Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Molar ; Root Canal Therapy ; methods

The isolated 24 strains-producing hydantoinase & carbamoylase were first identified by Biolog microbial identification system and 16S rDNA sequence analysis.The results suggested that the hydantoinase & carbamoyalse-producing bacteria belonged to Bacillus,Geobacillus,Brevibacillus,Aneurinibacillus,Microbacterium,Pseudomonas,Kurthia and Empedobacter,and so on.Especially,Kurthia and Empedobacter were new hydantoinase & carbamoylase-producing genera.Furthuremore,it was found that D-hydatoinase & carbamoyalse-producing bacteria belonged to Pseudomonas and Agrobacterium,while most of L-hydantoinase & carbamoyalse-producing bacterial belonged to Bacillus,Geobacillus and Microbacterium.The distribution feature of D-hydantoinase & carbamoyalse-producing bacteria and L-hydantoinase & carbamoyalse-producing bacteria showed some genera tendency.This research work will provide the biomaterial of different hydantoinase and carbamoylase and contribute to study the structure and function,molecular evolution of the two enzymes.

OBJECTIVETo investigate the effect of unaggregated Abeta(25.35) on delayed rectifier potassium current (I(K)) in neonatal rat hippocampal CA3 pyramidal neurons.

METHODSThe rat hippocampal neurons were enzymatically isolated from 10-11-day-old Wistar rat. The I(K) was recorded using whole-cell patch clamp technique.

RESULTSThe inhibitory effect of unaggregated Abeta(25-35) on I(K) was time-dependent, because I(K) significantly decreased from (6.987 +/- 1.152) nA to (2.540 +/- 0.349) nA after adding unaggregated Abeta(25-35) and reached a stabilized level after 5-7 min (n = 8, P <0.01). However, the inhibitory effect was not concentration-dependent, because the decrease of the I(K) amplitude in different concentration groups were all around 60%. Unaggregated Abeta(25-35) also remarkably affected the half-activation potential, which was (4.114 +/- 0.730) mV and (-5.463 +/- 0.950) mV before and after its application (n = 15, P <0.05); however, the slope factor of activation curve was not significantly changed.

CONCLUSIONThe inhibitory effect of unaggregated Abeta(25-35) on I(K) may be a possible mechanism involved in the pathogenesis of Alzheimer's disease.

Alzheimer Disease ; metabolism ; Amyloid beta-Peptides ; chemistry ; pharmacology ; Animals ; Animals, Newborn ; Cells, Cultured ; Female ; Hippocampus ; cytology ; Male ; Mice ; Neurons ; drug effects ; metabolism ; Patch-Clamp Techniques ; Potassium ; metabolism ; Rats ; Rats, Wistar

OBJECTIVETo investigate different angle projection technique for the clinical detection and treatment of multiple canals in mandibular anterior teeth and premolars.

METHODSTwo hundred and forty-seven in vivo mandibular anterior teeth and premolars were selected from two hundred and fourteen patients. Four kinds of radiographs were taken for each tooth. The radiograph was taken at a horizontal angles of 0, 20 - 30 degrees from the mesial or distal of the tooth with and without files in canal. If a radiolucent line or files was present mesial or distal to the main canal, an additional canal was suspected. If the tooth appeared to have one large canal in the cervical or middle third of the root which disappeared or constricted as it traveled in an apical direction, an additional canal was suspected. The root canals were instrumented with ProTaper in crown-down mode and filled with laterally condensed gutta-percha and paste, the root canal configurations were classified into Types I - V.

RESULTS60.92% multiple canals and 26.44% long oval canals were detected and treated from suspected multiple canals. The sensitivity of angle projection technique with file in X-ray diagnosing of multiple canals was 93.0%, and second canal was missed in four cases. The multiple canals in the 247 mandibular anterior teeth and premolars were present in central incisors: 9.43% (5 of 53); lateral incisors: 38.33% (23 of 60); canines: 15.90% (7 of 44), first premolar: 40.38% (21 of 52); second premolar: 2.63% (1 of 38).

CONCLUSIONSThe different angle projection technique will assist the clinician in the detection and treatment of multiple canals in mandibular anterior teeth and premolar, and angle projection technique with file detected more multiple canals.

Adult ; Aged ; Aged, 80 and over ; Bicuspid ; diagnostic imaging ; Dental Pulp Cavity ; anatomy & histology ; diagnostic imaging ; Female ; Humans ; Incisor ; diagnostic imaging ; Male ; Mandible ; diagnostic imaging ; Middle Aged ; Radiography ; Root Canal Therapy

Objective: To observe the functional changes of high density lipoprotein (HDL) subclass in patients with aortic dissection and to explore its clinical significance. Methods: Our research included in 2 groups: Aortic dissection group, n=60 patients and Control group, n=60 healthy subjects from regular physical examination. Blood levels of lipids, HDL subclass of HDL2 and HDL3, macrophage [3H] cholesterol efflux rate, intercellular adhesion molecule-1(ICAM-1), intravascular cellular adhesion molecule-1 (IVCAM-1) and paraoxonase 1 (PON-1) activity were examined and compared between 2 groups. Results: Compared with Control group, Aortic dissection group had reduced HDL2 level (41.15±19.02) mmol/L vs (60.48±22.19) mmol/L, P=0.010 and elevated HDL3 level (58.85±29.97) mmol/L vs (39.52±30.15) mmol/L, P=0.013;decreased macrophage [3H] cholesterol efflux rate (5.79±0.97) ％ vs (11.45±2.15) ％, P<0.01 and decreased PON-l activity (125.62±9.90) μ/ml vs (158.72±13.27) μ/ml, P<0.01; increased expressions of ICAM-1 (33571.85±2529.97) vs (17239.52±4330.15) and IVCAM-1 (442.15±119.02) vs (260.48±92.19) by fluorescence intensity, both P<0.05. Conclusion: Aortic dissection patients showed damaged HDL subclass reverse cholesterol transportation function and anti-oxidative function, both of them were lower than healthy population.

