The level of CD4~+ CD25~+ regulatory T cells was assayed in the peripheral blood from the patients with Graves' disease (GD) by floweytometry.CD4~+ CD25~+ regulatory T cells as a kind of non-specific immune cells appear to have no relationship with thyroid function,however,their lowered number in early GD patients suggests that they seem to play an essential role in the pathogenesis of GD.

AlM: To discuss the efficacy of modified dacrocystorhinostomy with intubation in the treatment of chronic dacryocystitis combined with lacrimal duct narrow or obstruction.
METHODS: Retrospective analysis of 56 patients who underwent modified dacrocystorhinostomy with intubation in our hospital from July 2010 to September 2011.
RESULTS: All patients underwent the extubation of lacrimal and catheters irrigation, the patency rate was 95% at 2a after operation.
CONCLUSlON: Modified dacryocystorhinostomy combined with intubation is a effective method for chronic dacwocystitis combined with lacrimal duct obstruction, which has simple operative procedure, high success rate.

Academies and Institutes ; Carcinoma in Situ ; diagnosis ; immunology ; Evaluation Studies as Topic ; Humans ; Immunohistochemistry ; methods ; standards ; Internationality

Cholestasis ; etiology ; physiopathology ; therapy ; Female ; Humans ; Liver Diseases ; drug therapy ; etiology ; physiopathology ; Male ; Neonatology ; Parenteral Nutrition, Total ; adverse effects ; S-Adenosylmethionine ; therapeutic use ; Ursodeoxycholic Acid ; therapeutic use

Lentiviral vectors can transduce a wide range of different cell types.It can transduce not only dividing cells but also nondividing cells.It can be the immunotherapy tool for cancer therapy.Lentiviral yeators have several advantages,amongst which long-term expression,low toxicity,high capacity and low antivector immunity,they will play an important role and will attract more and more interest as an immunotherapeutic.Here,we discussed the clinical application and safety of the lentiviruses encoding tumor-associated antigen(TAA).

OBJECTIVE To investigate the classification and antibiotics resistance of Staphylococcus in children with septicemia in recent two years and to provide data for clinical treatment. METHODS All the blood specimens delivered by pediatrics were identified and detected the antibiotic resistance with Microscan Auto SCAN4. RESULTS Totally 268 Staphylococcus strains were collected. Among them,S. aureus was 114 strains (42.5%),coagulase-negative Staphylococcus were 154 srains. Among 114 S. aureus strains,meticillin-resistant Staphylococcus aureus (MRSA) were 79 strains (69.3%). Among 154 coagulase-negative Staphylococcus strains,meticillin-resistant coagulase-negative Staphylococcus aureus (MRCNS) were 102 strains (66.2%). The drug sensitivity results showed that meticillin-resistant staphylococcus (MRS) were highly resistant to 15 commnly used antibiotics than meticillin-sensitive Staphylococcus (MSS) and showing multi-drug resistance. No vancomycin-resistant strains were found.CONCLUSIONS Children with septicemia infected by Staphylococcus are increasing recently. Identification and antibiotic resistance of clinical isolated Staphylococcus must be strengthened,and give the effective data for clinicians.

Objective:To study the influence of GPC3 gene on the proliferation,adhesion and invasion of hepatoma cell line SK-Hep-1.Methods:SK-Hep-1 cells were transfected with pEGFP-N2-GPC3 using Lipofectamine2000.RT-PCR was used to examine the GPC3mRNA expression in GPC3-SK-Hep-1 cells.MTT assay was used to examine the proliferation and calculate the adhesion rate of SK-Hep-1 cells.Transwell system was used to assess the migration and invasion of the cells.Results:SK-Hep-1 cells were successfully transfected with pEGFP-N2-GPC palsmid.GPC3 mRNA was detected in SK-Hep-1 cells.Transfection with CPC3 significantly suppressed the growth of SK-Hep-1 cells(P

