Ischemia-reperfusion after neonatal asphyxia is a key factor in renal injury,which often can lead to apoptosis of tubular epithelial cells.Apoptosis is an important form of injury for renal tubular epithelial cells after asphyxia.Large number of cancer genes involve in regulation of renal ischemia-reperfusion injury in the process of apoptosis.Bcl-2 protein which are expression products of Bcl-2 oncogene act on the mitochondrial pathway in apoptosis.Furthermore they can inhibit the caspase cascade of apoptosis via the cells "cross-talk",which contribute to attenuate renal ischemia-reperfusion injury and improve renal function.

Objective To explore the effects and mechanism of propofol on cognitive function of type 2 diabetic rats.Methods Ten of fifty adult male SD rats were fed with basic diet and allocated to control group.Another forty rats were fed with high sugar and high fat for 8 weeks and composite intraperitoneal injection of 1% streptozotocin (STZ)to establish model and then divided into four groups:diabetes group;low dose,middle dose and high dose of propofol group (diabetic rats were given intraperitoneal injection of 1% propofol 10,30,75 mg·kg-1·d-1 for 5 consecutive days).The cognitive functions were examined by Morris water maze from the first day after intraperitoneal injec-tion with propofol.The hippocampus were isolated for observing histopathologic alterations by HE staining and for the determinations of SOD,MDA,CAT,GSH and GSH-PX by colorimetry. Western blot was used to detect the expression of AGEs and RAGE.Results Compared to the control group,there was an obvious increased escape latent period,decreased the frequency of crossing platform,increased hippocampal neurons damage and MDA,decreased levels of SOD, CAT,GSH and GSH-PX,as well as the protein levels of AGEs and RAGE in diabetes group (P <0.05).There was no significant difference between diabetes group and low dose propofol of group on behavior ability and detection index.However,middle dose and high dose of propofol group showed more serious cognitive dysfunction,aggravated hippocampal neurons cells loss,increased oxidative stress as well as enhanced expression of AGEs and RAGE (P <0.05 ).Conclusion Multiple given sedative or anesthetic doses of propofol can aggravate the cognitive dysfunction and oxidative stress in type 2 diabetic rats,which may be related to increase the expression of AGEs and RAGE in brain tis-sue.

Education, Dental ; Oral Medicine ; education ; manpower

ObjectiveTo investigate the effects of dexmedetomidine on postoperative cognitive dysfunction (POCD) in the elderly patients undergoing laparoscopic radical trachelectomy.MethodsSixty ASA Ⅰ or Ⅱ patients (aged 65-80 yr and weighing 55-74 kg) undergoing elective laparoscopic radical trachelectomy were randomly divided into two groups:30 patients received intravenous injection of dexmedetomidine 0.2 μg · kg - 1 ·h- 1 ( group D EX) and 30 received same volume of normal saline (control group,group NS) after induction of anesthesia.Cognitive function was assessed at 1 day before operation and on the 7th day after operation using a battery of nine cognitive dysfunction tests.ResultsCompared with group NS,on the 7th postoperative day,the incidence of POCD was decreased in group DEX ( P ＜ 0.05 ).ConclusionDexmedetomidine can improve POCD in the elderly patients undergoing laparoscopic radicaltrachelectomy.

