Cellulose, Oxidized ; therapeutic use ; Child, Preschool ; Esophagus ; Foreign Bodies ; pathology ; therapy ; Hemorrhage ; therapy ; Humans ; Infant ; Male

Objective: To study the influence of different amino group introductions on the antiplatelet aggregation activities of pyridazinone derivatives. Methods: The target compounds were designed and synthesized by inletting different substitution amino groups using acetyl fragment as the connecting segment. Born method was used for preliminary pharmacological test in vitro. Results: Ten target compounds were designed and synthesized; 8 of them were reported firstly and all of them were confirmed by 1HNMR spectra. The results of preliminary pharmacological test showed that all the target compounds exhibited potent antiplatelet aggregation activity. The antiaggregation activities of compounds (6f), (6g), (6h) and (6j) were stong; compounds (6g) and (6j) showed a 5-time higher activity than 6-[4-(pyridin-4-yl-amino) phenyl]-4, 5-dihydro-3 (2H)-pyridazinone (MCI-154). Conclusion: Inletting different substitution amino groups can enhance the antiplatelet aggregation activity of the compounds.

Objective To apply the virtual reality training in clinical education of rehabilitation therapy. Methods 26 undergraduate students majored in rehabilitation therapy were randomly divided into 2 groups during clinical education of physiotherapy. The experimental group (n=14) received virtual reality training first, and then with routine teaching, while the control group (n=12) learnt with routine teaching only. Self-assessment and physiotherapy test were conducted after training. Results The experimental group reported more interested, initiative and positive in training than those of the control group. The experimental group achieved higher scores in practical tests than the control group (P<0.05), while no significant difference in the theoretical examination between the groups. Conclusion Application of virtual reality training may improve the teaching of physiotherapy.

Objective To systematically evaluate the clinical effectiveness and safety of Breviscapine injection combined with conventional therapy in the treatment of unstable angina pectoris (UAP).Methods The literature were systematicly retrieved about the randomized controlled trials (RCTs) of Breviscapine injection for UAP. The Cochrane Risk of Bias Assessment Table was used to evaluate the methodological quality of the RCTs and then the data were extracted and meta-analysed by RevMan 5.3 software.Results A total of 15 RCTs with 1202 participants were included. In the meta-analysis, the combination of Breviscapine injection and conventional therapy in the treatment of UAP can achieve better angina pectoris curative effect (Z=7.74,P<0.01), theRR(95% CI) was 1.23 (1.17,1.30), and the electrocardiogram curative effect (Z=6.26,P<0.01), the RR(95% CI) was 1.32(1.21, 1.44). Furthermore, Breviscapine injection can improve hemorheologic parameters. And there was no major or serious adverse drug event.Conclusions The Breviscapine injection combined with conventional therapy shows good therapeutic effect on UAP.

Objective:To evaluate the relationship between breast reconstruction and postoperative complications by meta-analysis.Methods:Through a defined search strategy, related literature was collected in databases from PubMed, MEDLINE, EMBASE, Cochrane, CNKI, Wanfang Database and VIP Database, from January 1990 to November 2017. Data were extracted and each merged data was analyzed using RevMan 5.3 software. The postoperative complications between transverse rectus abdominis musculocutaneous-flap (TRAM) and deep inferior epigastric perforatorflap (DIEP), TRAM and latissimus dorsiflap (LDF), LDF and LDF+ prosthesis, LDF+ prosthesis and simple prosthesis implantation were compared.Results:Twenty-nine papers met inclusion criteria of our study. The Meta-analysis results showed that the risk of local flap necrosis, seroma, infection, fat liquefaction, abdominal wall hernia and abdominal bulging in TRAM group were higher than those in DIEP group, and the differences were significant. There was no significant difference in total flap necrosis, wound dehiscence, venous congestion between the two groups; the risk of total flap necrosis, wound dehiscence and infection in TRAM group were higher than those in LDF group, and the differences were significant. The risk of seroma in TRAM group was lower than that in LDF group, and the differences were significant. There was no significant difference in fat liquefaction between the two groups; there was no significant difference in the postoperative complications between LDF group and LDF+ prosthesis group; the risk of seroma in LDF+ prosthetic group was higher than that in prosthetic group, and the differences was significant. The risk of prosthetic capsular contracture and prosthesis displacement in LDF+ prosthetic group were lower than those in prosthetic group, and the differences were significant. There was no significant difference in prosthesis exposure and infection between the two groups.Conclusions:DIEP has most of the advantages of TRAM and fewer complications. It plays an important role in breast reconstruction in the future. We should make a choice of breast reconstruction methods according to the patient＇s conditions as far as possible in clinical practice.

