1.Effects of DingKunDan on the endometriosis model rats
Runhua HE ; Wenyan WANG ; Bing WEI
Acta Universitatis Medicinalis Anhui 2015;(11):1693-1695
We built rats model of endometriosis applying autologus endometrial transplants to compare the graft vol-ume of the modeling rats before and after dosing,serum tumor necrosis factor alpha(TNF-α)levels in rats,and the expressions of vascular endothelial cell growth factor(VEGF)in ectopic endometrium.To compare with the control group,the graft volume of DingKunDan group was reduced and the serum TNF-αlevel declined ,and also the ex-pression of VEGF in ectopic endometrium was reduced.Their differences were statistically significant (P <0.05). DingKunDan can inhibit the growth of ectopic endometrium in endometrosis model rats.The mechanism may be re-lated to the decrease of the serum TNF-αlevel and the VEGF expression.
2.Diagnostic value of MSCT in nasal septum fracture.
Yong-bin HE ; Wei-guo JIANG ; Bing XU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(11):870-871
Adolescent
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Adult
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Child
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Female
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Humans
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Male
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Nasal Septum
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injuries
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Skull Fractures
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diagnostic imaging
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Tomography, Spiral Computed
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Young Adult
3.Investigationof translatingDICOMimage into BMP image
Chengbo WANG ; Wei CHEN ; Bing XIE ; Enquan ZHANG ; Qiang HE
Chinese Medical Equipment Journal 1989;0(01):-
The structures of DICOM image and BMP image are introduced through the analyses of them and DICOM standard.The method of translating DICOM image into BMP one is also presented.
4.Optimization of isolation and culture conditions of rabbit bone marrow mesenchymal stem cells
Junren HE ; Ziquan YANG ; Bing LI ; Xiaochun WEI
Chinese Journal of Tissue Engineering Research 2007;0(43):-
BACKGROUND: Different isolation and culture methods for bone marrow mesenchymal stem cell (BMSCs) will result in varying cell activity and purity, which will influence repair effect of tissue engineering. OBJECTIVE: To investigate the optimized methods of isolation and culture of BMSCs in vitro and validate the efficacy of cell acquisition. DESIGN, TIME AND SETTING: In vitro cytology trial was performed at the laboratory of Department of Orthopedics, Second Hospital of Shanxi Medical University from October to December 2007. MATERIALS: Two 3-month-old New Zealand rabbits were provided by Shanxi Institute of Livestock. METHODS: Lymphocyte isolation solution was added to DMEM culture solution containing fresh bone marrow. BMSCs were obtained and purified by gradient centrifuge and adhesion culture in vitro. Non-attached cells were moved to new culture flask every 8 hours. The solution was changed firstly after 5 days of culture. Trypsinization was conducted at cell confluence of 90%. The first, third and fifth passages of cells were harvested. MAIN OUTCOME MEASURES: The morphology of BMSCs was observed with phase contrast microscope; the growth curve was drawn to determine doubling time. The percentage of the wel1 growth P2 cells were identified by CD44 staining by flow cytometry. RESULTS: Primary cultured BMSCs were oval, spindle-shaped or polygonal, and adhered to plastic surface within 48 hours, exhibiting spindle-shaped or polygonal with clear nucleus, and reached 90% confluence within 14 days. The first, third and fifth passages of BMSCs showed S-type, and were in latent phase at 1-3 days, in log phase 3 days later, and cells came into platform phase at 7-8 days. The mean doubling time was 24.22 hours. CD44-positive cells were found in the second passage, with positive rate of 93.0%. CONCLUSION: BMSCs are easily isolated and cultured in vitro with good growth and high purity by gradient centrifuge and adhesion culture with lymphocyte isolation solution.
