Objective To summarize the clinical experience of laparoscopic cholecystectomy (LC) in the treatment of acute cholecystitis. Methods A total of 93 cases of acute cholecystitis treated by LC from May 2003 to May 2005 was retrospectively reviewed, including 15 cases of preoperative endoscopic retrograde cholangiopancreatography (ERCP) combined with endoscopic sphincterotomy (EST) for common bile duct stones and 6 cases of intraoperative cholangiography. The LC was performed within 48 hours after admission. Results The LC was successfully completed in 91 cases (97.8%), whereas a conversion to open surgery was required in 2 cases (2.2%). The operation time was 35~160 min (mean, 65 min). Postoperatively, biliary leakage occurred in 3 cases (3.2%) and residual stones in the common bile duct were found in 3 cases (3.2%), which were all cured by open surgery combined with ERCP, EST, and endoscopic nasobiliary drainage (ENBD). No iatrogenic injuries happened. Conclusions With proper selection of ERCP and EST, LC for the treatment of acute cholecystitis is feasible and safe. But the incidence of conversions and complications may be high.

Aged ; Animals ; China ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Osteoporosis ; drug therapy ; Phytotherapy ; Stroke ; drug therapy ; Yang Deficiency ; drug therapy ; Yin Deficiency ; drug therapy

Data Mining ; Humans ; Medicine, Chinese Traditional ; Stomach Diseases ; therapy

Administration, Oral ; Animals ; Mice ; Nanoparticles ; toxicity ; Particle Size ; Titanium ; toxicity ; Toxicity Tests, Acute

0.05).EBV-specific IgM was detected more frequently in patients than that in controls(P

OBJECTIVE: To establish the determination method of the related substances in ibuprofen raw materials, tablets and capsules. METHODS: High performance liquid chromatography (HPLC) was used for the determination of 2-(4-isobutyl phenyl) propanoic acid, 4-isobutyl benzoic acid, sodium methyl hydroxybenzoate(provided by national institutes for food and drug control), 4′-isobutyl acetophenone (known impurities of JP), the relative substance A, J, N (known impurities of EP), and the unknown impurities. A Kromasil C18 column (4.6 mm × 250 mm, 5 μm) was used as the analytical column, acetonitrile-water (pH adjusted to 3.0 by phosphoric acid) (45:55) were used as the mobile phase, the flowing rate was 1.0 mL · min-1, the detection wavelength was set at 254 nm, and the column temperature was 35°C. RESULTS: The relative substances detected in ibuprofen raw materials and its preparations were mainly 4-isobutyl benzoic acid and 4′-isobutyl acetophenone. The contents of unknown impurities were also high in the preparations of some manufacturers. CONCLUSION: The method can be used for the analysis of the related substances in ibuprofen raw materials and its preparations.

Objectives:This study was designed to establish the modified method of cardiomyocytes culture in human fetal hearts. Methods:The human fetal heart cells of ventricular muscle were isolated by 0.2% trypsin and 0.1% collagenase and cultured primarily and passaged in Iscove's modified Dulbecco's medium(IMDM) in vitro by means of differential attachment technique.The changes in morphology,ultrastructure,viability,immunocytochemistry antibody staining and immunofluorescence antibody staining of human fetal heart cells were studied in culture. Results:The ratio of viable cells was 99% identified by trypan blue staining.The ratio of attachment cells was 95% after 24 h in culture.The cultured human fetal heart cells were roundness shaped,rod shaped,shuttle shaped,ellipse shaped,star shaped and bifurcate shaped with spontaneous contractility.The myocardial actin and myoglobin are identified in the cultured cells by immunocytochemistry antibody staining and immunofluorescence antibody staining.The ultrastructure of cells was similar to that of the cardiac tissue in vivo by electron microscopy.Human fetal heart cells after 20 d of primary culturing and after 5 d of passaged culturing were growed well. Conclusions:The method for isolating and culturing human fetal heart cells is successful and reliable.This model provides an effective experimental mothod for studying the mechanism of myocardial injury.

Delightful achievements have been obtained in forestry genetic breeding since the application of transgenic technology in this field during the past 20 years. Field trials of some genetic modified (GM) trees have been carried out, and some GM trees have been commercialized. Meanwhile, the risks of ecological safety caused by GM trees have raised attention in the public gradually. These issues mainly include the horizontal transfer and vertical flow of foreign genes, and the potential effects on insects, soil ecosystems and virus. The current status of field trials, commercial applications and the potential ecological risks of GM trees were summarized. Then the prospects of GM trees were also presented.

To investigate the problem and solution strategy of the clinical medical education of foreign students by applying the simulation teaching in Ob/Gyn clinical clerkship of foreign students. It demonstrates that combing the simulation teaching for diagnosis and treatment with the heuristic teaching can help the student overcome language barrier, the lack of basic theoretical knowledge and shortage of clinical teaching resources and it's of great significance in clinical medical education of foreign students.

BACKGROUND:Coracoclavicular ligament reconstruction with transclavicular-transcoracoid driling is an effective surgical technique to treat acromioclavicular dislocation. A good driling in the clavicle leads to a perfect bony tunnel and a good surgery. OBJECTIVE: To observe the effects of different driling positions of the clavicle on the location of bony tunnels in coracoclavicular ligament reconstruction. METHODS:Sixty three-dimensional digital models of the clavicle and coracoid process were constructed by Mimics13.0. Virtual transclavicular-transcoracoid bony tunnels were established according to different surgical planes with different driling positions in the clavicle. Parameters of these bony tunnels were measured, and the safety was evaluated. Option 1: The driling was made 30 mm distal to the clavicle, located in the center of the front and rear edges of the clavicle surface. Option 2: The driling was made 40 mm distal to the clavicle, located in the center of the front and rear edges of the clavicle surface. Option 3: The driling was made at the straight line of tapered nodule tip and the midpoint of the base of the coracoid process, located at the rear edge of the clavicle upper surface. RESULTS AND CONCLUSION: Bony tunnels in option 1 were extremely on the inside of the coracoid. Bony tunnels in options 1 and 2 were not in the center of clavicle. Bony tunnels in option 3 were in the center of both clavicle and coracoid. The method of locating the driling position with a certain distance to the distal clavicle leads to different results in man’s and woman’s models. To ensure that the bony tunnel can pass through the center of clavicle and coracoid, it is suggested to dril at the straight line of tapered nodule tip and the midpoint of the base of the coracoid process and nearby the rear edge of the clavicle upper surface.