Emotional illness spread in every clinical system disease, contained very complex sub-illness. At present, though there were plenty of clinical reports on emotional illness, few reports were substantive. Therefore, this paper put forward to establish a standard emotional diagnosis system by inheriting the traditional four diagnostic methods and absorbing the diagnostic techniques of modern psychosomatic medicine;according to the specific circumstances of emotional illness, the individual simultaneous treatment or separate treatment of mind and physique was used to establish a standardized system for treating emotional illness

Objective To observe changes of the expression of Bcl‐2 and endothelial nitric oxide synthase (eNOS) in lung tissue ,which was used the indifferent treatment doses of recombinant human erythropoietin (rhEPO) of newborn SD model of a new type of bronchial pulmonary dysplasia .To explore its effect on the protection of the new type of bronchial pulmonary dysplasia (BPD) ,and find out the appropriate drug delivery time and dosage .Methods We used the things lipopolysaccharide (LPS) and continuous high oxygen preparation to make the new model of the BPD .We made random normal newborn mice 18 as normal air group ,and then 90 newborn baby mice model of random points normal saline group (group A) ,rhEPO800 group (group B);rhEPO 1000 group (group C) ,rhEPO1200 group (group D) ,rhEPO1400 group (group E) ,in experiment 1 ,7 ,and 14 days ,we were ran‐domly selected 6 executed only lung tissue under liquid nitrogen .Using RT‐PCR and Western blot method to detect the Bcl‐2 and eNOS protein and mRNA expression in lung tissue .Results Compared with the normal air group ,the expression of protein and mRNA of Bcl‐2 in group A was decreased ,while the expression of eNOS was increased .Compared with group B ,C ,D ,E ,with the increase of rhEPO concentration ,the expression of Bcl‐2 protein and mRNA in group A gradually increased ,while the expression of eNOS gradually decreased ,and the expression of the group E was the most obvious ,and the difference was statistically significant (P<0 .05) .Conclusion It is prompted that rhEPO has some treatment function to the new BPD ,while the dose of 1 400 IU/kg of 14 days have the best function .

Objective To explore the effect of Buzhong Yiqi combined with low frequency impact feedback and rehabilitation training on postpartum pelvic floor rehabilitation.Methods100 cases of maternal fertility were randomly divided into observation group and control group, and 50 cases were randomly divided into observation group and control group.The control group was given low-frequency shock and electrical stimulation and rehabilitation training intervention, the observation group in the control group at the same time plus the addition of Buzhong Yiqi Tang treatment, were treated for 3 months.To evaluate and compare the pelvic floor muscle function, vaginal tension, vaginal pressure and nocturia before and after treatment, and compare the quality of life and the occurrence of complications after treatment.ResultsThere were no significant differences in the parameters of the pelvic floor muscle, vaginal tension, vaginal pressure and nocturia before treatment.and the maximal muscle voltage of the pelvic floor muscle and the pelvic floor muscle contraction were 60 s, the mean muscle voltage values of the pelvic floor muscle were significantly increased (P<0.05).However, the vaginal muscle tension and vaginal pressure in the observation group were significantly higher than those in the control group (P<0.05), and the vaginal muscle tension and vaginal pressure were significantly higher (P<0.05), but the number of nocturia in the observation group was significantly lower than that in the control group (P<0.05), and the FS-FI score of the observation group was significantly higher than that of the control group (P<0.05).The incidence of urinary incontinence was 4.26% and 6.38% in the observation group were significantly lower than those in the control group (16.67%, 18.75%, P<0.05).ConclusionBuzhong Yiqi Fang combined with low-frequency shock and electric stimulation, rehabilitation training treatment is conducive to promoting maternal postpartum pelvic floor rehabilitation, improve sexual quality of life, and can effectively reduce the incidence of pelvic organ prolapse, urinary incontinence.

Endogenous hydrogen sulfide (H_2S) is to third found gaseous transmitter.In cardiovascular system, H_2S is produced endogenously by cystathionine γ-lyase (CSE)and plays an important role in the regulation of cardiovascular physiologic function and patho physiological processes.The CSE/H_2S system has a variety of biological effects in the cardiovascular system, such as inducing vasorelaxation,decreases blood pressure,inhibiting vascular smooth muscle cell proliferation and inducing anti-oxidant.It is also concerned with variety of cardiovascular diseases such as hypertension, hyperlipemia, atherosclerosis,coronary artery disease and so on.We summarize the biochemical characteristics and the physiological functions of endogenous H_2S and its patho physiological effects in cardiovascular diseases.

