Kidney theory is an important figure in traditional Chinese medicine (TCM), it proposes that the kidney is the root of life, in which stores congenital essence and energy of human body that promotes reproduction, growth and development. The facts that body development begins from oosperm have long been elucidated in modern medicine and life science. Furthermore, oosperm is a totipotent stem cell. The totipotent stem cell with its derived embryonic stem cell (ESC) and stem cell constitute the source of growth, development and tissue regeneration. It is rational to comprehend ESC by means of the Kidney theory of TCM, investigate the effect of Kidney-nourishing on the activities of ESC, such as proliferation, aging and apoptosis, and on its related genes regarding transcription, expression and functional regulation. Our works have suggested that classical Kidney-yin-nourishing herb formula Zuo-gui-wan could inhibit the ESC differentiation and apoptosis, stimulate proliferation, and promote the process of cell cycle. These observations coincide with up-regulate expression of Wnt and Oct3/4, and down-regulate expression of P16INK4a. At the same time, classical Kidney-yang-nourishing herb formula You-gui-wan could inhibit ESC apoptosis induced by H2O2, up-regulate Notch expression and down-regulate P16INK4a expression. In addition, there have been some evidences indicated Kidney-nourishing could promote mesenchymal stem cells proliferate. These observations strongly suggest that, Kidney theory of TCM, as the root of the life, is associated with ESC and stem cell; it may provide insight into Kidney theory of TCM and stem cell research.

HPV DNA were detected in 60 biopsies from women with condyloma acuminata (vulva 47,Vagina 12,cervix 1)by dot blot hybridiztion;physical states of 8 HPV 11 DNA were analyzed by techniques of restriction endonuclease and Southern blot hybridization. HPV 11 was found in 19biopsies of vulva (13 ). vagina (5)and cervix (1),and HPV 16 and 18 in lvaginal and 1 vulvar biopsy respectively. All of HPV 11 DNA were episomal in cells . Our results provided the data for further studying the relationship between HPV and genital cancer in our country.

Patient engagement is considered as one pillar of new stage of health care reform and new vitality of health care quality improvement.In recent years,the rapid development of E-Health technology provides richer opportunities for patients to actively participate in health care.The article concludes the basic connotation of patient electronic engagement and proposes the framework on the basis of foreign actions as well as literature reviews,expounds the type,media and methods of patient electronic engagement as well.From the views of projects support,patient education and information rights,feasible policy measures are proposed for patient electronic engagement advancement under E-Health environment.

Objective To observe the short-term curative effect of biventricular cardiac resynchronization therapy in patients with chronic heart failure.Methods Twenty-six patients with chronic heart failure underwent biventricular cardiac resynchronization therapy,and a left ventricle lead was implanted from the coronary sinus to vein.During the 3-34(13.8?10.4)months of follow-up period,the following parameters were recorded:the cardiac function,patients' activity,heart rate at night,QRS wave width,left ventricular end-systolic dimension(LVESD),left ventricular end-diastolic dimension(LVEDD),end-systolic volume(ESV),end-diastolic volume(EDV),left ventricular ejection fraction(LVEF),left ventricular filling time,and standard deviation of time to regional peak systolic velocity(Ts-Sd-12).Results One out of the overall 26 patients died from sudden cardiac death.The cardiac function was improved in the 25 survivals in the 3-months follow-up,showing that the effective rate of CRT was 96.2%.For the 25 patients,the cardiac function was improved according to New York Heart Association(NYHA)functional class from Ⅲ-Ⅳ grade to Ⅱ-Ⅲ grade.The average total activity time was increased from 0.31?0.40 hours per day to 2.35?1.20 hours per day.Average heart rate during night-time was decreased from 84.8?15.7 beats per minute to 63.4?4.5 beats per minute,variability of cardiac rhythm was raised from 47.3?7.1ms to 96.4?15.1ms;QRS duration was shortened from 158.6?31.8ms to 129.5?30.2ms(P