5.Maternal serum lipid at 36-42 weeks′ gestation and their relationship to newborn weight in pregnant women with gestational diabetes mellitus and type 1 and type 2 diabetes mellitus
Bing HE ; Shuqin LI ; Wei WANG ; Ping HAN ;
Chinese Journal of Obstetrics and Gynecology 2000;0(10):-
Objective To investigate the levels of serum lipid at 36 42 weeks' gestation and their relationship to newborn weight in pregnant women with gestational diabetes mellitus (GDM) and type 1 and type 2 diabetes mellitus (DM) Methods This study included 30 normal pregnant women, 40 patients with GDM, 30 patients with type 1 DM and 30 with type 2 DM Subjects with complications including hypertension or thyreogenic diseases were excluded Fasting serum triglyceride, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol were measured by enzymatic method Results (1) Serum triglyceride levels in pregnant women with GDM, type 1 DM, type 2 DM and normal controls were (4 0?1 4) mmol/L ,(2 9?0 8)mmol/L, (4 1?2 5)mmol/L and(2 7?0 9)mmol/L respectively; the total cholesterol levels were(6 5?3 0)mmol/L,(6 2?2 8) mmol/L,(6 4?3 2)mmol/L and(6 0?3 0)mmol/L respectively; low density lipoprotein cholesterol levels were(3 2?1 3)mmol/L, (3 2?1 3)mmol/L,(3 3?1 1) mmol/L and(3 2?1 0)mmol/L respectively; high density lipoprotein cholesterol levels were(1 6?0 5)mmol/L, (1 4?0 5)mmol/L, (1 5?0 4)mmol/L and(1 6?0 4)mmol/L respectively; the newborn weights were(4108?544)g,(3323?457)g,(4111?263)g, and(3463?516)g respectively Triglyceride levels in pregnant women with GDM and type 2 DM were significantly higher than in normal controls and type 1 DM patients ( P
6.Integrated assessment of middle ear dysfunction in cleft palate patients and optimization of therapeutic schedule.
Wenrong JIANG ; Tao HE ; Qian ZHENG ; Wei ZHENG ; Bing SHI ; Chao YANG ; Chenghao LI
West China Journal of Stomatology 2015;33(3):255-258
OBJECTIVETo explore evaluation strategies for middle ear dysfunction in cleft palate patients, to optimize the diagnosis and treatment of this dysfunction, and ultimately to improve the comprehensive treatment of cleft palate.
METHODSThe relationship among abnormal tympanic types (B, C, and Anomaly), effusion rate, tympanic pressure, and hearing loss were analyzed. We collected relevant information on 469 ears of cleft palate patients and traced one-year longitudinal changes in the tympana of 124 ears from 62 patients with both cleft lip and cleft palate.
RESULTSThe effusion rates of cleft palate patients with type B, type C, and type Anomaly were 50.3% (97/193), 34.8% (8/23), and 20.9% (53/253), respectively. The tympanic pressure of the ears with and without effusion showed no significant difference (P>0.05). The hearing loss in type B cleft palate patients with middle ear effusion was worse than that in patients without effusion (P=0.001). However, the hearing loss in type Anomaly showed no difference (P>0.05). The constituent ratio of each tympanic type remained constant during the period between cheiloplasty and palatoplasty for cleft lip and palate patients (P>0.05).
CONCLUSIONCleft palate patients of all tympanic types may all suffer from middle ear effusion at different rates. Examination by centesis is suggested for ears with abnormal tympanic types. Early aggressive therapy is essential for type B cleft palate patients with middle ear effusion to avoid hearing loss. However, catheterization may be not necessary for type Anomaly patients, and conservative observation should be performed instead. Myringotomy with grommet insertion during palatoplasty does not delay treatment timing for patients with both cleft lip and cleft palateg.
Cleft Lip ; Cleft Palate ; Ear, Middle ; physiology ; Humans ; Middle Ear Ventilation ; Otitis Media with Effusion ; diagnosis ; epidemiology
7.Entity of acupoint: kinetic changes of acupoints in histocytochemistry.
