Objective To observe and study the clinical efficacy of laparoscopy combined with hysteroscopy in treatment of obstruction of fallopian tube.Methods 72 cases of patients who were treated for fallopian tubal obstruction were selected as the research object,and they were randomly by simple randomization divided into two groups,the observation group and the control group,with 36 cases in each group.The hysteroscopic treatment was used in the control group,hysteroscopy combined with laparoscopy were treated in the observation group.Then operation effect and 1 years after surgery in pregnancy of the two groups were compared.Results In the control group,70 fallo-pian tube had 56 successful recanalization with the recanalization rate of 80%.At 3 months after operation it blocked 8,restenosis rate was 14.2%.In the observation group,68 fallopian tube had 62 successful recanalization with the recanalization rate of 91.1%,and at 3 months after operation it blocked 10,restenosis rate 16.2%.The recanalization rate in the observation group was significantly higher than that of the hysteroscopy wire group(χ2 =6.231,P <0.05). The restenosis rate was no significant difference between the two groups(P >0.05).The control group of 14 cases with intrauterine pregnancy,the pregnancy rate was 38.9%,1 cases of tubal pregnancy,the pregnancy rate was 2.7%.24 cases of pregnancy with intrauterine in the observation group,the pregnancy rate was 66.7%,2 cases of tubal pregnancy,the pregnancy rate was 5.4%.The intrauterine pregnancy rate in the observation group was signifi-cantly higher than that of the hysteroscopy group,there was significant difference(χ2 =5.568,P <0.05),fallopian tube pregnancy rate was no significant difference between the two groups(P >0.05 ).Conclusion Hysteroscopy combined with laparoscopy in treatment of fallopian tubal obstruction can make up for the deficiency of hysteroscopic treatment alone,it can greatly improve the fallopian tube recanalization rate and pregnancy rate after operation,it is worthy for clinical application.

Adenoviridae ; genetics ; Cell Line ; Genetic Vectors ; Humans ; Nitric Oxide Synthase Type III ; genetics ; Virus Replication

Objective To learn and analyze the patients' circulation of blood fluctuating rules in 24 hours after receiving the liver transplantation, conclude the policies of the circulation of blood monitor and nursing, and offer the basement for the clinical nursing program of liver transplanting. Methods Using the multifunctional electrocardiograph to observe the hver-transplanted ICU patients' heart rate , blood pressure and center venous pressure in the time points below: before operation, turning back ICU immediately and 2、6、12、 24、20、24 hours, and make the statistical analysis. Results The patients' heart rate ascend greatly after entering ICU 24 hours( P < 0. 01 ). When turning back ICU, their blood pressure had no greatly different (P > 0. 05). After 2 hours,the blood pressure become to distinct greatly until 24 hours. Center venous pressure had no statistical difference( P >0.05). Conclusions The liver-transplanted patients' circulation of blood changes greatly after operation. To avoid damage, we should strengthen the temperature monitor, and standardize the patient nursing management.

OBJECTIVEThe relationship between latent adenovirus infection and airway inflammation have not been well documented. The aim of this study is to illustrate the roles of adenovirus E1A protein on the level of glutathione (GSH) in response to oxidative stress and the effect of the oxidant/antioxidant imbalance upon the transactivation of NF-kappaB triggered by E1A protein.

METHODSRat alveolar epithelial cell stably expressing adenoviral E1A or control plasmid were developed. For isolation of nuclear extracts, 5 x 10(5) cells were plated and grown overnight in 60 mm dishes. Experiments were repeated three times. The cell model of stably expressing adenoviral E1A was stimulated by H2O2. The level of GSH were measured. E1A positive clone was stimulated by LPS or TNF-alpha and treated with L-Buthionine-sulfoximine (BSO). The expression of NF-kappaB was measured by Western blot. Differences between groups were assessed for significance by Student' t test; multiple comparisons by the one-way ANOVA.

