Objective: To study the relationship between CXCL12/CXCR4 expression and the neural invasion of salivary adenoid cystic carcinoma (SACC). Methods: Totally 41 SACC specimens, 30 tongue cancer specimens, 20 pleomorphic adenoma specimens and 20 normal nerve specimens were included in the present study. The specimens were made into sections of 4 μm and EnVision method was used to detect the expression of the chemokine receptor CXCR4 in SACC sections and the expression of the chemokine CXCL12 in the peripheral nerve tissue sections. Results: The chemokine receptor was highly expressed in ACC cells. The positive rate of CXCR4 was 63.41% in the SACC sections, which was significantly higher than those in the tongue cancer group (36.67%) and the pleomorphic adenoma parotid group (35%) (P<0.05). The expression of CXCL12 was not significantly different between the nerve tissues of SACC, tongue cancer and the normal nerve tissues. Conclusion: The cbemokine CXCL12 is elevated in the SACC cells and its receptor CXCR4 is elevated in the peripheral nerve tissues, indicating a role of the biological axis of CXCL12/CXCR4 in the neural invasion of salivary adenoid cystic carcinoma.

Objective To study effect of octreotide combined with ulinastatin on serum lipase, CRP and clinical efficacy in patients with acute pancreatitis.Methods 60 cases with acute pancreatitis were selected and divided into 2 groups.30 cases in the control group were treated with ulinastatin.30 cases in the experiment group were treated on the base of the control group by octreotide.Clinical effects, lipase and CRP were compared after 1 weeks treatment.Results Compared with the control group after treatment, the serum lipase, CRP level was lower in the experiment group (P<0.05), and the clinical total effective rate was significantly higher in the experiment group than in the control group ( P<0.05 ) . Conclusion Octreotide combined with ulinastatin has good clinical curative effect on acute pancreatitis.It is speculated that the mechanism may be related to the decreasing of serum lipase and CRP level in patients.

Objective To explore the change and its significance of blood concentrations of high sensitive C-reactive protein(hs-CRP) and D-Dimer in patients with acute cerebral infarction caused by internal carotid artery.Methods The blood levels of D-Dimer and hs-CRP in 69 patients with acute cerebral infarction were measured,and compared with the normal controls.The relationships between the levels of hs-CRP and D-Dimer and NIHSS or the areas of infarction were analyzed.Results Compared with those in normal control group,the blood levels of D-Dimer and hs-CRP in patients with acute cerebral infarction increased significantly(all P

Objective Up to now, the role of Brucea in early embryonic development of mice and its mechanism is still unclear.This paper aims to explore the role of Brusatol in mouse early embryonic development and its possible mechanism.Methods 100 kunming rats of clean grade(80 female rats and 20 male rats) were divided into 6 group: negative control group(no DMSO)、blank control group(culture in fresh CM with equal DMSO)、20nmol/L brusatol treated group、50nmol/L brusatol treated group、100nmol/L brusatol treated group、200nmol/L brusatol treated group(A solution of Brusatol was diluted in CM to concentrations of 20, 50, 100 or 200nmol/L.).Each group used an average of 20 embryos each time, repeated 4 times.Fertilized eggs after cultured 24h, 48h,72h, 96h were respectively 2-cell stage, 4-cell stage,morula and blastocyst stage..The embryo development rate was observed in the culture medium and the optimal concentration was selected, the embryos were collected to analysis the subcellular localization of the Nrf2 by immunofluorescence.The mRNA expression level of Cyclin B, CDK1 and the protein expression of Nrf2 were detected by Q-PCR and western blot respectively.Results In 4-cell stage, the embryo development rates of 20、50、100nmol/L brusatol treated groups[(75.0±2.8)%、(30.4±7.5)%、(4.2±5.9)%] significantly reduced compared with the negative control group[(93.0±2.8)%]、blank control group[(90.9±1.2)%].In morula stage, compared with blastocyst rates of negative control group、blank control group [(83.5±2.1)%、(84.2±1.2)%], 50nmol/L brusatol treated group[(19.3±13.1)%] decreased obviously [(79.00±0.06)% vs 100%, P<0.05].In the cellular immunofluorescence assay, the expression of Nrf2 protein in 50nmol/L brusatol treated group was lower than blank control group(P<0.05).We further found that 50nmol/L brusatol treated group decreased more mRNA levels of Cyclin B[(59.5±9.2)%] and CDK1[(56.0±1.4)%] than blank control group(100%) in G2/M phase(P<0.05).Conclusion In this study, Brusatol mainly affects the cell cycle transformation from G2 to M phase dependent on Cyclin B-CDK1, further inhibiting the development of the embryo through down-regulating Nrf2.

