1.The 16S rDNA Sequence Analysis and Phenotypical Study of Strain F12-11-1-2
Xiao-Yu LIU ; Qiang-Zhi XU ; Yu YANG ; Feng AI ; Bing-Hua JIAO ;
Microbiology 1992;0(01):-
The strain F12-11-1-2 was isolated from the East China Sea,which had antimitosis activity using Pyricularia oryzae mode.Ac- cording to phenotypical study,salt-aggregation test and 16S rDNA sequence analysis,the strain F12-11-1-2 has been identified to be Bacillus subtilis.
2.The effect of fluoride and arsenic pollution on bone metabolism in exposed population
Qi-bing, ZENG ; Yun, LIU ; Ai-hua, ZHANG ; Feng, HONG ; Jun NG YA ; Xian, YU
Chinese Journal of Endemiology 2011;30(4):393-395
Objective To explore the effect of fluoride and arsenic pollution on bone metabolism in exposed population. Methods One hundred and fifty-two fluoride and arsenic exposed people were selected from Jiaole village, Yuzhang town, Xingron county, Guizhou province in 2006, and 59 not exposed people from Daguoduo village 13 km away from Jiaole village were selected as control. Urinary fluorine(UF), urinary arsenic (UAs), urinary hydroxyproline (UHYP), cross-linked N-telopeptides of type I collagen (UNTX) and bone strength index(STI) were detected. Results The main effect of fluoride on UHYP and UNTX were statistically significant (F = 9.785, 4.225, P < 0.01 ), but was not significant on STI(F = 0.183, P > 0.05). The main effect of arsenic on UNTX was statistically significant (F = 2.660, P < 0.05 ), but was not significant on UHYP and STI(F = 2.012, 0.183,all P > 0.05). The interaction between fluoride and arsenic on UNTX was statistically significant (F= 2.429, P <0.01), but was not significant on UHYP and STI(F= 1.218, 1.001, all P> 0.05). Conclusions Fluoride exposure can affect the metabolism of collagen and bone resorption, and Arsenic exposure main affect bone resorption, fluoride and arsenic co-exposure have more significant effect on bone resorption. UNTX may be used as biological biomarker of bone metabolism for population co-exposed to fluoride and arsenic in health monitoring.
3.Effect of language rehabilitation training no stroke patients with complete aphasia
Bing YAN ; Chunnuan HUO ; Zengzhi YU ; Yan' ; ai MA ; Chunyan ZHU ; Xiaopeng GUO ; Saichun ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2004;10(12):745-746
ObjectiveTo observe the effect of language rehabilitation training on stroke patients with complete aphasia.Methods10 stroke patients with complete aphasia were treated with language rehabilitation training.ResultsAfter training, scores of hearing, naming, reciting, reading, and writing of patients were significant higher than that before training (P<0.05—0.01).Conclusion Language rehabilitation training plays an active role in rehabilitation of stroke patients with complete aphasia.
4.Transposition of Branches of Radial Nerve Innervating Supinator to Posterior Interosseous Nerve for Functional Reconstruction of Finger and Thumb Extension in 4 Patients with Middle and Lower Trunk Root Avulsion Injuries of Brachial Plexus
WU XIA ; CONG XIAO-BING ; HUANG QI-SHUN ; AI FANG-XIN ; LIU YU-TIAN ; LU XIAO-CHENG ; LI JIN ; WENG YU-XIONG ; CHEN ZHEN-BING
Journal of Huazhong University of Science and Technology (Medical Sciences) 2017;37(6):933-937
This study aimed to investigate the reconstruction of the thumb and finger extension function in patients with middle and lower trunk root avulsion injuries of the brachial plexus.From April 2010 to January 2015,we enrolled in this study 4 patients diagnosed with middle and lower trunk root avulsion injuries of the brachial plexus via imaging tests,electrophysiological examinations,and clinical confirmation.Muscular branches of the radial nerve,which innervate the supinator in the forearm,were transposed to the posterior interosseous nerve to reconstruct the thumb and finger extension function.Electrophysiological findings and muscle strength of the extensor pollicis longus and extensor digitorum communis,as well as the distance between the thumb tip and index finger tip,were monitored.All patients were followed up for 24 to 30 months,with an average of 27.5 months.Motor unit potentials (MUP) of the extensor digitorum communis appeared at an average of 3.8 months,while MUP of the extensor pollicis longus appeared at an average of 7 months.Compound muscle action potential (CMAP) appeared at an average of 9 months in the extensor digitorum communis,and 12 months in the extensor pollicis longus.Furthermore,the muscle strength of the extensor pollicis longus and extensor digitorum communis both reached grade Ⅲ at 21 months.Lastly,the average distance between the thumb tip and index finger tip was 8.8 cm at 21 months.In conclusion,for patients with middle and lower trunk injuries of the brachial plexus,transposition of the muscular branches of the radial nerve innervating the supinator to the posterior interosseous nerve for the reconstruction of thumb and finger extension function is practicable and feasible.