RESULTSThere is no difference of GSH level without stimulation between E1A-positive clones and E1A-negative clones. For E1A-positive clones, the level of GSH did not increase in response to H2O2 as E1A-negative clones. The quantitation by densitometry of the NF-kappaB expression in E1A-positive clones were (79.3 +/- 4.6), (80.3 +/- 3.8) respectively without treatment and were (81.8 +/- 3.9) - (89.9 +/- 1.6) and (94.1 +/- 1.9) - (99.8 +/- 1.6) respectively under LPS or TNF-alpha stimulation, which were significantly higher than that of the control group (68.3 +/- 3.8), (69.4 +/- 4.3) respectively without stimulation and (70.1 +/- 2.8) - (80.8 +/- 3.6), (73.4 +/- 4.9) - (83.2 +/- 6.7) respectively under stimulation. The quantitation by densitometry of the NF-kappaB expression in E1A-negative clones were (1.25 +/- 0.18) and (1.69 +/- 0.19) respectively under LPS and TNF-alpha-stimulation and (1.22 +/- 0.16) and (1.75 +/- 0.13) respectively upon treatment for LPS and TNF-alpha with BSO preincubation. There did not show difference upon treatment with LPS or TNF-alpha with or without BSO in E1A-negative cell clone. The quantitation by densitometry of the NF-kappaB expression in E1A-positive clone were (1.75 +/- 0.10) and (2.26 +/- 0.21) respectively upon treatment for LPS and TNF-alpha with BSO preincubation which were significantly higher than that of LPS or TNF-alpha-stimulation alone (1.35 +/- 0.12), (1.80 +/- 0.14) respectively.

CONCLUSIONThese results indicate that E1A protein decreased GSH levels in oxidant stress and upregulated NF-kappaB transcription activity. The oxidant/antioxidant imbalance in rat alveolar epithelial cells enhances E1A-modulated transcriptional activation of NF-kappaB. The mechanism underlying transactivation of NF-kappaB involved by E1A may be related to oxidative stress.

Adenovirus E1A Proteins ; genetics ; metabolism ; Animals ; Cell Line ; Epithelial Cells ; metabolism ; NF-kappa B ; genetics ; metabolism ; Oxidative Stress ; Pulmonary Alveoli ; cytology ; Rats ; Transcriptional Activation

Objective To investigate the clinical efficacy of sertraline combined with famciclovir in treatment of herpes zoster neuralgia . Methods Ninety -four patients with herpes zoster neuralgia were randomly divided into treatment group and control group , 47 cases in each group. Two groups of patients were performed conventional nutritional and physical therapy . Control group was treated with oral administration of famciclovir 0.25 g, tid, treatment group added sertraline 50 mg? d-1 on the basis of control group . The course of treatment was 4 weeks. The visual analogues scale ( VAS ) , clinical efficacy , quality of life and the adverse drug reactions were compared between the two groups.Results In the treatment group , the VAS in 1 and 4 weeks were ( 5.13 ±1.26 ), (3.27 ±1.02), significantly decreased than before treatment ( P<0.05 ) , and significantly lower than control group, which were(6.17 ±1.57) and (4.87 ±1.13).The total effective rate in treatment group ( 93.62%) was higher than control group ( 74.47%, P <0.05 ) .The quality of life in two groups were significantly increased after treatment ( P<0.05 ) , and treatment group significantly higher control group (P<0.05).No serious adverse drug reaction was found in two groups.Conclusion The clinical efficacy of seculin combined with famciclovir in the treatment of herpes zoster neuralgia is significant.