Objective On the purpose of assessing inhibition efficacy and mechanism of Xiaoaiping (XAP, a kind of Chinese medicine) for SGC 7901 gastric carcinoma cellular strains.Methods 1. Extracorporal inhibition experiments were undertaken for SGC 7901 gastric carcinoma cellular strains using XAP 50,40,30,20,10mg/ml concentrations respectively. 2. SGC 7901 gastric carcinoma cellular strains on the logarithmic growth periods(4?10 7 cells) were concentrated to 1ml in volume. It was coagulated after mixing with thrombin and fibrin and then the coagulated clot was implanted in renal capsules of Kunming water mouse(certifications: No.005, CSAC) for intracorporal experiments. After continual injecting Xiaoaiping intra abdominal cavity was seven days, with 0.4,0.6,0.8ml/kg concentrations respectively. On the 8th day all the water mice were sacrificed using eyepicec ruler for calculating tumor size under microscope including inhibition hate. 3. In order to pursue mechanism of Xiaoaiping on gastric carcinoma, analyses were carried out on FACSTar with cellular analysis of cellfit DNA analysis software, after Xiaoaiping(30mg/ml) mixed with SGC 7901 cellular strains(1?10 6 cells) seven days. Simultaneously Giemsa decoration method as used for observing tumor cell morphologic changes. Results 1. Extracorporal inhibition rates were 100%,59%,29%,19% and 15% to SGC 7901 gastric carcinoma cells using XAP 50,40,30,20,10mg/ml concentrations respectively. Obvious differences were found in comparison with control groups, inhibition rate of XAP in high concentration is similar with HCPT(98%), IC 50 concentration was 21mg/ml after used XAP for seven days. 2. Tumor inhibition rates were 50%,53% and 71% respectively with continual injecting Xiaoaiping into abdominal cavity seven days by 0.4,0.6,0.8ml/kg concentrations. ED 50 was 0.44/kg for Kunming Water Mouse by logit method. Obvious differences were found in comparison with control groups. Inhibition rate(71%) of XAP in high concentration reached favorabler standard with 5 Fu (76%). 3. DNA analyses showed that cellular period were 65.70% in G 0 G 1 period, 16.22% in G 2 M period, 18.09% S period respectively. G 2/G 1 ratio was 1.86 after XAP(30mg/ml) mixed with SGC 7901 cellular strains seven days, the ratio of cellular nucleus to syrup was reduced as tumor cell morphology. Conclusion Xiaoaiping appears to have good inhibition effect on gastric carcinoma cellular strains and should be as an alternative choice for treatmenting gastric carcinoma and metastases.

Objective To design and optimize the MRS pulse sequence which used to measure gamma-aminobutyric acid(GABA) in vivo. Methods In-depth research for the MRS sequence was carried out and a theoretical analysis and simulation by software using product-operator algebra for the design and optimization of pulse sequence was discussed. Results Through the simulation of MRUI software, Cr signal at ?3.0 was seen to be suppressed and the weak GABA signal was displayed clearly. Conclusion The sequence was designed and optimized. Method to increase the efficiency and the stability of the measurement was studied. Our research gives theoretical preparation for the coming programming work.

Cyber -knife a new stereotaxic radiosurgery instrument, which structure and technical characteristics are introduced. By application of real-time image aiming and synchronous dynamic tracking techniques, abandoning vicious location frame to solve the questions of traditional directional radiosurgery technique only use pate tumour. Cyber-knife is an innovative stereotaxic radiosurgery instrument, extends the indications of stereotaxic radiosurgery from intracranial neoplasms to extracranial tumors and has a widest development prospect in medical treatment.

Objective To investigate the expression of costimulatory molecules CD80and CD86on peripheral blood lymphocytes(PBLC)in patients with systemic lupus erythematosus(SLE)and its significance.Methods The expression of CD80and CD86on PBLC was measured by flow cytometric assay in30patients with SLE and25normal controls.Results Expression of CD86on PBLC in patients with SLE was significantly lower than that of normal controls(P

Objective: To observe the effects of Tenglong Buzhong Decoction (TLBZD), a compound traditional Chinese herbal medicine, on proliferation and apoptosis of colon carcinoma cell line LS174T in vitro. Methods: Human colon carcinoma cell line LS174T and human colon epithelial cell line CRL-1790 were treated with different doses of TLBZD. Cell proliferation was detected with cell counting kit-8 (CCK-8) assay and clone formation assay. Cell cycle and apoptosis were detected by flow cytometry, and caspase-3, -8 and -9 activities in LS174T cells were detected by colorimetric assay. Results: TLBZD had no obvious cytotoxicity in normal CRL-1790 cells. After 72-hour treatment of 1 mg/mL TLBZD, or 48- and 72-hour of 2 mg/mL TLBZD, or 24-, 48- and 72-hour of 5-20 mg/mL TLBZD, proliferation of LS174T cells was significantly inhibited. Clone formation of LS174T cells was significantly inhibited by 1 to 20 mg/mL TLBZD treatment. TLBZD at doses of 5 to 20 mg/mL also induced apoptosis and cell cycle arrest at G(0)/G(1) phase in LS174T cells. In addition, caspase-3, -8 and -9 activities were significantly elevated after 5 to 20 mg/mL TLBZD treatment. Conclusion: TLBZD can inhibit cell proliferation, arrest cell cycle at G(0)/G(1) phase, and induce apoptosis in LS174T cells, which may be related to activating of caspase-3, -8 and -9.