5.Effects of different transplanting conditions on survival rate and growing status of Anoictochilus roxburghii plantlets.
Qing-Song SHAO ; Ai-Cun ZHOU ; Yu-Qiu HUANG ; Ying-Lei DONG ; Bing-Kang HU ; Ming-Yan LI
China Journal of Chinese Materia Medica 2014;39(6):955-958
The growing status of Anoectochilus roxburghii seedling was observed and the survival rate of seedlings, height, stem diameter and plant fresh weight under the conditions of different transplanting substrate compositions, planting density, shading rate were measured. The results showed that the effects of different transplanting substrates, planting densities, shading rates and nutrient solutions on the growing status of A. roxburghii plantlets varied greatly. A. roxburghii plantlets demonstrated a high survival rate and better growing status under the Following conditions: the ratio of peat and river sand as 2: 1, the planting density as 3 cm x 3 cm, the shading rate as 70%, and the nutrient solution as 1/4MS. The findings of the study provide a solid technical solution for the artificial cultivation of A. roxburghii plantlets.
Breeding
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methods
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Culture Media
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chemistry
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pharmacology
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Orchidaceae
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drug effects
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growth & development
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Seedlings
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drug effects
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growth & development
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Survival Analysis
6.Expression of Tumstatin_(183-230)-TRAIL fusion protein and identification of its biological functions
Na REN ; Liang-Hua WANG ; Yun GAO ; Ming-Juan SUN ; Yu-Liang JIAO ; Ai-Yun GUO ; Bing-Hua JIAO ;
Academic Journal of Second Military Medical University 1985;0(05):-
Objective:To express Tumstatin_(183-230)-TRAIL fusion protein and to observe its biological functions.Methods: SOE-ing PCR was employed to amplify the recombinant sequence of Tumstatin_(183-230)and TNF-related apoptosis-inducing ligand (TRAIL_(114-281)).An expression vector pMAL-Tu-T was constructed by inserting Tu-T sequence into pMAL-c_2;the vector was used to transfect E.coli BL21(DE3)and expression of MBP-Tu-T fusion protein was induced by IPTG.Amylose Resin columns were employed to purify the fusion protein.The biological functions of MBP-Tu-T protein was examined by inhibitory test of endothelial cell proliferation,standard tumor cell cytotoxic assay,in vitro tube formation inhibition,and electron microscopic observation(apoptosis).Results:The expression rate of MBP-Tu-T fusion protein in E.coli was about 20%. Purified recombinant protein obviously inhibited endothelial cell proliferation(IC_(50)12.5?g/ml),induced apoptosis of pancreatic cancer cells,and inhibited tube formation.Conclusion:Constructed MBP-Tu-T fusion protein is bifunctional,which lays a solid foundation for further investigation of antitumor effect of Tumstatin_(183-230)-TRAIL in vivo.
