Objective:Study of aprotinin effect on patients immunity system and complement during open heart operation.Methods:Ig and complement in patients' blood were measured respectively before operation and on the operative day and the first,second,third postoperative day.Results:In both aprotinin and comparative groups,the plasma levels of IgG,IgA,IgM,IgE and complement C3,C4,CH50 dropped remarkably and began to rise from the first postoperative day.On the third postoperative day, the plasma levels of IgG,IgA and complement C3,CH50 basically restored to the levels before operation.While IgM,IgE and complement C4 in aprotinin group and all Ig with complement in comparative group did not.Conclusion:Aprotinin has not a protective function to patients' immnunity system and complement during open heart operation but it can improve their restoration postoperatively.

Objective To discuss the pattern and effect of reading report activities on college-to-undergraduate nursing students in adult higher education. Methods We chose 154 college-to-undergraduate nursing students in adult higher education who studied at our department from 2009 to 2012 as control group, meanwhile the experimental group consisted of 168 college-to-undergrad-uate nursing students in adult higher education who studied at our department from 2013 to 2014.We used thefive-in-oneteaching method involving relevant scientific knowledge training, tutorial system, group report meeting, exchange of excellent reading report and score checking to train the experimental group, while the control group was still trained by primary method which was relevant scientific knowledge teaching and discussion of excellent reading report. Results The reading reports wrote by the students in the experimental group were superior to those wrote by the control group (P<0.001);The experimental group had better scores in the achievements of scientific knowledge and theory (P<0.001); The self-assessment of experimental group students in the ability of literature review, reading and analysis was higher than that of the control group (P<0.001).As to the effects of reading report such as enhancing scien-tific awareness, expanding scope of knowledge and so on, the experimental group had a obvious advantage over the control group ( P<0.001). Conclusion The five-in-onetraining pattern in the reading report activities involving relevant scientific knowledge training, tutorial system, group report meeting, exchange of excellent reading report and score checking can effectively improve the scientif-ic awareness of college-to-undergraduate nursing students in adult higher education, which is conductive to establish the basis of nurs-ing scientific research, guide the clinical nursing work and improve the comprehensive quality of students.

Objective To study the change of the structural and biological properties of the cardiovascular tissue after cell extracting. Methods The native and treated pulmonary cusp and pulmonary artery wall were evaluated by HE stain, ETVG stain, immunochemistry stain and scan electron microscopy. The contents of the main macro-molecules were measured. The content and position of calcium was investigated by subcutaneous implantation in a rat model. Results The acellularization procedure resulted in an almost complete removal of the original cells while the construction of the matrix remained. The contents of colleagen and elastin increased while soluble GAG reduced. The content of calcium in acellular tissue by xenogenic implantation was less obviously than that in fresh one. Conclusion A suitable acellularization procedure can remove all cells and most soluble proteins, and matrix remains nearly intact, so as to alleviate obviously calcification via xenogenic implantation in vivo.

The lengths and depths of occlusal rest base slope were calcukted accurately when the inclinations of occlusal rest base were 10?,15?and 20?at 1/2, 1/3 and 1/4 crown width respectively. The data will be usefull in the process of removable denture design with computer and provide a suitable way for the preparation of tilt abutment.

Resident or specialist training plays a very important role in developing qualified clinicians and improving medical quality.In combination with the status quo of resident or specialist training in our country , brief analysis has been made on the characteristicsof military resident or specialist training .As a result, the 3 +X +militarymode has been put forward in military resident orspecialist training.According to Specialist Training Standards by National Health and Family Planning Commission , training criteria upto military requirements have been established in order to achieve systematic military residency , develop medical support capability andpromote military health development.

Objective To explore the clinical efficacy of Wenshen Juanbi Deco ction combined with warm acupuncture in the treatment of cold and dampness lumbar disc herniation. Methods Retrospective analysis of our hospital in April 2015 to February 2017 treatment of 60 cases of cold and humid lumbar disc herniation in patients with clinical data, according to different treatment methods were divided into control group and the study group,with 30 cases in each group.The control group underwent routine single Pelvic traction treatment, the study group underwent Wenshen Juanbi decoction combined with warm acupuncture treatment, The symptom improvement after treatment in two groups was compared. Results The total effective rate was 86.67% (26/30) in the study group and 58.06% (18/30) in the control group. The difference between the two groups was statistically significant (P<0.05). Compared with before treatment, the index of lower and lower extremity radiating pain, straight leg raising test, lower limb skin sensation, tendon reflex, spinous process tenderness and limited waist activity after treatment in the two groups were significantly improved (P<0.05). And the improvement effect of the study group was better than that of the control group (P<0.05). Conclusion Wenshen Juanbi Decoction combined with warm acupuncture is effective in the treatment of cold and dampness lumbar disc herniation, which can effectively improve the clinical curative effect and improve the clinical symptoms of patients with high clinical application and popularization value.