Objective To analyze the detection results of 75 suspected type A influenza cases and to understand the distribution situation of influenza patients to provide the guidance for its early prevention and control .Methods The nasopharyngeal swabs specimens from 75 patients with suspected type A influenza were detected for understanding the viral infection situation by real‐time fluorescent quantitative PCR .Results In the specimens of 75 suspected patients ,13 cases with type A influenza virus were detected out ,in which 9 cases were the H1N1 subtype ,mean age > 50 years old .The temporal distribution was 3 cases in March 2013 ,1 case in April ,7 cases in January 2014 and 2 cases in February .No H7N9 subtype was detected out .Conclusion Real‐time fluores‐cent quantitative PCR has the characteristics of rapidness and high accuracy .The winter is the high onset season of flu ,elderly pa‐tients are susceptible to influenza virus .The monitoring and early treatment should be strengthened .

Blood stasis is the main pathological factor in the occurrence of cardiovascular diseases. Notoginseng Radix et Rhizoma is the representative medicine for activating blood and dissipating stasis. The effective components of panax notoginseng saponins, the active part of Notoginseng Radix et Rhizoma, are ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rd, and notoginsennoside R1, having good intervention effects on cardiovascular diseases, which have become the research hotspots in recent years. This article reviewed the research progress in Notoginseng Radix et Rhizoma and its major saponins in the field of cardiovascular diseases, and provided ideas for clinical prevention and treatment and research and development of medicine.

OBJECTIVE:To observe the clinical efficacy and safety of Aidi injection combined with TP regimen in the treat-ment of non-small cell lung cancer. METHODS:62 patients with non-small cell lung cancer were randomly divided into control group and observation group,31 cases in each group. Control group received TP regimen alone,observation group additionally re-ceived 40 ml Aidi injection,dissolved in 500 ml 0.9% Sodium chloride injection by intravenous infusion. 21 d was a treatment course,and it lasted 4 courses. The level of inflammatory cytokines before and after treatment and post-treatment quality of life, short-term efficacy,patients’satisfaction and toxicity reactions in 2 groups were compared. RESULTS:After treatment,the level of inflammatory cytokines in 2 groups were significantly lower than before,and observation group was lower than control group, the differences were statistically significant (P<0.05). The effective rate of life quality (80.64%) and short-term efficacy (83.87%)in observation group significant were higher than control group(32.25%,64.52%,respectively),the differences were statistically significant (P<0.05). Satisfaction degree (96.77%) in observation group was significantly higher than control group (77.42%);and the incidences of thrombocytopenia,leukopenia,abnormal liver function were significantly lower than control group,the differences were statistically significant (P<0.05). CONCLUSIONS:Aidi injection combined with TP regimen shows good efficacy and little toxicity in the treatment of non-small cell lung cancer,and it helps to reduce the level of inflammatory cyto-kines in patients,improving immune function and the quality of life of patients.

Objective To observe the change of early postoperative cognition in the elderly under low central venous pressure (LCVP) after lobe resection to investigate the safety and feasibility of LCVP in Geriatrics.Methods A total of 45 ASA class Ⅰ ～ Ⅱ old patients (60 ～ 75 y)with elective liver resection were divided into L group and C group.In the LCVP group (L group),CVP was maintained below 5 cmH2O during liver resection until the lobe was done.The patients in the control group (C group) received standard care (The CVP was controlled between 6 cmH2O to 12 cmH2O).To compare the anesthesia recovery after surgery,all patients were tested with a battery of neuropsychologic assessment of cognitive function preoperatively and on the 7th day postoperatively.Results During liver resection,the MAP [(75.8 ±7.9)mmHg] and CVP [(3.1 ±0.4)cmH2O] of experimental group were lower than the control group [MAP (92.3 ± 10.6)mmHg,CVP(9.3 ± 1.4)cmH2O].The difference was statistically significant (t' =20.08,P ＜0.05,t =5.89,P ＜0.01) ;There was no statistically significant difference in postoperative recovery of spontaneous breathing,respiratory,eye opening time,extubation time and leave the operating room time between two groups[(18.1 ±6.7)min,(25.4±8.3)min,(31.9±11.7)min,(42.8±17.8)minvs (15.3 ± 7.0)min,(22.6 ±9.4)min,(30.2 ± 10.8) min,(45.4 ± 13.6) min,P ＞ 0.05].The incidence of POCD was 30.0％ in the experimental group and 27.3％ in the control group.The difference was no statistical significance between two groups(P ＞0.05).Conclusious There was no significant influence of low central venous pressure on anesthesia recovery time and early postoperative cognition in the elderly under hepatic resections.