Objective To investigate the CT findings of Madelung's disease in the head and neck region,and to evaluate the value of CT in demonstrating the Madelung's disease in the head and neck region.Methods CT findings of Madelung's disease in the head and neck region in 7 cases were analyzed retrospectively.All were males,with the age from 36 to 60 years,mean 51 years.All patients were underwent CT native scan,and enhanced CT scan was performed on 3 of them.Results CT images in the neck of all patients showed accumulation of nonencapsulated fat within the subcutaneous tissue and(or) deep to the platysma,and(or)within the spaces between the muscles.The fat deposits were ill-defined and symmetrical.In most cases the fat deposits involved the anterior part of the neck(infrahyoid and suprahyoid),submandibular region,the subcutaneous tissue of the nape and deep to the stenomastoid muscles.Conclusions Madelung's disease in the head and neck region have characteristic CT findings,and CT has great value in qualitative and quantitative diagnosis in Madelung's disease.

BACKGROUNDAdipose tissue-derived stromal cells (ADSCs) can be greatly expanded in vitro, and induced to differentiate into multiple mesenchymal cell types, including osteogenic, chondrogenic, myogenic, and adipogenic cells. This study was designed to investigate the possibility of ADSCs differentiating into neurons.

METHODSAdipose tissue from rats was digested with collagenase, and adherent stromal cells were cultured. A medium containing a low concentration of fetal bovine serum was adopted to induce the cells to differentiate. ADSCs were identified by immunocytochemistry, and semi-quantitative RT-PCR was applied to detect mRNA expression of neurofilament 1 (NF1), nestin, and neuron-specific enolase (NSE).

RESULTSNestin-positive cells were found occasionally among ADSCs. ADSCs were found to express NSE mRNA and nestin mRNA, but not NF1 mRNA. ADSCs could differentiate into neuron-like cells in a medium composed of a low concentration of fetal bovine serum, and these differentiated cells displayed complicated neuron-like morphologies.

CONCLUSIONSThe data support the hypothesis that adipose tissue contains stem cells capable of differentiating into neurons. These stem cells can overcome their mesenchymal commitment, and may represent an alternative autologous stem cell source for CNS cell transplantation.

Adipose Tissue ; cytology ; Animals ; Cell Differentiation ; physiology ; Cells, Cultured ; Immunohistochemistry ; Intermediate Filament Proteins ; analysis ; Nerve Tissue Proteins ; Nestin ; Neurofilament Proteins ; analysis ; Neurons ; cytology ; Phenotype ; Phosphopyruvate Hydratase ; analysis ; Rats

OBJECTIVETo investigate the efficiency of different angle projection radiograph in diagnosing multiple canals of mandibular first premolars.

METHODSEighty-eight mandibular first premolars that needed endodontic treatment in vivo were selected. The radiograph was taken at a horizontal angles of 0, 20-30 degrees from mesial or distal direction of the tooth preoperative. According to the different angle projection radiographic evaluation, radiographic diagnosis of multiple canals were suspected. After the root canal orifice were detected and located with K files, the root canals were prepared using crown-down technique and obturated using cold lateral condensation technique. The configurations and numbers of root canals were identified and recorded. The postoperative radiograph was taken. According to configurations and postoperative radiograph, the root canal configuration was classified into type I -V.

RESULTSIn eighty-eight mandibular first premolars, 31 multiple canals teeth were suspected in preoperative radiographic diagnosis, 30 multiple canal teeth were detected under clinical evaluation. In case of 31 multiple canal teeth suspected by preoperative radiographic diagnosis, 3 teeth were not found multiple canals under clinical evaluation. While in case of 2 teeth of one canal suspected by preoperative radiographic diagnosis, multiple canal was detected under clinical evaluation. In 31 multiple canal teeth suspected by preoperative radiographic diagnosis, 13 teeth were detected at a horizontal angles of 0 degree and 25 teeth were detected at a horizontal angles of 20-30 degree, 7 teeth were suspected at different angle projection. Clinical detected rate of multiple canal in mandibular first premolar were 34.09% (30/88), 70.00% of which were type IV and V.

CONCLUSIONDifferent angulation radiograph of preoperative will assist to increase clinical detection rate of multiple canals in mandibular first premolar.

Bicuspid ; Dental Pulp Cavity ; Humans ; Incisor ; Mandible ; Maxilla ; Molar ; Postoperative Period ; Root Canal Therapy

OBJECTIVETo investigate individualized one-staged correction of alveolar cleft and lip and nasal deformities secondary to lip cleft.

METHODSThe alveolar cleft and lip and nasal deformities secondary to lip cleft were corrected in one stage.

RESULTSFrom 2004 to 2007, 37 cases were treated. 33 patients were treated successfully with primary healing in bony recipient area. Cancellous bone exposure happened in 3 cases. The wounds healed after debridement and drainage. The cosmetic results were satisfactory.

CONCLUSIONSOne-staged correction of alveolar cleft and the lip and nasal deformities secondary to lip cleft can achieve good results.

Adolescent ; Alveolar Process ; abnormalities ; Child ; Cleft Lip ; complications ; surgery ; Cleft Palate ; complications ; surgery ; Female ; Humans ; Male ; Nose ; abnormalities ; Nose Deformities, Acquired ; surgery