Wei HE ; Meiling WU ; Xiang-hong JING ; Wanzhu BAI ; Bing ZHU ; Xiaochun YU
Chinese Acupuncture & Moxibustion 2015;35(11):1181-1186
The present study aims to investigate the kinetic histocytochemical changes of acupoints in different condition. The expression of tryptase (+) mast cells, histamine (HA) , serotonin (5-HT) and nociceptive neuropeptides including calcitonin gene-related peptide (CGRP) and substance P (SP) were observed by immunohistochemistry combined with confocal technology. Mast cells were labeled with anti-mast cell tryptase antibody and simultaneously with HA or 5-HT primary antibodies to observe their co-expression. The results showed that: (1) SP and CGRP were expressed more highly on the cutaneous nerve fibers of "Hegu" (LI 4) after acupuncture stimulation than that of the control. Mast cells aggregated in close proximity to the blood vessels in intra-epidermis and dermis, and some of them with degranulation in the lower dermis and subcutaneous tissue of "Hegu" (LI 4). Both mast cells and their granules appeared with HA (+) and 5-HT (+) expression at stimulated LI 4 sites, while a few intact mast cells with a little expression of 5-HT and HA were distributed in areas of non-stimulated Ll 4. (2) The acupoints in different locations such as Baihui (GV 20), Weishu (BL 21), Zhongwan (CV 12) and LI 4 had the same constituent but the contents were different. (3) The histocytochemical responses of acupoints sensitized by the Gastric mucosa injury (GMI) were also investigated. GMI resulted in neurogenic plasma extravasation by Evans Blue (EB) in the skin of the acupoints over the back and abdomen, which mostly occurred in the T9-T11 dermatomere. The EB extravasation dots just like acupoints sensitization appeared after GMI and disappeared gradually during the natural self-recovery of the gastric mucosa. More SP and CGRP positive nerve fibers were distributed in EB dots than in regions beside EB dots and in the control, mostly distributed in the nerve fibers around both the vessels and root of hair follicle. Mast cells also aggregated and degranulated to release algogenic substances of 5-HT and HA around the vessels in areas of the EB dots. Collectively the acupoints displayed the same histocytochemical responses due to either acupuncture stimulation or GMI. This may potentially be the histocytochemical basis in the local acupoints and acupoints displayed kinetic changes in different condition.
Acupuncture Points
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Acupuncture Therapy
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Animals
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Calcitonin Gene-Related Peptide
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metabolism
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Gastric Mucosa
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chemistry
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metabolism
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Histocytochemistry
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Humans
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Mast Cells
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chemistry
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metabolism
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Mice
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Rats
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Serotonin
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metabolism
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Skin
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chemistry
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metabolism
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Substance P
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metabolism
8.ZHU Lian--the founder of Chinese acupuncture-moxibustion scientific research.
Yang-Shuai SU ; Bing LIU ; Xiang-Hong JING ; Wei HE ; Xiao-Yu WANG ; Li-Jian ZHANG
Chinese Acupuncture & Moxibustion 2014;34(12):1221-1224
This article discussed ZHU Lian's contributions to acupuncture-moxibustion scientific research from three aspects: building the scientific thought of "new acupuncture-moxibustion", constructing the first domestic acupuncture-moxibustion institution and opening the door to modern acupuncture-moxibustion scientific research. ZHU Lian's visionary thought of "new acupuncture-moxibustion" has influenced the following researchers till now. She established the acupuncture-Moxibustion therapeutic institute affiliated to the Ministry of Health, set up the acupuncture-Moxibustion research platforms and teams and made research cooperation. She firstly carried out acupuncture-Moxibustion clinical and basic scientific research, which started the acupuncture-Moxi- bustion scientific research in China. ZHU Lian is the Pioneer of Chinese acupuncture-Moxibustion scientific research.
Acupuncture
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education
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Acupuncture Therapy
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history
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China
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History, 20th Century
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Humans
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Moxibustion
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history
9.Pseudoangiomatous variant of spindle cell lipoma: report of a case.
