1.Expression change of TIF3 p36 at different stages of human bronchial epithelial cells transformed and induced by cadmium chloride.
Lian WEI ; Yi-xiong LEI ; Min WANG ; Bing HU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(10):578-581
OBJECTIVETo explore the molecular mechanisms potentially responsible for carcinogenesis due to cadmium by detecting expression change of the translation initiation factor 3 (TIF3 p36) in those malignant transformation of human bronchial epithelial cell lines (16HBE) induced by cadmium chloride (CdCl(2)).
METHODSThe expression changes of TIF3 p36 were detected and analyzed at different stages of malignant cells (semi transformed cells, transformed cells and tumorigenic cells) induced by CdCl(2) solution with both reverse transcription PCR technique and sensitive fluorescent quantitative PCR assay.
RESULTSCompared with non-transformed human bronchial epithelial cells, the results of fluorescent quantitative PCR assay showed that the semi-transformed cells, transformed cells and tumorigenic cells all expressed higher levels of TIF3 p36 mRNA (P < 0.01 or P < 0.05). As compared with the control cells, the TIF3 expressions at different stages of malignant transformation were 3.1 times, 5.9 times and 9.9 times higher respectively in the low dosage group of CdCl(2) (5 micromol/L); 7.1 times, 6.8 times and 14.8 times respectively in the middle dosage group of CdCl(2) (10 micromol/L); 3.6 times, 3.0 times and 9.1 times respectively in high of dose of CdCl(2) (15 micromol/L). These results showed that there was the positive correlation between overexpression levels of TIF3 p36 mRNA and the malignant degree of the cells, but they were not related to the dosages of cadmium.
CONCLUSIONThere is significantly abnormal overexpression of TIF3 gene during malignant transformation of human bronchial epithelial cell line induced by cadmium chloride, and the TIF3 expression is associated with the malignant degree of the cells, which may be one of molecular mechanisms potentially responsible for the carcinogenesis due to cadmium.
Bronchi ; cytology ; Cadmium Chloride ; toxicity ; Cell Line ; Cell Line, Transformed ; Cell Transformation, Neoplastic ; chemically induced ; metabolism ; Dose-Response Relationship, Drug ; Humans ; Prokaryotic Initiation Factor-3 ; biosynthesis ; genetics ; RNA, Messenger ; genetics ; Reverse Transcriptase Polymerase Chain Reaction
2.Protective effects of valproic acid on gut barrier function after major burn injury and its mechanism
Hongmin LUO ; Sen HU ; Huining BIAN ; Shaoyi ZHENG ; Bing XIONG ; Zhifeng HUANG ; Zu'an LIU ; Chuanwei SUN ; Lianghua MA ; Hanhua LI ; Wen YU ; Minghua DU ; Huada CHEN ; Wen LAI
Chinese Critical Care Medicine 2017;29(3):221-227
Objective To investigate the potential protective effects of valproic acid (VPA) on gut barrier function after major burn injury in rats and its mechanism.Methods Forty male Sprague-Dawley (SD) rats were divided into sham + normal saline (NS),sham + VPA,scald + NS,and scald + VPA groups,with 10 rats in each group.Rat with 55% total body surface area (TBSA) third-degree severe-bums model was reproduced by immersing into 80 ℃ water,and the rats in sham groups were given sham-bums by immersing into 37 ℃ water.The rats after severebums were immediately treated with 0.25 mL of 300 mg/kg VPA or NS by subcutaneous injection.Rats were sacrificed at 2 hours and 6 hours after injury,and abdominal aortic blood and ileal tissue were harvested.The levels of vascular endothelial growth factor (VEGF) were determined by enzyme-linked immunosorbent assay (ELISA).The intestinal permeability was evaluated by fluorescein isothiocyanate-dextran (FITC-dextran) determination.The histomorphological changes in gut barrier were evaluated by Chiu grading system.Levels of acetylated lysine at the ninth position of histone 3 protein (Ac-H3K9),hypoxia-inducible factor 1α (HIF-1α),zona occludens 1 (ZO-1) and myosin light chain kinase (MLCK) were determined by immunofluorescence staining and Western Blot.Results Compared with sham + NS group,rats in scald + NS group showed intestinal mucosal damage 2 hours after bum injury,as well as increased mucosal permeability,protein expression levels of HIF-1 α,VEGF,MLCK,and lowered levels of AC-H3K9 and ZO-1.These changes were much more prominent at 6 hours after injury.VPA treatment significantly attenuated the bum-induced intestinal damage.Compared with scald + NS group,the protective effects in scald + VPA group was not evident at 2 hours after injury;however,intestinal damage was much less severe at 6 hours after injury (Chiu score:2.03 ± 0.27 vs.3.12 ± 0.15),intestinal permeability was significantly decreased [FITC-dextran (μg/L):709 ± 76 vs.1138 ± 75],histone acetylation was enhanced [Ac-H3K9 (gray value):1.55 ± 0.12 vs.0.48±0.12],ZO-1 degradation was significantly inhibited (gray value:0.69 ± 0.12 vs.0.43 ± 0.16),the protein expression levels of VEGF and MLCK were significantly down-regulated [VEGF (ng/mg):51.7±3.7 vs.71.2±4.3,MLCK (gray value):1.98±0.20 vs.2.80±0.24],while the HIF-1 α protein expression levels were significantly reduced at both 2 hours and 6 hours after injury (gray value:2.50±0.39 vs.3.88±0.42 at 2 hours,1.83±0.42 vs.4.42±0.41 at 6 hours,all P < 0.05).Conclusions Severe bum injury can induce histone deacetylation,ZO-1 degradation and intestinal barrier dysfunction.VPA can improve the levels of histone acetylation and ZO-1,and protect intestinal epithelial barrier function.These may probably be mediated through inhibiting HIF-1α and its downstream gene VEGF and MLCK.
