Objective To detect the serum levels of angiogenic factors and inflammatory cytokines in patients w ith moyamoya disease and explore their roles in the pathogenesis of the disease. Methods The serum levels of vascular endothelial grow th factor (VEGF), angiopoietin -1 (Ang-1), interleukin-8 (IL-8), granulocyte colony stimulating factor (G -CSF), granulocyte-macrophage colony stimulating factor ( GM-CSF) and monocyte chemotactic protein 1 (MCP -1) in 56 patients w ith moyamoya disease and 26 healthy controls w ere measured by cytometric bead array. Results The serum levels of VEGF (2.81 ± 1.77 pg/ml vs.1.98 ±0.66 pg/ml; t = 3.081, P = 0.003 ) and IL-8 (0.89 ±0.69 pg/ml vs.0.63 ± 0.45 pg/ml; t'=2.0371, P < 0.05) in the moyamoya disease group w ere significantly higher than those in the healthy control group, and the serum level of Ang -1 in the moyamoya disease group w as significantly low er than that in the healthy control group (830.01 ±289.29 pg/ml vs.961.65 ±232.87 pg/ml; t =-2.032, P =0.045). Conclusions There are significant difference in serum levels of VEGF, Ang -1 and IL-8 betw een patients w ith moyamoya disease and healthy controls. The results indicate that angiogenic factors and inflammatory cytokines play some roles in the pathogenesis of moyamoya disease.

Objective Retinoic acid receptor(RAR?) selective antagonist,Ro41-5253(Ro) was used in the counteract test,to confirm the role of RAR? in enhancement of IgM synthesis in cord blood lymphocytes(CBLs) by retinoic acid(RA).Methods CBLs were cultured in vitro and stimulated with or without RAR? agonist RA and(or) antagonist Ro.The cells were harvested at 24 hours and 48 hours of culture time to measure the levels of gene expression of RARa,IL-4 and IL-6.ELISA was used to measure the concentration of IgM in the supernatant of 5 days culture.Results Ro could inhibit the promotion of RA on IgM synthesis in CBLs.RA could up-(regulate) RAR? gene expression,which could be restrained by Ro.Ro also could counteract the up-regulation of RA on IL-4 and IL-6 gene expression.Conclusion The effect of RA on IgM synthesis in CBLs is modulated by RAR?.

Certification ; China ; Humans ; Occupational Health Physicians

Certification ; Humans ; Occupational Health Physicians

Raman spectroscopy is an optical technology based on the theory of Raman scattering, which is generally used in the research of medical and biological science. Raman spectroscopy can be used to detect the molecular structures and components of proteins, lipids, DNA, and other biological molecules, and provide substantial information about molecules. Thus, Raman spectroscopy is generally considered a "molecular fingerprint", and it has exceptional advantages in medical research. Moreover, this technique can reflect the changes in molecular structures and detect the alterations of chemical constituents in the samples. Raman spectroscopy, given its high sensitivity and specificity in the detection of the biological samples, has been successfully used to detect and diagnose diseases in numerous sites, such as skin, oral mucosa, breast, head, and neck. In this paper, we introduce the application of Raman spectroscopy in stomatology by conducting a review of the literature.
DNA ; Lipids ; Oral Medicine ; Proteins ; Sensitivity and Specificity ; Spectrum Analysis, Raman

Objective To evaluate the damaging degree of hepatic cell in the patients with hepatocirrhosis. Methods 25 cases of hepatocirrhosis and 13 eases of normal controls were performed the dynamic imaging with 99mTc-EHIDA.By the three compartmental model configurations,the time-activity curers of the liver cell,and the function indexes liver cell extraction and excretion were calculated.Results The peak time and mean residence time in the hepatocirrhosis group were higher than those in the normal controls(P＜0.01).Compared to normal controls, the peak value,index of uptake,take slope,and excretion slope of the hepatocirrhosis were all lower(P＜0.01).Con- clusion Liver cell imaging is a better method for reflecting the change of liver cell function,and may be used to analyse the degree of hepatocirrhosis.