Objective To study the effect ofdifferent doses of dexmedeto-midine to pain , stress and immune function in elderly patients with lapa-roscopic surgery Methods One hundred twenty -six patients of laparo-scopic surgery were randomly divided into low -dose group ( 42 cases ) , middle dose group (42 cases), and high-dose group (42 cases).Be-fore anesthesia 15 min, the low dose group , middle dose group and high dose groups were intravenouslygiven 0.2 , 0.4 , 0.6 μg · kg -1 · h-1 of dexmedetomidine ,respectively.Diastolic bllod pressure ( DBP) , systolic blood pressure ( SBP) , blood sugar ( Glu) , heart rate ( HR) to the hori-zontal and CD4, CD8, CD4/CD8 levels of before and after surgery 3, 8, 24 h were compared.Results The pain scores ,DBP, SBP, Glu and HR levels of before and after surgery 24 h between three groups were not sig-nificant differences ( P>0.05 ).After surgery 3 h and 8 h, pain scores of middle high dose groups were significantly higher than that of low -dose group ( P <0.05 ) and their DBP, SBP, Glu, HR were significantly better than that of low-dose group(P<0.05).Before and after surgery, CD4 levels in three groups of patients did not change significantly.Before and 3 h after surgery, CD8, CD4/CD8 levels in the three groups were not significant difference ( P>0.05 ).After surgery 8 h and 24 h, CD8 levels of high-dose group was significantly lower than of the low -dose group , and the CD4/CD8 ratio was significantly better than low -dose group(P<0.05).Conclusion The use of 0.4, 0.6 μg· kg -1 · h-1 dexmedetomidine scheduled for laparo-scopic surgery in elderly patients have a more significant analgesic effect , can reduce stress and improve immune func-tion in patients , it is worth widely used.

Objective: To explore the protective effect of Baoyuan Qiangshen Capsule No. Ⅱ (BYQS) and its mechanism in treating chronic renal failure (CRF). Methods: Sixty CRF patients were divided into 2 groups randomly, the treated group used BYQS combined with Lotensin and the control group administered with essential amino acid combined with Lotensin. Changes of renal functions and tubular labelled proteins were observed. Results: The markedly effective rate and total effective rate of the treated group were 63.3% and 93.3% respectively, and those of the control group were 30.0% and 56.7% respectively, the effect of the treated group was obviously better than that of the control group (P<0.01). In the treated group after medication, blood urea nitrogen, serum creatinine and clearance rate of creatinine were improved significantly (P<0.01), while Tamm-Horfau protein increased significantly (P<0.01). Conclusion: BYQS could alleviate tubular interstitial injury significantly so as to improve the renal function and enhance the effective rate in treating CRF.

Objective To evaluate the correlation between lesions of dorsal artery of foot and type 2 diabetes on CDFI. Methods Dorsal artery of foot was examinated in 97 cases with type 2 diabetes and 46 cases without diabetes mellitus. Results There were variable changes in intima-media of dorsal artery of foot in type 2 diabetes patients.And the patients with hyperlipidemia and hypertension showed serious lesions in the dorsal artery of foot and bifurcation of the blood vessel .Simple arteriosclerosis showed not only lesions in intima-media of dorsal artery of foot but also relative mild lesions in bifurcation of the blood vessel. Blood vessel lesion incidence in type 2 diabetes was significantly higher than that of the control group (P<0.01). Conclusions There was a significant correlation between lesions of dorsal artery of foot and type 2 diabetes patient's condition. CDFI is an effective method in evaluating patient's condition, degree and prognosis of type 2 diabetes, and has an important clinical value in early diagnosis, prognosis and treatment.Simple arteriosclerosis showed focal lesions in bifurcation of the blood vessel while dorsal artery of foot showed relatively mild lesions .