3.Transcriptome profiling and analysis of Panax japonicus var. major.
Shao-peng ZHANG ; Jian JIN ; Bing-xiong HU ; Ya-yun WU ; Qi YAN ; Wan-yong ZENG ; Yong-lian ZHENG ; Zhang XI-FENG ; Ping CHEN
China Journal of Chinese Materia Medica 2015;40(11):2084-2089
The rhizome of Panax japonicus var. major have been used as the natural medicinal agent by Chinese traditional doctors for more than thousand years. Most of the therapeutic effects of P. japonicus var. major had been reported due to the presence of tetracyclic or pentacyclic triterpene saponins. In this study, Illumina pair-end RNA-sequencing and de novo splicing were done in order to understand the pathway of triterpenoid saponins in this species. The valid reads data of 15. 6 Gb were obtained. The 62 240 unigenes were finally obtained by de novo splicing. After annotation, we discovered 19 unigenes involved in ginsenoside backbone biosynthesis. Additionally, 69 unigenes and 18 unigenes were predicted to have potential function of cytochrome P450 and UDP-glycosyltransferase based on the annotation results, which may encode enzymes responsible for ginsenoside backbone modification. This study provides global expressed datas for P. japonicus var. major, which will contribute significantly to further genome-wide research and analysis for this species.
Gene Expression Profiling
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Panax
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genetics
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Saponins
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biosynthesis
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Sequence Analysis, RNA
4.A cost-effectiveness analysis of comprehensive rehabilitation treatment of hand burn.
Nan YI ; Bing-shui WANG ; Da-hai HU ; Xiong-xiang ZHU ; Meng-na SHI
Chinese Journal of Burns 2009;25(6):422-425
OBJECTIVETo observe the effect of comprehensive rehabilitation treatment on hand burn, and to make a cost-effectiveness analysis.
METHODSSixty-two patients with ninety-eight affected hands were divided into rehabilitation group (32 cases, 48 hands) and control group (30 cases, 50 hands). Patients in rehabilitation group received comprehensive rehabilitation treatment at early stage after burn; patients in control group were given instructions for function training at the same time. The functions of the hands to be restored including grasp, hold, pinch, nip, forearm pronation and supination, fetching, laying, and writing abilities of patients in both groups were quantitatively evaluated with Carroll's upper extremity function test before treatment and 5 months after. Direct medical costs of patients in both groups within 5 months were respectively added up to make a cost-effectiveness analysis.
RESULTSIn rehabilitation group, function of digital opposition, palmar opposition, holding, and pinching of 37 hands recovered well, with which patients could pick food, put on clothes, go to toilet, and self-care etc. independently. Function of digital opposition, palmar opposition, holding, pinching half recovered in 7 hands, accompanied with well recovered of metacarpophalangeal function, but recovery of function of interphalangeal joint was less satisfactory. Although patients could grasp and hold, they were still poor in fine and harmonized activities. Joint ranges of motion of 4 hands were poor with limited function, and this was resulted from not strictly following treatment for remaining granulation wound. In control group, 23 hands received reconstructive surgery, 14 of them recovered with good function, but were poor in most of fine and harmonized activities. Severe claw hands were found in 13 hands. The ratio between total mean cost value and total function increment value in rehabilitation group (181 +/- 11) was obviously lower than that in control group (298 +/- 30, P < 0.01).
CONCLUSIONSComprehensive rehabilitation treatment at early stage after hand burn has a good effect on prevention and treatment of hand deformity, promoting recovery of hand function and improving hand appearance. It is also less costly.