Bing-wei DONG ; Xiao-yan MA ; Hui-nü HE ; Fen-juan ZHANG
Chinese Journal of Pathology 2013;42(5):343-344
12E7 Antigen
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Antigens, CD
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metabolism
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Antigens, CD34
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metabolism
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Cell Adhesion Molecules
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metabolism
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Diagnosis, Differential
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Head and Neck Neoplasms
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metabolism
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pathology
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Hemangioma
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pathology
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Humans
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Lipoma
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metabolism
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pathology
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Liposarcoma, Myxoid
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pathology
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Male
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Middle Aged
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S100 Proteins
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metabolism
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Soft Tissue Neoplasms
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metabolism
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pathology
10.Clinical research of LMWH in therapy of women with RSA pregnant by ART
Lin ZOU ; Honghua HE ; Xiaoyan PANG ; Cailing PENG ; Bing WEI ; Lihua XU
Journal of Medical Postgraduates 2017;30(4):399-404
Objective There are few studies on the application of low molecular weight heparin (LMWH) in the therapy of women with recurrent spontaneous abortion (RSA) pregnant by assisted reproductive technology (ART).The article aimed to explore the clinical research of LMWH in therapy of women with RSA pregnant by ART.Methods 126 women with RSA pregnant by ART were enrolled and they were diagnosed and treated in Reproductive Medicine Center in the Affiliated Hospital of Guangdong Medical University from January 2010 to February 2016.According to the patient′s agreement on LMWH treatment, 60 patients in agreement with LMWH treatment were divided into 2 groups: LMWH+IUI group(n=30) and LMWH+ IVF-ET group(n=30).66 patients in disagreement with LMWH treatment were also divided into 2 groups: IUI group(n=32) and IVF-ET group(n=34).Comparison was made in patients from 4 groups concerning pregnancy success rate, live birth rate, pregnancy time involving RSA and incidence of pregnancy complications.At the same time, the occurrence of adverse reactions during the use of LMWH was also observed.Results The pregnancy time involving RSA in LMWH+IUI group significantly increased compared with IUI group([82.67±9.10]d vs [48.17±8.68]d)(P<0.05).The pregnancy success rate and live birth rate in LMWH+ IVF-ET group were both higher than those of IVF-ET group (66.7% vs 35.29%, 85.00% vs 50.00%)(P<0.05) and significant difference was also found in the incidence of RSA, the pregnancy time involving RSA, and the morbidity of gestational hypertension between groups(P<0.05).The results of D2D at 4 weeks of pregnancy in LMWH+IUI group([0.65±0.07]mg/L) and LMWH+ IVF-ET group([0.625±0.06]mg/L) were lower than those of LMWH group ([0.76±0.12]mg/L) and LMWH group([0.77±0.06]mg/L).The result of D2D at 6 weeks of pregnancy in LMWH+IUI group was lower than those of IUI group and IVF-ET group, and D2D in LMWH+ IVF-ET group was lower compared with IVF-ET group(P<0.05).The results of D2D in all the four groups increased with the pregnancy weeks(P<0.05).The prothrombin time(PT) at 4 weeks of pregnancy in LMWH+IUI group, IUI group and LMWH+ IVF-ET group ([12.53±0.38]s, [12.38±0.65]s, [12.47±0.58]s) was significantly higher at pre-pregnancy([12.33±0.52]s, [12.30±0.68]s, [12.22±0.64]s) and 6 weeks of pregnancy([12.13±0.62]s, [12.05±0.60]s, [12.03±0.54]s) (P<0.05).Among 60 cases treated with LMWH, small area ecchymoma were found in 11 cases and the incidence was 18.33%(11/60), only two cases reported with uncomfortable light pain in the location of subcutaneous injection.Conclusion Low-dose LMWH is safe and effective in the therapy of pregnant women with RSA through ART.