patch was removed after 72 hours,and skin was performed with biopsy,stained with FIE staining,and observed under optic microscope.RESULTS AND CONCLUSION: Sensitization response was not tested in both negative control group and AZ31B group at 24,48,and 72 hours after patch removal; however,moderate erythema was observed in the positive control group.Optic microscope demonstrated that criteria of allergy such as spongiosis,edema,and diffuse as well as perivascular mononuclear infiltration was not observed in the AZ31B group,but a few basophilic calls ware observed.This suggested that AZ31B was biologically safe for sensitization.

OBJECTIVETo investigate STK11 gene mutation in a pedigree with Peutz-Jeghers syndrome (PJS).

METHODSA pedigree of PJS was investigated. DNA was extracted from peripheral blood samples from affected and unaffected members of the pedigree and 100 unrelated healthy controls. PCR was performed to amplify all of the 9 coding exons of STK11 gene. PCR products were directly sequenced to detect mutation.

RESULTSA missense mutation p.F354L (c.1062C>G) in exon 8 of the STK11 gene has been identified in all patients with PJS, but was not found in normal individuals from the pedigree and 100 unrelated controls.

CONCLUSIONA missense mutation p.F354L of STK11 gene probably underlies the disease in this pedigree.

Adult ; Asian Continental Ancestry Group ; genetics ; Base Sequence ; DNA Mutational Analysis ; Exons ; Female ; Humans ; Male ; Molecular Sequence Data ; Pedigree ; Peutz-Jeghers Syndrome ; diagnosis ; enzymology ; genetics ; Protein-Serine-Threonine Kinases ; genetics

The objective of this experimental study was to assess the effects of a new kind of drug eluting coronary stent. Fourteen mini-pigs were used; seven normal stainless stents and seven new drug eluting stents were implanted in their normal coronary arteries, respectively. Angiography was performed and followed by pressure-fixation of the coronary arteries for light and electron microscopic examinations at the end of three months after implantation. Repeated angiography showed that all the stented coronary segments were open. With no additional antithrombotic treatment, there was no thrombus formed in the stented coronary segments. Scanning electron microscopy analysis showed the implanted stent surface was covered by endomembrane without thrombus formation. The endothelial cell in the membrane was clear and lined by the direction of blood flow. Histomorphological analysis revealed the neointima in normal stainless stent group was thicker than that in new drug eluting stent group, and the neointima was composed of smooth muscle cell and extracellular matrix. The result of this study shows that this kind of stent could reduce the rate of the re-stenosis and occlusion of PTCA. This stent can be used in clinical trials.
Angioplasty, Balloon, Coronary ; instrumentation ; Animals ; Coated Materials, Biocompatible ; chemistry ; Coronary Restenosis ; prevention & control ; Drug-Eluting Stents ; Random Allocation ; Swine ; Swine, Miniature ; Titanium ; chemistry

Objective:To study the incidence and risk factors of periventricular- intraventricular hemorrhage (PIVH) in extremely preterm infants (EPI) with gestational age (GA)<28 weeks.Methods:A retrospective study was performed in 304 cases of EPI hospitalized between January 2016 and December 2018. The infants were assigned into two groups according to whether PIVH occurred. Univariate analysis and Logistic regression analysis were used to determine the risk factors of PIVH.Results:Among the 304 cases,101 (33.2%) developed PIVH and 44 (14.5%) developed severe PIVH.The incidences of PIVH and severe PIVH in EPI with birth weight (BW) <750 g were 50.6% and 31.0%. The incidences of PIVH and severe PIVH in EPI with GA<26 weeks were 51.4% and 27.5%. Logistic regression analysis revealed that advanced GA ( OR=0.697, 95% CI 0.543~0.895, P=0.005) decreased the risk of PIVH. Prolonged invasive mechanical ventilation ( OR=1.121, 95% CI 1.007~1.249, P=0.037) and use of vasoactive drugs ( OR=1.373, 95% CI 1.040~1.812, P=0.025) within the first week of life increased the risk of PIVH. Conclusions:The incidences of PIVH and severe PIVH in EPI are quite high. Smaller GA, longer use of invasive mechanical ventilation and vasoactive drugs within the first week will increase the risk of PIVH in EPI.