Adolescent ; Adult ; Burns ; rehabilitation ; Child ; Child, Preschool ; Cost-Benefit Analysis ; Female ; Hand Injuries ; rehabilitation ; Humans ; Male ; Middle Aged ; Rehabilitation ; economics ; Treatment Outcome ; Young Adult
5.2019 Novel Coronavirus (COVID-19) Pneumonia: Serial Computed Tomography Findings
Jiangping WEI ; Huaxiang XU ; Jingliang XIONG ; Qinglin SHEN ; Bing FAN ; Chenglong YE ; Wentao DONG ; Fangfang HU
Korean Journal of Radiology 2020;21(4):494-497
From December 2019, Coronavirus disease 2019 (COVID-19) pneumonia (formerly known as the 2019 novel Coronavirus [2019-nCoV]) broke out in Wuhan, China. In this study, we present serial CT findings in a 40-year-old female patient with COVID-19 pneumonia who presented with the symptoms of fever, chest tightness, and fatigue. She was diagnosed with COVID-19 infection confirmed by real-time reverse-transcriptase-polymerase chain reaction. CT showed rapidly progressing peripheral consolidations and ground-glass opacities in both lungs. After treatment, the lesions were shown to be almost absorbed leaving the fibrous lesions.
6.2019 Novel Coronavirus (COVID-19) Pneumonia: Serial Computed Tomography Findings
Jiangping WEI ; Huaxiang XU ; Jingliang XIONG ; Qinglin SHEN ; Bing FAN ; Chenglong YE ; Wentao DONG ; Fangfang HU
Korean Journal of Radiology 2020;21(4):501-504
From December 2019, Coronavirus disease 2019 (COVID-19) pneumonia (formerly known as the 2019 novel Coronavirus [2019-nCoV]) broke out in Wuhan, China. In this study, we present serial CT findings in a 40-year-old female patient with COVID-19 pneumonia who presented with the symptoms of fever, chest tightness, and fatigue. She was diagnosed with COVID-19 infection confirmed by real-time reverse-transcriptase-polymerase chain reaction. CT showed rapidly progressing peripheral consolidations and ground-glass opacities in both lungs. After treatment, the lesions were shown to be almost absorbed leaving the fibrous lesions.
Adult
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China
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Coronavirus
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Fatigue
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Female
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Fever
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Humans
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Lung
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Pneumonia
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Thorax
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Tomography, X-Ray Computed
7.The overview of the property system and characterization techniques of Chinese medicine materials before the pharmaceutical
Zhi-wei XIONG ; Ru-xi NING ; Ying-xia ZHAO ; Xiao-xin HU ; Bing YANG ; Yuan-pei LIAN ; Liang FENG ; Xiao-bin JIA
Acta Pharmaceutica Sinica 2021;56(8):2048-2058
At present, the modernization of Chinese medicine preparations (CMPs) is still a challenging task. The 3 typical Chinese medicine materials (CMMs) used for preparing CMPs are the powders, extracts, and components of Chinese medicine and their properties of CMMs are important for designing CMPs. Basing on our long term research, we have established a property system for CMMs according to the state of CMMs under an exactly condition and according to the interaction characteristics between substances. The property system could be divided into 5 categories: material composition, spatial structure, body property, surface property, physicochemical properties, and they could also be divided into 18 subcategories. Furthermore, we also established the corresponding index and characterization system, where the 61 indexes and characterization techniques were systematically summarized. At last, we hope that the article will promote the modernization of CMPs.
8.Rapid simultaneous detection of Salmonella and Shigella using modified molecular beacons and real-time PCR.
Xiao-lu SHI ; Qing-hua HU ; Jia-feng ZHANG ; Qing-ge LI ; Bing WANG ; Yi-man LIN ; Zhi-xiong ZHUANG ; Xiao-li LIU ; Shun-xiang ZHANG
Chinese Journal of Epidemiology 2006;27(12):1053-1056
OBJECTIVEDual detection of Salmonella and Shigella using modified molecular beacons and real-time PCR was developed. The established method was applied to rapid diagnosis of Salmonella and Shigella' food poisoning, and for routine monitoring programs.
METHODSTwo sets of primers were designed based on the core sequence of invA gene and ssaR gene published on GenBank to detect Salmonella, and ipaH gene were selected to detect Shigella. Three corresponding modified molecular beacons labeled with different fluorophors were designed. The molecular beacons and primer sets were tested against numerous strains from 55 different bacterial species. Then the two assays were combined to establish the dual real-time PCR assay, and were applied to the food poisoning diagnosis and surveillance.
RESULTSFor the modified molecular beacons-based dual real-time PCR assay, the sensitivity achieved was 69-93 fg/microl, 32-64 CFU/ml or 1-2 CFU/PCR reaction. There was no cross-reaction with other bacteria served as control. The dual real-time PCR assay was used to detect 134 Salmonella strains and 67 Shigella strains but no false signals were observed. 1100 food poisoning samples were tested with 569 Salmonella and 42 were Shigella identified by real time PCR. Among the positive samples, 551 were detected Salmonella and 41 were Shigella by traditional culture method. The overall test could be finished within 2 hours to one day starting from sample preparation.
