Objective To observe the dynamic changes of myocardial structure and dysfunction during post-resuscitation period in order to establish a rat mode of post-resuscitation myocardial dysfunction after cardiac arrest resulted from electric stimulation-induced ventricular fibrillation (VF) and cardiopulmonary resuscitation (CPR).Methods A total of 40 male Sprague-Dawley (SD) rats were randomly (random number) assigned into post-resuscitation (PR) 4 h,PR 12 h,PR 24 h,PR 72 h and sham groups.VF was induced by an alternating electric current delivered to the right ventricular endocardium and untreated for 8 min.Biphasic waveform defibrillation was attempted and mechanical ventilation was synchronized after 6 min of CPR.Myocardial function was assessed with serum myocardial enzyme activity,echocardiography,mitochondrial respiratory function and histopathologic findings at different intervals.Results Thirty-two animals were successfully resuscitated with restoration of spontaneous circulation (ROSC) in 86％ (32/37) rats.Compared with sham group,severe systolic and diastolic heart failure were found at 4 h after ROSC and then gradually improved without significant difference (P ＞0.05) in ejection fraction at PR 72 h after ROSC was found,whereas thickened ventricular wall and increased myocardial performance index as well as interstitial proliferation were observed at 72 h after ROSC.Conclusions A rat model of post-resuscitation myocardial dysfunction after cardiac arrest resulted from electric stimulation-induced VF and CPR was successfully established.

BACKGROUND: It is necessary to establish a high throughput screening system for anti -inflammatory drugs for rheumatoid arthritis.OBJECTIVE: To construct an eukaryotic expression vector p4CCL20-ZsGreen1-DR with the NF-kB cis-acting element 4×CCL20motif as an enhancer, SV40 as a promoter, and ZsGreen1-DR as a reporter gene.METHODS: The target fragment SV40 was PCR amplified using PGL2-control plasmid as a template. KpnⅠ/Bam HⅠ restriction sites were introduced into the flank of the target fragment. Then, pSV40-ZsGreen1-DR vector was constructed by cloning the target fragment into pZsGreen1-DR plasmid. Finally, p4CCL20-ZsGreen1-DR plasmid was constructed by cloning the double strand DNA of 4×CCL20 motif (with BglⅡ and EcoRⅠ sticky ends at the 5’ and 3’ terminus, respectively) into the corresponding restriction sites of pSV40-ZsGreen1-DR vector (upstream of SV40 promoter).RESULTS AND CONCLUSION: DNA sequencing demonstrated successful construction of p4CCL20-ZsGreen1-DR plasmid.The construction of p4CCL20-ZsGreeR plasmid might be useful to establish a high throughput screening system for anti -inflammatory drugs.

Objietive Observe the clinical curative effect of PPH in treatment of complexity hemorrhoids and circular hemorrhoid. Methods From January 2010 to January 2014, the clinical data of 278 patients who underwent PPH to treat complexity hemorrhoids, mixed hemorrhoid and circular hemorrhoid were retrospectively analyzed. The postoperative recent and forward curative effect was observed. Results The average operativetime was 28 minutes, and the average hospital stay were 6. 1 days. There were postoperative complications such as pain, anal skin edema, bleeding and urinary retention. 186 cases were cured (66. 9%),35 cases were markedly improved (12. 6%),20 ca-ses were of poor effect (7. 2%),and recurrence occured in 37 cases (13. 3%). Conclusion PPH has the advantages of simple in operation and quick in postoperative recovery. But there were to many postoperative complications and the recurrence rate is high. So PPH needs more further observation and study.