Objective To investigate the changes and significance of serum nitric oxide levels in the early stage after rat liver transplantation.Methods Male Sprague Dawley rats were used as do- nors and recipients of orthotopic liver transplantation,and the time of cold preservation and anhepatic phase was 4 h and 25 min respectively.Forty-eight rats were randomly divided into groups A,B and C (n=16 each).The samples of blood were taken from vena cava and hepatic tissues from left lobe 2 h, 4 h,6 h in groups A,B and C respectively after liver graft reperfusion.ALT and NO levels in serum were detected,and the expression of NF-?B in hepatic tissue was examined by immunohistochemical technique.Amounts of bile flow in 5 min after liver graft reperfused initially were measured.The pathological changes in liver tissues were observed.Results Amounts of bile flow in 5 min in group A (3.73?1.11?l) were greater than those group B (2.35?0.92?l) and group C (2.23?0.81?l) (P

Objective To investigate the effect of sodium arsenite combined with cigarette smoke solution on NF-?B in rat lymphocytes. Methods Rat lymphocytes were divided into 4 groups: the arsenite treatment group, the CSS treatment group, the arsenite and CSS treatment group, and the control group. Electrophoretic mobility shift assays was used to detect levels of NF-?B DNA binding. Western blot was used to detect protein expression of I?B?. Results Levels of NF-?B DNA binding in the CSS treatment group and the arsenite treatment group were significantly increased (P

AIM: To study the physiological function changes of the tears film after rebuilding ocular surface with corneal stem cells, and to discuss the validity and the estimate system of rebuilding ocular surface with the corneal stem cells. METHODS: The male New Zealand rabbits were used to establish the alkali burning model in the right eye. The corneal stem cells of the left eye were cultured on the amniotic membrane in vivo, and then transplanted to the right eye. Furthermore, the physiological function changes of the tear film were examined. RESULTS: Compared to the before alkali burning, the ocular surface cell morphology was similar after rebuilding ocular surface with the corneal stem cells, which were cultured on the amniotic membrane in vivo; The tear film breakup time test showed the a remarkable difference between after and before the alkali burning, but the cell modality after rebuilding had no remarkable difference compared to that before the alkali burning. CONCLUSIONS: It's an effective method to rebuild the ocular surface with the corneal stem cells in vivo, the cell framework and the physiological function of the tears film recover well after rebuilding. It may be a validity estimate system of rebuilding ocular surface to analyze framework and configuration of the ocular surface and test the tear film breakup time.

Background Pterygium is a common ocular surface disease and its treatment is surgical excision.But recurrence rate of pterygium is high after operation.Recently,some studies showed that the postoperatively topical administration of cyclosporine A (CsA) reduce the recurrence of pterygium,but this conclusion needs basis of evidence-based medicine.Objective This systematic review was to investigate the efficacy and safety of postoperatively topical usage of CsA in preventing the recurrence of primary pterygium.Methods A systematic literature retrieval was conducted and the relative articals were searched from MEDLINE,EMBASE,Cochrane Library,CNKI,Wanfang and VIP databases up to October 2012 according to searching strategy and correlated words.Randomized controlled trial (RCT) and prospective Cohort studies data were extracted by two researchers separately.The outcome parameters,such as the rate of recurrence,Schirmer Ⅰ test (S Ⅰ t) and complications were analyzed.The statistical analysis of the relative risk (RR) or weighted mean difference (WMD) was performed using a RevMan 5.0 software.Fixed-effect model was used for the inhomogeneity multiple studies (P≥0.1,homogeneity test I2 ＜ 50％),and random-effect model was used for the homogeneity study.Results Six prospective studies involving 366 eyes of 309 participants were identified,including 4 RCT and 2 non-RCT.The literature was published in 2008-2012 with the Jadad 5 scores in 1 paper and 2 scores in 3 papers for RCT and 6-7NOS (The Newcastle-Ottawa Scale) scores in 2 papers for Cohort studies.Meta-analysis showed that the recurrence and complication rates were significantly lower in the CsA group than those of the control group (RR =0.39,95 ％ CI:0.24-0.64,P =0.0002 and RR =0.24,95％ CI:0.13-0.44,P ＜ 0.00001) respectively.The postoperatively increased value of S Ⅰ t in the CsA group was significantly higher than that of the control group (WMD =2.47,95％CI:1.59-3.35,P ＜ 0.00001).Subgroups analyses suggested that the results were comparatively reliable.Conclusions Postoperative application of CsA can be effective for preventing recurrences and reducing complications after primary pterygium surgery.