Objective To evaluate the cefoxitin screening test in detecting(MRSA)with the real-time fluorescent quantitative polymerase chain reaction(RT-PCR)for detecting mecA gene as the golden standard.Methods Staphylococcus aureus strains iso-lated from various bacterial infection specimens were collected,which were amplified by RT-PCR and detected by the cefoxitin screening test respectively.The results were compared.Results In 121strains of MRSA,66 strains carrying mecA gene were detec-ted by RT-PCR(P >0.05),accounting for 54.55%(66/121).There was no statsitcal difference in the detection results between the cefoxitin screening test and RT-PCR.The sensitivity of the cefoxitin screening test was 93.93%,the specificity was 81.82%,the positive predictive value was 84.93% and negative predictive value was 91.83%.Conclusion The RT-PCR technique can accurate-ly and rapidly detect MRSA.

Tamoxifen and letrozole are two of the most effective pharmaceuticals mainly used in hormonal dependent) breast cancer therapy. A trace analytical method using ultra performance liquid chromatography/tandem mass spectrometry(UPLC-MS/MS) was developed to simultaneously detect these two drugs in both influent) and effluent of municipal sewage treatment plant. Sewage samples were passed through an Oasis HLB cartridge, then an amino solid phase extraction cartridge was connected with Oasis HLB cartridge. Target compounds) was firstly eluted with 6 mL methanol and then 3 mL methanol was used to elute the amino solid phase extraction cartridge, collected and pooled the elutants. After concentration, target compounds were separated) on a BEH C_(18)) column using a gradient elution profile with a mobile phase consisting of 0.1% formic acid aqueous) solution and acetonitrile and detected by an electro spray ionization tandem mass spectrometry with multiple reactions monitoring(MRM). Satisfactory linearity(R~2>0.997) was obtained over the range of 1.0-100 μg/L) and 0.1-100 μg/L for tamoxifen and letrozole, respectively, with limits of quantification(LOQ) of 1.0 and 0.1 ng/L. Mean recoveries of two target compounds(spiked in sewage samples at three concentration levels) ranged from 68.8% to 103.0%, with relative standard deviations(RSD) less than 15%. This method can be applied for the analysis of target drugs sewage samples.

7% (OR=4.640,95%CI=1.064 to 20.239,P=0.041) were the high risk factors for myocardial ischemia in patients with type 2 diabetes.Conclusion Poor control of the 4 factors is the high risk factor for myocardial ischemia in patients with type 2 diabetes.Overall and effective control of such risk factors can decrease the incidence of myocardial ischemia and improve its treatment.

bjective: To study the expression of Slit protein and vascular endothelial cell growth factor(VEGF) in carcinogenesis of human oral mucosa and to investigate the relationship between the Slit and angiogenesis.Methods: The expression of Slit protein and VEGF was detected using immunohistochemical method,microvessel density (MVD) was counted following immunostaining with anti-vWF antibody in 40 cases of human oral squamous cell carcinoma(OSCC),18 cases of simple hyperplasia,20 cases of dysplasia,20 cases of carcinoma in situ and 19 cases of normal oral mucosa(NOM).Results The positive expression of Slit was detected in 34 cases of OSCC,4 of simple hyperplasia,7 of dysplasia,9 of carcinoma in situ and 1 of NOM(P

Objective:To investigate the effect of Ku70 silencing on the expression of apoptosis-related genes in human NSLC cell line,A549 and drug-resistant A549DDP.Methods:Expression of Ku70mRNA in human A549 cells was examined by RT-PCR.Expression of apoptosis-related genes was examined by PCR-Array assay.Results: The Ku70mRNA was higher in A549DDP cells than in A549 cells.The expression of BAX,TP53 and TP73 was significantly increased whereas the expression of TNFRSF1A and BFAR decreased in siRNA-Ku70 A549DDP cells when compared to those of A549DDP cells.Conclsion:The amount of Ku70 mRNA is higher in A549DDP cells,suggesting that Ku70 is important for drug-resistance; Silencing Ku70 might reverse the drug-resistance in A549DDP cells through regulation of expression of apoptofic genes.