1.Assessment of retinal and choroidal neovascularization by in vivo immunostaining
ying, CHEN ; bing, XIE ; qiang, WU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(05):-
Objective To explore the optimal method of qualitative and quantitative assessment of retinal neovascularization(RNV),choroidal neovascularization(CNV) and leakage from CNV. Methods Thirteen mice were randomly divided into RNV assessment group(n=7)and CNV assessment group(n=6).For RNV assessment, 3 mice were served as normal controls,and the other 4 were made into models of retinopathy of prematurity(ROP).At postnatal d16,mice in normal control group and 3 ROP model mice received intraocular injection with FITC labeled or unlabeled antibody and were sacrificed 12 h later.The retinas were flat mounted(FITC labeled antibody) or incubated with secondary antibody(unlabeled antibody) and then flat mounted.The other ROP model mouse was served as blank control.For assessment of CNV and leakage from CNV,6 mice(12 eyes) were induced into models of CNV,and were randomly divided into two groups.Seven and 14d after model establishment,mice were intraocularly injected with unlabeled antibody,and fluorescein sodium were intraperitoneally injected 12 h later.The area of CNV and that of CNV leakage were calculated. ResultsRetinas from eyes injected with FITC labeled antibody exhibited good resolution of ultrastructure of RNV,while retinas from eyes injected with unlabeled antibody showed selective staining of RNV with no background,greatly facilitating identification and quantification of RNV.Merging images of stained CNV with images of fluorescein sodium leakage from CNV revealed double labeled CNV surrounded by fluorescein that had leaked into surrounding tissues.The mean area of CNV and its leakage of d14 were significantly different from those of d 7(P
2.To analyze the risk factors of tetany in patients with hyperventilation syndrome
Daofeng YOU ; Qiuge QIAO ; Bing XIE ; Xiangming JIANG ; Ying CHEN
Tianjin Medical Journal 2016;44(6):763-765
Objective To explore the risk factors causing tetany in patients with hyperventilation syndrome. Meth?ods A total of 103 patients with hyperventilation syndrome treated in our hospital were included in this study. According to whether there was tetany, patients were divided into tetany group and non-tetany group. Values of gender, age, electrolyte, pH and p(CO2) were analysed between two groups. The factors of P<0.1 were engaged in binary Logistic regression. Logistic regression (Forward Wald) was used to analyze the risk factors of tetany in patients with hyperventilation syndrome. Re?sults In 103 patients there were 70 patients with tetany (68%), 33 patients without tetany(32%). The serum K+, serum phos?phorus and p(CO2) values were significantly lower in tetany group than those of non-tetany group (P<0.01), while the pH value was significantly higher in tetany group than those of non-tetany group (P<0.01). There were no significant differences in gen?der, age, serum Na+, serum Cl-, serum calcium (bound calcium and ionized calcium), ionized calcium and serum Mg2+levels be?tween two groups (P>0.05). It was revealed that the younger age, the lower level of the serum K+, serum phosphorus and p(CO2) were the risk factors of tetany through binary Logistic regression analysis. Conclusion The risk factors of tetany in patients with hyperventilation syndrome include younger age, lower level of serum K+and serum phosphorus and reduced p(CO2).
3.Design and Analysis on Improving Reliability of Hospital Information System
Shengxin WENG ; Shaoping CHENG ; Ying HUANG ; Bangtie XIE ; Bing WEI
Chinese Medical Equipment Journal 2004;0(09):-
Objective To discuss how to improve the stability,reliability and security of the core servers of hospital information system.Methods Flaws on security were analyzed objectively in hospital server system adopting 'PC server & local hardisk' scheme.New schemes were put forward including both hot standby scheme based on concentrated storage and full redundancy scheme based on'minicomputer & diskette array'.Results It is proved that the full redundancy scheme is reasonable in theory,and usable in practice.Conclusion The mode of'minicomputer & diskette array' can effectively enhance the stability,reliability and safety of core servers.
