Objective To investigate the relationship between Chlamydia pneumoniae(CP),Helicobacter pylori(HP),high-sensitivity C-reactive protein(hs-CRP) and the stability of carotid artery atherosclerotic plaque(CAP).Methods One hundred and twenty patients with carotid artery scleratheroma,assigned as CAP group,were divided into stable plaque group(n=37) and unstable plaque group(n=83) based on imaging and pathological examinations.Eighty-six elderly individuals,who underwent health examination in the same period,were assigned as control group.The serum levels of CP IgG,and HP IgG of all the involved individuals were determined by enzyme linked immunosorbent assay(ELISA),and serum level of hs-CRP was measured by immunoturbidimetry.Results There existed significant differences in positive rate of CP IgG,HP IgG and CRP level between CAP group and control group(P

Objective:To investigate the effect of high-volume hemofiltration(HVHF) on serum levels of IL-6 and pulmonary expression of IL-6 mRNA in pigs with multiple organ dysfunction syndrome(MODS).Methods: Twenty-five pigs were randomly divided into model group,control group and HVHF group.Animals in control group were injected with normal saline,and those in the other 2 groups were treated with "a second hit" to establish MODS model,then animals in HVHF group was treated with HVHF for 3 days.Serum IL-6 levels were determined by ELISA and Real-time quantitative PCR was used to determine the expression level of IL-6mRNA.Lymphocyte apoptosis was detected by Annexin-Ⅴ/PI flow cytometry.Results: After HVHF,the serum indices associated with organ-function and the morbidity and mortality were much lower than those in MODS model group(P

The article reorganizes the complaint data from 30 cases of clinic patients,makes analysis on the risky factors causing the complaint and the problems existent in the clinic management,as well as puts forwards concerned effective preventive measures.

A efficient and convenient method for covalent binding of antibodies to liposomes was de-scribed.Antibodies were attached to the aldehye groups on the gangliosides by the periodate ox-idation to form schiff—base and the complex was stabilized by reduction.Separate the liposome —IgG complex from unbound IgG using discontinous dextran T40 gradient centrifugation.AKP and HRP were coupled to liposomes,and both enzymatic activity was remained more than 80%.and compared this with glutaraledhyed method.

Objective To explore the expression of PIN1 and Cyclin E in gastric carcinoma tissue after radical gastrectomy and its significance.Methods 46 patients received radical gastrectomy were selected as study subjects.Cancer tissues were harvested as the gastric cancer group,and the adjacent normal tissues of tumor tissue were harvested as the control group.The expression of PIN1 and Cyclin E was observed by immunohistochemistry,and the relationship with tumor infiltration,differentiation,lymphatic and distant metastasis were analyzed.A five year followup study was also applied.Results The expression of PIN1 (3 949.66 ± 157.40) and Cyclin E (5 443.07 ±240.15) was significantly higher than those of the control group[(247.67 ± 75.63),(354.92 ± 93.88)] (all P ＜0.05),and the expression of PIN1 was related with tumor differentiation,lymphatic and distant metastasis,and the expression of Cyclin E was closely related with tumor infiltration and differentiation.The 5-year survival rate of patients with the simultaneously increased expression of PIN1 and Cyclin E was 50.0％,which was significantly lower than the expression of the two separate elevated or not elevated.Conclusion The expression of PIN1 and Cyclin E in cancer tissues after radical gastrectomy is high,and could better reflect the pathological parameters and prognosis.

Objective To investigate the prognostic factors of curative operation for spontaneous rupture with hemorrhage of primary hepatic carcinoma (SRHC).Methods The clinical data and followed up results of 41 patients with SRHC treated by curative operation,were retrospectively analyzed.The prognostic factors affecting survival were studied by univariate and multivariate analysis.Results The survival rate of 1-year,3-year,5-year was respectively 46.34％,21.95％,9.76％.Univariate analysis showed that Child-Pugh class,degree of accompanying cirrhosis,tumor size,operation time,blood loss,tumor staging,serum albumin were significant prognosis factors.The use of Coxs multivariate proportional hazard model indicated that significant prognosis factors by curative operation for SRHC were degree of accompanying cirrhosis,Child-Pugh class,blood loss,tumor staging for cumulative survival rate.Conclusion The factors affecting prognosis were degree of accompanying cirrhosis,Child-Pugh class,blood loss,tumor staging.

AIM: To evaluate the effect of EffecteneTM lipofectine mediated plasmids encoding human pcDNA4-vascular endothelia growth inhibitor (pcDNA4-VEGI) gene on corneal neovascularization (CNV).METHODS: Forty New Zealand albino rabbits were sutured by 5- 0 silk on the superior cornea to induce CNV and divided into 4 random teams, ten per each team: team A: transfected by pcDNA4-VEGI gene mediated by EffecteneTM lipofectine transfection; team B: by plasmid pcDNA4; team C: by EffecteneTM, and team D: by normal saline. Length and area of CNV were observed under slit lamp every day after transfection. Immunohistochemistry was performed to detect the expression of VEGI protein in corneas at day 3, 7, 14 and 21. RESULTS: 1) Average occurrence of CNV was 6.3 days in team A, 3.1 days in team B, 3.2 days in team C, and 3.2 days in team D. Difference was significant between A and other teams (P<0.01); 2) Length and average area of CNV in each period in team A was significantly different from those in team B, C and D (P<0.01); 3) VEGI expressions were observed in epithelium, stroma, endothelium and the cliff of CNV in team A at 3 days after transfection by immunohistochemical staining. None VEGI positive cells were found in the control teams (team B, C and D) all the time.CONCLUSION: EffecteneTM lipofectine transfection technique can effectively transfect pcDNA4-VEGI gene into rabbit cornea and the length and CNV areas can be inhibited by VEGI gene.

Adolescent ; Adult ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Cytarabine ; administration & dosage ; therapeutic use ; Female ; Humans ; Leukemia, Myeloid, Acute ; drug therapy ; Male ; Middle Aged ; Prognosis ; Treatment Outcome ; Young Adult

Melatonin is a bioactive substance primarily secreted by the pineal gland.Increasing evidences indicate that melatonin is effective in reducing breast cancer development.Melatonin exerts its anticarcinogenic actions through a variety of mechanisms.Melatonin suppresses estrogen receptor gene,modulates several estrogen dependent signal transductions,inhibits cell proliferation and impairs the metastatic capacity and so on.It has been suggested that enhanced endogenous melatonin secretion or melatonin treatment is beneficial for breast cancer patients.This review describes the mechanisms of melatonin on breast cancer and its possible application.

Objective To design an intravenous indwelling needle in battle field aid.Methods Several concavity were made on the hollow tube inner surface posterior puncture needle case,and some corresponding convexity uplifted peripheral needle handle pistons posterior puncture needle core.Revolution could be avoided because the needle handle pistons were just embedded in hollow tube concavity.It was convenient to operate and decreased the operator's orientation transformation by the needle's handhold vane adopting rectangle instead of butterfly shape.Results The improved puncture needle was stable and the rate of success of intravenous puncture was increased.Conclusion The improved venous retaining needle can be applied in battle field aid.