1.Relationships between serum inflammatory mediators and myocardial injury in sepsis rats
Journal of Chinese Physician 2015;17(4):516-518
Objective To investigate the relationships between serum inflammatory mediators and myocardial injury in rats with sepsis.Methods Thirty male Sprague Dawley (SD) rats were randomly divided into normal control group and sepsis (0,24,48,72 h) groups.The rats subjected to sepsis were induced with cecum ligation perforation (CLP).Tumor necrosis factor alpha (TNF-α),interleukin-6 (IL-6),cardiac troponin Ⅰ (cTnI) and B-type natriuretic peptide precursor (NT-proBNP) in the venous blood were measured at 0 h,24 h,48 h,and 72 h after CLP.The lactic acid (Lac) of the arterial blood,cardiac index (CI),acute physiology and chronic health evaluation (APACHE) Ⅱ score were also determined simultaneously.Results The levels of TNF-α,IL-6,cTnI,NT-proBNP,and Lac in sepsis (24 h,48 h,and 72 h) groups were significantly elevated compared to normal control and sepsis (0 h) group.In addition,the number of sepsis (24 h,48 h,and 72 h) groups progressively increased (P <0.05).Conclusions It existed a close relationship between serum inflammatory mediators and myocardial injury,and made myocardium damage after CLP.
2.Evaluation of degree of hepatocirrhosis with liver cell magong by SPECT
Xiao-Bing LIU ; Yi-Xiang FANG ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(08):-
Objective To evaluate the damaging degree of hepatic cell in the patients with hepatocirrhosis. Methods 25 cases of hepatocirrhosis and 13 eases of normal controls were performed the dynamic imaging with 99mTc-EHIDA.By the three compartmental model configurations,the time-activity curers of the liver cell,and the function indexes liver cell extraction and excretion were calculated.Results The peak time and mean residence time in the hepatocirrhosis group were higher than those in the normal controls(P<0.01).Compared to normal controls, the peak value,index of uptake,take slope,and excretion slope of the hepatocirrhosis were all lower(P<0.01).Con- clusion Liver cell imaging is a better method for reflecting the change of liver cell function,and may be used to analyse the degree of hepatocirrhosis.
3.Clinical characteristics and prognostic factors of 236 patients with diffuse large B cell lymphoma.
Liang-Liang MA ; Jin YUAN ; Bing XIANG
Chinese Journal of Hematology 2012;33(9):768-770
Adolescent
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Adult
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Aged
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Aged, 80 and over
;
Female
;
Humans
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Lymphoma, Large B-Cell, Diffuse
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diagnosis
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Male
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Middle Aged
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Young Adult
5.Efficacy of zishen prescription on the learning and memory-abilities of aging model rats.
Lai-xiang LIN ; Yuan ZHANG ; Bing SUN
Chinese Journal of Applied Physiology 2004;20(3):289-290
Aging
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psychology
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Animals
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Drugs, Chinese Herbal
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pharmacology
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Learning
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drug effects
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Male
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Memory
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drug effects
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Rats
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Rats, Wistar
7.THE TWO ISOENZYMES OF S.CEREVISIAE GLYCEROL 3-PHOSPHATE DEHYDROGENASE
Gui-Xiang LIU ; Bing-Qi YU ; Bin ZHUGE ; Jian ZHUGE ;
Microbiology 1992;0(05):-
NAD +-dependent cytosolic glycerol-3-phosphate dehydrogenase in Sacch aromyces cerevisiae is one of the key enzymes in metabolic pathway of glycerol . catalysing the reduction of dihydroxyacetone phosphate to glycerol-3-phosph ate.It has two isoenzymes.To study the differences between their structures, their expression of encoding genes and their functions may help increase the understan ding of the cell response mechanism to the hyperosmotic and anoxic conditions. In this paper the research on the two isoenzymes was reviewed.
8.Prokaryotic expression and purification of human Smith D1 antigen
Wen-Bing WU ; Xiao-Peng LAN ; Xiang-Yue YANG ;
Chinese Journal of Laboratory Medicine 2000;0(06):-
Objective To obtain recombinant human Smith D1 (Sm D1) antigen and establish detecting assay.Methods Human Smith D1 antigen was synthesized by PCR using human Leukemic cDNA. The prokaryotic expression vector pGEX-ST-Sm D1 was constructed and transformed into E.coli.BL21 cell.Protein expressed under the induction of IPTG.We established DIGFA for detecting anti-Sm D1 antibodies with purified Sm D1 antigens.Results Sequence and restriction analysis revealed Sm D1 gene was cloned in frame into pGEX-5T,SDS-PAGE profile showed a clear protein band with a relative molecular weight of 39 000 and western blotting indicated that the expressed product specifically reacted to polyclonal anti-human Sm D1 genes.There was no significant difference between DIGFA and IB.The agreement between DIGFA and IB was 91.7% as calculated by Kappa statistical method.The sensitivity and specificity of DIGFA were 100% and 83.3% repectively.Conclusions Human Sm D1 gene is successfully cloned、 expressed and purification.The DIGFA,using purified Sm D1 antigens,is as good as IB,rather simpler, more rapid and reliable assay.
9.Enforce the Student's Base,Cultivate Their Abilities
Xiang-Mei ZHANG ; Tong-Suo MA ; Kun LIU ; HONG-BING ;
Microbiology 1992;0(06):-
It is important to pay more attention to students’ basic skills training and their comprehensive abilities cultivating in microbiology experiment teaching. Explorations and reforms in enforcing the students’ base and cultivating their abilities were carried out in order to improve teaching quality and train specialized talents with high quality.
10.English Teaching Experience of Microbiology Course Using Original Edition of English Textbook
Xiao-Feng TANG ; Bing TANG ; Xiang-Dong CHEN ; Ping SHEN ;
Microbiology 2008;0(09):-
The practice of English teaching of microbiology course using the original edition of Microbiology (Prescott LM et al., 5e, 2002) as textbook have been carried out for two years for undergraduate students majoring in Biology. The strengths of this book are comprehensive coverage, flexibility of organizing, and emphasis on the enhancing the overall ability of students. Its content is excellent with fundamental knowledge, systematic arrangement and up-to-date currency, which helps instructor to construct a suitable system in the course structure and curriculum, as well as to enrich the teaching content and to increase teaching effectiveness. The selection of original edition of English textbook in microbiology course is useful to train high-quality biological talents possessing international view and the adaptive ability of globalization process.