4.Diagnostic value of prospective electrocardiograph-gating of CT to congenital heart diseases: a Meta-analysis
Bing WEN ; Ying DU ; Qiuyi CAI ; Junshi XIE ; Liangbo HU ; Yingkun GUO
Chinese Journal of Applied Clinical Pediatrics 2017;32(1):54-58
Objective To evaluate the clinical diagnostic value of prospective electrocardiograph (ECG)-gating of CT cardiac angiography in congenital heart diseases of Chinese population through a Meta-analysis.Methods The articles were searched to study CT prospective ECG-gating in diagnosis of congenital heart disease from January 1995 to February 2016 in domestic and foreign publications.The study quality was assessed by the Quality Assessment for Diagnostic Accuracy Studies and the data extraction was performed.The software of Meta-disc1.4 was used for heterogeneity test.Different effect models were choosen according to the results of heterogeneity analysis.Meanwhile,this soft was used to calculate the sensitivity,specificity,likelihood ratio and its 95% confidence interval (CI),respectively.The forest maps and summary receiver operator characteristic (SROC) curve were drawn.In addition,the area under curve (AUC) was calculated.Results Twelve articles were included in the Meta-analysis.The study included 1 431 congenital heart malformations confirmed by surgery or cardiac catheterization angiography.CT prospective ECG-gating technique had no heterogeneity in sensitivity and specificity of congenital heart disease.The total sensitivity,the total specificity,positive likelihood ratios,negative likelihood ratios and its 95% CI of CT cardiac angiography were 96% (95% CI 94% to 97%),100% (95% CI 100% to 100%),365.94(95% CI 231.18 to 579.26),0.04(95% CI 0.03 to 0.05) with fixed effect model,respectively.The AUC of the SROC was 99.86%,Q =0.987 9.Conclusions Prospectively ECG-gating of CT cardiac angiography has high sensitivity and specificity in the diagnosis of congenital heart diseases.Its AUC of the SROC is large.It has high diagnostic value in congenital heart diseases.
5.Exploring medical continuing education for general practitioners in regional multihospital system
Yiting XU ; Jing LIN ; Jie SHAO ; Ying JIANG ; Ping WU ; Bing XIE
Chinese Journal of Medical Education Research 2012;(12):1269-1272
This article discussed on the continuing medical education for general practitioners within a nonprofit multihospital system.Literature review,statistical analysis and survey results were employed.Training situation for general practitioner at home and aboard was reviewed.Statistics on population,disease spectrum,present situation of community doctors in Luwan branch of Ruijin hospital was also analyzed.Results showed that current education system for general practitioners cannot catch up the increasing demand yet.Meanwhile patients expected well qualified and skillful physicians in the community healthcare centers.Reinforcement of continuing medical education for general practitioners was the stepping stone of a nonprofit multihospital system.Improving skills and qualifications of physician played an essential role in the urban network of medical services.
6.Isolation and 16S rRNA Gene Sequence Analysis of a Thermotolerant Halophilic Bacterium
Ying XIE ; Lian-Bing LIN ; Xiu-Ling JI ; Yun-Lin WEI ;
Microbiology 1992;0(02):-
A strain of thermotolerant halophilic bacteria YJ0238 was isolated from the salt well at 47℃ in the Kangning countryside where was located at the side of Lancang River in the Tibet Autonomous Region. Some physiological and biochemical properties were characterized.16S rRNA gene sequence of YJ0238 was amplified by PCR,and its nucleotide sequence was determined. Based on it’s physiological and biochemical properties,homology and phylogenetic analysis of 16S rRNA gene sequence,strain YJ0238 was identified as a subspecies of the species Idiomarina zobellii. The GenBank accession number of the 16S rRNA gene sequence of strain YJ0238 is EF693953. Until now,there were few reports on the study of high-temperature and high-salt microbial in domestics. The results of this study will provide research material and information for further studies in this area.
