1.The impact of rh-LIF on HL-60 cell line
Journal of Chongqing Medical University 1986;0(03):-
Objective:To investigate the effect and mechanism of rh-LIF on proliferation and differentiation of HL-60 cell line.Methods:HL-60 cells were treated with different dosage of rh-LIF for 3 to 6 days,cell cycle was analysed by flow cytometry,expression of P53 and P21 protein were observed by immunohistochemistry and P53 mRNA were assayed by in situ hybridization.Results:HL-60 cell line could differentiate into monocyte and macrophage cell line in the presence of rh-LIF.The proliferation of HL-60 cells were inhibited in the presence of rh-LIF,the cells stagnated at G 1 stage,while expression of P53,P21 protein and P53 mRNA were increased obviously.Conclusion:The effects of rh-LIF on the proliferation and differentiation of HL-60 cells may be associated with the increase of P53,P21 protein and P53mRNA.
2.Study of Reversing Effect of Nitric Oxide Precursor on Collagen Accumulation in Pulmonary Artery
China Pharmacy 2001;0(11):-
OBJECTIVE:To study the therapeutic effect of L-arginine on collagen metabolism of pulmonary artery in rats with high pulmonary blood flow METHODS:The rat model of pulmonary hypertension was established with an abdominal aorta and inferior vena cava shunting L-arginine was intragastrically given to the rats with shunt in L-arginine group(1g/(kg?d)) for 11 weeks After 11 weeks of experiment,the expressions of collagen Ⅰ and collagen Ⅲ in pulmonary artery were detected by immunohistochemical assay RESULTS:The expressions of collagen Ⅰ and collagen Ⅲ in shunt group elevated obviously compared with those in control group(P
4.Survey of knowledge and teaching demand of medical statistics among doctoral students in a medical university
Shengli AN ; Bing NING ; Yue DU
Journal of Medical Postgraduates 2015;(9):962-965
Objective Medical statistics is an important tool in scientific research and practice.The article was to evaluate the current knowledge, learning needs and related influencing factors of medical statistics among medical doctoral students to provide references for the optimization in the teaching mode of medical statistics. Methods A questionnaire was used to investigate Grade 2014 doctoral students who took medical statistics course before and after the teaching in a medical university. Results The knowl-edge of doctoral students were at different levels and they had different learning requirements.More than 81.4% of the respondents have difficulty in the choice of scientific and reasonable design and the scientific design.Over 50%students had insufficient knowledge of advanced statistics and poor software application ability.They had the demands for more practical cases explanation in theory course, more software teaching hours and some short term specialized courses. Conclusion The university and teachers should carry out teaching reform according to the particularity of doctoral students and teach in small sections according to their knowledge levels of sta-tistics and learning requirements.It is suggested that the teaching should be combined with the students'own research projects in order to improve the teaching effect and improve the students'ability in sta-tistical design and solving the practical problem in scientific research statistics.
5.Survey on the current situation of community home endowment nursing services in Yancheng City
Haiyan SUN ; Feng WANG ; Bing DU
Chinese Journal of Practical Nursing 2014;30(8):4-7
Objective To investigate the situation of implementation of community home endowment nursing services in Yancheng City to provide basis for developing nursing services for the elderly.Methods Survey was conducted for the 255 elderly in 15 aged-service stations.We used the multistage random sampling by questionnaire and interview.Results There was difference in the need of community home endowment service,the situation of implementation,and in the service evaluation.Conclusions Nursing service for old people in community home endowment is still developing in elementary step.We should establish the normative and exercisable quality specifications and evaluation systems of nursing service for old people,in order to assure quality of nursing service.
6.Clinical study: the effects of inhaling nitrous oxide for analgesia labor on pregnant women and fetus
Xudong OU ; Bing LI ; Hongzi DU
Chinese Journal of Obstetrics and Gynecology 2001;0(07):-
Objective To investigate the effect of the inhalation of nitrous oxide premixed with oxygen (50%∶50%) for analgesia labor on maternal and fetus. Methods A total of 100 cases of pregnant women were provided with nitrous oxide premixed with oxygen (50%∶50%) (control group); Another 100 cases were provided only with oxygen (comparison group). Recording duration of the Labor, way of delivery, bleeding volume, Apgars score, blood gas analysis to maternal radius artery and fetal umbilical blood among all patients. Results The effect for analgesia labor of the premixed gas was much better than that of control group, but there were no significant differences in time of labor, bleeding volume, Apgars score between the two groups. Conclusions The inhalation of nitrous oxide premixed with oxygen (50%∶50%) for analgesia labor benefits pregnant women because of keeping them being a good mental and physical condition. The inhalation of nitrous oxide for analgesia labor is a safe, effective and easy method.
7.Effects of Tenglong Buzhong Decoction on proliferation and apoptosis of human colon carcinoma cell line LS174T.