BACKGROUND: Exercise preconditioning can lighten exercise-induced muscle damage, thereby to avoid delayed onset muscle soreness. At present, experimental research is scarce that apply intedeukin-6 (IL-6), creatine kinase (CK) and creatine kinase isoenzyme (CK-MM) to evaluate skeletal muscle damage.OBJECTIVE: To observe the effects of exercise precondiUoning on muscle damage at different phases after eccentric exercise and changes of blood IL-6, CK and CK-MM.DESIGN, TIME AND SETTING: The randomized control animal expedmant was carded out in the Animal Laboratory of Chengdu Sports University between 2006 and 2007. MATERIALS: Eighty female adult SD rats, weighing (231.3+12.44) g, were adopted. Eighty rats were randomly divided into without exercise preconditioning group (n=40) and exercise preconditioning group (n=40). Each group was assigned into 5 subsets, termed before exercise, immediately after exercise, 24, 48, 72 hours after exercise, with 8 rats in each subset. METHODS: Except before exercise subset, other rats in the without exercise preconditioning group were forced to do treadmill exercise (19-21 m/min, -16° incline, 90 minutes). All rats of exercise preconditioning group were forced to do eccentric treadmill exercise for two weeks. After two weeks, treadmill test was made for rats except before exercise subset. MAIN OUTCOME MEASURES: Soleus muscle structure, blood IL-6, CK and CK-MM immediately, 24, 48, 72 hours after eccentric exercise.RESULTS: The soleus muscle was damaged after exercise, especially in without exercise preconditioning group at 24-48 hours after exercise. Blood IL-6 of without exercise preconditioning group increased significantly immediately after exercise and then gradually decreased, but again raised at 72 hours after exercise. In the exercise preconditioning group, blood IL-6 slightly reduced immediately after exercise and then gradually increased. Peak value appeared at 48 hours. After exercise, IL-6 of exercise preconditioning group was obviously lower than that of without exercise preconditioning group. Before exercise, serum CK and CK-MM of exercise preconditioning group were less than that of without exercise preconditioning group. After exercise, the CK and CK-MM were firstly raised and then reduced in two groups. Except 72 hours after exercise subset, the variation of CK and CK-MM of exercise preconditioning group was lower than that of without of exercise preconditioning group. CONCLUSION: Exercise preconditioning is redounded to lighten the ultrastructure injury of skeletal muscle induced by eccentric exercise and blood indices changes induced by exercise stress. The individual variation of CK and CK-MM is so tremendous that they fit the comparison of intrasubject variability.

Objective To explore optimized method of estimating 131I effective half-life(EHL) in patients with Graves' disease(GD).Methods The EHL was obtained by measuring thyroid radioactive iodine uptake (RAIU) of 126 patients (32 males,94 females,average age (38.47± 11.49) years) with GD at 4,24,48,96,120,144,168,192 and 216 h.Two-sample t test,bivariate correlation analysis and curve estimation were used for statistical analysis.Results The EHL of 126 patients was 0.5-7.5 (4.70±1.46) d.The EHL was 3.0-7.5(5.25±1.01) d and 0.5-6.0(3.24±1.52) d in group A (94 patients,without advanced peak of RAIU) and group B (32 patients,with advanced peak of RAIU),respectively,and the difference between the 2 groups was significant (t =8.499,P＜0.01).The EHL had no significant correlation with RAIU4h/RAIU24h(r=-0.177,P＞0.05) in group A,while it had negative correlation with RAIU4h/RAIU24h in group B (r =-0.724,P＜0.01).In group A,the EHL of patients with RAIU24h/RAIU48h ＞100％ was 3.0-7.5 (5.11 ±0.98) d,the EHL of patients with RAIU24h/RAIU48h ≤ 100％ was 4.0-7.0(5.91±0.94) d (t=3.141,P＜0.01).In group B,when RAIU4h/RAIU24h+RAIU24h/RAIU48h was used as the independent variable in S curve model to estimate the EHL,the result was the best (R2=0.930,F=397.22,both P＜0.01,b0 =-3.038 0,b1 =9.659 4).Conclusions In order to optimally estimate EHL,patients should be grouped according to 131I turnover.In patients with advanced peak of RAIU,the EHL should be calculated by using formula of (RAIU4h/RAIU24h +RAIU24h/RAIU48h).If no advanced peak of RAIU exists,patients should be further divided according to RAIU24h/RAIU48h ＞ 100％ or RAIU24h/RAIU48h ≤100％,and the EHL mean value of each group is taken as the estimated EHL.