Transforming growth factor-β1 (TGF-β1)-activated phosphoinositide-3-kinase (PI3K)-protein kinase B (PKB/Akt) pathway is intimately related to the development of diabetic nephropathy (DN), which is negatively regulated by phosphatase and tensin homolog deleted on chromosome ten (PTEN). The present study was to investigate the expression of PTEN in the renal tissue of diabetic mellitus (DM) rats and explore its possible effect on development of DN. Sixteen Sprague-Dawley rats were divided into normal control group (n = 8) and diabetic group (n = 8) at random. Streptozotocin injection was used to establish diabetic model. After 12 weeks, the rats were sacrificed to detect relative biochemical parameters and renal index, and to observe the changes of pathomorphology by HE staining as well. In addition, immunohistochemistry staining and Western blotting were employed to detect the protein expression of PTEN, TGF-β1, PI3Kp110α, Akt1, p-Akt1 (Ser(473)), fibronectin (FN) and Collagen IV, respectively. Furthermore, the expression of PTEN mRNA was also examined by RT-PCR. The results indicated that the levels of blood glucose, serum creatinine and urine protein (24 h) were increased remarkably in the diabetic group (P < 0.05) compared with those in the control group. Compared with those in the control group, the protein expressions of TGF-β1, PI3Kp110α, Akt1 in renal tubular epithelium and the expressions of FN and CollagenIV in renal interstitium were increased in the diabetic group (P < 0.05). The expression of PTEN in the diabetic group was significantly reduced than that in the control group (P < 0.05), and the expression of p-Akt1 (Ser(473)) increased remarkably in the diabetic group which had the similar trend to Akt1 (P < 0.05). PTEN mainly located in renal tubular epithelial cells. The expression of PTEN had negative correlation to that of p-Akt1 (Ser(473)). Compared with that in the control group, the expression of PTEN mRNA was decreased remarkably in the diabetic group (P < 0.05). The data suggest that the down-regulation of PTEN in renal tissue of DM rats may promote the PI3K-PKB/Akt pathway over-activated by TGF-β1, which facilitates the initiation and development of DN.
Animals ; Diabetes Mellitus, Experimental ; complications ; Diabetic Nephropathies ; metabolism ; Down-Regulation ; Kidney ; metabolism ; Male ; PTEN Phosphohydrolase ; genetics ; metabolism ; Phosphatidylinositol 3-Kinases ; metabolism ; Proto-Oncogene Proteins c-akt ; metabolism ; RNA, Messenger ; genetics ; metabolism ; Rats ; Rats, Sprague-Dawley ; Signal Transduction ; Transforming Growth Factor beta1 ; metabolism

OBJECTIVE:To provide reference for pharmacists to participate in the management of chronic disease. METHODS:A total of 259 patients with chronic airway disease [included asthma and chronic obstructive pulmonary disease (COPD)] met the inclusion criteria were selected from our hospital and 5 community health care centers of medical consortium. These patients received medication safety assessment management,which was led by clinical pharmacists of our hospital with the participation of community pharmacists,including medication safety comprehensive evaluation and risk classification management, follow-up and medication guidance, integrated prescriptions checking, establishment of shared database. 1 years after the implementation,the effectiveness were evaluated by score the relatived indicators in related groups. RESULTS:After a year of the management mode practice,compared with before intervention,the patients'safety medication cognitive ability score in high-risk and low-risk group increased from(4.49±1.26)and(7.31±1.01)to(5.40±1.56)and(7.44±0.91);medication adherence score increased from(4.96±1.21)and(7.08±1.24)to(6.66±1.08)and(7.38±0.98);ACT score from asthma patients increased from (16.15±2.58)and(21.15±1.03)to(16.80±2.57)and(21.64±1.55);CAT score from COPD patients decreased from(25.51± 4.07) and (14.90 ± 3.95) to (24.20 ± 3.96) and (13.80 ± 4.08);the rate of irrational prescription effective identification and intervention by pharmacists increased from 3.6％ and 1.4％ to 9.4％ and 7.6％,respectively. All the differences above were statistically significant (P<0.05). CONCLUSIONS:The participation of pharmacists in long-term medication safety assessment management for chronic airway disease patients can improve patients'safety medication cognitive ability,medication adherence, disease control and the pharmacists'ability of irrational drug use identification and intervention.