Bing HU ; Hongmei AN ; Keping SHEN ; Qin DU
Journal of Integrative Medicine 2010;8(6):575-80
Objective: To observe the effects of Tenglong Buzhong Decoction (TLBZD), a compound traditional Chinese herbal medicine, on proliferation and apoptosis of colon carcinoma cell line LS174T in vitro. Methods: Human colon carcinoma cell line LS174T and human colon epithelial cell line CRL-1790 were treated with different doses of TLBZD. Cell proliferation was detected with cell counting kit-8 (CCK-8) assay and clone formation assay. Cell cycle and apoptosis were detected by flow cytometry, and caspase-3, -8 and -9 activities in LS174T cells were detected by colorimetric assay. Results: TLBZD had no obvious cytotoxicity in normal CRL-1790 cells. After 72-hour treatment of 1 mg/mL TLBZD, or 48- and 72-hour of 2 mg/mL TLBZD, or 24-, 48- and 72-hour of 5-20 mg/mL TLBZD, proliferation of LS174T cells was significantly inhibited. Clone formation of LS174T cells was significantly inhibited by 1 to 20 mg/mL TLBZD treatment. TLBZD at doses of 5 to 20 mg/mL also induced apoptosis and cell cycle arrest at G(0)/G(1) phase in LS174T cells. In addition, caspase-3, -8 and -9 activities were significantly elevated after 5 to 20 mg/mL TLBZD treatment. Conclusion: TLBZD can inhibit cell proliferation, arrest cell cycle at G(0)/G(1) phase, and induce apoptosis in LS174T cells, which may be related to activating of caspase-3, -8 and -9.
8.Mata analysis on arotinolol in treatment of essential hypertension
Bing DU ; Ling QIN ; Wenpeng CUI
Journal of Jilin University(Medicine Edition) 2006;0(05):-
Objective To approach the efficacy and safety of arotinolol in treating essential hypertension.Methods The studies about arotinolol in treating essential hypertension were accessed by searching the Cochrane Central Register of Controlled Trials(Issue 3,2008),MEDLINE(1991 to March 2009),EMbase(1991 to March 2009),CBMdisc(1991 to March 2009),and CNKI(1994 to March 2009).The relevant journals and conference proceedings also hand searched.Randomized controlled trials(RCTs) in which arotinolol was used to treat patients with essential hypertension were collected.Then the retrieved studies according to predefined inclusion and exclusion criteria were screened,the quality of included studies was evaluated,and Meta analysis was performed by using RevMan 4.2 software.Results A total of 176 articles were found and 6 of which were finally included.In homogeneity test:?2=4.41,df=7,P=0.73(efficacy);?2=2.96,df=4,P=0.56(safety).In combined test,Z=0.64(P=0.52),OR=1.17,OR95%CI(0.72-1.85)(efficacy);Z=1.75(P=0.08),OR=0.60,OR95%CI(0.34-1.06)(safety).Conclusion There is no significant difference in efficacy and safety between arotinolol and control group in treating essential hypertension.
9.Laparoscopic repair of abdominal wall incisional hernia:A report of 41 cases
Wen TIAN ; Bing MA ; Xiaohui DU
Chinese Journal of Minimally Invasive Surgery 2005;0(09):-
Objective To investigate effects of laparoscopic repair of abdominal wall incisional hernia using polypropylene and expanded polytetrafluoroethylene(e-PTFE) composite mesh. Methods Forty-one patients with abdominal wall incisional hernia(4~25 cm in length and 3~18 cm in width) were treated in this hospital from October 2004 to August 2005. The patients received laparoscopic mesh herniorrhaphy after complete dissection of adhesion using an ultrasonic scalpel.A polypropylene and(e-PTFE) composite mesh(Bard Composite Mesh) was used and fixed using the Ethicon Endopath Multifeed Stapler(EMS).Results The laparoscopic mesh herniorrhaphy was successfully completed in all the 41 patients without conversions to open surgery.The operative time was 60~182 min(mean,85 min).Postoperatively,the patients felt slight pain and began to take food on the second day.The time to first passing flatus was 25~41 hours(mean,32 hours).The postoperative hospital stay was 5~7 days(mean,6 days).No recurrence occurred during a follow-up period of 6~16 months(mean,9 months) in the 41 patients. Conclusions The laparoscopic incisional hernia repair using the Bard Composite Mesh is a safe and effective method.
10.Endoscopic thyroidectomy through anterior chest wall
Jingping DU ; Bing PENG ; Yongqiong TAN
Chinese Journal of Minimally Invasive Surgery 2001;0(03):-
Objective To study the possibility of endoscopic thyroidectomy. Methods Benign thyroid tumors, with diameter between 22 mm and 38 mm, were successfully removed in 8 patients by endoscopic thyroidectomy through anterior chest wall. Results The mean operation time was 120 min (range, 60 min~180 min), and the mean intraoperative blood loss was 20 ml (range, 10 ml~50 ml). No intraoperative or postoperative complications occurred. And no scars remained. Conclusions Endoscopic thyroidectomy through anterior chest wall is a safe and effective procedure for benign thyroid tumors, with excellent cosmetic results in the neck postoperatively.