The new medical model which is more emphasis on patient-centered concept and the patient's state of mind and mental health has brought a lot of new challenges to medical students who are about to become the medical workers while also put forward new tasks to the medical educational workers.Medical professionalism and doctor-patient communication are very important for the students to handle.Medical professionalism is the professional guidelines of the doctors,which is an important guarantee for the interests of the patients and the embodiment of noble character of doctors.Doctor-patient communication is basal to create a harmonious doctor-patient relationship and is a key means to ensure the medical quality.In addition to lay a solid theoretical foundation,medical students should also learn to establish correct medical professionalism and grasp effective communication skills to become qualified doctor.

The Nipah virus outbreak in Malaysia (September 1998 to May 1999) resulted in 265 cases of acute encephalitis with 105 deaths, and near collapse of the billion-dollar pig-farming industry. Because it was initially attributed to Japanese encephalitis, early control measures were ineffective, and the outbreak spread to other parts of Malaysia and nearby Singapore. The isolation of the novel aetiological agent, the Nipah virus (NiV), from the cerebrospinal fluid of an outbreak victim was the turning point which led to outbreak control 2 months later. Together with the Hendra virus, NiV is now recognised as a new genus, Henipavirus (Hendra + Nipah), in the Paramyxoviridae family. Efforts of the local and international scientific community have since elucidated the epidemiology, clinico-pathophysiology and pathogenesis of this new disease. Humans contracted the infection from close contact with infected pigs, and formed the basis for pig-culling that eventually stopped the outbreak. NiV targeted medium-sized and small blood vessels resulting in endothelial multinucleated syncytia and fibrinoid necrosis. Autopsies revealed disseminated cerebral microinfarctions resulting from vasculitis-induced thrombosis and direct neuronal involvement. The discovery of NiV in the urine and saliva of Malaysian Island flying foxes (Pteropus hypomelanus and Petropus vampyrus) implicated these as natural reservoir hosts of NiV. It is probable that initial transmission of NiV from bats to pigs occurred in late 1997/early 1998 through contamination of pig swill by bat excretions, as a result of migration of these forest fruitbats to cultivated orchards and pig-farms, driven by fruiting failure of forest trees during the El Nino-related drought and anthropogenic fires in Indonesia in 1997-1998. This outbreak emphasizes the need for sharing information of any unusual illnesses in animals and humans, an open-minded approach and close collaboration and co-ordination between the medical profession, veterinarians and wildlife specialists in the investigation of such illnesses. Environmental mismanagement (such as deforestation and haze) has far-reaching effects, including encroachment of wildlife into human habitats and the introduction of zoonotic infections into domestic animals and humans.
Swine ; Virus ; Malaysia ; seconds ; control

Objective:To investigate the protective effect of N-acetylcysteine(NAC)on right ventricular remodeling in a rat model of monocrotaline-induced pulmonary arterial hypertension ( PAH ) .Methods: PAH rats were induced by a single injection of monocrotaline(60 mg/kg,sc)and were ad ministered with NAC[500 mg/(kg? d)]for 6 weeks.At the end of 4 weeks,the right ventricular systolic pressure ( RVSP ) and mean pulmonary artery pressure ( mPAP ) were monitored via the right jugular vein catheterization into the right ventricle .Right ventricle ( RV ) to left ventricle ( LV )+septum ( S ) were calculated.Right ventricular morphological change was observed by HE staining .Sirius red was used to demonstrate collagen deposition .The expressions of collagenⅠ,collagen Ⅲ, NADPH oxidase 4 ( NOX4 ) and nuclear factor-kappa B ( NF-κB ) were analyzed by RT-PCR and ( or ) Western blot.Results:NAC attenuated RVSP ,mPAP and right ventricular remodeling index ( RV/LV+S) of PAH rats induced by monocrotaline after treatment for 4 weeks.Furthermore ,monocrotaline-induced right ventricular collagen accumulation and collagen Ⅰand collagenⅢexpression were both significantly suppressed by NAC .The expressions of NOX 4 and NF-κB were obviously decreased in right ventricule from PAH rats with NAC treatment.Conclusion:NAC ameliorates right ventricular remodeling of PAH induced by monocrotaline in rats through down regulating the expression of NOX 4 and antioxidant activity ,and inhibiting activation of NF-κB and collagen accumulation .