BACKGROUND:Decreased function and reduced number of CD4+CD25+regulatory T cells have been considered the major manifestation of immunity dysfunction in children with primary nephrotic syndrome. Bone marrow mesenchymal stem cells have immunoregulation effects, which up-regulate CD4+CD25+regulatory T cells, inhibit proliferation of lymphocytes, and have been widely used in many immune diseases.
OBJECTIVE:To investigate the effects of bone marrow mesenchymal stem celltransplantation on the CD4+CD25+regulatory T cells of peripheral blood in rats with primary nephrotic syndrome.
METHODS:Bone marrow mesenchymal stem cells from six Sprague-Dawley rats were isolated, passaged and utilized for cellsuspension preparation. At the third passage, bone marrow mesenchymal stem cells were used for transplantation. The remaining 30 rats were randomly and equal y divided into three groups:normal group, normal saline infusion group, and bone marrow mesenchymal stem cells group. The rat models of primary nephrotic syndrome were established by single injection of adriamycin intravenously through tail vein in the latter two groups. Rats were then treated with bone marrow mesenchymal stem cells (1×10 7 ) (bone marrow mesenchymal stem cells group) or normal saline (normal saline infusion group) through tail vein at the same time after adriamycin administration. The normal group received no treatment.
RESULTS AND CONCLUSION:Compared with the normal group, rats in the normal saline infusion group developed nephropathy characterized by ascites, proteinuria, hypoalbuminemia, hypercholastero-lnemia, and progressive renal injury. However, the proteinurine and clinical severity in bone marrow mesenchymal stem cells group were significantly ameliorated after treatment with bone marrow mesenchymal stem cells. CD4+CD25+Treg/CD4+Treg in the peripheral blood in the bone marrow mesenchymal stem cells group and normal saline infusion group were significantly higher than that in the normal group at 28 days after model establishment (P<0.05), while there was no significant difference between bone marrow mesenchymal stem cells group and normal saline infusion group (P>0.05). The expression of FoxP3 mRNA in the peripheral blood mononuclear cells of the bone marrow mesenchymal stem cells group was significantly higher than that in the normal saline infusion group and normal group (P<0.05). The bone marrow mesenchymal stem cells play a protective effect in rats with primary nephrotic syndrome, which may be related to the increase of local expression of FoxP3 and generation of CD4+CD25+Treg.

BACKGROUND: Under specially physical environment, for example weightlessness, metabolism of bone tissue may have remarkable changes; however, osteoblast is a core of bone metabolism and bone formation, so it is very sensitive to changes of gravity environment.OBJECTIVE: To observe the effects of simulated weightlessness on bone marrow stromal cell count and osteogentic capacity of weight bearing bone in rats so as to reveal the mechanism of bone loss.DESIGN : Randomized pairing and controlled study.SETTING: College of Aerospace Medicine and Department of Pathology of Stomatology College, the Fourth Military Medical University of Chinese PLA.MATERIALS: A total of 20 adult healthy male SD rats were selected in this study. At the beginning of experiment, rats based on their body mass were randomly divided into control group and suspension group with 10 in each group. Alkaline phosphatase kit was provided by Beijing Zhongsheng Bioengineering High-technological Company.METHODS: The experiment was carried out in the Department of Pathology, Collage of Stomatology, the Fourth Military Medical University of Chinese PLA from November 1999 to July 2000. Rats were randomly divided into tail suspension group and control group with 10 in each group. Rats in the tail suspension group were given tail suspension for 28 days. Their heads maintained 30° low position, and their hindlimbs freely suspended and were not given weight loading. While, rats in the control group were fed normally. At the end of experiment, bone marrow stromal cells were obtained from femur for primary and transferring cultures.MAIN OUTCOME MEASURES: Cell counting and methylthianolyldiphenyl-tetrazolium bromide (MTT) assay were used to draw growth curve of cells in primary and transferring cultures and to measure activity of alkaline phosphatase and forming quantity of mineralized nodules in vitro.RESULTS : ① Activity of alkaline phosphatase: Activity of alkaline phosphatase of cells in the primary and transferring cultures in the suspension group was lower than that in the control group, and there was significant difference between them (P＜0.05). ② Forming quantity of mineralized nodules: Forming quantity of mineralized nodules in the suspension group was less than that in the control group, and there was significant difference between them (P＜0.05). ③ Cell growth: Growth curve of femoral stromal cells in primary and transferring culture manifested as S. Doubling time of cells in the suspension group was similar to that in the control group. ④ Amount of bone marrow stromal cells in femur: Amount of bone marrow stromal cells in primary culture in the suspension group was decreased as 50% as that in the control group (P＜0.05).CONCLUSION: Under simulated weightlessness, amount of bone marrow stromal cells decreases obviously; in addition, amount of osteoblast also decreases in weight bearing bone of hindlimbs and osteogentic capacity in vitro decreases simultaneously.