Objective To observe the clinical efficacy of deproteinized calf blood extractive injection combined with hyperbaric oxygen in patients with severe traumatic brain injury.Methods 74 patients with severe traumatic brain injury from January 2013 to March 2015 in Wenzhou hospital of Chinese traditional medicine were randomly divided into observation group and control group, 37 cases in each group.The control group received hyperbaric oxygen on the basis of conventional therapy, the observation group received deproteinized calf blood extractive injection on the basis of control group.The Glasgow coma scale ( GCS) , Barthel, hs-CRP, TNF-α, IL-6 and prognosis were compared between two groups.Results The GCS score and Barthel index scores post-treatment in observation group were (13.67 ±1.73),(65.73 ±4.02) points, which were higher than (9.66 ±1.24), (50.69 ± 3.76) points in control group, and the difference was significant (P<0.05).The serum hs-CRP, TNF-α, IL-6 post-treatment in observation group were (4.55 ±0.76)mg/L,(1.21 ±0.05)μg/L,(0.21 ±0.01)μg/L, which were better than those of control group (6.43 ±1.01)mg/L,(1.36 ±0.06)μg/L,(0.28 ±0.02)μg/L (P<0.05).The rate of favorable prognosis in observation group was 48.65%, which was higher than that of control group (P<0.05).Conclusion The deproteinized calf blood extractive injection combined with hyperbaric oxygen has the exact efficacy, which was better than hyperbaric oxygen alone in the treatment of severe traumatic brain injury.

OBJECTIVETo investigate the effects of copper on permeability and P-glycoprotein (P-gp) of Caco-2 cell monolayers.

METHODSThe differentiated Caco-2 cell model was used in this study. Permeability of cell monolayers was reflected by monitoring transepithelial electrical resistance (TEER); distribution of tight junctional protein ZO-1 was measured by immunofluorescent staining; F actin was measured by fluorescence staining; and Activity of P-gp was reflected by changes of transcellular transport and accumulation of Rho-123 in Caco-2 cells.

RESULTSApical treatment with copper (30 - 100 micromol/L, Hanks' buffered salt solution, up to 3 hours) induced a time- and concentration-dependent increase in permeability reflected by progressive decrease of TEER of Caco-2 cell monolayers, accompanied by deorganization of F actin, but without significant effects on tight junctional protein ZO-1; at a dose without any adverse effects on viability and permeability of Caco-2 monolayers, copper treatment (300 micromol/L, complete medium, 24 hours) decreased Papp(BL-->AP) from 7.37 +/- 0.20 x 10(-6) cm/s (controls) to (6.43 +/- 0.27) x 10(-6) cm/s, the increased Papp(AP-->BL) from (1.23 +/- 0.05) x 10(-7) cm/s (controls) to (3.41 +/- 0.08) x 10(-7) cm/s, and enhanced the intracellular Rho-123 from (0.31 +/- 0.01) nmol/filter (controls) to (0.50 +/- 0.03) nmol/filter.

CONCLUSIONCopper might alter the barrier functions of Caco-2 cells through increasing the permeability and inhibiting P-gp of Caco-2 cells.

ATP-Binding Cassette, Sub-Family B, Member 1 ; metabolism ; Biological Transport ; Caco-2 Cells ; Cell Membrane Permeability ; drug effects ; Copper ; toxicity ; Humans

OBJECTIVETo evaluate the loss of disability adjusted life years (DALYs) due to particulate air pollution in Chinese urban residents.

METHODSDALYs was applied to evaluate the health effects due to particulate air pollution in the residents of 656 Chinese cities, and the data of annual average concentrations of inhalable particle (PM(10)) in 2006 were collected from the state-owned air quality monitoring network.

RESULTSParticulate air pollution in Chinese urban areas in 2006, could cause (506.6 +/- 95.2) thousand premature deaths, (156.6 +/- 41.2) thousand new cases of chronic bronchitis, (12.6 +/- 5.2) million outpatient visits for internal medicine, (99.9 +/- 50.4) thousand and (72.0 +/- 8.2) thousand hospital admissions for cardiovascular diseases and respiratory diseases respectively. The attributable DALYs loss in urban residents amounted up to (5.26 +/- 0.99) million person years in 2006, of which, 96.26% (506.55/526.22) were caused by premature deaths. The attributable loss of DALYs increased with more seriously PM-polluted atmosphere and larger urban population.

CONCLUSIONSubstantial adverse health effects due to outdoor particulate air pollution were observed in China urban areas, thus strengthening air pollution control still remains an imperative and urgent matter in public health perspective.