Objective To investigate the clinical safety of preoperative lymphatic chemotherapy in the treatment of reetal cancer.Methods The regional and systemic symptoms,postoperatwe stoma healing,haematogenesis.functions of hean,liver and kidney after lymphatic chemotherapy,and the level of CD3+,CD4+,CD8+,CD4+/CD8+,CD(16+56)+in blood 30 minutes before and 48 hours after lymphatic chemotherapv were detected.Results There were no significant effects of lymphatic chemotherapy on the regional and systemic symptoms,postoperative stoma healing,haematogenesis and the functions of heart,liver and kidney.The level of CD4+/CD8+48 hours after lymphatic chemotherapy was significantly increased(t=7.145,P＜0.05),while no significant changes of CD3+,CIM+,CD8+,CD(16+56)+were detected(t=1.782,1.151,1.184,0.955,P＞0.05),when compared with those 30 minutes before lymphatic chemotherapy.Conclusions Preoperative lymphatic chemotherapy is safe and can enhance patients'immunity in early stage.

Objective To evaluate the role of delta and kappa opioid receptors in sufentanil preconditioning (SPC)-induced attenuation of myocardial ischemia-reperfusion (I/R) injury in rats.Methods Forty adult male Sprague-Dawley rats,aged 2-3 months,weighing 250-330 g,were randomly divided into 5 groups (n =8 each) using a random number table:sham operation group (group S),group I/R,group SPC,δ receptor antagonist naltrindole + SPC group (NTD + SPC group),and κ receptor antagonist nor-binaltorphimine (nor-BNI) + SPC group (BNI + SPC group).Myocardial I/R was induced by 30 min occlusion of left anterior descending branch of coronary artery followed by 120 min reperfusion.In group SPC,5 min infusion of sufentanil 3μg/kg was repeated 3 times at 5 min interval before myocardial ischemia.In NTD + SPC group,naltrindole 5 mg/kg was intravenously injected,at 10 before SPC.In BNI + SPC group,nor-BNI 5 mg/kg was injected intravenously at 15 min before SPC.Arterial blood samples were collected at 120 min of reperfusion for measurement of the serum cardiac troponin I (cTnI) and creatine kinase isoenzyme-MB (CK-MB) concentrations.The rats were then sacrificed and hearts removed for determination of myocardial infarct size (IS) and area at risk (AAR).IS/AAR ratio was calculated.Results Compared with S group,the serum cTnI and CK-MB concentrations were significantly increased in I/R,NTD + SPC and BNI + SPC groups,and the serum CK-MB concentrations were increased in SPC group,and IS/AAR ratio was increased in I/R group (P ＜ 0.05 or 0.01).The serum cTnI and CK-MB concentrations were significantly lower in SPC,NTD + SPC and BNI + SPC groups and IS/AAR ratio was lower in SPC and BNI + SPC groups than in I/R group,and higher in NTD + SPC and BNI + SPC groups than in SPC group (P ＜ 0.05 or 0.01).Conclusion Both κ and δ opioid receptors mediate SPC-induced attenuation of myocardial I/R injury in rats.

Traumatic brain injury(TBI), also known as intracranial injury or head trauma, specifically refers to the brain tissue damage caused by trauma.Currently the mechanism of TBI and repair therapy after nerve injury become a hotspot in brain research.Duplicating animal models plays a significant role in promoting experimental therapeutics of TBI.This review systematically describes the progress in animal models for TBI including impact brain injury, nonimpact acceleration head injury and blast(explosion)wave-induced neurotrauma, which have been established at home and abroad.Based upon the aforementioned models, some relevant applications in experimental therapeutics are simultaneously enumerated.Hopefully all these information provides scientific guidance for the pharmacodynamic screening of potential neuroprotective drugs.