3.Study on the Quality Standard of Root of Urtica dioica and Different Harvest Periods
Hong QIU ; Bing DAI ; Yongxue PAN
China Pharmacy 2015;(24):3431-3433
OBJECTIVE:To study the quality standard of root of Urtica dioica and investigate the sample’s content in different harvest periods. METHODS:TLC was conducted to identify the scopoletin in the root of U. dioica with the developing agent of hexane-dichloromethane- ethyl acetate- formic acid (6∶10∶7∶1.2,V/V/V/V);alcohol-hot dipping was used to determine the con-tent;and the root of U. dioica in different harvest periods were comparatively researched. RESULTS:The lactone thin-layer chro-matogram feature was obvious,alcohol-hot dipping showed the contents of root of u. dioica was relatively high(RSD≤1%),the ex-tract content of root of U. dioica was the highest from late fall to early spring,and the stage was suitable excavation period. CON-CLUSIONS:The established quality standard can be used for accurately qualitative and quantitative,and studying different harvest periods is conducive to effectively control the quality of root of U. dioica.
4.The effects of different centrifugal conditions on plasma B-type natriuretic peptide sample testing
Renjiang TANG ; Bing QIU ; Chunshu ZHENG
International Journal of Laboratory Medicine 2015;(4):516-517
Objective To investigate the plasma separation after samples to laboratory under different centrifugal conditions of b-type natriuretic peptide (BNP)the influence of the test results.Methods Randomly selected 20 cases of hospitalized patients at our hospital,the vacuum EDTA-K2 anticoagulant blood,respectively from several different centrifugal speed and time after centrif-ugal separation of plasma,the Siemens ADVIA Centaur CP on automatic chemiluminescence meter yet.Results Based on the same patients at the same time of blood specimens,under different centrifugal conditions and preparation of plasma BNP results meas-ured by comparative analysis,the centrifugal rotational speed of 3 000 r/min,the centrifugal time reach more than 5 min for the plasma specimens centrifugal conditions required by the test results and the kit standard preparation of plasma determination results difference has no statistical significance (P >0.05).Conclusion As soon as possible in order to determine the BNP as a result,es-pecially emergency specimens at night,when the centrifugal rotational speed and time to meet the 3 000 r/min and 5 min,can ob-tain accurate detection results,for clinical results as soon as possible for the BNP,diagnosis heart failure to win the time.
5.Detection of antibodies to Epstein-Barr virus with ELISA based on fusion capsid protein
Qingfang QIU ; Yun WANG ; Bing LUO
Chinese Journal of Laboratory Medicine 2009;32(9):1002-1005
and 98.6% (436/442), respectively. Conclusion The ELISA based on fusion viral capsid proteins is sensitive, specific and accurate method for determining antibodies to VCA of EBV for both clinical diagnosis and epidemiology studies.
9.Effects of physiological testosterone on transcription factor activity in human umbilical vein endothelial cells.
Hong JIN ; Wen-Bing QIU ; Geng PENG
Chinese Journal of Applied Physiology 2008;24(3):347-376
Cells, Cultured
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Flutamide
;
pharmacology
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Human Umbilical Vein Endothelial Cells
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cytology
;
drug effects
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metabolism
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Humans
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Proto-Oncogene Proteins c-myb
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metabolism
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Sp1 Transcription Factor
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metabolism
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Testosterone
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antagonists & inhibitors
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physiology
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Transcription Factors
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metabolism
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Tumor Suppressor Protein p53
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metabolism
10.Data base establishment and data mining on regular pattern of TCM beauty prescription
Jiarui WU ; Ling DONG ; Bing ZHANG ; Pu WANG ; Wenhuan QIU
International Journal of Traditional Chinese Medicine 2012;34(9):815-817
ObjectiveTo explore the regular pattern of TCM beauty prescription.MethodsBased on the ACCESS database platform,we established TCM beauty prescription database and analyzed the information in detailed.ResultsThe total number of beauty prescription in the database was 1821.We focused on analyzing the frequency of medicine application in ancient beauty prescriptions,medicinal diet,acne,chloasma and freckles prescriptions.Statistics showed that dahurian angelica root,indian bread,medlar,honey,black sesame,baical skullcap root,and Chinese angelica were the most commonly used seven kinds of Chinese herbs.ConclusionThe main parts of Chinese medicines for making beauty were those herbs which can nourish yin and blood,supplement yang and qi; and the main parts of Chinese medicine for treating skin diseases were those herbs which can clear heat and detoxicate.