Air Pollutants, Occupational ; analysis ; Chromatography, Gas ; methods ; Dimethylamines ; analysis ; Methylamines ; analysis ; Workplace

BACKGROUND: The biliary stents commonly used in clinic are made of plastics and metal, both of which have some irresolvable defects. New type of material characterizing by great biocompatibility and degradation for the making of biliary stents needs to be found and studied.OBJECTIVE: To evaluate the biodegradation law and the change in radical force of five different mole-ratios of biliary stents made of poly (lactic-co-glycolic acid) (PLGA) in human bile in vitro.DESIGN, TIME AND SETTING: A Completely random design was performed at the Laboratory of Hepatobiliary Surgery, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University between July and December 2006.MATERIALS: Five different mole-ratios of PLGA hiliary stents (90L/10G, 80L/20G, 70L/30G, 60L/40G, and 50L/50G) were provided by Dalgang Biological Science and Technology Co., Ltd., Jinan; Human fresh bile was obtained from the patients who underwent external drainage of bile in the Department of Hepatobiliary Surgery, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University.METHODS: Thirty-two PLGA biliary stents of each proportion were respectively divided into eight groups, with four stents in each group. All of them were independently submerged into test tubes with 10 mL fresh human bile which were then placed in constant temperature oscillator. The temperature was set at 37 ℃. The bile was changed every day. After incubated for one week to eight weeks, the PLGA biliary stents were respectively taken out of test tubes from the first to the eighth group each week. They were washed with distilled water and dried in calorstat for 24 hours.MAIN OUTCOME MEASURES: ① Gross morphology, color and integrality of PLGA stents; ② Scanning electron microscope; ③ the mass and the radical force of PLGA stents.RESULTS: ① Five different mole-ratios of PLGA stents followed a similar changing law as to gross morphology: stents remained intact → stents became soft → the surface ofstents became coarse and cracked → stents collapsed → stents were degraded into pieces → stents were completely degraded. ② Before degradation: limpid stereo reticular structure of PLGA; One week after degradation: PLGA was corroded and the reticular structure was decreased; Three weeks after degradation: airspace was gradually disappeared and the reticular structure was further decreased; Five weeks after degradation: airspace and the reticular structure were completely damaged, which was in an indefinite form. ③ The mass loss of each proportion of PLGA stents gradually speeded up along with the time extension. Furthermore, with the content of PGA increasing, the mass loss of the stents became faster. The initial radical force was different among five different mole-ratios of hiliary stents. With the content of PLA increasing, the radical force of the stents became stronger.CONCLUSION: The material of PLGA possesses adjustable biodegradation and good mechanical feature, so it is suitable for preparation of biliary stents.

Objective To evaluate the application of totally implantable central venous access ports. Methods A retrospective a-nalysis of 217 cases was made on the application and complication of totally implantable central venous access ports from October 2003 to May 2008. Results Venous access ports, which were connected to central venous system via subclavian vein in 199 cases and jugularis interna vein in 17 cases, were implanted into subcutaneous of chest wall. The success rate of implantation was 99.6% (216/217). The ports worked well in 207 cases (95.8%), and 9 ports (4.2%) were removed due to complications. Associated complications included subclavi-ana arterial puncture in 3 cases (1.4%) and local hematoma in one patient. Ventricular tachycardia occurred in one patient due to catheter was inserted into the right ventricle (0.4%). Five patients had soft tissue infections around ports (2.3%) and the ports were removed in 2 eases due to anti-infections therapy failure. Catheter dislocation happened in 2 cases (0.9%). The catheter obstruction was observed as a complication, which comprised three instances of catheter distortion and three of catheter-associated venous thrombosis, the ports in these six patients were removed (2.8%). The overall complication was 7.8%. Conclusions Totally implantable central venous access ports can provide a long-term and safe intravenous access to patients who need long-term intravenous infusion, especially for cancer patients received chemotherapy. Although the operation is simple, there are some complications and need to be carefully monitored.