ObjectiveTo compare the outcomes of standard large craniectomy with routin craniectomy in 65 patients with severe traumatic brain injury. Methods65 patients were randomly divided into standard large craniectomy group(41 cases) and routine craniectomy group (24 cases), and the effect was compared between the two groups. ResultsThe outcome showed 17 cases of good recovery,6 cases of moderate disability,5 cases of severe disability,2 cases of persistent vegetative status,l1 cases of death in the standard group and 5 eases of good recovery,3cases of moderate disability,4 cases of severe disability, 1 of persistent vegetative status, 12 cases of death in route group. ConclusionThe standard flesh wound big bone petal reduced pressure technique could enable the brain organization to obtain the full reduced pressure, effectively protect the brain function, after the distinct improvement heavy eranioeerebral damages, reduce the case fatality rate. But, this method also had certain shortcoming, must therefore grasp the surgery to refer to drafts.

AIM: To establish an HPLC for determining notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 in Xueshuantong Injection (total saponin of Radix et Rhizoma Notoginseng).METHODS: The analysis was performed on UltimateTM XB C18 column(4.6 mm ? 250 mm,5 ?m) with a gradient mobile phase of acetonitrile(A)water(B) (0-30 min,20% A→38% A;30-31 min 38% A→20% A;31-40 min,20% A) at a flow rate of 1.0 mL/min.The column temperature was maintained at 40 ℃,and the detection wavelength was set at 203 nm.RESULTS:The linear range of notoginsenoside R1 fell within 0.40 -4.01 ?g (r =0.999 9 ) and the average recovery was 99.36 % (RSD = 0.72% ,n = 6).The linear range of ginsenoside Rg1 fell within 1.47-14.69 ?g (r = 0.999 9) and the average recovery rate was 99.41% (RSD = 0.52% ,n = 6).The linear range of ginsenoside Rb1 fell within 1.50-15.03 ?g(r = 0.999 9) and the average recovery rate was 99.47% (RSD = 0.50% ,n = 6).CONCLUSION: This method is accurate,simple,rapid and can be used for the quality control of Xueshuantong Injection.

125 Wistar rats were divided into five groups randomly. The experimental period was 20 weeks. The results showed that both in the rats drinkcd water containing 6mM Li_2CO_3. and the rats with iodine-deficiency alone, their thyroid gland increased in weight and showed a pattern of hyperplatic goitre; their serum T, dropped which T_3 was increased. These changes were more marked in the rats with iodine deficiency and the activity of thyroid superoxidas strengthened. But in the rats that drinked water containing 6mM Li_2CO_3 there was more colloid in the follicular lumens of the thyroid. Under the condition of iodine deficiency, the rats that drinkcd water containing 3mM and 6mM Li_2CO_3 had larger and heavier thyroid gland, and their serum T_4 dropped which T_3 remained normal. Compared with the rats which had iodine deficiency along, their thyroid glands showed a typical picture of colloid goitrc. In the iodine deficiency rats which drinked water containing 3mM Li_2CO_3 the change of the thyroid gland was even more marked.

S-phase kinase associated protein (Skp2) assumes the high expression in the most of malignant tumors, with its occurrence, and the development and the prognosis is closely related with a latent diagnose and treat went value. This review will focus on unique feature, biology activity, the function mechanism and with malignant tumor relations of the Skp2.

Female ; Humans ; Pregnancy ; Pregnancy Complications ; diagnosis ; therapy ; Thyroid Nodule ; diagnosis ; therapy