Air Pollutants ; adverse effects ; Air Pollution ; adverse effects ; China ; Cities ; Environmental Exposure ; adverse effects ; Environmental Monitoring ; Health Status ; Humans ; Life Tables ; Particulate Matter ; adverse effects ; Risk Assessment

OBJECTIVETo obtain the exposure-response functions that could be used in health-based risk assessment of particulate air pollution in China.

METHODSMeta analysis was conducted on the literatures on air particulate matter and its adverse health outcomes in China and worldwide.

RESULTSFor each health outcome from morbidity to mortality changes, the relative risks were estimated when the concentration of air particulate matter increased to some certain units.

CONCLUSIONThe exposure-response functions recommended here can be further applied to health risk assessment of air particulate matter in China.

Acute Disease ; Adult ; Air Pollutants ; adverse effects ; toxicity ; Asthma ; epidemiology ; etiology ; Bronchitis ; epidemiology ; etiology ; Bronchitis, Chronic ; epidemiology ; etiology ; Cardiovascular Diseases ; epidemiology ; etiology ; Child ; China ; Dust ; Environmental Exposure ; Hospitalization ; Humans ; Mortality ; Particle Size ; Risk ; Risk Assessment

OBJECTIVETo investigate the association between temperature and daily mortality in Shanghai from June 1, 2000 to December 31, 2001.

METHODSTime-series approach was used to estimate the effect of temperature on daily total and cause-specific mortality. We fitted generalized additive Poisson regression using non-parametric smooth functions to control for long-term time trend, season and other variables. We also controlled for day of the week.

RESULTSA gently sloping V-like relationship between total mortality and temperature was found, with an optimum temperature (e.g. temperature with lowest mortality risk) value of 26.7 degrees C in Shanghai. For temperatures above the optimum value, total mortality increased by 0.73% for each degree Celsius increase; while for temperature below the optimum value, total mortality decreased by 1.21% for each degree Celsius increase.

CONCLUSIONSOur findings indicate that temperature has an effect on daily mortality in Shanghai, and the time-series approach is a useful tool for studying the temperature-mortality association.

China ; Environmental Monitoring ; Humans ; Mortality ; trends ; Regression Analysis ; Temperature ; Urban Population

OBJECTIVETo evaluate the impact of long-term air particulate matter exposure on the life expectancy and survival rate of Shanghai residents.

METHODSEpidemiology--based exposure-response function was used for the calculation of attributable deaths to air particulate matter in Shanghai, and the effect of long-term exposure to particulate matter on life expectancy and survival rate was estimated using the life table of Shanghai residents in 1999.

RESULTSIt was shown that in 1999, the long-term air particulate matter exposure caused 1.34-1.69 years reduction of life expectancy and a decrease of survival rate for each age group of Shanghai residents.

CONCLUSIONThe effect of long-term exposure to air particulate matter on life expectancy is substantial in Shanghai.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Air Pollutants ; adverse effects ; Child ; Child, Preschool ; China ; Environmental Exposure ; Female ; Humans ; Infant ; Infant, Newborn ; Life Expectancy ; Male ; Middle Aged ; Particle Size ; Survival ; Urban Population

OBJECTIVETo study the reversibility of di-n-butyl phthalate (DBP) effects on F(1) generation rat testes.

METHODSPregnant rats were treated with different dose of DBP (0, 50, 250 and 500 mg per kg per day) by gavage from GD1 to PND21. The adverse effects of DBP on testes of F(1) male rats in different developmental period (PND14, 21 and 70) were observed by anatomy and pathological methods.

RESULTSThere was no difference in rat testis weight and testis/body weight between DBP-treated group and the control. From the results of pathology and sertoli cell counting, comparing with the control, thinner seminiferous epithelium, decreased cell number and vacuole cells were observed in PND14 male DBP-treated rats. In PND21 rats, the number and form of sertoli cells were recovered and few exfoliated spermatogenic cells were found. When maturing to PND70, few rats were found irreversible damages such as seminiferous tubule degeneration, seminiferous epithelium atrophy, etc.

CONCLUSIONThese results suggest that adverse effects of DBP on young rat testes should be reversibility.

Animals ; Dibutyl Phthalate ; toxicity ; Female ; Male ; Rats ; Rats, Sprague-Dawley ; Sertoli Cells ; pathology ; Testis ; drug effects ; pathology