7.Expression of TGF-?_1 and Smad2/3 in kidney of STZ-induced diabetic nephropathy rats
Qin YANG ; Rujia XIE ; Bing HAN ; Ying XIAO ; Ting YANG ; Li FANG ; Yiguo LONG ; Guozhong ZHANG
Chinese Journal of Pathophysiology 2000;0(10):-
AIM: To study the role of TGF-?/Smad pathway in the development of renal fibrosis in diabetic nephropathy.METHODS: Rats were induced to diabetic nephropathy by using tail intravenous injection of STZ.The expression of TGF-?_1,Smad2/3 protein and mRNA in kidney were examined at 2,4,8 and 16 weeks after STZ induction.CTGF,collagen-Ⅲ,PAI-1 mRNA expression in kidney at 16 weeks of STZ-induced diabetic nephropathy and normal rats were studied by RT-PCR.RESULTS: Weak TGF-?_1,Smad2/3 protein were detected in normal renal tissues while strong TGF-?_1,Smad2/3 staining were observed in renal tissues of diabetic nephropathy(0.057?0.030/0.223?0.040;0.017?0.010/0.153?0.010,respectively,P
8.Clinical, pathological and dynamic imaging findings of patients with mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes
Shou-Qiang JIA ; Ying-Ying ZHANG ; Bing-Kun XIE ; Ming WANG ; Ying LI
Chinese Journal of Neuromedicine 2012;11(8):842-846
Objective To investigate the clinical, pathological and dynamic imaging characteristics of patients with mitochondrial myopathy,encephalopathy,lactic acidosis and stroke-like episodes (MELAS). Methods A retrospective analysis was performed on the clinical,pathological and dynamic imaging data of 10 patients with MELAS confirmed by muscle biopsy. Results The clinical manifestations included headache,seizures,nausea,vomiting,nystagrnus and visual disturbances.CT showed less lesions,and MRI could clearly show multiple lesions which mainly located in the temporal,parietal,occipital cortex and sub-cortex,having multifocal,asymmetric,migratory characterstics and not following the distribution of blood vessels.MRA showed no significant stenosis,and the lesion showed hyperperfusion and vasogenic edema,and Lae peak was visible.Muscle biopsy showed ragged red fiber optical microscope (RRF) and strongly SDH-reactive vessel (SSV),and electron microscope showed increased mitochondria number, and abnormal size and shape. Conclusion MELAS has certain clinical and imaging characteristics; by combining the muscle biopsy,we can diagnose the disease early and make differential diagnosis.
9.An Analysis of Self-reported Income Status and Related Determinants among Primary Care Providers in a County in Dali
Bing ZHANG ; Jia ZHOU ; Yong MAO ; Long CHEN ; Xi-Zhuo XIE ; Cheng-Huan SUN ; Xiao-Ling WEN ; Xin-Ying XIE
Journal of Kunming Medical University 2018;39(2):39-45
Objective To assess the income status of primary care providers and to explore the determinants of income in a county of Dali. Methods In August 2016, the questionnaire was employed to collect the data of income status of 191 rural health workers and 217 village doctors in the county. Results Through the study, we found that the income of rural health workers in the county was 34, 000 (26, 000, 46,000) yuan with a satisfaction rate of 62.3% (95% CI 55.4%~69.2%) and no change (74.7%) was seen in the income among majorities after implementing the Zero Mark-up Policy for essential medicines. For the village doctors, the income was 20,000 (15,000, 24,000) yuan with a satisfaction rate of 40.6% (95% CI 34.0%~47.1%) and a fall of the income was found in more than half of the doctors after the implement of the policy. Conclusion Health care workers in towns are quite satisfied with their income whereas those in health stations of villages are not content, compared with the average income at the national level. We should increase government's investments on grass-root healthcare team, improve the incentive pay plans and promote the integrated management of health facilities in towns and villages.
10.Granulomatous slack skin with anaplastic large cell lymphoma: report of a case.
Jian-Jun XIE ; Zhi-Qiang ZHOU ; Yan WANG ; Ying LI ; Ren-Ya ZHANG ; Yu-Bo REN ; Bing CHEN ; Geng-Yin ZHOU
Chinese Journal of Pathology 2011;40(4):267-268
Adult
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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CD2 Antigens
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metabolism
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CD3 Complex
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metabolism
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CD4 Antigens
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metabolism
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Cyclophosphamide
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therapeutic use
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Doxorubicin
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therapeutic use
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Follow-Up Studies
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Humans
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Lymphoma, Large-Cell, Anaplastic
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drug therapy
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metabolism
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pathology
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Lymphoma, T-Cell, Cutaneous
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drug therapy
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metabolism
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pathology
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Male
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Neoplasms, Second Primary
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drug therapy
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metabolism
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pathology
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Poly(A)-Binding Proteins
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metabolism
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Prednisone
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therapeutic use
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Skin Neoplasms
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drug therapy
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metabolism
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pathology
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T-Cell Intracellular Antigen-1
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Vincristine
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therapeutic use