Objective To explore the medication rules of State Medical MasterYan Zhenghua. Methods The prescriptions containing Radix Paeoniae Alba that prescribed by Pro. Yan were collected to build a database based on traditional Chinese Medicine(TCM)inheritance assist system. After analyzed by the statistical reports module and the data analysis module which were from TCM inheritance assist system, and the methods of data-mining that including association rules and apriori algorithm, the frequency of single medicine, the frequency of drug combination, the association rules between drugs and core drug combinations which all were including Radix Paeoniae Alba can be get from the database.Results The prescriptions including Radix Paeoniae Alba were commonly used to treat megrim, stomach-ache, acid regurgitation and other syndromes. The highest frequency used drugs were Radix PaeoniaeRubra, PericarpiumCitriReticulatae, Radix SalviaeMiltiorrhizae, Radix AngelicaeSinensis, Poria, and so on. The most frequency drug combinations were “RadxPaeoniae Alba, Radix PaeoniaeRubra”, “Radix SalviaeMiltiorrhizae, Radix Paeoniae Alba”, and “Radix Paeoniae Alba, PericarpiumCitriReticulatae”. The drug association rules that the confidence was more than once were “Radix PaeoniaeRubra - Radix Paeoniae Alba”, “OsDraconis - Concha Ostreae”, “Radix AngelicaeSinensis,RadixPaeoniaeRubra - Radix Paeoniae Alba”, and “Radix PaeoniaeRubra, HerbaTaxilli - Radix Paeoniae Alba”, and so on.Conclusions The drugs in the prescriptions containing Radix Paeoniae Alba that built by Pro. Yan mostly had the effects of enriching the blood and invigorating the circulation of blood, relieve uneasiness of mind and body tranquilization, which reflected the clearly thought when constructing prescriptions.

Child ; Cystitis ; Eosinophils ; Humans ; Male

Objective To investigate the effect of fluoride on expression of Runx2 mRNA and protein in bone tissue of rats. Methods Fourteen SD rats were randomly divided into two groups: control group(tap water with fluoride ＜ 0.06 mg/L), and fluorosis group(fluoride 50 mg/L in water). After 4 moths, expressions of both mRNA and protein of Runx2 in rat bone tissue were determined by RT-PCR and Western blotting. Results The results showed that the expression of Runx2 mRNA and protein in fluoride-treated bone tissue were 2.287 ± 0.261 and 0.929 ± 0.229, respectively, both of which were significantly higher than those of control group(0.995 ± 0.123,0.317 ± 0.068, t = 11.85,6.78, P ＜ 0.05). Conclusions Fluoride can increase the expression of Runx2 mRNA and protein in bone tissue of rats, and Runx2 may be involved in the pathogenesis of bone injury caused by fluoride.

Uveitis is a group of inflammatory diseases causing serious impairment of visual function. The precise etiology and pathogenesis remain unclear. lt can be induced by infection, trauma, tumor or autoimmune response, of which autoimmune response is the most common. ln recent years, the cytokines in the process of inflammatory autoimmune diseases role has become one of research hot spots, its role in the pathogenesis and the relationship between the disease activity more and more attention. This article reviews the effects of interleukin, tumor necrosis factor, interferon and transforming growth factor-β in the pathogenesis of uveitis.

Objective To explore the mechanism of long chain noncoding RNA RORin regulating prolifer-ation and apoptosis of pancreatic cancer cell. Methods Pancreatic cancer cell line BxPC-3 was selected. The RNA level of lncRNA ROR and notch1 was detected by RT-PCR.Notch1 protein level was detected by Western blot. The regulating relationship between lncRNA ROR and notch1 was analyzedby RNAhybird and luciferase re-porter assay. At last ,CCK-8 and TUNEL were applied to detectthe proliferation and apoptosis of cell line. Re-sults lncRNA ROR and notch1 were highly expressed in pancreatic cancer tissue ,compared with normal tis-sues. There was positive correlation between them. lncRNA ROR was over-expressed in BxPC-3,cell proliferation activity was increased and the percentagesof DNA damaged positive cells was decreased ,accompanied by in-creased levels of notch1 mRNA and protein. Luciferase assay confirmed that ROR could bind to notch1and inhibit its activity by miR-137. Compared with control group ,the proliferation of pcDNA-ROR + si-notch1 cells reduced and the proportion of TUNEL positive cells increased. The differences were statistically significant. Conclusionl ncRNA ROR regulated the proliferation and apoptosis of pancreatic cancer cells by promoting the expression of notch1.