7.Analysis on family function of community residents and its influential factors
Jin-Bing BAI ; Li-Ping YU ; Yan-Qun LIU ; Xia LIU ; Ai-Ling WANG
Chinese Journal of Modern Nursing 2009;15(1):33-35
Objective To explore the current status of the family function and the influential factors in the community, Methods A questionnaire survey including the general data questionnaire and APGAR scale developed by Smikstein was conducted in 94 families of three communities of Wuhan City. Results 46.8% of the surveyed family showed good function, 51. 1% showed moderate dysfunction and 2.1% in severe dysfunction. The multinomial regression revealed that education level and whether the family members had chronic diseases had influence to the family function level (P<0.05). Conclusions More than 50% of the families exist moderate or severe family dysfunction. The higher education level, the better family function; meanwhile compared with the family whose members experience chronic diseases, the family without experiencing chronic dieseases shows much better family function.
8.Effect of Contralateral Acupuncture on Motor Function in Acute Stroke Patients
Yong-Gang ZHU ; Bing-Yu AI ; Xiu-Hua ZHU ; Xin-Yu BAI ; Fei DONG
Shanghai Journal of Acupuncture and Moxibustion 2018;37(1):16-19
Objective To investigate the clinical efficacy of contralateral acupuncture in treating motor dysfunction in patients with acute ischemic stroke.Methods Sixty patients with acute ischemic stroke were randomized to treatment and control groups, 30 cases each. Both group received electroacupuncture in addition to conventional rehabilitation training. Besides, the treatment group received electroacupuncture at points on the healthy side in the morning and on the affected side in the afternoon and the control group, electroacupuncture at points on the affected side in both the morning and afternoon. Motor function was assessed using the Fugl-Meyer Assessment (FMA), the modified Barthel Index (MBI), integrated electromyography (iEMG) and median frequency (MF) in the two groups of patients before and after treatment.Results There were statistically significant pre-/post-treatment differences in the FMA score, the MBI score, iEMG and MF in the two groups (P<0.01). There were statistically significant post-treatment differences in the FMA score, the MBI score and iEMG between the treatment and control groups (P<0.01,P<0.05).ConclusionContralateral and healthy side acupuncture can markedly improve motor function in acute stroke patients.
10.Construction and expression of a fusion protein made of tissue-type plasminogen activator and hirudin in Pichia pastoris.
Ai-Ping YU ; Bing-Xing SHI ; Chun-Na DONG ; Zhong-Hua JIANG ; Zu-Ze WU
Chinese Journal of Biotechnology 2005;21(4):553-557
To combine the fibrinolytic with anticoagulant activities for therapy of thrombotic deseases, a fusion protein made of tissue-type plasminogen activator (t-PA) and hirudin was constructed and expressed in chia pastoris. To improve thrombolytic properties of t-PA and reduce bleeding side effect of hirudin, FXa-recognition sequence was introduced between t-PA and hirudin molecules.The anticoagulant activity of hirudin can be target-released through cleavage of FXa at thrombus site. t-PA gene and hirudin gene with FXa-recognition sequence at its 5'-terminal were obtained by RT-PCR and PCR respectively. The fusion protein gene was cloned into plasmid pIC9K and electroporated into the genome of Pichia pastoris GS115. The expression of fusion protein was induced by methanol in shaking flask and secreted into the culture medium. Two forms of the fusion protein, single-chain and double-chain linked by a disulfide bond (due to the cleveage of t-PA at Arg275-Ile276), were obtained. The intact fusion protein retained the fibrinolytic activity but lacked any anticoagulant activity. After cleavage by FXa, the fusion protein liberated intact free hirudin to exert its anticoagulant activity. So, the fusion protein is a bifunctional molecule having good prospect to develop into a new targeted therapeutic agent with reduced bleeding side effect for thrombotic diseases.
Animals
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Cloning, Molecular
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Electroporation
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Hirudins
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biosynthesis
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genetics
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Humans
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Pichia
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genetics
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metabolism
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Recombinant Fusion Proteins
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biosynthesis
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genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Tissue Plasminogen Activator
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biosynthesis
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genetics