0.05);the epididymal indexes were significantly increased 6,24 and 72 hours after formalin injection(P

Objective\ To study whether gonadotropin releasing hormone receptor (GnRHR)mRNA exist in rat submaxillary gland and it's gene sequence. Methods\ The total RNA isolated from rat submaxillary gland was amplified by RT\|PCR, the PCR products were identified by sequencing with Sanger's method. Results\ The specific amplified band of GnRHR mRNA was detected through agarose gel electrophoresis and gene sequence is identical to the sequence of GnRHR which has been reported in rat pituitary. Conclusion\ GnRHR can be produced by submaxillary and response for modulating biological function of submaxillary.\;

Objective To observe the effect of cervical plexus block combined with cervical vertebra traction treatment of cervical spondylosis of nerve root type .Methods 60 cases of nerve root type cervical spondylosis were divided into two groups by coin tossing:group A(n=32) cervical plexus block combined with cervical traction thera-py, group B( n=28) treated by cervical traction therapy ,according to the severity of pain compared two groups of treatment effect.Results after treatment,20d group 10d,30d,90d pain scores were (4.61 ±0.70)%,(3.71 ± 0.57)%,(3.30 ±0.65)%,(4.44 ±1.04)%,group B respectively (5.88 ±1.47)%,(5.61 ±1.35)%,(4.83 ± 0.86)%,(5.50 ±0.87)%,the difference between two groups was statistically significant (t=5.85,1.06,1.30, 7.51,all P<0.01).Conclusion The cervical plexus block combined with cervical traction for treatment of nerve root type of cervical spondylosis is better than the routine treatment of cervical traction ,which is suitable for promotion of primary health care units .

Objective To estimate the iron level in the patients with thrombocytosis for differential diagnosis.Methods A retrospective analysis on 98 thrombocytosis patients was performed by studying hemogram,iron staining in bone marrow and serum iron and ferritin level.Results In the patients whose platelet count were between 400?10(9)/L and 800?10(9)/L 40.8% were diagnosed as iron-deficient anemia,while in the patients whose platelet count were higher than 800?10(9)/L 81.5% were diagnosed as essential thrombocytosis(ET).Of 27 ET cases 2 had lower serum ferritin values but their serum iron levels were in normal range.Conclusion The patients with platelet count between 400?10(9)L and 800?10(9)/L may be secondary thrombocytosis,while the patients with platelet count higher than 800?10(9)/L may be diagnosed as ET,but other conditions such as iron deficiency should be excluded.

Objective To study the localizations and the quantity of GnRH receptor in stomach of Sprague-Dawley (SD) rats under stress.Methods The model of stress SD rats was established by fear. Then, stomachs were taken from the rats in acute stress group (2h-12h), chronic stress group (1d-4w) and the control group respectively.The localizations and the quantity of GnRH receptor in stomachs were detected using immunohistochemistry and Western blotting.Results The results of immunohistochemistry showed that immunoreactivity of GnRH receptor was displayed in the gastric parietal cells and the epithelial cells of the gastric pits in stomachs of rats in all groups. The immunoreactive materials were distributed in membrane and cytoplasm of all positive cells, but not in nuclei. Meanwhile, the results of Western blotting showed that the number of GnRH receptor decreased significantly when SD rats were in stress from 2h to 2w (P

Objective To evaluate the curative effects of portal vein tumor emboli(PVTE)of hepatocellular carcinoma treated by CT-guided percutaneous ethanol injection(PEI).Methods Absolute ethanol was injected into the tumor embolus of portal vein guided by CT in twenty patients with hepatocellular carcinomas.The procedure was carried out one or two times each week one to three times as a course and one to two courses for a patient.The interval between two courses was one month and the patients were followed up for 6 months-5 years.Results Among the twenty patients,17(85%)were improved in different degrees after the treatment,with disappearence of the tumor emboli in 2(10%)and size stability or even smaller in 15 (75%),and finally no response in 3(15%).Conclusions CT-guided PEI is an effective method for patient with PVTE and proper selection of patient for the procedure is the key to obtain better curative effects.

OBJECTIVETo investigate the malignancy incidence among rubber manufacture and preliminary processing industrial workers in Hainan Province of China.

METHODSA retrospective survey was conducted on 9 farms in Hainan covering workers who got malignant tumor between January 1, 2005 and October 31, 2010.

RESULTOn average there were 10 320 workers worked on the 9 farms each year. Among them, 167 cases of malignancy were found. The morbidity was 279.01 every a hundred thousand and standardized morbidity was 159.03 every a hundred thousand; In comparison, we also investigated 14228 non-rubber-industry-related workers. 94 malignancy cases were found. The morbidity was 113.91 every a hundred thousand and standardized morbidity was 64.93 every a hundred thousand. The difference was statistically significant (P<0.01) meaning that rubber industrial workers in Hainan Province had a higher possibility of getting malignancy. Top 5 types of cancer found in this study were lung cancer (17.96%), liver cancer (17.36%), stomach cancer (13.17%), rectal cancer and nasopharyngeal carcinoma. 116 cases of these five types of cancer took up 69.45% of the total.

CONCLUSIONSRubber industrial workers in Hainan have a higher morbidity of malignancy than other local industries. But it is similar to other areas in China.

Adult ; Aged ; Aged, 80 and over ; China ; epidemiology ; Female ; Humans ; Incidence ; Male ; Middle Aged ; Neoplasms ; epidemiology ; Occupational Exposure ; Retrospective Studies ; Rubber ; Young Adult

Objective To study the expression of gonadotropin releasing hormone receptor (GnRH receptor) in the stomach of Sprague-Dawley rat exposed to light stress. Methods We established illumination stressed models that the rats were exposed to continuous light(totally 64 rats, 32 rats for experiment and control group, respectively). Then,stomachs were taken from the rats when the rats exposed to continuous light for 1 day, 2 days, 3 days, 4 days, 1 week, 2 weeks, 3 weeks, 4 weeks and their control groups, respectively.The localization and the expression of GnRHR and GnRHR mRNA in stomachs mucous membrane were detected using immunohistochemistry, Western blotting and real-time PCR. Results Immunoreactivity was displayed mostly in the parietal cells in gastric gland. There were not differences of this distribution in all groups of constant illumination and their control. The immunoreactive materials were distributed on membrane and in cytoplasm of all positive cells, but not in nuclei. The level of GnRHR in rats exposed to continuous illumination were higher from one week to four weeks compared with that in control(P<0.05), and the level of GnRHR reached the peak when rats were exposed to constant illumination for two weeks (P<0.01). The quantity of GnRH mRNA in rats exposed to continuous illumination were higher from three weeks to four weeks compared with that in control(P<0.05). Conclusion The expression of GnRHR in digestive tract was effected by illumination stress.GnRH might regulate digestive function by interaction with GnRHR, suggesting that GnRH may be acted as a hormone, not only responded to normal physiological function of digestive tract but also responded to stress activity.

Objective To investigate the changes of the weight of testis and epididymis,sperm numbers in cauda of epididymis,the structure of testis and the expression of 3?-HSD in rat testis after removing submandibular gland in rats.Methods On the day 14,28 and 42 after the operation,the testis and the epididymis were weighed and the epididymis sperm were counted.The changes of the testis were showed by HE stain.The changes of 3?-HSD expression were analyzed by immunohistochemistry.Results On the day 28 and 42 after the operation,the weight of body,testis and epididymis decreased markedly(P0.05),but increased apparently on the day 28(P