Objective To study the role of protein kinase B(PKB) in gastric cancer BGC-823 cells treated by TPA. Methods Flow cytomety(FAC) and fluorescence microscope were used to detect the effect of TPA on gastric cancer BGC-823 cells,after cells were treated by BrdU and stained by DAPI,respectively.The effect of TPA on the expression level of PKB protein and its phosphorylation of Ser 473 and Thr 308 were investigated by Western blotting.When nuclear and cytoplasmic protein fractions were prepared through lysis of cell and centrifugation,the effect of TPA on the expression level of PKB protein and its phosphorylation of Ser 473 in cytoplasm and nuclei were detected by Western blotting.Meanwhile,the effect of TPA on the distribution of PKB was observed under laser-scanning confocal microscope with immunofluorescence technique. Results TPA induced the apoptosis of gastric cancer BGC-823 cells.It also induced the inhibition of PKB protein expression in a TPA concentration-dependent and time-dependent manner in gastric cancer BGC-823 cells,regardless of dephosphorylation of PP2A.TPA inhibited the phosphorylation of PKB at Ser 473,but it did not affect its phosphorylation at Thr308.TPA only attenuated the expression level of PKB and its phosphorylation of Ser 473 in nuclei,whereas it did not change the distribution of PKB in BGC-823 cells.Conclusion These data suggest that the inhibition of PKB plays an important role in mediating the effect of TPA in gastric cell line.Apoptosis induced by TPA is partly due to the inhibition of PKB and its phosphorylation of Ser 473 in nuclei.

hydroxysteroid dehydrogenase (11? HSD) catalyzes the interconversion of cortisol with its inactive metabolite cortisone. The congenital deficiency of 11? HSD2 induce hypertension and hypokalemia. This disorder is called "Apparent Mineralocorticoid Excess(AME)". Glycyrrhizic acid and other endo and xenobiotics have been found to inhibit the activity of 11? HSD and cause excess mineralocorticoid effects that is similar to AME. The decrease in 11? HSD activity is related with the acquired and congenital hypertention.

Nowadays,aging is the general trend of population development in the world.Type 2 diabetes is one of the most common chronic diseases in the elderly.Because of the atypical symptoms and signs,accompaniment of chronic cardiovascular disease and vulnerability to low blood glucose in the elder patients with diabetes,we should to make safe,effective,and individualized therapeutic programs for them.This paper will review the current prevalence,characteristics,and oral drug selection among elderly individuals with T2DM.

Objective:To explore the correlation among serum uric acid (UA) level and severity of coronary heart disease (CHD) ,adverse cardiovascular events .Methods :Clinical data of 403 patients undergoing coronary angiog-raphy in our hospital were retrospectively analyzed .They were divided into CHD group [n=308 ,including 137 cases with stable angina pectoris (SAP) and 171 cases with acute coronary syndrome (ACS)] and normal control group (n=95 ,non-CHD patients) .UA level was compared among all groups and its correlation with CHD severity was fur-ther analyzed .According to UA level ,CHD patients were divided into low UA group (n=147) and high UA group (n=161) ,after 12-month follow-up ,incidence rate of major adverse cardiovascular events (MACE) uring follow-up was compared between two groups .Results:Serum UA level of ACS group was significantly higher than that of nor-mal control group [ (318.44 ± 69.07)μmol/L vs .(295.38 ± 80.08)μmol/L ,P=0.003];along with number of dis-eased coronary vessels increased and CHD severity aggravated (Gensini score rose ) ,serum UA level showed an in-creasing trend [single-branch (316.58 ± 95.27 ) μmol/L vs . double-branch (335.26 ± 43.26 ) μmol/L vs . multi-branch (346.53 ± 86.74) μmol/L ;low score group (312.42 ± 48.26) μmol/L vs .middle score group (346.58 ± 47.36)μmol/L vs .high score group (363.84 ± 54.68)μmol/L , P<0.05 or <0.01] .Pearson correlation analysis indicated that serum UA level was positively correlated with Gensini score (r=0.583 ,P<0.05);MACE incidence rate in high UA group was significantly higher than that of low UA group (58.38% vs .36.24% , P=0.012) .Con-clusion:Serum uric acid level is positively correlated with severity of coronary heart disease ,which may be an inde-pendent risk factor for coronary heart disease .

Objective To evaluate the value of transvaginal two-dimensional ultrasound(2D-TVS)and transvaginal three-dimensional ultrasound (3D-TVS) in the diagnosis of intrauterine lesions with abnormal uterine bleeding.Methods Intrauterine lesions for 171 patients underwent 2D-TVS followed by 3D-TVS in the same session.Within 10 days of the sonographic session,each patient underwent pathologic histology examination after hysteroscopy examination or surgery therapy,which was considered the reference standard.For each of the two imaging methods,sensitivity,specificity,and accuracy were calculated.Results The results of 2D-TVS and 3D-TVS had good agreement with pathological results in endometrial hyperplasia,endometrial polyps,uterus submucous myoma and endometrial cancer.For 2D-TVS,sensitivity was 84.5％,71.4％,86.8％,66.7％,and specificity was 86.7％,90.7％,97.0％,98.7％,respectively; For 3D-TVS,sensitivity was 91.4％,87.3％,92.1％,83.3％,and specificity was 93.8％,96.3 ％,97.7 ％,98.7 ％,respectively.The diagnose accordance rate of the 3D-TVS for intrauterine lesions was statistically higher than that of the 2D-TVS (89.5％ vs 78.9％,P ＜0.05).Conclusions The sensitivity and diagnose accordance rate of 3D-TVS was higher than that by 2D-TVS,3D-TVS plays an important role in the diagnosis and differential diagnosis of intrauterine lesions.