OBJECTIVE:To study the protective effect of the injection of puerarin combined with salvianoli acid B(Sal B)on rats with myocardial ischemia reperfusion injury (MIRI). METHODS:62 rats were randomly divided into sham operation group, model group,puerarin group(20 mg/kg)and puerarin(20 mg/kg)-Sal B group(mass ratio of 1:0.5,1:1,1:2,respectively),10 in each group. Except for sham operation group,rats in other groups were reduced for MIRI model. After 180 min of reperfusion, kinase(CK),lactate dehydrogenase(LDH),superoxide dismutase(SOD),malondialdehyde(MDA)in serum and percentage of myocardial infarction size of rats were detected. RESULTS:Compared with sham operation group,CK,LDH,MDA levels in se-rum of rats in model group were obviously increased (P<0.01),SOD level was obviously decreased (P<0.01);and percentage of myocardial infarction size was obviously increased (P<0.01). Compared with model group,CK,LDH,MDA levels in serum of rats in each administration group were decreased(P<0.05 or P<0.01),SOD levels were obviously increased(P<0.05 or P<0.01),and indexes changed the most obviously in puerarin-Sal B group(1:1);percentage of myocardial infarction size was obvi-ously decreased(P<0.01),and the percentage of myocardial infarction sizes in puerarin-Sal B group(1:1)and group(1:2)were less than Puerarin injection group (P<0.01). CONCLUSIONS:Compared with Puerarin injection alone,puerarin combined with Sal B by injection can more effectively inhibit the cardiomyocyte injury and decrease myocardial infarction size after MIRI,with best efficacy when quality ratio is 1:1.

Objective To summarize the clinical experience of laparoscopic cholecystectomy (LC) in the treatment of acute cholecystitis. Methods A total of 93 cases of acute cholecystitis treated by LC from May 2003 to May 2005 was retrospectively reviewed, including 15 cases of preoperative endoscopic retrograde cholangiopancreatography (ERCP) combined with endoscopic sphincterotomy (EST) for common bile duct stones and 6 cases of intraoperative cholangiography. The LC was performed within 48 hours after admission. Results The LC was successfully completed in 91 cases (97.8%), whereas a conversion to open surgery was required in 2 cases (2.2%). The operation time was 35~160 min (mean, 65 min). Postoperatively, biliary leakage occurred in 3 cases (3.2%) and residual stones in the common bile duct were found in 3 cases (3.2%), which were all cured by open surgery combined with ERCP, EST, and endoscopic nasobiliary drainage (ENBD). No iatrogenic injuries happened. Conclusions With proper selection of ERCP and EST, LC for the treatment of acute cholecystitis is feasible and safe. But the incidence of conversions and complications may be high.

HPV DNA were detected in 60 biopsies from women with condyloma acuminata (vulva 47,Vagina 12,cervix 1)by dot blot hybridiztion;physical states of 8 HPV 11 DNA were analyzed by techniques of restriction endonuclease and Southern blot hybridization. HPV 11 was found in 19biopsies of vulva (13 ). vagina (5)and cervix (1),and HPV 16 and 18 in lvaginal and 1 vulvar biopsy respectively. All of HPV 11 DNA were episomal in cells . Our results provided the data for further studying the relationship between HPV and genital cancer in our country.

Objective:To improve the diagnostic accuracy of double contrast radiography of small and micro gastric carcinoma.Methods:The X-ray findings of small gastric carcinoma(9 cases)and micro-gastric carcinoma(3 cases) proved by surgical pathology were analyzed and compared with pathologic finding.1 misdiagnosed cases and 1 missed cases were analyzed.The double contrast films of acute gastric ulcer were reviewed and differentiated with SGC and MGC.Results:8 cases were examined by the double contrast procedure before gastroscopy,4 were diagnosed small gastric carcinoma,1 was diagnosed micro-gastric carcinoma,1 was misdiagnosed ulcer and 1 was missed,1 was suspected of being carcinoma.4 were examined by the double contrast procedure after gastroscopy,2 were small gastric carcinoma and 1 was micro-gastric carcinoma,which accorded with gastroscopy finding.One of micro-gastric carcinoma missed by gastroscopy was detected by DC.Conclusion:The imaging method of double contrast examination is the most effective one in detecting and diagnosing early gastric cancer.The detecting rate should be obviously increased by combining with gastroscopy closely. [