CONCLUSIONThe modified molecular beacons-based dual real-time PCR assay was rapid, sensitive, and specific. It could be applied to the rapid diagnosis of Salmonella and Shigella' food poisoning.
DNA Primers ; Dysentery, Bacillary ; diagnosis ; Genes, Bacterial ; Humans ; Polymerase Chain Reaction ; methods ; Salmonella ; genetics ; Salmonella Food Poisoning ; diagnosis ; Sensitivity and Specificity ; Shigella ; genetics
9.A surface-modified biodegradable urethral scaffold seeded with urethral epithelial cells.
Wei-Jun FU ; Zhong-Xin WANG ; Gang LI ; Bing-Hong ZHANG ; Lei ZHANG ; Kun HU ; Bao-Fa HONG ; Xiao-Xiong WANG ; Fu-Zhai CUI ; Xu ZHANG
Chinese Medical Journal 2011;124(19):3087-3092
BACKGROUNDEfficient cell adhesion and proliferation is a central issue in cell-based tissue engineering, which offers great promise for repair of urethral defects or strictures. This study evaluated the adhesion and growth of rabbit uroepithelium on a surface-modified three-dimensional poly-L-lactic acid (PLLA) scaffold.
METHODSUrethral mucosa were harvested from male New Zealand rabbits and the urothelium were dissociated and then cultured. Immunocytochemistry on cultured uroepithelium for pancytokeratin and uroplakin II and TE-7 confirmed pure populations. After in vitro proliferation, cells were seeded onto a surface-modified urethral scaffold with non-knitted filaments. The morphology and viability of the cells were examined by immunohistochemical and fluorescence staining. Inverted and scanning microscopes were used to document cell growth and adhesion.
RESULTSThree to five days after primary culture, the uroepithelial cells gradually became confluent, assuming a cobblestone pattern. The filaments of the urethral scaffold had excellent biocompatibility and allowed growth of the uroepithelium, without affecting viability. The uroepithelial cells adhered to and grew well on the scaffold. After 3 - 7 days, the cells grew vigorously and meshes of the scaffold were full of uroepitheliums.
CONCLUSIONSThe surface-modified urethral scaffold with non-knitted filaments allows the growth of uroepithelium and can serve as a carrier for the tissue engineering of urethra.
Absorbable Implants ; Animals ; Cells, Cultured ; Epithelial Cells ; physiology ; Lactic Acid ; Male ; Polyesters ; Polymers ; Rabbits ; Tissue Engineering ; methods ; Urethra ; cytology
10.Correlation of the content and expression of urokinase plasminogen activator with asthenospermia in rat models.
Yan LIU ; Xue-Bing PANG ; Jing-Jing LIAO ; Lian HU ; Cheng-Liang XIONG
National Journal of Andrology 2008;14(9):786-791
OBJECTIVETo investigate the content and expression of the urokinase plasminogen activator (uPA) in the ornidazole-induced asthenospermia animal model, and to probe the mechanism of ornidazole inducing asthenospermia and the possibility of using uPA for the prevention and treatment of asthenospermia.
METHODSForty-eight male rats were equally randomized into 5 medication groups (1 d, 5 d, 10 d, 15 d and 20 d) and a blank control group, and ornidazole (200 mg/kg) was given intragastrically every day to the former five while 0.5% carboxymethylcellulose Na (CMC-Na) to the latter for 20 successive days. Then the rats were sacrificed by intraperitoneal injection of pentobarbital at 1, 5, 10, 15 and 20 days respectively and the epididymides and testes harvested. The integrity of the sperm cell membrane was detected by hypoosmotic swelling experiments, the uPA expression in the testicular and epididymal tissues dynamically observed by immunohistochemistry and the level of uPA mRNA in the testis determined by RT-PCR.
RESULTSThe integrity of the sperm cell membrane was reduced at 10 days and remained low till the end of the medication, but with no statistic significance. Compared with the blank controls, the uPA expression and mRNA content in the testicular and epididymal tissues showed no conspicuous difference in the 1 d and 5 d groups, decreased insignificantly in the 10 d group, but significantly in the 15 d and 20 d groups (P < 0.05).
CONCLUSIONThe defect of sperm cell membrane and decrease of sperm motility go in parallel with the reduced expression and content of uPA, which may be one of the factors for the development of asthenospermia.
Animals ; Asthenozoospermia ; metabolism ; Male ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley ; Sperm Motility ; Spermatozoa ; cytology ; Urokinase-Type Plasminogen Activator ; biosynthesis ; metabolism