Objective To explore the clinical value of the insulin pump(CSⅡ) and multiple subcutaneous insulin intensive treat-ment(MDI) for patients with type 2 diabetes and stroke .Methods A total of 80 patients with type 2 diabetes and stroke in Weifang Community Health Service Center from April 2011 to May 2014 were selected as subjects and divided into experimental group and control group according to random number table method ,with 40 cases in each group .Patients in the experimental group were trea-ted with CSⅡ ,patients in the control group were treated with MDI .Glucose level ,therapeutic time ,the number of low blood sugar , insulin dosage ,National Institute of Health Stroke Scale(NIHSS) score ,insulin secretion index(IS) ,insulin resistance index(IR) , hospital costs were compared before and after treatment in the two groups .Results The glucose levels ,therapeutic time ,the num-ber of low blood sugar ,insulin dosage ,NIHSS score ,IS ,IR in the two groups before and after breakfast ,before and after lunch ,be-fore dinner ,after dinner 2 hours before treatment had no significant differences(P>0 .05) ,the glucose levels ,therapeutic time ,the number of low blood sugar ,insulin dosage ,NIHSS score ,IS ,IR after treatment in both groups were significantly decreased at these time points ,but those indicators in the experimental group decreased more significantly ,and had significant differences with those of the control group(P<0 .05) .The cost of hospitalization of patients in the experimental group was significant lower than that in the control group(P<0 .05) .Conclusion CSⅡ treatment for type 2 diabetes and stroke patients ,not only control glucose level well ,re-duce the number of low blood glucose and insulin dosage ,significantly improved IS and IR ,reduce hospital costs ,and also could sig-nificantly improve the neurological disorders of consciousness ,which is an ideal treatment method compared with MDI .

BACKGROUND:An increasing number of studies have shown that mesenchymal stem cels have the potential to treat acute kidney injury. Umbilical cord-derived mesenchymal stem cels have general characteristics of stem cels and many advantages, such as easy to isolate and culture, in vitrofast amplification, low immunogenicity and no ethical problems, which have garnered increasing attentions. OBJECTIVE:To study the repairing effects of human umbilical cord-derived mesenchymal stem cels on acute kidney injury in rats. METHODS: Thirty rats were randomized into three groups: a normal control group, a model group and a cel transplantation group. Rats in the model and cel transplantation were subjected to clamping the renal pedicles for 45 minutes, and then injected 1 mL of DAPI-labeled umbilical cord-derived mesenchymal stem cels or 1 mL of saline via the tail vein. In the normal control group, the kidney was only exposed with no treatment. At 7 days after treatment, the rats were kiled to take left kidney tissues for pathological observation under light microscope and right kidney for observation of DAPI-positive cel counting. Automatic biochemical analyzer was used to detect serum creatinine and urea ammonia levels. RESULTS AND CONCLUSION: Compared with the model group, the levels of serum creatinine and urea ammonia were significantly lower in the cel transplantation group (P < 0.05), suggesting that human umbilical cord-derived mesenchymal stem cels can improve the kidney function to a certain extent. Pathological findings showed that the pathological damage was improved more remarkably in the cel transplantation group than the model group, and the tubular necrosis index decreased significantly in the cel transplantation group. At 7 days after cel transplantation, blue fluorescent cels were scattered on renal tissue frozen sections. These results indicate that human umbilical cord-derived mesenchymal stem cels can migrate to the injured tubular epithelial tissues, and promote the repair of the injured kidney.

Depression is a worldwide neuropsychiatric disorder. Currently most preclinical and clinical studies of depression focus on monoaminergic system. However, there is growing evidence which suggests that glutamatergic system plays a critical role in the pathophysiology of depression. This review focuses on the de-velopment of new antidepressants that target glutamatergic sys-tem, summarizes the current mechanisms of antidepressants, and also highlights new insights to the pathophysiology of depression.

Objective: To investigate the effects of nursing interventions on mood in patients with coronary heart disease, who performed after percutaneous coronary intervention (PCI). Methods:Retrospective analysis of all patients who Performed PCI in Shunyi District Hospital in 2012. Results: All patients during in the examination room underwent psychological care and transporting in progress of preoperative, intraoperative, and postoperative, which reduced surgical complications and adverse reactions, meanwhile increased surgical success rate. Conclusion:Psychological care and intervention can effectively reduce the feeling of discomfort and anxiety in patients during surgery of coronary intervention, which is an important part of the modernphysical-psychological-socialmedical model.

OBJECTIVE:To establish a RP-HPLC method for the determination of Chlorogenic acid in Pneumonia mixture. METHODS: The separation of samples was performed on a column of Spheri-5 RP-C18 (250 mm?4.6 mm,5 ?m). The mobile phase consisted of acetonitrile-0.4% phosphate (8.5∶91.5) with a flow rate of 1.0 mL?min-1.The detection wavelength was set at 328 nm. RESULTS: The calibration curve was linear in the range of 5.00～100.00 ?g?mL-1 for Chlorogenic acid(r=0.999 9).The average recovery was 98.15%,(RSD=3.68%,n=6).CONCLUSION: The method was rapid, simple and accurate, and it can be used for the quality control of Pneumonia mixture.