Epilepsy ; chemically induced ; Humans ; Poisoning ; physiopathology ; Xylenes ; poisoning

Objective To investigate the effect on the differentiation of bone marrow mesenchymal stem cells (BMSC) with non-contact co-culture with mechanical stimulated ligament fibroblasts. Methods A cyclic 10% uniaxia strain at 1 Hz was applied on rat pelvic ligament fibroblasts, then were co-cultured with BMSC for 3, 6 and 12 days in non-contact condition. The protein expression of collagen Ⅰ ,Ⅲ in BMSC were detected by SP method and revealed by the mean gray value. The mRNA expressions of collagens type Ⅰ and type Ⅲ in the BMSCs were measured with real-time (RT)-PCR ,and the results were indicated by the ratio between the mRNA and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) . Results (1) Protein expression; after 3 days co-culture with pelvic ligament fibroblasts, expression of collagen Ⅰ and Ⅲ in BMSC are 82. 4 ± 3. 4 and 76. 8 ± 2. 5. When compared with 80. 2 ± 2. 6 and 74. 6 ± 1. 1 in BMSC without co-culture, there was no significant difference (P > 0. 05) . After 6 days co-culture with pelvic ligament fibroblasts, the expression of collagen Ⅰ and Ⅲ of 126. 6 ±2. 2 and 118. 6 ± 1. 4 in BMSC were significantly higher than 82. 7 ±3. 0 and 76. 2 ± 1. 3 in BMSC without co-culture (P < 0. 05). Similarly, after 12 days co-culture with pelvic ligament fibroblasts, the expression of collagen Ⅰ and Ⅲ of 135. 3 ±3. 4 and 128. 7 ± 2. 6 in BMSC were significantly higher than 86. 6 ± 1. 3 and 81. 8 ± 1.4 in BMSC without co-culture (P <0.05). (2)mRNA expression:after 3 days co-culture with pelvic ligament fibroblasts , the mRNA expression of type Ⅰ and type Ⅲ collagens in BMSC are 2. 10 ±0. 20 and 1. 20 ±0. 30. When compared with mRNA expression of 2. 01 ±0. 12 and 1. 13 ±0.21 in BMSC without co-culture, no significant difference were observed (P > 0. 05). After 6 days co-culture with pelvic ligament fibroblasts , the mRNA expressions of type Ⅰ and type Ⅲ collagens mRNA were 5. 60 ±0. 21 and 2. 61 ±0. 20, which were significantly higher than 3. 70 ±0. 33 and 1. 82 ± 0. 14 in BMSC without coculture (P < 0. 05). After 12 days co-culture with pelvic ligament fibroblasts, the mRNA expressions of type Ⅰ and type Ⅲ collagens of 5. 91 ±0.31 and 2. 92 ±0. 23 were significantly higher than 4. 04 ±0. 21 and 2. 04 ±0. 13 in BMSC without co-culture (P <0. 05). Conclusion Non-contact co-culture with mechanical stretch stimulated ligament fibroblasts, it might promote synthesis of types Ⅰ and Ⅲ collagen in rat BMSCs and induced BMSC differentiated into pelvic ligament fibroblasts.

Objective To better promote the health education and health promotion among patients with chronic disease, general practitioners (GPs) in the community should master the knowledge of non-communicable diseases (NCDs). This paper aimed to describe the current situation of GPs' knowledge about prevention and treatment of NCDs. Methods Self-designed questionnaires were issued to 1 077 GPs in community health service centers, and all GPs finished the questionnaire without assistance. The content of questionnaire includes the general information of GPs and six questions focusing on NCDs. Results Overall, 1 076 GPs completed the questionnaire, and the response rate was 99.9% (1 076/1 077). With regard to the six questions about NCDs, 90.4% (973/1 076) of GPs were well aware of the diagnosis of hypertension. Apart from that, the knowledge of diabetes mellitus (60.9%, 655/1 076), factors related to NCDs (70.2%, 755/1 076), the concepts of mental illness (52.0%, 560/1 076) and the clinical significance of tumor markers (87.9%, 946/1 076) among GPs were adequate, while more than half of the GPs did not know a lot about home oxygen therapy. Conclusion The mastering level of knowledge about prevention and treatment of NCDs among GPs varies that they need further improvement.

According to the correlative analyses on Chinese medicine essence, dosage forms and quality control level, it expounds the precise concept of Chinese medicine, and its quality advantages and characteristics in this paper, furthermore discusses how to achieve the ideal drugs and Chinese medicine quality precision in expectation. Base on the Chinese medicine essence, using the concept of nature medicine and its drug system to construct Chinese medicine effective material basis and its drugs, with the correlative analyses of whole view and reductionism, the problems of uncertainty quality of original natural medicinal resources and preparations may well be solved, and further with the macroscopic to microcosmic construction of drug system, the precision in expectations of Chinese medicine quality and higher production lever may well be achieved.
Drugs, Chinese Herbal ; chemistry ; pharmacology ; standards ; Humans ; Medicine, Chinese Traditional ; Plants, Medicinal ; chemistry ; Precision Medicine ; Quality Control